JP2019518465A5 - - Google Patents
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- JP2019518465A5 JP2019518465A5 JP2018566957A JP2018566957A JP2019518465A5 JP 2019518465 A5 JP2019518465 A5 JP 2019518465A5 JP 2018566957 A JP2018566957 A JP 2018566957A JP 2018566957 A JP2018566957 A JP 2018566957A JP 2019518465 A5 JP2019518465 A5 JP 2019518465A5
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- cells
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- 238000000034 method Methods 0.000 claims description 118
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 claims description 47
- 238000012258 culturing Methods 0.000 claims description 38
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- 239000008103 glucose Substances 0.000 claims description 30
- 239000003112 inhibitor Substances 0.000 claims description 21
- 230000003914 insulin secretion Effects 0.000 claims description 20
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims description 10
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims description 10
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims description 10
- 101000971533 Homo sapiens Killer cell lectin-like receptor subfamily G member 1 Proteins 0.000 claims description 10
- 101000979205 Homo sapiens Transcription factor MafA Proteins 0.000 claims description 10
- 101000939387 Homo sapiens Urocortin-3 Proteins 0.000 claims description 10
- 102100021457 Killer cell lectin-like receptor subfamily G member 1 Human genes 0.000 claims description 10
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims description 10
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims description 10
- 102100029794 Urocortin-3 Human genes 0.000 claims description 10
- 230000001419 dependent effect Effects 0.000 claims description 10
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 10
- OGQSCIYDJSNCMY-UHFFFAOYSA-H iron(3+);methyl-dioxido-oxo-$l^{5}-arsane Chemical compound [Fe+3].[Fe+3].C[As]([O-])([O-])=O.C[As]([O-])([O-])=O.C[As]([O-])([O-])=O OGQSCIYDJSNCMY-UHFFFAOYSA-H 0.000 claims description 10
- 230000006540 mitochondrial respiration Effects 0.000 claims description 9
- 230000000638 stimulation Effects 0.000 claims description 9
- 230000004044 response Effects 0.000 claims description 7
- 108010065691 Biphasic Insulins Proteins 0.000 claims description 6
- 102000058063 Glucose Transporter Type 1 Human genes 0.000 claims description 6
- 108091006296 SLC2A1 Proteins 0.000 claims description 6
- 239000000189 biphasic insulin Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 102000004877 Insulin Human genes 0.000 claims description 5
- 108090001061 Insulin Proteins 0.000 claims description 5
- 229940125396 insulin Drugs 0.000 claims description 5
- 210000002237 B-cell of pancreatic islet Anatomy 0.000 claims description 2
- OMKHWTRUYNAGFG-IEBDPFPHSA-N 3-deazaneplanocin a Chemical compound C1=NC=2C(N)=NC=CC=2N1[C@@H]1C=C(CO)[C@@H](O)[C@H]1O OMKHWTRUYNAGFG-IEBDPFPHSA-N 0.000 claims 3
- NMUSYJAQQFHJEW-UHFFFAOYSA-N 5-Azacytidine Natural products O=C1N=C(N)N=CN1C1C(O)C(O)C(CO)O1 NMUSYJAQQFHJEW-UHFFFAOYSA-N 0.000 claims 3
- NMUSYJAQQFHJEW-KVTDHHQDSA-N 5-azacytidine Chemical compound O=C1N=C(N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 NMUSYJAQQFHJEW-KVTDHHQDSA-N 0.000 claims 3
- 229960002756 azacitidine Drugs 0.000 claims 3
- 238000004114 suspension culture Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 89
- 239000002609 medium Substances 0.000 description 46
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 20
- 210000001778 pluripotent stem cell Anatomy 0.000 description 14
- -1 UCN0646 Chemical compound 0.000 description 13
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 10
- OGNYUTNQZVRGMN-UHFFFAOYSA-N ZM447439 Chemical compound N1=CN=C2C=C(OCCCN3CCOCC3)C(OC)=CC2=C1NC(C=C1)=CC=C1NC(=O)C1=CC=CC=C1 OGNYUTNQZVRGMN-UHFFFAOYSA-N 0.000 description 10
- 229960004308 acetylcysteine Drugs 0.000 description 10
- 229960005070 ascorbic acid Drugs 0.000 description 10
- 235000010323 ascorbic acid Nutrition 0.000 description 10
- 239000011668 ascorbic acid Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 9
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 8
- 229960002897 heparin Drugs 0.000 description 8
- 229920000669 heparin Polymers 0.000 description 8
- 210000004153 islets of langerhan Anatomy 0.000 description 8
- 210000004039 endoderm cell Anatomy 0.000 description 7
- 238000009472 formulation Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- FOORCIAZMIWALX-ULJHMMPZSA-N (z)-n-(4-benzylpiperazin-1-yl)-1-(3,5-dimethyl-1-phenylpyrazol-4-yl)methanimine Chemical compound CC1=NN(C=2C=CC=CC=2)C(C)=C1\C=N/N(CC1)CCN1CC1=CC=CC=C1 FOORCIAZMIWALX-ULJHMMPZSA-N 0.000 description 6
- 102100028071 Fibroblast growth factor 7 Human genes 0.000 description 6
- 101001060261 Homo sapiens Fibroblast growth factor 7 Proteins 0.000 description 6
- 239000012190 activator Substances 0.000 description 6
- 230000036284 oxygen consumption Effects 0.000 description 6
- 230000009996 pancreatic endocrine effect Effects 0.000 description 6
- 210000001900 endoderm Anatomy 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- RNAMYOYQYRYFQY-UHFFFAOYSA-N 2-(4,4-difluoropiperidin-1-yl)-6-methoxy-n-(1-propan-2-ylpiperidin-4-yl)-7-(3-pyrrolidin-1-ylpropoxy)quinazolin-4-amine Chemical compound N1=C(N2CCC(F)(F)CC2)N=C2C=C(OCCCN3CCCC3)C(OC)=CC2=C1NC1CCN(C(C)C)CC1 RNAMYOYQYRYFQY-UHFFFAOYSA-N 0.000 description 4
- SHRCVZJKZJGIHQ-UHFFFAOYSA-N 2-chloro-n-[4-[4-[(2-chloroacetyl)amino]phenyl]sulfonylphenyl]acetamide Chemical compound C1=CC(NC(=O)CCl)=CC=C1S(=O)(=O)C1=CC=C(NC(=O)CCl)C=C1 SHRCVZJKZJGIHQ-UHFFFAOYSA-N 0.000 description 4
- QOECJCJVIMVJGX-UHFFFAOYSA-N 2-cyclohexyl-6-methoxy-N-(1-propan-2-yl-4-piperidinyl)-7-[3-(1-pyrrolidinyl)propoxy]-4-quinazolinamine Chemical compound N1=C(C2CCCCC2)N=C2C=C(OCCCN3CCCC3)C(OC)=CC2=C1NC1CCN(C(C)C)CC1 QOECJCJVIMVJGX-UHFFFAOYSA-N 0.000 description 4
- TXZPMHLMPKIUGK-UHFFFAOYSA-N 2-methoxy-N-(3-methyl-2-oxo-1,4-dihydroquinazolin-6-yl)benzenesulfonamide Chemical compound COC1=CC=CC=C1S(=O)(=O)NC1=CC=C(NC(=O)N(C)C2)C2=C1 TXZPMHLMPKIUGK-UHFFFAOYSA-N 0.000 description 4
- PTJGLFIIZFVFJV-UHFFFAOYSA-N N'-hydroxy-N-(3-pyridinyl)octanediamide Chemical compound ONC(=O)CCCCCCC(=O)NC1=CC=CN=C1 PTJGLFIIZFVFJV-UHFFFAOYSA-N 0.000 description 4
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 4
- 229930002330 retinoic acid Natural products 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 229960001727 tretinoin Drugs 0.000 description 4
- 210000003890 endocrine cell Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 210000002438 upper gastrointestinal tract Anatomy 0.000 description 3
- 229940125373 Gamma-Secretase Inhibitor Drugs 0.000 description 2
- 102000044880 Wnt3A Human genes 0.000 description 2
- 108700013515 Wnt3A Proteins 0.000 description 2
- 108010023082 activin A Proteins 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000003540 gamma secretase inhibitor Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 101150068520 wnt3a gene Proteins 0.000 description 2
- ADDJDJQFPKUMAF-SFHVURJKSA-N (4s)-5-amino-5-oxo-4-[[4-(4-phenylthiophen-2-yl)benzoyl]amino]pentanoic acid Chemical compound C1=CC(C(=O)N[C@@H](CCC(O)=O)C(=O)N)=CC=C1C1=CC(C=2C=CC=CC=2)=CS1 ADDJDJQFPKUMAF-SFHVURJKSA-N 0.000 description 1
- 102100039939 Growth/differentiation factor 8 Human genes 0.000 description 1
- 108010056852 Myostatin Proteins 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021196949A JP7429680B2 (ja) | 2016-06-21 | 2021-12-03 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2023173813A JP2024012312A (ja) | 2016-06-21 | 2023-10-05 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2025072068A JP2025121937A (ja) | 2016-06-21 | 2025-04-24 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662352968P | 2016-06-21 | 2016-06-21 | |
| US62/352,968 | 2016-06-21 | ||
| PCT/US2017/037373 WO2017222879A1 (en) | 2016-06-21 | 2017-06-14 | Generation of human pluripotent stem cell derived functional beta cells showing a glucose-dependent mitochondrial respiration and two-phase insulin secretion response |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021196949A Division JP7429680B2 (ja) | 2016-06-21 | 2021-12-03 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019518465A JP2019518465A (ja) | 2019-07-04 |
| JP2019518465A5 true JP2019518465A5 (https=) | 2020-07-27 |
| JP7044726B2 JP7044726B2 (ja) | 2022-03-30 |
Family
ID=60659269
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018566957A Active JP7044726B2 (ja) | 2016-06-21 | 2017-06-14 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2021196949A Active JP7429680B2 (ja) | 2016-06-21 | 2021-12-03 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2023173813A Pending JP2024012312A (ja) | 2016-06-21 | 2023-10-05 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2025072068A Pending JP2025121937A (ja) | 2016-06-21 | 2025-04-24 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021196949A Active JP7429680B2 (ja) | 2016-06-21 | 2021-12-03 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2023173813A Pending JP2024012312A (ja) | 2016-06-21 | 2023-10-05 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
| JP2025072068A Pending JP2025121937A (ja) | 2016-06-21 | 2025-04-24 | グルコース依存性ミトコンドリア呼吸及び二相インスリン分泌応答を示すヒト多能性幹細胞由来の機能的ベータ細胞の生成 |
Country Status (24)
| Country | Link |
|---|---|
| US (3) | US10633635B2 (https=) |
| EP (3) | EP3472303B1 (https=) |
| JP (4) | JP7044726B2 (https=) |
| KR (5) | KR102489946B1 (https=) |
| CN (2) | CN116286663A (https=) |
| AR (1) | AR108847A1 (https=) |
| AU (3) | AU2017282638C1 (https=) |
| CA (1) | CA3027879A1 (https=) |
| DK (2) | DK3472303T3 (https=) |
| ES (2) | ES2939233T3 (https=) |
| FI (1) | FI4194548T3 (https=) |
| HR (1) | HRP20260022T1 (https=) |
| LT (1) | LT4194548T (https=) |
| MA (2) | MA71715B1 (https=) |
| MD (1) | MD4194548T2 (https=) |
| PL (1) | PL4194548T3 (https=) |
| PT (1) | PT4194548T (https=) |
| RS (1) | RS67611B1 (https=) |
| RU (1) | RU2752499C2 (https=) |
| SG (1) | SG11201810550WA (https=) |
| SI (1) | SI4194548T1 (https=) |
| SM (1) | SMT202600006T1 (https=) |
| TW (1) | TW201809266A (https=) |
| WO (1) | WO2017222879A1 (https=) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111565707A (zh) | 2017-06-14 | 2020-08-21 | 森玛治疗公司 | 用于递送治疗物的装置和方法 |
| KR102738995B1 (ko) * | 2017-09-11 | 2024-12-06 | 노보 노르디스크 에이/에스 | 시험관 내 줄기 세포로부터 유래된 nkx6.1 및 c-펩티드 공발현 세포의 농축 |
| EP3710021A4 (en) | 2017-11-15 | 2021-08-11 | Semma Therapeutics, Inc. | ISLAND CELL MANUFACTURING COMPOSITIONS AND METHOD OF USE |
| EP3758719A4 (en) * | 2018-03-02 | 2021-12-08 | Vertex Pharmaceuticals Inc. | METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS |
| WO2019208788A1 (ja) * | 2018-04-27 | 2019-10-31 | 株式会社カネカ | 膵臓β細胞の製造方法 |
| JP2021522819A (ja) * | 2018-05-07 | 2021-09-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | インビトロベータ細胞分化に関連する細胞集団および遺伝子発現 |
| US12421499B2 (en) | 2018-05-11 | 2025-09-23 | Cornell University | Method of differentiation of human pluripotent stem cells to monohormonal cells |
| WO2020033879A1 (en) * | 2018-08-10 | 2020-02-13 | Semma Therapeutics, Inc. | Stem cell derived islet differentiation |
| US12378572B2 (en) | 2018-09-07 | 2025-08-05 | Crispr Therapeutics Ag | Universal donor cells |
| CA3139292C (en) | 2019-05-31 | 2025-06-10 | Viacyte, Inc. | CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES |
| CN114173837A (zh) | 2019-05-31 | 2022-03-11 | W.L.戈尔及同仁股份有限公司 | 生物相容性膜复合材料 |
| EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
| EP3976236A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates Inc. | A biocompatible membrane composite |
| CA3144948A1 (en) * | 2019-06-25 | 2020-12-30 | Vertex Pharmaceuticals Incorporated | Enhanced differentiation of beta cells |
| CN114390924B (zh) * | 2019-06-27 | 2024-08-16 | 达纳-法伯癌症研究公司 | 用于蛋白质降解的化合物、组合物和方法 |
| PH12022550542A1 (en) | 2019-09-05 | 2023-03-20 | Crispr Therapeutics Ag | Universal donor cells |
| WO2021102305A1 (en) * | 2019-11-20 | 2021-05-27 | Washington University | Methods and compositions for generating functionally mature beta cells and uses thereof |
| WO2021233116A1 (zh) * | 2020-05-21 | 2021-11-25 | 北京大学 | 多能干细胞的成分明确、无异源的培养系统 |
| WO2022026932A2 (en) | 2020-07-31 | 2022-02-03 | Vertex Pharmaceuticals Incorporated | Differentiation of pancreatic endocrine cells |
| WO2022144855A1 (en) | 2020-12-31 | 2022-07-07 | Crispr Therapeutics Ag | Universal donor cells |
| CN114958726B (zh) * | 2021-02-23 | 2025-03-21 | 海门雨霖细胞科技有限责任公司 | 化学诱导成纤维细胞重编程为肺干细胞的化学小分子组合物及其应用 |
| WO2022204377A1 (en) * | 2021-03-24 | 2022-09-29 | Salk Institute For Biological Studies | NOVEL CHEMICAL COMBINATIONS AND METHODS OF USE THEREOF, TOWARDS DIFFERENTIATION OF HUMAN PROGENITOR CELLS INTO FUNCTIONAL β CELLS |
| WO2022236173A1 (en) * | 2021-05-07 | 2022-11-10 | Mayo Foundation For Medical Education And Research | Treating liver disease |
| US20220403340A1 (en) * | 2021-06-11 | 2022-12-22 | Washington University | Media for cryopreserved cells and methods of making and using same |
| CA3232971A1 (en) | 2021-11-01 | 2023-05-04 | George Harb | Stem cell derived pancreatic islet differentiation |
| CN114317401B (zh) * | 2021-12-07 | 2022-10-04 | 浙江大学 | 一种促进胰岛前体细胞长期稳定传代的方法 |
| KR102848180B1 (ko) * | 2021-12-15 | 2025-08-19 | 한국화학연구원 | 오로라 키나아제 억제제를 이용하는 심근세포 및 심장 오가노이드의 제조방법 |
| US20240034996A1 (en) * | 2022-07-29 | 2024-02-01 | Therapeutic Solutions International, Inc. | Gene modified ipsc derived cellular compositions for regeneration and immune modulation |
| WO2025117331A1 (en) | 2023-12-01 | 2025-06-05 | Eli Lilly And Company | Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same |
| WO2025119299A1 (en) * | 2023-12-08 | 2025-06-12 | Hangzhou Reprogenix Bioscience, Inc. | Method for differentiating pancreatic beta cells |
| WO2025202874A1 (en) | 2024-03-25 | 2025-10-02 | Crispr Therapeutics Ag | Genetically modified cells expressing glp-1 receptor agonist |
| WO2026069163A1 (en) * | 2024-09-25 | 2026-04-02 | Crispr Therapeutics Ag | Differentiation method for producing immature beta cells |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| WO2005001080A2 (en) | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Postpartum-derived cells for use in treatment of disease of the heart and circulatory system |
| CN103898047B (zh) | 2003-12-23 | 2020-03-03 | 维亚希特公司 | 定形内胚层 |
| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| US8586357B2 (en) * | 2003-12-23 | 2013-11-19 | Viacyte, Inc. | Markers of definitive endoderm |
| CN103356706B (zh) | 2005-03-31 | 2016-01-20 | 斯丹姆涅恩有限公司 | 促进无疤愈合或改善皮肤外观的美容制剂 |
| AU2006202209B2 (en) | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| US7807459B2 (en) | 2005-09-27 | 2010-10-05 | Reneuron, Inc. | EphA4-positive human adult pancreatic endocrine progenitor cells |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| US20090269313A1 (en) * | 2006-07-19 | 2009-10-29 | Diakine Therapeutics, Inc. | Encapsulation system |
| AU2007277364B2 (en) | 2006-07-26 | 2010-08-12 | Viacyte, Inc. | Methods of producing pancreatic hormones |
| CA2954431C (en) | 2007-11-27 | 2021-08-24 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic cells |
| US8338170B2 (en) | 2008-04-21 | 2012-12-25 | Viacyte, Inc. | Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells |
| US8728812B2 (en) | 2008-04-22 | 2014-05-20 | President And Fellows Of Harvard College | Compositions and methods for promoting the generation of PDX1+ pancreatic cells |
| EP2942392B1 (en) | 2008-06-30 | 2018-10-03 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
| AU2009267167A1 (en) | 2008-06-30 | 2010-01-07 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
| US9234178B2 (en) * | 2008-10-31 | 2016-01-12 | Janssen Biotech, Inc. | Differentiation of human pluripotent stem cells |
| US8008075B2 (en) | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| US8895300B2 (en) | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
| PL2494035T3 (pl) | 2009-10-29 | 2018-07-31 | Janssen Biotech, Inc. | Pluripotencjalne komórki macierzyste |
| SG10201408552YA (en) | 2009-12-23 | 2015-02-27 | Janssen Biotech Inc | Differentiation of human embryonic stem cells |
| KR101877077B1 (ko) | 2010-08-09 | 2018-07-10 | 다케다 야쿠힌 고교 가부시키가이샤 | 췌호르몬-생성 세포의 제조 방법 |
| FI2794857T3 (fi) | 2011-12-22 | 2026-03-25 | Janssen Biotech Inc | Ihmisen alkion kantasolujen erilaistaminen yksittäisiksi hormonaalisiksi insuliinipositiivisiksi soluiksi |
| EP3450544A1 (en) * | 2012-06-08 | 2019-03-06 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endodrine cells |
| CN104903440B (zh) | 2012-09-03 | 2018-04-06 | 诺和诺德股份有限公司 | 使用小分子从多能干细胞产生胰内胚层 |
| US9902938B2 (en) * | 2012-12-26 | 2018-02-27 | Kyoto University | Method for inducing exocrine pancreatic cells |
| US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
| ES2837763T3 (es) | 2012-12-31 | 2021-07-01 | Janssen Biotech Inc | Cultivo de células madre embrionarias humanas en la interconexión aire-líquido para la diferenciación en células endocrinas pancreáticas |
| BR112015015714A2 (pt) | 2012-12-31 | 2017-07-11 | Janssen Biotech Inc | suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas |
| DK2938723T3 (da) | 2012-12-31 | 2023-02-20 | Janssen Biotech Inc | Differentiering af humane embryonale stamceller til pancreatiske endokrine celler under anvendelse af hb9-regulatorer |
| US20140329704A1 (en) * | 2013-03-28 | 2014-11-06 | President And Fellows Of Harvard College | Markers for mature beta-cells and methods of using the same |
| CN120796168A (zh) * | 2013-06-11 | 2025-10-17 | 哈佛学院校长同事会 | SC-β细胞以及用于产生其的组合物和方法 |
| WO2015175307A1 (en) | 2014-05-16 | 2015-11-19 | Janssen Biotech, Inc. | Use of small molecules to enhance mafa expression in pancreatic endocrine cells |
| US11021687B2 (en) * | 2016-01-08 | 2021-06-01 | The Brigham And Women's Hospital, Inc. | Production of differentiated enteroendocrine cells and insulin producing cells |
| CN108699515A (zh) * | 2016-02-24 | 2018-10-23 | 诺和诺德股份有限公司 | 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 |
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