KR102468633B1 - 멀티플렉스 실시간 pcr 수행 방법 - Google Patents

멀티플렉스 실시간 pcr 수행 방법 Download PDF

Info

Publication number
KR102468633B1
KR102468633B1 KR1020197007697A KR20197007697A KR102468633B1 KR 102468633 B1 KR102468633 B1 KR 102468633B1 KR 1020197007697 A KR1020197007697 A KR 1020197007697A KR 20197007697 A KR20197007697 A KR 20197007697A KR 102468633 B1 KR102468633 B1 KR 102468633B1
Authority
KR
South Korea
Prior art keywords
nucleic acid
oligonucleotide
moiety
target nucleic
tag portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020197007697A
Other languages
English (en)
Korean (ko)
Other versions
KR20190046880A (ko
Inventor
이고르 코즐로프
아마르 굽타
랜덜 사이키
앨리슨 찬
Original Assignee
에프. 호프만-라 로슈 아게
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=59901522&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR102468633(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 에프. 호프만-라 로슈 아게 filed Critical 에프. 호프만-라 로슈 아게
Publication of KR20190046880A publication Critical patent/KR20190046880A/ko
Application granted granted Critical
Publication of KR102468633B1 publication Critical patent/KR102468633B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/113Real time assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/101Interaction between at least two labels
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020197007697A 2016-09-15 2017-09-15 멀티플렉스 실시간 pcr 수행 방법 Active KR102468633B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662395325P 2016-09-15 2016-09-15
US62/395,325 2016-09-15
US201662435595P 2016-12-16 2016-12-16
US62/435,595 2016-12-16
US201762536871P 2017-07-25 2017-07-25
US62/536,871 2017-07-25
PCT/EP2017/073293 WO2018050828A1 (en) 2016-09-15 2017-09-15 Methods for performing multiplexed real-time pcr

Publications (2)

Publication Number Publication Date
KR20190046880A KR20190046880A (ko) 2019-05-07
KR102468633B1 true KR102468633B1 (ko) 2022-11-18

Family

ID=59901522

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020197007698A Active KR102468174B1 (ko) 2016-09-15 2017-09-15 멀티플렉스 pcr 수행 방법
KR1020197007697A Active KR102468633B1 (ko) 2016-09-15 2017-09-15 멀티플렉스 실시간 pcr 수행 방법

Family Applications Before (1)

Application Number Title Priority Date Filing Date
KR1020197007698A Active KR102468174B1 (ko) 2016-09-15 2017-09-15 멀티플렉스 pcr 수행 방법

Country Status (10)

Country Link
US (3) US11028433B2 (OSRAM)
EP (2) EP3512959B1 (OSRAM)
JP (2) JP7063886B2 (OSRAM)
KR (2) KR102468174B1 (OSRAM)
CN (2) CN109689889A (OSRAM)
AU (2) AU2017328613B2 (OSRAM)
CA (2) CA3033250C (OSRAM)
DK (1) DK3512959T3 (OSRAM)
ES (2) ES2893411T3 (OSRAM)
WO (2) WO2018050824A1 (OSRAM)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7063886B2 (ja) 2016-09-15 2022-05-09 エフ.ホフマン-ラ ロシュ アーゲー マルチプレックスリアルタイムpcrを実施する方法
RU2665631C2 (ru) * 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
CA3088250A1 (en) 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
EP3781708B1 (en) 2018-04-20 2025-01-15 Seegene, Inc. Method for detecting a plurality of target nucleic acid sequences in sample
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
WO2020031156A1 (en) 2018-08-09 2020-02-13 Speedx Pty Ltd Multiplex detection of nucleic acids
US11371080B2 (en) 2018-11-30 2022-06-28 Luminex Corporation Methods and probes for performing PCR with melt analysis for increased multiplexing
WO2020127620A1 (en) * 2018-12-20 2020-06-25 F. Hoffmann-La Roche Ag Detection of target nucleic acid by solid-phase molography
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US20230050863A1 (en) 2019-12-09 2023-02-16 Roche Molecular Systems, Inc. Dicationic fluorescent dyes
CN111534518B (zh) * 2020-05-18 2021-07-23 纳昂达(南京)生物科技有限公司 通用封闭序列及其应用
AU2020256848A1 (en) * 2020-06-30 2022-10-27 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters
CN112195221B (zh) * 2020-09-13 2021-08-06 武汉纳诺德医疗科技有限公司 一种多温度单荧光通道多重实时荧光定量pcr仪及其检测方法
EP4592401A3 (en) * 2020-09-16 2025-10-29 Bruker Spatial Biology, Inc. Chemical compositions and methods of using the same
JP2024500169A (ja) 2020-12-22 2024-01-04 エフ. ホフマン-ラ ロシュ アーゲー 大ストークスシフト蛍光色素を使用して多重化リアルタイムpcrを行うための方法
JP2024522640A (ja) 2021-06-25 2024-06-21 エフ. ホフマン-ラ ロシュ アーゲー 高感度で温度多重化pcrを行うための方法
EP4409027B1 (en) 2021-09-30 2025-11-19 Gen-Probe Incorporated Temperature-selectable fret cassette signaling
US20250361399A1 (en) 2022-06-28 2025-11-27 Roche Molecular Systems, Inc. Fluorescent dyes with large stokes shift
EP4621068A1 (en) * 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
AR132405A1 (es) * 2023-04-19 2025-06-25 Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet Métodos y sistemas para seleccionar biomarcadores basados en metilación del adn para la detección de cáncer colorrectal
AU2024220029A1 (en) * 2023-05-15 2024-11-28 Speedx Pty Ltd Target detection using temperature controlled probes
WO2025053620A1 (ko) * 2023-09-08 2025-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses
EP4606908A1 (en) 2024-02-21 2025-08-27 Roche Diagnostics GmbH Method of increasing the number of usable channels in multiplex pcr

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007013915A1 (en) 2005-07-20 2007-02-01 Bayer Healthcare Llc Gated amperometry
US20110071038A1 (en) 2006-10-20 2011-03-24 Clondiag Gmbh Assay devices and methods for the detection of analytes
WO2016101959A1 (en) * 2014-12-22 2016-06-30 Anapa Biotech A/S Dual quenching assay for multiplex detection of target nucleic acids

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4250257A (en) * 1978-08-24 1981-02-10 Technicon Instruments Corporation Whole blood analyses in porous media
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5352600A (en) 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0550687B1 (en) 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5843691A (en) * 1993-05-15 1998-12-01 Lifescan, Inc. Visually-readable reagent test strip
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5571673A (en) 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
JP2000502882A (ja) 1995-09-08 2000-03-14 ライフ・テクノロジーズ・インコーポレイテッド サーモトガ由来のクローン化dnaポリメラーゼ類およびそれらの変異体
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6427156B1 (en) 1997-01-21 2002-07-30 Xilinx, Inc. Configurable logic block with AND gate for efficient multiplication in FPGAS
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US20050053939A1 (en) 2001-11-09 2005-03-10 Ahmed Chenna Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US8133701B2 (en) 2006-12-05 2012-03-13 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
WO2008083261A1 (en) 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
GB0909333D0 (en) * 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
KR101073875B1 (ko) * 2008-11-28 2011-10-14 한국생명공학연구원 대장암 진단 마커 및 이를 이용한 대장암 진단방법
US8385895B2 (en) 2008-12-18 2013-02-26 Verizon Patent And Licensing Inc. Updating an operating parameter
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
KR101177320B1 (ko) * 2010-02-26 2012-09-10 문우철 Y형 프로브 및 이것의 변형형, 및 이를 이용한 dna 마이크로어레이, 키트 및 유전자 분석방법
US8361720B2 (en) * 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR20130101952A (ko) * 2012-02-02 2013-09-16 주식회사 씨젠 Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
US10106842B2 (en) 2013-03-15 2018-10-23 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
US9828629B2 (en) * 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
WO2014205083A1 (en) 2013-06-19 2014-12-24 Luminex Corporation Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
WO2016025452A1 (en) * 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays
WO2016093619A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures and reference values
CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
JP7063886B2 (ja) 2016-09-15 2022-05-09 エフ.ホフマン-ラ ロシュ アーゲー マルチプレックスリアルタイムpcrを実施する方法
US10590469B2 (en) * 2016-09-15 2020-03-17 Roche Molecular Svstems, Inc. Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007013915A1 (en) 2005-07-20 2007-02-01 Bayer Healthcare Llc Gated amperometry
US20110071038A1 (en) 2006-10-20 2011-03-24 Clondiag Gmbh Assay devices and methods for the detection of analytes
WO2016101959A1 (en) * 2014-12-22 2016-06-30 Anapa Biotech A/S Dual quenching assay for multiplex detection of target nucleic acids

Also Published As

Publication number Publication date
EP3512960A1 (en) 2019-07-24
CA3036119C (en) 2022-09-20
CA3033250C (en) 2023-08-15
KR20190046881A (ko) 2019-05-07
ES2933176T3 (es) 2023-02-02
EP3512960B1 (en) 2022-10-19
ES2893411T3 (es) 2022-02-09
JP7063886B2 (ja) 2022-05-09
EP3512959B1 (en) 2021-08-11
US20210269857A1 (en) 2021-09-02
AU2017328617B2 (en) 2023-06-29
AU2017328617A1 (en) 2019-02-28
CA3036119A1 (en) 2018-03-22
CN109689884A (zh) 2019-04-26
EP3512959A1 (en) 2019-07-24
US11345958B2 (en) 2022-05-31
AU2017328613B2 (en) 2023-05-18
JP7221199B2 (ja) 2023-02-20
CA3033250A1 (en) 2018-03-22
KR102468174B1 (ko) 2022-11-17
US20180073064A1 (en) 2018-03-15
US11028433B2 (en) 2021-06-08
AU2017328613A1 (en) 2019-03-07
CN109689889A (zh) 2019-04-26
JP2019532628A (ja) 2019-11-14
US20180073056A1 (en) 2018-03-15
DK3512959T3 (da) 2021-10-18
WO2018050828A1 (en) 2018-03-22
WO2018050824A1 (en) 2018-03-22
KR20190046880A (ko) 2019-05-07
US11034997B2 (en) 2021-06-15
JP2019528726A (ja) 2019-10-17

Similar Documents

Publication Publication Date Title
KR102468633B1 (ko) 멀티플렉스 실시간 pcr 수행 방법
US11198903B2 (en) Methods for performing multiplexed real-time PCR
EP2129802B1 (en) Methods for detecting nucleic acid sequences
EP3682030B1 (en) Improved polynucleotide sequence detection method
JP6144623B2 (ja) 核酸測定用の核酸プローブ
AU2022294745B2 (en) Detection of multiple target nucleic acid using multiple detection temperatures
JP2024522640A (ja) 高感度で温度多重化pcrを行うための方法
EP3899035B1 (en) Detection of target nucleic acid by solid-phase molography
US20240287584A1 (en) Methods for performing temperature multiplexed pcr with increased sensitivity
EP4621068A1 (en) Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20190315

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20200909

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20220426

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20221026

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20221115

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20221115

End annual number: 3

Start annual number: 1

PG1601 Publication of registration