JP2015516161A5 - - Google Patents
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- Publication number
- JP2015516161A5 JP2015516161A5 JP2015511622A JP2015511622A JP2015516161A5 JP 2015516161 A5 JP2015516161 A5 JP 2015516161A5 JP 2015511622 A JP2015511622 A JP 2015511622A JP 2015511622 A JP2015511622 A JP 2015511622A JP 2015516161 A5 JP2015516161 A5 JP 2015516161A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- marker
- indicator
- expressing
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims description 76
- 210000001900 endoderm Anatomy 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 29
- 238000010899 nucleation Methods 0.000 claims description 21
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 21
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 7
- 108010082117 matrigel Proteins 0.000 claims description 4
- 210000000130 stem cell Anatomy 0.000 claims description 3
- 239000003550 marker Substances 0.000 claims 37
- 230000004069 differentiation Effects 0.000 claims 2
- 210000003372 endocrine gland Anatomy 0.000 claims 2
- 210000004039 endoderm cell Anatomy 0.000 claims 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
- 230000009996 pancreatic endocrine effect Effects 0.000 claims 2
- 108010083123 CDX2 Transcription Factor Proteins 0.000 claims 1
- 101100518002 Danio rerio nkx2.2a gene Proteins 0.000 claims 1
- 108700014808 Homeobox Protein Nkx-2.2 Proteins 0.000 claims 1
- 102100031671 Homeobox protein CDX-2 Human genes 0.000 claims 1
- 102100027886 Homeobox protein Nkx-2.2 Human genes 0.000 claims 1
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims 1
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims 1
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims 1
- 101100460496 Homo sapiens NKX2-2 gene Proteins 0.000 claims 1
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims 1
- 101000976653 Homo sapiens Zinc finger protein ZIC 1 Proteins 0.000 claims 1
- 102000004877 Insulin Human genes 0.000 claims 1
- 108090001061 Insulin Proteins 0.000 claims 1
- 102100032063 Neurogenic differentiation factor 1 Human genes 0.000 claims 1
- 108050000588 Neurogenic differentiation factor 1 Proteins 0.000 claims 1
- 102100038553 Neurogenin-3 Human genes 0.000 claims 1
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims 1
- 101710144033 Pancreas/duodenum homeobox protein 1 Proteins 0.000 claims 1
- 102100023497 Zinc finger protein ZIC 1 Human genes 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 210000001654 germ layer Anatomy 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 229940125396 insulin Drugs 0.000 claims 1
- 230000003827 upregulation Effects 0.000 claims 1
- 239000002609 medium Substances 0.000 description 6
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 102100039939 Growth/differentiation factor 8 Human genes 0.000 description 2
- 101000886562 Homo sapiens Growth/differentiation factor 8 Proteins 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 description 1
- 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 description 1
- 241000288906 Primates Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 238000002454 metastable transfer emission spectrometry Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- CPUDPFPXCZDNGI-UHFFFAOYSA-N triethoxy(methyl)silane Chemical compound CCO[Si](C)(OCC)OCC CPUDPFPXCZDNGI-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261643684P | 2012-05-07 | 2012-05-07 | |
| US61/643,684 | 2012-05-07 | ||
| PCT/US2013/039940 WO2013169769A1 (en) | 2012-05-07 | 2013-05-07 | Differentiation of human embryonic stem cells into pancreatic endoderm |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015516161A JP2015516161A (ja) | 2015-06-11 |
| JP2015516161A5 true JP2015516161A5 (https=) | 2017-11-09 |
| JP6450674B2 JP6450674B2 (ja) | 2019-01-09 |
Family
ID=49551213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015511622A Expired - Fee Related JP6450674B2 (ja) | 2012-05-07 | 2013-05-07 | ヒト胚性幹細胞の膵臓の内胚葉への分化 |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20140162359A1 (https=) |
| EP (1) | EP2847319A4 (https=) |
| JP (1) | JP6450674B2 (https=) |
| KR (1) | KR20150014478A (https=) |
| CN (1) | CN104284977A (https=) |
| AR (1) | AR090970A1 (https=) |
| AU (1) | AU2013259706A1 (https=) |
| BR (1) | BR112014027783A2 (https=) |
| CA (1) | CA2872770A1 (https=) |
| HK (1) | HK1207885A1 (https=) |
| IL (1) | IL235132A0 (https=) |
| IN (1) | IN2014DN08561A (https=) |
| MX (1) | MX2014013524A (https=) |
| PH (1) | PH12014502686A1 (https=) |
| RU (1) | RU2668814C2 (https=) |
| SG (1) | SG11201406884SA (https=) |
| WO (1) | WO2013169769A1 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120796168A (zh) | 2013-06-11 | 2025-10-17 | 哈佛学院校长同事会 | SC-β细胞以及用于产生其的组合物和方法 |
| CN107614678B (zh) | 2014-12-18 | 2021-04-30 | 哈佛学院校长同事会 | 干细胞来源的β细胞的产生方法及其使用方法 |
| WO2016100898A1 (en) | 2014-12-18 | 2016-06-23 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof |
| EP3234110B1 (en) | 2014-12-18 | 2024-02-28 | President and Fellows of Harvard College | METHODS FOR GENERATING STEM CELL-DERIVED ß CELLS AND USES THEREOF |
| CN106467918B (zh) * | 2015-08-18 | 2020-07-31 | 中国科学技术大学先进技术研究院 | 一种基于人皮肤细胞的胰岛素分泌细胞的诱导方法及应用 |
| CN111094547B (zh) * | 2017-06-14 | 2024-02-09 | 德国亥姆霍兹慕尼黑中心健康与环境研究中心（有限公司） | 用于纯化源自人胚胎干细胞的内胚层和胰腺内胚层细胞的方法 |
| EP3710021A4 (en) | 2017-11-15 | 2021-08-11 | Semma Therapeutics, Inc. | ISLAND CELL MANUFACTURING COMPOSITIONS AND METHOD OF USE |
| WO2020033879A1 (en) | 2018-08-10 | 2020-02-13 | Semma Therapeutics, Inc. | Stem cell derived islet differentiation |
| US12378572B2 (en) | 2018-09-07 | 2025-08-05 | Crispr Therapeutics Ag | Universal donor cells |
| CA3139292C (en) | 2019-05-31 | 2025-06-10 | Viacyte, Inc. | CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES |
| EP3976236A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates Inc. | A biocompatible membrane composite |
| CN114173837A (zh) | 2019-05-31 | 2022-03-11 | W.L.戈尔及同仁股份有限公司 | 生物相容性膜复合材料 |
| EP3975926A1 (en) | 2019-05-31 | 2022-04-06 | W.L. Gore & Associates, Inc. | A biocompatible membrane composite |
| CA3150235A1 (en) | 2019-09-05 | 2021-03-11 | Alireza Rezania | Universal donor cells |
| PH12022550542A1 (en) | 2019-09-05 | 2023-03-20 | Crispr Therapeutics Ag | Universal donor cells |
| WO2022144855A1 (en) | 2020-12-31 | 2022-07-07 | Crispr Therapeutics Ag | Universal donor cells |
| CN113046306B (zh) * | 2021-03-12 | 2022-10-18 | 广东东阳光药业有限公司 | 一种多能干细胞的培养方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| IL162131A0 (en) | 2001-12-07 | 2005-11-20 | Geron Corp | Islet cells from human embryonic stem cells |
| CN103898047B (zh) | 2003-12-23 | 2020-03-03 | 维亚希特公司 | 定形内胚层 |
| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| US8741643B2 (en) * | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| AU2007277364B2 (en) | 2006-07-26 | 2010-08-12 | Viacyte, Inc. | Methods of producing pancreatic hormones |
| EP2559756B1 (en) * | 2007-07-01 | 2019-12-18 | Janssen Biotech, Inc. | Single pluripotent stem cell culture |
| EP3957716A1 (en) * | 2007-07-18 | 2022-02-23 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| DK2185693T3 (da) * | 2007-07-31 | 2019-09-23 | Lifescan Inc | Differentiering af humane embryoniske stamceller |
| US7939322B2 (en) * | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
| EP2942392B1 (en) | 2008-06-30 | 2018-10-03 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
| US20100028307A1 (en) * | 2008-07-31 | 2010-02-04 | O'neil John J | Pluripotent stem cell differentiation |
| US9234178B2 (en) * | 2008-10-31 | 2016-01-12 | Janssen Biotech, Inc. | Differentiation of human pluripotent stem cells |
| CN103952372B (zh) * | 2009-07-20 | 2016-10-05 | 詹森生物科技公司 | 人胚胎干细胞的分化 |
| US9018010B2 (en) * | 2009-11-12 | 2015-04-28 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| SG10201501513TA (en) * | 2010-03-02 | 2015-04-29 | Univ Singapore | Culture additives to boost stem cell proliferation and differentiation response |
| WO2011118211A1 (ja) * | 2010-03-23 | 2011-09-29 | 株式会社クラレ | 多能性哺乳細胞を分化させる培養方法 |
| CN103154237B (zh) * | 2010-08-31 | 2016-03-16 | 詹森生物科技公司 | 多能干细胞的分化 |
-
2013
- 2013-05-07 BR BR112014027783A patent/BR112014027783A2/pt not_active Application Discontinuation
- 2013-05-07 CA CA2872770A patent/CA2872770A1/en not_active Abandoned
- 2013-05-07 CN CN201380024226.9A patent/CN104284977A/zh active Pending
- 2013-05-07 EP EP13786955.8A patent/EP2847319A4/en not_active Withdrawn
- 2013-05-07 HK HK15108511.6A patent/HK1207885A1/xx unknown
- 2013-05-07 SG SG11201406884SA patent/SG11201406884SA/en unknown
- 2013-05-07 AU AU2013259706A patent/AU2013259706A1/en not_active Abandoned
- 2013-05-07 WO PCT/US2013/039940 patent/WO2013169769A1/en not_active Ceased
- 2013-05-07 AR ARP130101563A patent/AR090970A1/es unknown
- 2013-05-07 MX MX2014013524A patent/MX2014013524A/es unknown
- 2013-05-07 JP JP2015511622A patent/JP6450674B2/ja not_active Expired - Fee Related
- 2013-05-07 US US13/888,968 patent/US20140162359A1/en not_active Abandoned
- 2013-05-07 RU RU2014149185A patent/RU2668814C2/ru not_active IP Right Cessation
- 2013-05-07 KR KR1020147033897A patent/KR20150014478A/ko not_active Ceased
-
2014
- 2014-10-14 IN IN8561DEN2014 patent/IN2014DN08561A/en unknown
- 2014-10-19 IL IL235132A patent/IL235132A0/en unknown
- 2014-12-02 PH PH12014502686A patent/PH12014502686A1/en unknown
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