CN111757889A - 新型CRISPR/Cas12f酶和系统 - Google Patents

新型CRISPR/Cas12f酶和系统 Download PDF

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CN111757889A
CN111757889A CN201980014560.3A CN201980014560A CN111757889A CN 111757889 A CN111757889 A CN 111757889A CN 201980014560 A CN201980014560 A CN 201980014560A CN 111757889 A CN111757889 A CN 111757889A
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sequence
nucleic acid
protein
cell
acid molecule
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CN111757889B (zh
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赖锦盛
周英思
朱金洁
易飞
张湘博
赵海铭
宋伟彬
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China Agricultural University
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China Agricultural University
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Abstract

本发明属于核酸编辑领域，特别是规律成簇的间隔短回文重复(CRISPR)技术领域。具体而言，本发明提供了Cas效应蛋白、包含此类蛋白的融合蛋白以及编码它们的核酸分子，还提供了包含上述蛋白或核酸分子的用于核酸编辑(例如，基因或基因组编辑)的复合物和组合物，以及包含上述蛋白的用于核酸编辑(例如，基因或基因组编辑)的方法。

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PCT国内申请，说明书已公开。

Claims (50)

  1. PCT国内申请，权利要求书已公开。
CN201980014560.3A 2018-10-29 2019-10-29 新型CRISPR/Cas12f酶和系统 Active CN111757889B (zh)

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CN113881652A (zh) * 2020-11-11 2022-01-04 山东舜丰生物科技有限公司 新型Cas酶和系统以及应用
CN113897416A (zh) * 2021-12-09 2022-01-07 上海科技大学 一种CRISPR/Cas12f的检测体系及其应用
CN114277015A (zh) * 2021-03-16 2022-04-05 山东舜丰生物科技有限公司 新型crispr酶以及应用
CN114292831A (zh) * 2021-02-03 2022-04-08 山东舜丰生物科技有限公司 新型Cas酶以及应用
CN114410609A (zh) * 2022-03-29 2022-04-29 舜丰生物科技(海南)有限公司 一种活性提高的Cas蛋白以及应用
CN114438055A (zh) * 2021-10-26 2022-05-06 山东舜丰生物科技有限公司 新型的crispr酶和系统以及应用
CN114480383A (zh) * 2021-06-08 2022-05-13 山东舜丰生物科技有限公司 一种具有碱基突变的同向重复序列及其应用
CN114517190A (zh) * 2021-02-05 2022-05-20 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
CN114672473A (zh) * 2022-05-31 2022-06-28 舜丰生物科技(海南)有限公司 一种优化的Cas蛋白及其应用
CN116751763A (zh) * 2023-05-08 2023-09-15 珠海舒桐医疗科技有限公司 一种Cpf1蛋白、V型基因编辑系统及应用
WO2023173682A1 (zh) * 2022-03-18 2023-09-21 山东舜丰生物科技有限公司 一种优化的Cas蛋白及其应用
WO2023174249A1 (zh) * 2022-03-18 2023-09-21 山东舜丰生物科技有限公司 活性改善的Cas蛋白及其应用
WO2023202116A1 (zh) * 2022-04-20 2023-10-26 山东舜丰生物科技有限公司 Cas酶和系统以及应用
WO2023231456A1 (zh) * 2022-05-31 2023-12-07 山东舜丰生物科技有限公司 一种优化的Cas蛋白及其应用
CN117568311A (zh) * 2023-11-16 2024-02-20 广西科学院 一种用于精准基因编辑的工程化crispr酶和系统及其应用
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CN114015674B (zh) * 2021-11-02 2024-08-30 辉大(上海)生物科技有限公司 一种CRISPR-Cas12i系统
CN114107370A (zh) * 2021-12-03 2022-03-01 山东舜丰生物科技有限公司 利用Cas12i在大豆中进行基因编辑的方法
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CN116987693A (zh) * 2022-04-25 2023-11-03 上海科技大学 一种优化的CRISPR/SpCas12f1系统、工程化向导RNA及其应用
WO2023208000A1 (en) * 2022-04-25 2023-11-02 Huidagene Therapeutics Co., Ltd. Novel crispr-cas12f systems and uses thereof
CN114634972B (zh) * 2022-05-19 2022-08-26 舜丰生物科技(海南)有限公司 利用Cas酶进行核酸检测的方法
CN114921439B (zh) * 2022-06-16 2024-04-26 尧唐(上海)生物科技有限公司 CRISPR-Cas效应子蛋白、其基因编辑系统及应用
CN117050971A (zh) * 2022-08-08 2023-11-14 山东舜丰生物科技有限公司 Cas突变蛋白及其应用
CN115975986B (zh) * 2022-08-22 2023-08-08 山东舜丰生物科技有限公司 突变的Cas12j蛋白及其应用
WO2024091775A1 (en) * 2022-10-24 2024-05-02 Inari Agriculture Technology, Inc. Variant rna-guided cas12f4 nucleases and dna binding proteins
CN117230041A (zh) * 2022-10-25 2023-12-15 山东舜丰生物科技有限公司 编辑活性提高的Cas蛋白及其应用
CN117844782B (zh) * 2024-03-06 2024-05-31 崖州湾国家实验室 靶向范围广的基因编辑核酸酶及其在核酸检测中的应用

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