JP2019517799A5 - - Google Patents

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JP2019517799A5
JP2019517799A5 JP2018564828A JP2018564828A JP2019517799A5 JP 2019517799 A5 JP2019517799 A5 JP 2019517799A5 JP 2018564828 A JP2018564828 A JP 2018564828A JP 2018564828 A JP2018564828 A JP 2018564828A JP 2019517799 A5 JP2019517799 A5 JP 2019517799A5
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Japan
Prior art keywords
variant
polymerase
functional fragment
dna polymerase
seq
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JP2018564828A
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English (en)
Japanese (ja)
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JP7118510B2 (ja
JP2019517799A (ja
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Priority claimed from FR1655475A external-priority patent/FR3052462A1/fr
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Priority to JP2022105854A priority Critical patent/JP2022137127A/ja
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JP2018564828A 2016-06-14 2017-06-13 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント Active JP7118510B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022105854A JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1655475 2016-06-14
FR1655475A FR3052462A1 (fr) 2016-06-14 2016-06-14 Variants d'une adn polymerase de la famille polx
PCT/FR2017/051519 WO2017216472A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx

Related Child Applications (1)

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JP2022105854A Division JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント

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JP2019517799A JP2019517799A (ja) 2019-06-27
JP2019517799A5 true JP2019517799A5 (enExample) 2020-07-27
JP7118510B2 JP7118510B2 (ja) 2022-08-16

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JP2018564828A Active JP7118510B2 (ja) 2016-06-14 2017-06-13 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント
JP2022105854A Pending JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント

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Country Status (11)

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US (3) US20200002690A1 (enExample)
EP (1) EP3469075A2 (enExample)
JP (2) JP7118510B2 (enExample)
KR (1) KR102522263B1 (enExample)
CN (1) CN109477080A (enExample)
AU (2) AU2017286477C1 (enExample)
CA (1) CA3024184A1 (enExample)
FR (2) FR3052462A1 (enExample)
IL (1) IL263503B2 (enExample)
SG (2) SG11201809961TA (enExample)
WO (1) WO2017216472A2 (enExample)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10774316B2 (en) * 2014-10-20 2020-09-15 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
AU2018272302A1 (en) 2017-05-26 2019-12-19 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
EP3665274B1 (en) 2017-08-07 2025-10-15 DNA Script Variants of family a dna polymerase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2019135007A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
JP2022513031A (ja) * 2018-11-14 2022-02-07 ディーエヌエー スクリプト 末端デオキシヌクレオチジルトランスフェラーゼバリアントおよびその使用
CN113423841A (zh) 2018-12-13 2021-09-21 Dna斯克瑞普特公司 细胞和生物分子上的直接寡核苷酸合成
JP7491934B2 (ja) 2019-01-03 2024-05-28 ディーエヌエー スクリプト オリゴヌクレオチドセットのワンポット合成
GB201901501D0 (en) 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
EP3924500A1 (en) 2019-02-12 2021-12-22 DNA Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
EP4407026A3 (en) * 2019-05-28 2025-01-15 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
EP3744854A1 (en) * 2019-05-28 2020-12-02 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
AU2020319829A1 (en) 2019-07-30 2022-02-24 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(A) and poly(U) polymerases
JP2022543568A (ja) 2019-08-01 2022-10-13 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なしの酵素による合成における長い配列の収量の増加
CN110331136B (zh) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
JP2022547918A (ja) 2019-09-09 2022-11-16 ディーエヌエー スクリプト 光開裂性結合を用いる鋳型なし酵素的ポリヌクレオチド合成
US20220403436A1 (en) 2019-09-23 2022-12-22 Dna Script Increasing Long-Sequence Yields in Template-Free Enzymatic Synthesis of Polynucleotides
CA3157418A1 (en) 2019-11-13 2021-05-20 Dna Script High efficiency template-free enzymatic synthesis of polynucleotides
IL293820A (en) * 2019-12-12 2022-08-01 Dna Script Chimeric-terminal deoxynucleotidyltransferases for template-independent enzymatic synthesis of polynucleotides
WO2021122539A1 (en) 2019-12-16 2021-06-24 Dna Script Template-free enzymatic polynucleotide synthesis using dismutationless terminal deoxynucleotidyl transferase variants
CN115151652B (zh) 2020-02-25 2025-02-07 Dna斯克瑞普特公司 用于酶促合成多核苷酸的方法和设备
EP4139446A1 (en) 2020-04-20 2023-03-01 DNA Script Terminal deoxynucleotidyl transferase variants and uses thereof
WO2021231483A1 (en) 2020-05-12 2021-11-18 Illumina, Inc. Thermostable terminal deoxynucleotidyl transferase
EP4150065A2 (en) 2020-05-12 2023-03-22 Illumina Inc Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
EP4162035A4 (en) * 2020-06-03 2025-03-12 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
US20230235372A1 (en) 2020-06-12 2023-07-27 Synhelix Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes
KR20230024399A (ko) 2020-06-16 2023-02-20 디엔에이 스크립트 효소적 폴리뉴클레오티드 합성을 위한 시스템, 장치 및 키트
EP4182472A1 (en) 2020-07-15 2023-05-24 DNA Script Massively parallel enzymatic synthesis of polynucleotides
WO2022019836A1 (en) 2020-07-21 2022-01-27 Illumina Singapore Pte Ltd Base-modified nucleotides as substrates for tdt-based enzymatic nucleic acid
EP4192947A1 (en) * 2020-08-04 2023-06-14 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (tdt) enzymes
GB2598152B (en) * 2020-08-21 2025-04-16 Nuclera Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
CA3193386A1 (en) 2020-09-22 2022-03-31 Mikhael SOSKINE Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
US20240052391A1 (en) 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
US12391970B2 (en) 2020-11-11 2025-08-19 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
CN112322715B (zh) * 2020-11-17 2022-11-25 清华大学 一种核酸测序方法
CN117222744A (zh) 2021-04-02 2023-12-12 Dna斯克瑞普特公司 用于酶法合成g4-易发的多核苷酸的方法和试剂盒
NL2032097B1 (en) 2021-06-10 2024-03-29 Dna Script Enzymatic synthesis of polynucleotides using 3'-o-amino-2'-deoxyribonucleoside triphosphate monomers
WO2023025488A1 (en) 2021-08-23 2023-03-02 Dna Script Method for generating double-stranded nucleic acid
EP4395933A1 (en) 2021-09-03 2024-07-10 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
US12565641B2 (en) 2021-10-15 2026-03-03 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
US20250002879A1 (en) 2021-11-10 2025-01-02 Dna Script NOVEL TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TdT) VARIANTS
WO2023083997A2 (en) 2021-11-10 2023-05-19 Dna Script Novel terminal deoxynucleotidyl
CN116555216A (zh) * 2022-01-28 2023-08-08 中国科学院天津工业生物技术研究所 可控合成单链dna的末端转移酶变体及应用
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
US20250205700A1 (en) 2022-03-11 2025-06-26 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023240202A2 (en) * 2022-06-08 2023-12-14 Ansa Biotechnologies, Inc. Modified terminal deoxynucleotidyl transferase polymerases
EP4555083A1 (en) 2022-07-13 2025-05-21 DNA Script Method of purifying solution comprising polynucleotide
EP4630540A1 (en) 2022-12-05 2025-10-15 DNA Script Variants of poly(a) polymerase and uses thereof
EP4623082A1 (en) 2022-12-09 2025-10-01 DNA Script In vitro enzymatical rna synthesis
WO2024141628A1 (en) 2022-12-31 2024-07-04 Dna Script Variable viscosity inks for inkjet delivery of enzyme reagents
WO2024153642A1 (en) 2023-01-16 2024-07-25 Dna Script Scarless template-free enzymatic synthesis of polynucleotides
EP4652292A1 (en) 2023-01-16 2025-11-26 DNA Script Inkjet-assisted enzymatic nucleic acid synthesis
CN120530202A (zh) 2023-01-26 2025-08-22 Dna斯克瑞普特公司 多核苷酸探针的酶促合成
CN121039286A (zh) 2023-05-03 2025-11-28 Dna斯克瑞普特公司 用于3'-o-氨基多核苷酸的去保护的试剂
WO2024241324A1 (en) * 2023-05-24 2024-11-28 Scintium Ltd Method for training an artificial intelligence (ai) model and generating a 3d computer-aided design (cad) and system therefor
WO2024256604A1 (en) 2023-06-15 2024-12-19 Dna Script Salt addition during enzymatic polynucleotide synthesis
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
CN121532522A (zh) 2023-07-27 2026-02-13 Dna斯克瑞普特公司 用于产生多个多核苷酸的方法
WO2025088024A1 (en) 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
WO2025093659A1 (en) 2023-11-04 2025-05-08 Dna Script 3'-o-aminooxymethyl blocked nucleotides and use thereof in enzymatic synthesis of polynucleotides
WO2025137872A1 (zh) * 2023-12-26 2025-07-03 华大工程生物学长荡湖研究所 一种末端脱氧核糖核苷转移酶突变体及其制备方法和应用
WO2025155239A1 (en) * 2024-01-17 2025-07-24 Agency For Science, Technology And Research X family dna polymerases and uses thereof
EP4653525A1 (en) * 2024-05-21 2025-11-26 Uab "Biomatter Designs" Engineered terminal deoxynucleotidyl transferase polymerases
WO2026008717A2 (en) 2024-07-02 2026-01-08 Dna Script Method for enzymatic synthesis of polynucleotides
WO2026008711A2 (en) 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis
WO2026017834A1 (en) 2024-07-19 2026-01-22 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis and cleavage

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004689A (en) 1982-02-22 1991-04-02 Biogen, Massachusetts DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields
IL71691A (en) 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
BG52073B2 (en) 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
ES2571446T3 (es) * 2008-11-03 2016-05-25 Kapa Biosystems Inc ADN polimerasas quiméricas
EP2741784B1 (en) * 2011-03-02 2017-05-17 Board Of Regents, The University Of Texas System Tusc2 therapies
EP3080158A4 (en) * 2013-12-09 2018-03-21 Adimab, LLC Polyclonal mixtures of antibodies, and methods of making and using them
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
WO2016064880A1 (en) * 2014-10-20 2016-04-28 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis

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