JP2019536462A5 - - Google Patents

Info

Publication number

JP2019536462A5

JP2019536462A5 JP2019530105A JP2019530105A JP2019536462A5 JP 2019536462 A5 JP2019536462 A5 JP 2019536462A5 JP 2019530105 A JP2019530105 A JP 2019530105A JP 2019530105 A JP2019530105 A JP 2019530105A JP 2019536462 A5 JP2019536462 A5 JP 2019536462A5

Authority

JP

Japan

Prior art keywords

nucleic acid

acid molecule

genome editing

cas9 protein

sequence

Prior art date

2017-12-05

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 150000007523 nucleic acids Chemical class 0.000 claims 90
• 108020004707 nucleic acids Proteins 0.000 claims 65
• 102000039446 nucleic acids Human genes 0.000 claims 65
• 108091033409 CRISPR Proteins 0.000 claims 58
• 238000010362 genome editing Methods 0.000 claims 40
• 150000001413 amino acids Chemical class 0.000 claims 29
• 108090000623 proteins and genes Proteins 0.000 claims 29
• 108020005004 Guide RNA Proteins 0.000 claims 27
• 235000018102 proteins Nutrition 0.000 claims 25
• 102000004169 proteins and genes Human genes 0.000 claims 25
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 24
• 235000001014 amino acid Nutrition 0.000 claims 24
• 238000003780 insertion Methods 0.000 claims 22
• 230000037431 insertion Effects 0.000 claims 22
• 239000002773 nucleotide Substances 0.000 claims 12
• 125000003729 nucleotide group Chemical group 0.000 claims 12
• 230000001052 transient effect Effects 0.000 claims 8
• 108091026890 Coding region Proteins 0.000 claims 6
• 239000013598 vector Substances 0.000 claims 6
• 101710163270 Nuclease Proteins 0.000 claims 5
• 230000000694 effects Effects 0.000 claims 5
• 230000008685 targeting Effects 0.000 claims 5
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 4
• DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 4
• 230000000295 complement effect Effects 0.000 claims 4
• 239000013603 viral vector Substances 0.000 claims 4
• 230000014509 gene expression Effects 0.000 claims 3
• 229920001184 polypeptide Polymers 0.000 claims 3
• 102000004196 processed proteins & peptides Human genes 0.000 claims 3
• 108090000765 processed proteins & peptides Proteins 0.000 claims 3
• 238000006467 substitution reaction Methods 0.000 claims 3
• 108020004705 Codon Proteins 0.000 claims 2
• 239000004471 Glycine Substances 0.000 claims 2
• 230000003834 intracellular effect Effects 0.000 claims 2
• 238000000034 method Methods 0.000 claims 2
• 125000006850 spacer group Chemical group 0.000 claims 2
• 241000702421 Dependoparvovirus Species 0.000 claims 1
• QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 1
• KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 1
• 101000897880 Spiroplasma virus SpV1-R8A2 B Putative capsid protein ORF9 Proteins 0.000 claims 1
• KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 1
• 239000002253 acid Substances 0.000 claims 1
• 235000004279 alanine Nutrition 0.000 claims 1
• 230000001413 cellular effect Effects 0.000 claims 1
• 238000010363 gene targeting Methods 0.000 claims 1
• 238000012986 modification Methods 0.000 claims 1
• 230000004048 modification Effects 0.000 claims 1
• 229960000856 protein c Drugs 0.000 claims 1
• 230000010474 transient expression Effects 0.000 claims 1
• 239000004474 valine Substances 0.000 claims 1