JP2020507312A - 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 - Google Patents

複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 Download PDF

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JP2020507312A
JP2020507312A JP2019539756A JP2019539756A JP2020507312A JP 2020507312 A JP2020507312 A JP 2020507312A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2020507312 A JP2020507312 A JP 2020507312A
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dna
crispr
ctag
sequence
modular
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JP2020507312A5 (cg-RX-API-DMAC7.html
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チャイキンド，ブライアン
ロッサム，ヘンドリク マリヌス ヴァン
ロッサム，ヘンドリク マリヌス ヴァン
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Zymergen Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag

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  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2019539756A 2017-02-10 2018-02-09 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 Withdrawn JP2020507312A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762457493P 2017-02-10 2017-02-10
US62/457,493 2017-02-10
PCT/US2018/017573 WO2018148511A1 (en) 2017-02-10 2018-02-09 A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts

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JP2020507312A true JP2020507312A (ja) 2020-03-12
JP2020507312A5 JP2020507312A5 (cg-RX-API-DMAC7.html) 2021-03-25

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US (2) US11098305B2 (cg-RX-API-DMAC7.html)
EP (1) EP3580337A4 (cg-RX-API-DMAC7.html)
JP (1) JP2020507312A (cg-RX-API-DMAC7.html)
KR (1) KR20190116282A (cg-RX-API-DMAC7.html)
CN (1) CN110312797A (cg-RX-API-DMAC7.html)
CA (1) CA3049989A1 (cg-RX-API-DMAC7.html)
WO (1) WO2018148511A1 (cg-RX-API-DMAC7.html)

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KR20190116282A (ko) 2017-02-10 2019-10-14 지머젠 인코포레이티드 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략
JP2021533773A (ja) * 2018-08-15 2021-12-09 ザイマージェン インコーポレイテッド ハイスループット代謝操作におけるＣＲＩＳＰＲｉの適用
CN113795588B (zh) 2019-04-04 2025-02-25 瑞泽恩制药公司 用于在靶向性载体中无瘢痕引入靶向修饰的方法
CN114555803B (zh) * 2019-10-14 2024-11-29 延世大学校产学协力团 新的前间区序列邻近基序序列以及利用其修饰细胞基因组中靶核酸的方法
KR102178813B1 (ko) 2019-10-18 2020-11-13 주식회사 다우진유전자연구소 Dna 구조체를 적용한 간이 키트
WO2021098709A1 (zh) * 2019-11-18 2021-05-27 中国科学院遗传与发育生物学研究所 衍生自黄杆菌的基因编辑系统
WO2021108324A1 (en) * 2019-11-27 2021-06-03 Technical University Of Denmark Constructs, compositions and methods thereof having improved genome editing efficiency and specificity
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GB202117455D0 (en) * 2021-12-02 2022-01-19 Academisch Ziekenhuis Leiden Method of editing nucleic acid
US20250223620A1 (en) * 2022-04-08 2025-07-10 Glaxosmithkline Intellectual Property Development Limited Novel processes for the production of polynucleotides including oligonucleotides
CN116284436A (zh) * 2022-11-25 2023-06-23 广州美格生物科技有限公司 嵌合体TsCas12a蛋白及其制备方法和应用
CN119432813A (zh) * 2023-12-29 2025-02-14 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用
WO2025165780A1 (en) * 2024-02-01 2025-08-07 The Board Of Trustees Of The Leland Stanford Junior University Combinatorial gene editing and screening

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JP2021533773A (ja) * 2018-08-15 2021-12-09 ザイマージェン インコーポレイテッド ハイスループット代謝操作におけるＣＲＩＳＰＲｉの適用

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US20200123540A1 (en) 2020-04-23
KR20190116282A (ko) 2019-10-14
US11098305B2 (en) 2021-08-24
WO2018148511A1 (en) 2018-08-16
EP3580337A4 (en) 2020-12-02
EP3580337A1 (en) 2019-12-18
CN110312797A (zh) 2019-10-08
US20210371856A1 (en) 2021-12-02
CA3049989A1 (en) 2018-08-16

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