CN112105728A - CRISPR/Cas效应蛋白及系统 - Google Patents

CRISPR/Cas效应蛋白及系统 Download PDF

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CN112105728A
CN112105728A CN201980030881.2A CN201980030881A CN112105728A CN 112105728 A CN112105728 A CN 112105728A CN 201980030881 A CN201980030881 A CN 201980030881A CN 112105728 A CN112105728 A CN 112105728A
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sequence
protein
nucleic acid
seq
cell
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CN112105728B (zh
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赖锦盛
周英思
朱金洁
张湘博
赵海铭
宋伟彬
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China Agricultural University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12N9/22—Ribonucleases RNAses, DNAses

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Abstract

涉及核酸编辑领域，特别是规律成簇的间隔短回文重复(CRISPR)技术领域。一种Cas效应蛋白，包含此类蛋白的融合蛋白，以及编码它们的核酸分子。提供了用于核酸编辑(例如，基因或基因组编辑)的复合物和组合物，其包含上述蛋白或融合蛋白，或编码它们的核酸分子。用于核酸编辑(例如，基因或基因组编辑)的方法，其使用包含上述的蛋白或融合蛋白。

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PCT国内申请，说明书已公开。

Claims (35)

  1. PCT国内申请，权利要求书已公开。
CN201980030881.2A 2018-05-07 2019-05-07 CRISPR/Cas效应蛋白及系统 Active CN112105728B (zh)

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CN2018104266661 2018-05-07
CN201810426666 2018-05-07
PCT/CN2019/085826 WO2019214604A1 (zh) 2018-05-07 2019-05-07 CRISPR/Cas效应蛋白及系统

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Cited By (2)

* Cited by examiner, † Cited by third party
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WO2022266849A1 (zh) * 2021-06-22 2022-12-29 中国科学院脑科学与智能技术卓越创新中心 新型CRISPR-Cas13蛋白的筛选及其应用
CN116286742A (zh) * 2022-09-29 2023-06-23 隆平生物技术(海南)有限公司 CasD蛋白、CRISPR/CasD基因编辑系统及其在植物基因编辑中的应用

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BR112020002647A2 (pt) * 2017-08-09 2020-08-18 Benson Hill, Inc. composições e métodos para modificação de genomas
MX2021005723A (es) * 2018-11-15 2021-09-23 Univ China Agricultural Enzima y sistema crispr-cas12j.
CA3130789A1 (en) * 2019-03-07 2020-09-10 The Regents Of The University Of California Crispr-cas effector polypeptides and methods of use thereof
US20230045187A1 (en) * 2019-12-04 2023-02-09 Arbor Biotechnologies, Inc. Compositions comprising a nuclease and uses thereof
CA3179130A1 (en) 2020-06-04 2021-12-09 Lior IZHAR Novel omni-59, 61, 67, 76, 79, 80, 81, and 82 crispr nucleases
CN114277015B (zh) * 2021-03-16 2023-12-15 山东舜丰生物科技有限公司 Crispr酶以及应用
CN115261359B (zh) * 2021-05-21 2023-06-30 山东舜丰生物科技有限公司 一种新型crispr酶和系统以及应用

Citations (2)

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US20140349405A1 (en) * 2013-05-22 2014-11-27 Wisconsin Alumni Research Foundation Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis
US20160102322A1 (en) * 2014-10-09 2016-04-14 Life Technologies Corporation Crispr oligonucleotides and gene editing

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CN105907785B (zh) * 2016-05-05 2020-02-07 苏州吉玛基因股份有限公司 化学合成的crRNA用于CRISPR/Cpf1系统在基因编辑中的应用
CN107784200B (zh) * 2016-08-26 2020-11-06 深圳华大生命科学研究院 一种筛选新型CRISPR-Cas系统的方法和装置
EA201990860A1 (ru) * 2016-09-30 2019-10-31 Рнк-направляемые модифицирующие нуклеиновые кислоты ферменты и способы их применения

Patent Citations (2)

* Cited by examiner, † Cited by third party
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US20140349405A1 (en) * 2013-05-22 2014-11-27 Wisconsin Alumni Research Foundation Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis
US20160102322A1 (en) * 2014-10-09 2016-04-14 Life Technologies Corporation Crispr oligonucleotides and gene editing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WINSTON X. YAN等: "Cas13d is a compact RNA-targeting type VI CRISPR effector positively modulated by a WYL domain-containing accessory protein", 《MOL CELL.》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022266849A1 (zh) * 2021-06-22 2022-12-29 中国科学院脑科学与智能技术卓越创新中心 新型CRISPR-Cas13蛋白的筛选及其应用
CN116286742A (zh) * 2022-09-29 2023-06-23 隆平生物技术(海南)有限公司 CasD蛋白、CRISPR/CasD基因编辑系统及其在植物基因编辑中的应用
CN116286742B (zh) * 2022-09-29 2023-11-17 隆平生物技术(海南)有限公司 CasD蛋白、CRISPR/CasD基因编辑系统及其在植物基因编辑中的应用

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WO2019214604A1 (zh) 2019-11-14

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