JP2019517799A5 - - Google Patents

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JP2019517799A5
JP2019517799A5 JP2018564828A JP2018564828A JP2019517799A5 JP 2019517799 A5 JP2019517799 A5 JP 2019517799A5 JP 2018564828 A JP2018564828 A JP 2018564828A JP 2018564828 A JP2018564828 A JP 2018564828A JP 2019517799 A5 JP2019517799 A5 JP 2019517799A5
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Japan
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variant
polymerase
functional fragment
dna polymerase
seq
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JP2018564828A
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English (en)
Japanese (ja)
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JP7118510B2 (ja
JP2019517799A (ja
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Priority claimed from FR1655475A external-priority patent/FR3052462A1/fr
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Priority to JP2022105854A priority Critical patent/JP2022137127A/ja
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JP2018564828A 2016-06-14 2017-06-13 polXファミリーDNAポリメラーゼのバリアント Active JP7118510B2 (ja)

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Application Number Priority Date Filing Date Title
JP2022105854A JP2022137127A (ja) 2016-06-14 2022-06-30 polXファミリーDNAポリメラーゼのバリアント

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1655475A FR3052462A1 (fr) 2016-06-14 2016-06-14 Variants d'une adn polymerase de la famille polx
FR1655475 2016-06-14
PCT/FR2017/051519 WO2017216472A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx

Related Child Applications (1)

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JP2022105854A Division JP2022137127A (ja) 2016-06-14 2022-06-30 polXファミリーDNAポリメラーゼのバリアント

Publications (3)

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JP2019517799A JP2019517799A (ja) 2019-06-27
JP2019517799A5 true JP2019517799A5 (enExample) 2020-07-27
JP7118510B2 JP7118510B2 (ja) 2022-08-16

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JP2018564828A Active JP7118510B2 (ja) 2016-06-14 2017-06-13 polXファミリーDNAポリメラーゼのバリアント
JP2022105854A Pending JP2022137127A (ja) 2016-06-14 2022-06-30 polXファミリーDNAポリメラーゼのバリアント

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US (3) US20200002690A1 (enExample)
EP (1) EP3469075A2 (enExample)
JP (2) JP7118510B2 (enExample)
KR (1) KR102522263B1 (enExample)
CN (1) CN109477080A (enExample)
AU (2) AU2017286477C1 (enExample)
CA (1) CA3024184A1 (enExample)
FR (2) FR3052462A1 (enExample)
IL (1) IL263503B2 (enExample)
SG (2) SG10202009688QA (enExample)
WO (1) WO2017216472A2 (enExample)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10774316B2 (en) * 2014-10-20 2020-09-15 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
WO2018215803A1 (en) 2017-05-26 2018-11-29 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
EP3665274B1 (en) 2017-08-07 2025-10-15 DNA Script Variants of family a dna polymerase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2019135007A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US12421628B2 (en) 2018-07-23 2025-09-23 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
WO2020099451A1 (en) 2018-11-14 2020-05-22 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
KR20210104779A (ko) 2018-12-13 2021-08-25 디엔에이 스크립트 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성
CN113272442B (zh) 2019-01-03 2024-03-15 Dna斯克瑞普特公司 寡核苷酸的集合的一锅合成
GB201901501D0 (en) 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
KR20210125496A (ko) * 2019-02-12 2021-10-18 디엔에이 스크립트 폴리뉴클레오티드의 주형-유리 효소 합성에서 효율적 생성물 절단
ES2982551T3 (es) * 2019-05-28 2024-10-16 Dna Script Variantes de desoxinucleotidil transferasa terminal y usos de las mismas
EP3744854A1 (en) * 2019-05-28 2020-12-02 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
EP4004222A1 (en) 2019-07-30 2022-06-01 DNA Script Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
US20220403435A1 (en) 2019-08-01 2022-12-22 Dna Script Sas Increasing Long-Sequence Yields In Template-Free Enzymatic Synthesis of Polynucleotides
CN110331136B (zh) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
EP4028537A1 (en) 2019-09-09 2022-07-20 DNA Script Template-free enzymatic polynucleotide synthesis using photocleavable linkages
JP2022549196A (ja) 2019-09-23 2022-11-24 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加
JP2023501651A (ja) 2019-11-13 2023-01-18 ディーエヌエー スクリプト ポリヌクレオチドの高効率テンプレートなしの酵素的合成
EP4073240A1 (en) * 2019-12-12 2022-10-19 DNA Script Chimeric terminal deoxynucleotidyl transferases for template-free enzymatic synthesis of polynucleotides
WO2021122539A1 (en) 2019-12-16 2021-06-24 Dna Script Template-free enzymatic polynucleotide synthesis using dismutationless terminal deoxynucleotidyl transferase variants
US20230089448A1 (en) 2020-02-25 2023-03-23 Dna Script Method And Apparatus for Enzymatic Synthesis of Polynucleotides
KR20230002825A (ko) 2020-04-20 2023-01-05 디엔에이 스크립트 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도
US12110516B2 (en) 2020-05-12 2024-10-08 Illumina, Inc. Thermostable terminal deoxynucleotidyl transferase
CN115916967A (zh) 2020-05-12 2023-04-04 伊鲁米纳公司 使用重组末端脱氧核苷酸转移酶生成具有经修饰的碱基的核酸
KR20240016237A (ko) * 2020-06-03 2024-02-06 몰레큘러 어셈블리즈, 인크. 폴리데옥시뉴클레오티드 합성을 위한 변형된 템플레이트-비의존성 효소
KR20230078992A (ko) 2020-06-12 2023-06-05 신헬릭스 열안정성 효소를 사용한 핵산의 초기 주형 독립적 합성
EP4164784B1 (en) 2020-06-16 2025-06-25 DNA Script Method for enzymatic polynucleotide synthesis
EP4182472A1 (en) 2020-07-15 2023-05-24 DNA Script Massively parallel enzymatic synthesis of polynucleotides
KR20230041975A (ko) 2020-07-21 2023-03-27 일루미나, 싱가포르 피티이 엘티디 Tdt-기반 효소적 핵산을 위한 기질로서의 염기-변형된 뉴클레오티드
EP4192947A1 (en) * 2020-08-04 2023-06-14 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (tdt) enzymes
GB2598152B (en) * 2020-08-21 2025-04-16 Nuclera Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
WO2022063835A1 (en) 2020-09-22 2022-03-31 Dna Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
US20240052391A1 (en) 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
US12391970B2 (en) 2020-11-11 2025-08-19 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
CN112322715B (zh) * 2020-11-17 2022-11-25 清华大学 一种核酸测序方法
DE102022107811A1 (de) 2021-04-02 2022-10-06 Dna Script Methoden und kits für die enzymatische synthese von zu-g4-neigenden polynukleotiden
DE102022114351A1 (de) 2021-06-10 2022-12-15 Dna Script Enzymatische synthese von polynukleotiden mittels 3'-o-amino-2'-deoxyribonukleosid triphosphat monomeren
EP4392554A1 (en) 2021-08-23 2024-07-03 DNA Script Method for generating double-stranded nucleic acid
US20240299945A1 (en) 2021-09-03 2024-09-12 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
CA3236657A1 (en) 2021-11-10 2023-05-19 Daniel MCHALE Novel terminal deoxynucleotidyl transferase (tdt) variant and uses thereof
WO2023083999A2 (en) 2021-11-10 2023-05-19 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants
CN116555216A (zh) * 2022-01-28 2023-08-08 中国科学院天津工业生物技术研究所 可控合成单链dna的末端转移酶变体及应用
WO2023170266A1 (en) 2022-03-11 2023-09-14 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023240202A2 (en) * 2022-06-08 2023-12-14 Ansa Biotechnologies, Inc. Modified terminal deoxynucleotidyl transferase polymerases
EP4555083A1 (en) 2022-07-13 2025-05-21 DNA Script Method of purifying solution comprising polynucleotide
EP4630540A1 (en) 2022-12-05 2025-10-15 DNA Script Variants of poly(a) polymerase and uses thereof
CN120359296A (zh) 2022-12-09 2025-07-22 Dna斯克瑞普特公司 体外酶促rna合成
CN120603956A (zh) 2022-12-31 2025-09-05 Dna斯克瑞普特公司 用于酶试剂的喷墨递送的可变粘度油墨
WO2024153643A1 (en) 2023-01-16 2024-07-25 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis
WO2024153642A1 (en) 2023-01-16 2024-07-25 Dna Script Scarless template-free enzymatic synthesis of polynucleotides
EP4655411A2 (en) 2023-01-26 2025-12-03 DNA Script Enzymatic synthesis of polynucleotide probes
CN121039286A (zh) 2023-05-03 2025-11-28 Dna斯克瑞普特公司 用于3'-o-氨基多核苷酸的去保护的试剂
WO2024241324A1 (en) * 2023-05-24 2024-11-28 Scintium Ltd Method for training an artificial intelligence (ai) model and generating a 3d computer-aided design (cad) and system therefor
WO2024256604A1 (en) 2023-06-15 2024-12-19 Dna Script Salt addition during enzymatic polynucleotide synthesis
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
WO2025021984A1 (en) 2023-07-27 2025-01-30 Dna Script Method for generating a plurality of polynucleotides
WO2025088024A1 (en) 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
WO2025093659A1 (en) 2023-11-04 2025-05-08 Dna Script 3'-o-aminooxymethyl blocked nucleotides and use thereof in enzymatic synthesis of polynucleotides
WO2025137872A1 (zh) * 2023-12-26 2025-07-03 华大工程生物学长荡湖研究所 一种末端脱氧核糖核苷转移酶突变体及其制备方法和应用
WO2025155239A1 (en) * 2024-01-17 2025-07-24 Agency For Science, Technology And Research X family dna polymerases and uses thereof
EP4653525A1 (en) * 2024-05-21 2025-11-26 Uab "Biomatter Designs" Engineered terminal deoxynucleotidyl transferase polymerases

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004689A (en) 1982-02-22 1991-04-02 Biogen, Massachusetts DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields
IL71691A (en) 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
BG52073B2 (en) 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
US9023633B2 (en) * 2008-11-03 2015-05-05 Kapa Biosystems Chimeric DNA polymerases
CA2864394C (en) * 2011-03-02 2021-10-19 Jack Roth A method of predicting a response to a tusc2 therapy
US10684289B2 (en) * 2013-12-09 2020-06-16 Adimab, Llc Polyclonal mixtures of antibodies, and methods of making and using them
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3699283A1 (en) * 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis

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