CA3046076A1 - Systems and methods for one-shot guide rna (ogrna) targeting of endogenous and source dna - Google Patents
Systems and methods for one-shot guide rna (ogrna) targeting of endogenous and source dna Download PDFInfo
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- CA3046076A1 CA3046076A1 CA3046076A CA3046076A CA3046076A1 CA 3046076 A1 CA3046076 A1 CA 3046076A1 CA 3046076 A CA3046076 A CA 3046076A CA 3046076 A CA3046076 A CA 3046076A CA 3046076 A1 CA3046076 A1 CA 3046076A1
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Classifications
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/10—Vectors comprising a non-peptidic targeting moiety
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- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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| PCT/US2017/064720 WO2018106693A1 (en) | 2016-12-05 | 2017-12-05 | SYSTEMS AND METHODS FOR ONE-SHOT GUIDE RNA (ogRNA) TARGETING OF ENDOGENOUS AND SOURCE DNA |
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| CA3001711A1 (en) * | 2015-10-30 | 2017-05-04 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating herpes simplex virus |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| US20190153440A1 (en) | 2017-11-21 | 2019-05-23 | Casebia Therapeutics Llp | Materials and methods for treatment of autosomal dominant retinitis pigmentosa |
| WO2019118875A1 (en) * | 2017-12-15 | 2019-06-20 | Ou Li | Crispr-mediated genome editing with vectors |
| CA3104856A1 (en) | 2018-06-29 | 2020-01-02 | Editas Medicine, Inc. | Synthetic guide molecules, compositions and methods relating thereto |
| WO2020055748A1 (en) * | 2018-09-10 | 2020-03-19 | The Regents Of The University Of California | Modulators of cas9 polypeptides and methods of use thereof |
| WO2020176552A1 (en) | 2019-02-25 | 2020-09-03 | Editas Medicine, Inc. | Crispr/rna-guided nuclease-related methods and compositions for treating rho-associated autosomal-dominant retinitis pigmentosa (adrp) |
| WO2020186059A2 (en) * | 2019-03-12 | 2020-09-17 | Crispr Therapeutics Ag | Novel high fidelity rna-programmable endonuclease systems and uses thereof |
| WO2020264254A1 (en) * | 2019-06-28 | 2020-12-30 | Crispr Therapeutics Ag | Materials and methods for controlling gene editing |
| WO2021053582A1 (en) * | 2019-09-18 | 2021-03-25 | Crispr Therapeutics Ag | All-in-one self inactivating crispr vectors |
| WO2021062201A1 (en) * | 2019-09-25 | 2021-04-01 | Spotlight Therapeutics | Compositions and methods for nucleoprotein targeting and expression |
| US20230203220A1 (en) * | 2020-05-29 | 2023-06-29 | Regents Of The University Of Minnesota | Copolymers for intracellular therapeutic nucleic acid payload delivery |
| CA3200815A1 (en) * | 2020-12-03 | 2022-06-09 | Benjamin OAKES | Compositions and methods for the targeting of bcl11a |
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| WO2025179057A1 (en) * | 2024-02-21 | 2025-08-28 | The Regents Of The University Of California | In vivo modification of cell genomes |
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| SG10201804973TA (en) * | 2013-12-12 | 2018-07-30 | Broad Inst Inc | Compositions and Methods of Use of Crispr-Cas Systems in Nucleotide Repeat Disorders |
| EP3553176A1 (en) | 2014-03-10 | 2019-10-16 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10) |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| KR102598856B1 (ko) * | 2015-03-03 | 2023-11-07 | 더 제너럴 하스피탈 코포레이션 | 변경된 PAM 특이성을 갖는 조작된 CRISPR-Cas9 뉴클레아제 |
| WO2016176404A1 (en) * | 2015-04-30 | 2016-11-03 | The Brigham And Women's Hospital, Inc. | Methods and kits for cloning-free genome editing |
| US20180291372A1 (en) * | 2015-05-14 | 2018-10-11 | Massachusetts Institute Of Technology | Self-targeting genome editing system |
| CN107849547B (zh) | 2015-05-16 | 2022-04-19 | 建新公司 | 深内含子突变的基因编辑 |
| US11530421B2 (en) | 2016-02-01 | 2022-12-20 | The Regents Of The University Of California | Self-inactivating endonuclease-encoding nucleic acids and methods of using the same |
| WO2017173004A1 (en) * | 2016-03-30 | 2017-10-05 | Mikuni Takayasu | A method for in vivo precise genome editing |
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- 2017-12-05 US US15/832,567 patent/US9963719B1/en active Active
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2018
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2019
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2021
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| AU2017373797B2 (en) | 2024-08-29 |
| US20180201956A1 (en) | 2018-07-19 |
| US12264331B2 (en) | 2025-04-01 |
| US9963719B1 (en) | 2018-05-08 |
| US20220017927A1 (en) | 2022-01-20 |
| US10006054B1 (en) | 2018-06-26 |
| US11028411B2 (en) | 2021-06-08 |
| US10494649B2 (en) | 2019-12-03 |
| US20200056208A1 (en) | 2020-02-20 |
| KR20190088555A (ko) | 2019-07-26 |
| JP7272952B2 (ja) | 2023-05-12 |
| US20180251792A1 (en) | 2018-09-06 |
| JP2023052089A (ja) | 2023-04-11 |
| MX2024002327A (es) | 2024-03-07 |
| US11692205B2 (en) | 2023-07-04 |
| EP3548614A1 (en) | 2019-10-09 |
| MX2019006475A (es) | 2019-09-26 |
| CN110168084A (zh) | 2019-08-23 |
| KR102604903B1 (ko) | 2023-11-21 |
| JP2019536462A (ja) | 2019-12-19 |
| WO2018106693A1 (en) | 2018-06-14 |
| AU2017373797A1 (en) | 2019-06-20 |
| US20230416787A1 (en) | 2023-12-28 |
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