JP2020507312A5 - - Google Patents
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- Publication number
- JP2020507312A5 JP2020507312A5 JP2019539756A JP2019539756A JP2020507312A5 JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5 JP 2019539756 A JP2019539756 A JP 2019539756A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2020507312 A5 JP2020507312 A5 JP 2020507312A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- crispr
- ctag
- digested
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762457493P | 2017-02-10 | 2017-02-10 | |
| US62/457,493 | 2017-02-10 | ||
| PCT/US2018/017573 WO2018148511A1 (en) | 2017-02-10 | 2018-02-09 | A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020507312A JP2020507312A (ja) | 2020-03-12 |
| JP2020507312A5 true JP2020507312A5 (cg-RX-API-DMAC10.html) | 2021-03-25 |
Family
ID=63107838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019539756A Withdrawn JP2020507312A (ja) | 2017-02-10 | 2018-02-09 | 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11098305B2 (cg-RX-API-DMAC10.html) |
| EP (1) | EP3580337A4 (cg-RX-API-DMAC10.html) |
| JP (1) | JP2020507312A (cg-RX-API-DMAC10.html) |
| KR (1) | KR20190116282A (cg-RX-API-DMAC10.html) |
| CN (1) | CN110312797A (cg-RX-API-DMAC10.html) |
| CA (1) | CA3049989A1 (cg-RX-API-DMAC10.html) |
| WO (1) | WO2018148511A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110312797A (zh) | 2017-02-10 | 2019-10-08 | 齐默尔根公司 | 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 |
| KR20210044795A (ko) * | 2018-08-15 | 2021-04-23 | 지머젠 인코포레이티드 | 고 처리량 대사 공학에서 CRISPRi의 응용 |
| AU2020253531C1 (en) | 2019-04-04 | 2022-06-16 | Regeneron Pharmaceuticals, Inc. | Methods for scarless introduction of targeted modifications into targeting vectors |
| KR102421129B1 (ko) * | 2019-10-14 | 2022-07-15 | 연세대학교 산학협력단 | 신규 프로토스페이서 인접 모티프 서열 및 이를 이용한 세포의 유전체에서 표적 핵산을 변형시키는 방법 |
| KR102178813B1 (ko) | 2019-10-18 | 2020-11-13 | 주식회사 다우진유전자연구소 | Dna 구조체를 적용한 간이 키트 |
| CN115052980B (zh) * | 2019-11-18 | 2024-09-10 | 苏州齐禾生科生物科技有限公司 | 衍生自黄杆菌的基因编辑系统 |
| EP4065701A4 (en) * | 2019-11-27 | 2023-11-29 | Danmarks Tekniske Universitet | CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY |
| AU2021230464A1 (en) | 2020-03-03 | 2022-09-01 | Codex Dna, Inc. | Methods for assembling nucleic acids |
| CN113373130B (zh) * | 2021-05-31 | 2023-12-22 | 复旦大学 | Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用 |
| GB202117455D0 (en) * | 2021-12-02 | 2022-01-19 | Academisch Ziekenhuis Leiden | Method of editing nucleic acid |
| TW202405168A (zh) * | 2022-04-08 | 2024-02-01 | 英商葛蘭素史密斯克藍智慧財產發展有限公司 | 製造含有寡核苷酸之聚核苷酸的新穎方法 |
| CN116284436B (zh) * | 2022-11-25 | 2026-04-07 | 广州美格生物科技有限公司 | 嵌合体TsCas12a蛋白及其制备方法和应用 |
| CN119432813A (zh) * | 2023-12-29 | 2025-02-14 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
| WO2025165780A1 (en) * | 2024-02-01 | 2025-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Combinatorial gene editing and screening |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2204913T3 (es) | 1993-04-12 | 2004-05-01 | Northwestern University | Metodo para formacion de oligonucleotidos. |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| ATE362986T1 (de) | 1998-10-30 | 2007-06-15 | Cornell Res Foundation Inc | High fidelity thermostabile ligase und ihre verwendung |
| KR20100133319A (ko) * | 2009-06-11 | 2010-12-21 | 주식회사 툴젠 | 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도 |
| GB2481425A (en) * | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| ES2728782T3 (es) | 2012-05-25 | 2019-10-28 | Univ California | Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| MX380562B (es) | 2012-12-12 | 2025-03-12 | Broad Inst Inc | Modificaciones de sistemas, métodos y composiciones guía optimizadas para la manipulación de secuencias. |
| CN105209621B (zh) | 2012-12-12 | 2021-05-25 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| PT2931898E (pt) | 2012-12-12 | 2016-06-16 | Harvard College | Manipulação e otimização de sistemas, métodos e composições para manipulação de sequências com domínios funcionais |
| KR102955556B1 (ko) | 2012-12-12 | 2026-04-22 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물 |
| CN105408483A (zh) * | 2013-03-15 | 2016-03-16 | 通用医疗公司 | 遗传和表观遗传调节蛋白至特定基因组基因座的rna引导的靶向 |
| US9873907B2 (en) | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
| WO2014197748A2 (en) | 2013-06-05 | 2014-12-11 | Duke University | Rna-guided gene editing and gene regulation |
| ES2777217T3 (es) * | 2013-06-17 | 2020-08-04 | Broad Inst Inc | Suministro, modificación y optimización de sistemas de guía en tándem, métodos y composiciones para la manipulación de secuencias |
| JP6738728B2 (ja) * | 2013-06-17 | 2020-08-19 | ザ・ブロード・インスティテュート・インコーポレイテッド | 肝臓ターゲティングおよび治療のためのＣＲＩＳＰＲ−Ｃａｓ系、ベクターおよび組成物の送達および使用 |
| US10287590B2 (en) | 2014-02-12 | 2019-05-14 | Dna2.0, Inc. | Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
| US9738897B2 (en) * | 2014-06-23 | 2017-08-22 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated DNA assembly |
| US20180002706A1 (en) * | 2014-12-30 | 2018-01-04 | University Of South Florida | Methods and compositions for cloning into large vectors |
| CA3012631A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| US20190330659A1 (en) | 2016-07-15 | 2019-10-31 | Zymergen Inc. | Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase |
| CN109563505A (zh) * | 2016-07-28 | 2019-04-02 | 帝斯曼知识产权资产管理有限公司 | 用于真核细胞的组装系统 |
| CN110312797A (zh) | 2017-02-10 | 2019-10-08 | 齐默尔根公司 | 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 |
| KR20210044795A (ko) | 2018-08-15 | 2021-04-23 | 지머젠 인코포레이티드 | 고 처리량 대사 공학에서 CRISPRi의 응용 |
-
2018
- 2018-02-09 CN CN201880012880.0A patent/CN110312797A/zh active Pending
- 2018-02-09 WO PCT/US2018/017573 patent/WO2018148511A1/en not_active Ceased
- 2018-02-09 CA CA3049989A patent/CA3049989A1/en active Pending
- 2018-02-09 JP JP2019539756A patent/JP2020507312A/ja not_active Withdrawn
- 2018-02-09 EP EP18751852.7A patent/EP3580337A4/en not_active Withdrawn
- 2018-02-09 KR KR1020197022014A patent/KR20190116282A/ko not_active Withdrawn
-
2019
- 2019-08-08 US US16/535,245 patent/US11098305B2/en not_active Expired - Fee Related
-
2021
- 2021-08-03 US US17/392,366 patent/US20210371856A1/en not_active Abandoned
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