CN111770992A - CRISPR-Cas12j酶和系统 - Google Patents

CRISPR-Cas12j酶和系统 Download PDF

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CN111770992A
CN111770992A CN201980014005.0A CN201980014005A CN111770992A CN 111770992 A CN111770992 A CN 111770992A CN 201980014005 A CN201980014005 A CN 201980014005A CN 111770992 A CN111770992 A CN 111770992A
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sequence
protein
nucleic acid
seq
cell
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CN111770992B (zh
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赖锦盛
周英思
李英男
张继红
王莹莹
吕梦璐
张湘博
赵海铭
宋伟彬
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China Agricultural University
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China Agricultural University
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Abstract

提供一种Cas效应蛋白，包含此类蛋白的融合蛋白，以及其编码核酸分子。还提供用于核酸编辑的复合物和组合物，例如基因或基因组编辑的复合物和组合物，其包含Cas效应蛋白或融合蛋白，或其编码核酸分子。还提供用于核酸编辑的方法，例如基因或基因组编辑的方法，其使用Cas效应蛋白或融合蛋白。

Description

PCT国内申请，说明书已公开。

Claims (47)

  1. PCT国内申请，权利要求书已公开。
CN201980014005.0A 2018-11-15 2019-11-15 CRISPR-Cas12j酶和系统 Active CN111770992B (zh)

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CN201811355943 2018-11-15
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US (1) US20220002691A1 (zh)
EP (1) EP3882345A4 (zh)
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KR (1) KR20210142586A (zh)
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AU (1) AU2019381258B2 (zh)
BR (1) BR112021009330A2 (zh)
CA (1) CA3120432A1 (zh)
IL (1) IL283169A (zh)
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113234795A (zh) * 2021-04-15 2021-08-10 山东舜丰生物科技有限公司 利用Cas蛋白进行核酸检测的方法
CN113717962A (zh) * 2021-09-10 2021-11-30 武汉艾迪晶生物科技有限公司 用于水稻基因编辑的CasΦ-2蛋白及其表达盒子和表达载体
CN113881652A (zh) * 2020-11-11 2022-01-04 山东舜丰生物科技有限公司 新型Cas酶和系统以及应用
CN114517190A (zh) * 2021-02-05 2022-05-20 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
CN115975986A (zh) * 2022-08-22 2023-04-18 山东舜丰生物科技有限公司 突变的Cas12j蛋白及其应用
CN115992115A (zh) * 2021-10-26 2023-04-21 山东舜丰生物科技有限公司 新型的crispr酶和系统以及应用
WO2023202116A1 (zh) * 2022-04-20 2023-10-26 山东舜丰生物科技有限公司 Cas酶和系统以及应用

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2020231380A1 (en) 2019-03-07 2021-09-23 The Regents Of The University Of California CRISPR-Cas effector polypeptides and methods of use thereof
AU2020397041A1 (en) * 2019-12-04 2022-06-09 Arbor Biotechnologies, Inc. Compositions comprising a nuclease and uses thereof
EP4139447A4 (en) * 2020-04-20 2024-05-29 Univ California CRISPR SYSTEMS IN PLANTS
CN111996236B (zh) * 2020-05-29 2021-06-29 山东舜丰生物科技有限公司 基于crispr技术进行靶核酸检测的方法
CN111690773B (zh) * 2020-06-17 2021-08-20 山东舜丰生物科技有限公司 利用新型Cas酶进行目标核酸检测的方法和系统
US20240093228A1 (en) * 2021-01-22 2024-03-21 Arbor Biotechnologies, Inc. Compositions comprising a nuclease and uses thereof
CA3219005A1 (en) * 2021-06-02 2022-12-08 Guillermo Montoya Mutant cas12j endonucleases
WO2023143342A1 (zh) * 2022-01-29 2023-08-03 山东舜丰生物科技有限公司 Cas酶和系统以及应用
CN116987693A (zh) * 2022-04-25 2023-11-03 上海科技大学 一种优化的CRISPR/SpCas12f1系统、工程化向导RNA及其应用
CN116716277A (zh) * 2022-07-07 2023-09-08 山东舜丰生物科技有限公司 Cas酶及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105899657A (zh) * 2013-12-12 2016-08-24 布罗德研究所有限公司 用于改变基因产物表达的crispr-cas系统和方法、结构信息以及诱导型模块化cas酶
WO2018035387A1 (en) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140066379A1 (en) * 2012-08-30 2014-03-06 Body Organ Biomedical Corp. Recombinant vector, transgenic fish egg using the same and biomaterial using the same
JP5774657B2 (ja) * 2013-10-04 2015-09-09 国立大学法人京都大学 エレクトロポレーションを利用した哺乳類の遺伝子改変方法
CN107406838A (zh) * 2014-11-06 2017-11-28 纳幕尔杜邦公司 Rna引导的内切核酸酶向细胞中的肽介导的递送
WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CN105296518A (zh) * 2015-12-01 2016-02-03 中国农业大学 一种用于CRISPR/Cas9技术的同源臂载体构建方法
CN106845151B (zh) * 2015-12-07 2019-03-26 中国农业大学 CRISPR-Cas9系统sgRNA作用靶点的筛选方法及装置
CN115216459A (zh) * 2015-12-29 2022-10-21 孟山都技术公司 新型crispr相关转座酶及其用途
CA3026110A1 (en) * 2016-04-19 2017-11-02 The Broad Institute, Inc. Novel crispr enzymes and systems
KR20190019168A (ko) * 2016-06-17 2019-02-26 더 브로드 인스티튜트, 인코퍼레이티드 제vi형 crispr 오솔로그 및 시스템
CN107784200B (zh) * 2016-08-26 2020-11-06 深圳华大生命科学研究院 一种筛选新型CRISPR-Cas系统的方法和装置
CN106978428A (zh) * 2017-03-15 2017-07-25 上海吐露港生物科技有限公司 一种Cas蛋白特异结合靶标DNA、调控靶标基因转录的方法及试剂盒
CN112004932B (zh) * 2018-04-20 2023-01-10 中国农业大学 一种CRISPR/Cas效应蛋白及系统
CN112020560B (zh) * 2018-04-25 2024-02-23 中国农业大学 一种RNA编辑的CRISPR/Cas效应蛋白及系统
WO2019214604A1 (zh) * 2018-05-07 2019-11-14 中国农业大学 CRISPR/Cas效应蛋白及系统
US10934536B2 (en) * 2018-12-14 2021-03-02 Pioneer Hi-Bred International, Inc. CRISPR-CAS systems for genome editing
AU2020231380A1 (en) * 2019-03-07 2021-09-23 The Regents Of The University Of California CRISPR-Cas effector polypeptides and methods of use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105899657A (zh) * 2013-12-12 2016-08-24 布罗德研究所有限公司 用于改变基因产物表达的crispr-cas系统和方法、结构信息以及诱导型模块化cas酶
WO2018035387A1 (en) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113881652A (zh) * 2020-11-11 2022-01-04 山东舜丰生物科技有限公司 新型Cas酶和系统以及应用
CN113881652B (zh) * 2020-11-11 2022-11-22 山东舜丰生物科技有限公司 新型Cas酶和系统以及应用
CN114517190B (zh) * 2021-02-05 2022-12-23 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
CN114517190A (zh) * 2021-02-05 2022-05-20 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
WO2022166895A1 (zh) * 2021-02-05 2022-08-11 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
CN113234795A (zh) * 2021-04-15 2021-08-10 山东舜丰生物科技有限公司 利用Cas蛋白进行核酸检测的方法
CN113234795B (zh) * 2021-04-15 2023-02-24 山东舜丰生物科技有限公司 利用Cas蛋白进行核酸检测的方法
CN113717962A (zh) * 2021-09-10 2021-11-30 武汉艾迪晶生物科技有限公司 用于水稻基因编辑的CasΦ-2蛋白及其表达盒子和表达载体
CN115992115A (zh) * 2021-10-26 2023-04-21 山东舜丰生物科技有限公司 新型的crispr酶和系统以及应用
CN115992115B (zh) * 2021-10-26 2023-09-01 山东舜丰生物科技有限公司 新型的crispr酶和系统以及应用
WO2023202116A1 (zh) * 2022-04-20 2023-10-26 山东舜丰生物科技有限公司 Cas酶和系统以及应用
CN115975986A (zh) * 2022-08-22 2023-04-18 山东舜丰生物科技有限公司 突变的Cas12j蛋白及其应用
CN115975986B (zh) * 2022-08-22 2023-08-08 山东舜丰生物科技有限公司 突变的Cas12j蛋白及其应用
WO2024040874A1 (zh) * 2022-08-22 2024-02-29 山东舜丰生物科技有限公司 突变的Cas12j蛋白及其应用

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US20220002691A1 (en) 2022-01-06
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CN113462671A (zh) 2021-10-01
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