JP2019528726A5 - - Google Patents
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- JP2019528726A5 JP2019528726A5 JP2019514212A JP2019514212A JP2019528726A5 JP 2019528726 A5 JP2019528726 A5 JP 2019528726A5 JP 2019514212 A JP2019514212 A JP 2019514212A JP 2019514212 A JP2019514212 A JP 2019514212A JP 2019528726 A5 JP2019528726 A5 JP 2019528726A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- oligonucleotide
- moiety
- quenching
- tag portion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 150000007523 nucleic acids Chemical class 0.000 claims 62
- 238000010791 quenching Methods 0.000 claims 53
- 230000000171 quenching effect Effects 0.000 claims 48
- 108091034117 Oligonucleotide Proteins 0.000 claims 43
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 41
- 239000002751 oligonucleotide probe Substances 0.000 claims 41
- 238000000137 annealing Methods 0.000 claims 34
- 108020004707 nucleic acids Proteins 0.000 claims 25
- 102000039446 nucleic acids Human genes 0.000 claims 25
- 239000002773 nucleotide Substances 0.000 claims 23
- 125000003729 nucleotide group Chemical group 0.000 claims 23
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 21
- 238000000034 method Methods 0.000 claims 18
- 238000003752 polymerase chain reaction Methods 0.000 claims 17
- 101710163270 Nuclease Proteins 0.000 claims 12
- 239000013615 primer Substances 0.000 claims 12
- 108020004635 Complementary DNA Proteins 0.000 claims 11
- 230000000295 complement effect Effects 0.000 claims 11
- 238000003776 cleavage reaction Methods 0.000 claims 10
- 230000007017 scission Effects 0.000 claims 10
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 8
- 238000012986 modification Methods 0.000 claims 8
- 230000004048 modification Effects 0.000 claims 8
- 239000000523 sample Substances 0.000 claims 8
- 230000000694 effects Effects 0.000 claims 6
- 238000000926 separation method Methods 0.000 claims 5
- IVWWFWFVSWOTLP-YVZVNANGSA-N (3'as,4r,7'as)-2,2,2',2'-tetramethylspiro[1,3-dioxolane-4,6'-4,7a-dihydro-3ah-[1,3]dioxolo[4,5-c]pyran]-7'-one Chemical class C([C@@H]1OC(O[C@@H]1C1=O)(C)C)O[C@]21COC(C)(C)O2 IVWWFWFVSWOTLP-YVZVNANGSA-N 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 3
- 238000006243 chemical reaction Methods 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 230000036961 partial effect Effects 0.000 claims 2
- 239000003155 DNA primer Substances 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 230000002452 interceptive effect Effects 0.000 claims 1
- 238000002844 melting Methods 0.000 claims 1
- 230000008018 melting Effects 0.000 claims 1
- 230000002441 reversible effect Effects 0.000 claims 1
- 230000001629 suppression Effects 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662395325P | 2016-09-15 | 2016-09-15 | |
| US62/395,325 | 2016-09-15 | ||
| US201662435595P | 2016-12-16 | 2016-12-16 | |
| US62/435,595 | 2016-12-16 | ||
| US201762536871P | 2017-07-25 | 2017-07-25 | |
| US62/536,871 | 2017-07-25 | ||
| PCT/EP2017/073293 WO2018050828A1 (en) | 2016-09-15 | 2017-09-15 | Methods for performing multiplexed real-time pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019528726A JP2019528726A (ja) | 2019-10-17 |
| JP2019528726A5 true JP2019528726A5 (OSRAM) | 2020-10-22 |
| JP7063886B2 JP7063886B2 (ja) | 2022-05-09 |
Family
ID=59901522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019514294A Active JP7221199B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスｐｃｒの実施方法 |
| JP2019514212A Active JP7063886B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスリアルタイムｐｃｒを実施する方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019514294A Active JP7221199B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスｐｃｒの実施方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US11034997B2 (OSRAM) |
| EP (2) | EP3512960B1 (OSRAM) |
| JP (2) | JP7221199B2 (OSRAM) |
| KR (2) | KR102468633B1 (OSRAM) |
| CN (2) | CN109689889A (OSRAM) |
| AU (2) | AU2017328613B2 (OSRAM) |
| CA (2) | CA3033250C (OSRAM) |
| DK (1) | DK3512959T3 (OSRAM) |
| ES (2) | ES2893411T3 (OSRAM) |
| WO (2) | WO2018050828A1 (OSRAM) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022269023A1 (en) | 2021-06-25 | 2022-12-29 | F. Hoffmann-La Roche Ag | Methods for performing temperature multiplexed pcr with increased sensitivity |
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| RU2665631C2 (ru) * | 2017-01-25 | 2018-09-03 | Общество с ограниченной ответственностью "Секвойя Дженетикс" | Способ специфической идентификации последовательностей днк |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| WO2019144107A1 (en) | 2018-01-22 | 2019-07-25 | Luminex Corporation | Methods and compositions for discrete melt analysis |
| US20190292596A1 (en) | 2018-03-21 | 2019-09-26 | Roche Molecular Systems, Inc. | Modified nucleoside phosphates with high thermal stability |
| ES3010286T3 (en) | 2018-04-20 | 2025-04-02 | Seegene Inc | Method for detecting a plurality of target nucleic acid sequences in sample |
| US11384376B2 (en) | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| KR102796204B1 (ko) | 2018-08-09 | 2025-04-14 | 스피디엑스 피티와이 리미티드 | 핵산의 다중 검출 |
| US11371080B2 (en) | 2018-11-30 | 2022-06-28 | Luminex Corporation | Methods and probes for performing PCR with melt analysis for increased multiplexing |
| US20220298555A1 (en) * | 2018-12-20 | 2022-09-22 | Roche Molecular Systems, Inc. | Detection of target nucleic acid by solid-phase molography |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| JP2023505327A (ja) | 2019-12-09 | 2023-02-08 | エフ．ホフマン－ラ ロシュ アーゲー | ジカチオン性蛍光色素 |
| CN111534518B (zh) * | 2020-05-18 | 2021-07-23 | 纳昂达(南京)生物科技有限公司 | 通用封闭序列及其应用 |
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| CN112195221B (zh) * | 2020-09-13 | 2021-08-06 | 武汉纳诺德医疗科技有限公司 | 一种多温度单荧光通道多重实时荧光定量pcr仪及其检测方法 |
| CA3192943A1 (en) * | 2020-09-16 | 2022-03-24 | Nanostring Technologies, Inc. | Chemical compositions and methods of using the same |
| CN116601308A (zh) | 2020-12-22 | 2023-08-15 | 豪夫迈·罗氏有限公司 | 使用大斯托克斯位移荧光染料进行多重实时pcr的方法 |
| WO2023056345A1 (en) * | 2021-09-30 | 2023-04-06 | Gen-Probe Incorporated | Temperature-selectable fret cassette signaling |
| JP2025521757A (ja) | 2022-06-28 | 2025-07-10 | エフ． ホフマン－ラ ロシュ アーゲー | 大ストークスシフトを有する蛍光色素 |
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| WO2024234030A1 (en) * | 2023-05-15 | 2024-11-21 | Speedx Pty Ltd | Target detection using temperature controlled probes |
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| WO2025141105A1 (en) | 2023-12-27 | 2025-07-03 | bioMérieux | Specific probes for the detection of nucleic acids, methods and uses |
| EP4606908A1 (en) * | 2024-02-21 | 2025-08-27 | Roche Diagnostics GmbH | Method of increasing the number of usable channels in multiplex pcr |
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-
2017
- 2017-09-15 ES ES17768765T patent/ES2893411T3/es active Active
- 2017-09-15 JP JP2019514294A patent/JP7221199B2/ja active Active
- 2017-09-15 US US15/705,821 patent/US11034997B2/en active Active
- 2017-09-15 JP JP2019514212A patent/JP7063886B2/ja active Active
- 2017-09-15 US US15/705,772 patent/US11028433B2/en active Active
- 2017-09-15 CA CA3033250A patent/CA3033250C/en active Active
- 2017-09-15 DK DK17768765.4T patent/DK3512959T3/da active
- 2017-09-15 WO PCT/EP2017/073293 patent/WO2018050828A1/en not_active Ceased
- 2017-09-15 ES ES17768768T patent/ES2933176T3/es active Active
- 2017-09-15 CN CN201780056599.2A patent/CN109689889A/zh active Pending
- 2017-09-15 WO PCT/EP2017/073288 patent/WO2018050824A1/en not_active Ceased
- 2017-09-15 CA CA3036119A patent/CA3036119C/en active Active
- 2017-09-15 EP EP17768768.8A patent/EP3512960B1/en active Active
- 2017-09-15 AU AU2017328613A patent/AU2017328613B2/en active Active
- 2017-09-15 KR KR1020197007697A patent/KR102468633B1/ko active Active
- 2017-09-15 EP EP17768765.4A patent/EP3512959B1/en active Active
- 2017-09-15 AU AU2017328617A patent/AU2017328617B2/en active Active
- 2017-09-15 KR KR1020197007698A patent/KR102468174B1/ko active Active
- 2017-09-15 CN CN201780056666.0A patent/CN109689884A/zh active Pending
-
2021
- 2021-05-07 US US17/314,470 patent/US11345958B2/en active Active
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