CN110312797A - 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 - Google Patents

组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 Download PDF

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CN110312797A
CN110312797A CN201880012880.0A CN201880012880A CN110312797A CN 110312797 A CN110312797 A CN 110312797A CN 201880012880 A CN201880012880 A CN 201880012880A CN 110312797 A CN110312797 A CN 110312797A
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glu
dna
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B·柴金德
H·M·范罗苏姆
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Zymergen Inc
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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CN201880012880.0A 2017-02-10 2018-02-09 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略 Pending CN110312797A (zh)

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US201762457493P 2017-02-10 2017-02-10
US62/457,493 2017-02-10
PCT/US2018/017573 WO2018148511A1 (en) 2017-02-10 2018-02-09 A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts

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CN110312797A true CN110312797A (zh) 2019-10-08

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US (2) US11098305B2 (cg-RX-API-DMAC10.html)
EP (1) EP3580337A4 (cg-RX-API-DMAC10.html)
JP (1) JP2020507312A (cg-RX-API-DMAC10.html)
KR (1) KR20190116282A (cg-RX-API-DMAC10.html)
CN (1) CN110312797A (cg-RX-API-DMAC10.html)
CA (1) CA3049989A1 (cg-RX-API-DMAC10.html)
WO (1) WO2018148511A1 (cg-RX-API-DMAC10.html)

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Publication number Priority date Publication date Assignee Title
CN112703250A (zh) * 2018-08-15 2021-04-23 齐默尔根公司 CRISPRi在高通量代谢工程中的应用
CN116284436A (zh) * 2022-11-25 2023-06-23 广州美格生物科技有限公司 嵌合体TsCas12a蛋白及其制备方法和应用
WO2025139809A1 (zh) * 2023-12-29 2025-07-03 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用

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CN110312797A (zh) 2017-02-10 2019-10-08 齐默尔根公司 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略
AU2020253531C1 (en) 2019-04-04 2022-06-16 Regeneron Pharmaceuticals, Inc. Methods for scarless introduction of targeted modifications into targeting vectors
KR102421129B1 (ko) * 2019-10-14 2022-07-15 연세대학교 산학협력단 신규 프로토스페이서 인접 모티프 서열 및 이를 이용한 세포의 유전체에서 표적 핵산을 변형시키는 방법
KR102178813B1 (ko) 2019-10-18 2020-11-13 주식회사 다우진유전자연구소 Dna 구조체를 적용한 간이 키트
CN115052980B (zh) * 2019-11-18 2024-09-10 苏州齐禾生科生物科技有限公司 衍生自黄杆菌的基因编辑系统
EP4065701A4 (en) * 2019-11-27 2023-11-29 Danmarks Tekniske Universitet CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY
AU2021230464A1 (en) 2020-03-03 2022-09-01 Codex Dna, Inc. Methods for assembling nucleic acids
CN113373130B (zh) * 2021-05-31 2023-12-22 复旦大学 Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用
GB202117455D0 (en) * 2021-12-02 2022-01-19 Academisch Ziekenhuis Leiden Method of editing nucleic acid
TW202405168A (zh) * 2022-04-08 2024-02-01 英商葛蘭素史密斯克藍智慧財產發展有限公司 製造含有寡核苷酸之聚核苷酸的新穎方法
WO2025165780A1 (en) * 2024-02-01 2025-08-07 The Board Of Trustees Of The Leland Stanford Junior University Combinatorial gene editing and screening

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112703250A (zh) * 2018-08-15 2021-04-23 齐默尔根公司 CRISPRi在高通量代谢工程中的应用
CN116284436A (zh) * 2022-11-25 2023-06-23 广州美格生物科技有限公司 嵌合体TsCas12a蛋白及其制备方法和应用
WO2025139809A1 (zh) * 2023-12-29 2025-07-03 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用

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EP3580337A1 (en) 2019-12-18
CA3049989A1 (en) 2018-08-16
US11098305B2 (en) 2021-08-24
WO2018148511A1 (en) 2018-08-16
US20210371856A1 (en) 2021-12-02
KR20190116282A (ko) 2019-10-14
JP2020507312A (ja) 2020-03-12
EP3580337A4 (en) 2020-12-02
US20200123540A1 (en) 2020-04-23

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