FR3052462A1 - Variants d'une adn polymerase de la famille polx - Google Patents
Variants d'une adn polymerase de la famille polx Download PDFInfo
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- FR3052462A1 FR3052462A1 FR1655475A FR1655475A FR3052462A1 FR 3052462 A1 FR3052462 A1 FR 3052462A1 FR 1655475 A FR1655475 A FR 1655475A FR 1655475 A FR1655475 A FR 1655475A FR 3052462 A1 FR3052462 A1 FR 3052462A1
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- Prior art keywords
- variant
- residue
- dna polymerase
- nucleic acid
- polx family
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- 230000002378 acidificating effect Effects 0.000 description 1
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- 150000001412 amines Chemical class 0.000 description 1
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- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
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- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- YSMODUONRAFBET-UHFFFAOYSA-N delta-DL-hydroxylysine Natural products NCC(O)CCC(N)C(O)=O YSMODUONRAFBET-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 101150007302 dntt gene Proteins 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- YSMODUONRAFBET-UHNVWZDZSA-N erythro-5-hydroxy-L-lysine Chemical compound NC[C@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-UHNVWZDZSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- QJHBJHUKURJDLG-UHFFFAOYSA-N hydroxy-L-lysine Natural products NCCCCC(NO)C(O)=O QJHBJHUKURJDLG-UHFFFAOYSA-N 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
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- 239000002243 precursor Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
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- 238000002708 random mutagenesis Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
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- YSMODUONRAFBET-WHFBIAKZSA-N threo-5-hydroxy-L-lysine Chemical compound NC[C@@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-WHFBIAKZSA-N 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
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- 241000701161 unidentified adenovirus Species 0.000 description 1
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- 239000004474 valine Substances 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (17)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1655475A FR3052462A1 (fr) | 2016-06-14 | 2016-06-14 | Variants d'une adn polymerase de la famille polx |
| CN201780037427.0A CN109477080A (zh) | 2016-06-14 | 2017-06-13 | polX家族的DNA聚合酶的变体 |
| US16/309,414 US20200002690A1 (en) | 2016-06-14 | 2017-06-13 | Variants of a DNA Polymerase of the Polx Family |
| SG11201809961TA SG11201809961TA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
| AU2017286477A AU2017286477C1 (en) | 2016-06-14 | 2017-06-13 | Variants of a DNA polymerase of the polX family |
| IL263503A IL263503B2 (en) | 2016-06-14 | 2017-06-13 | DNA polymerase variants from the polX family |
| SG10202009688QA SG10202009688QA (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
| PCT/FR2017/051519 WO2017216472A2 (fr) | 2016-06-14 | 2017-06-13 | Variants d'une adn polymérase de la famille polx |
| EP17746156.3A EP3469075A2 (fr) | 2016-06-14 | 2017-06-13 | Variants d'une adn polymérase de la famille polx |
| CA3024184A CA3024184A1 (en) | 2016-06-14 | 2017-06-13 | Variants of a dna polymerase of the polx family |
| KR1020187034921A KR102522263B1 (ko) | 2016-06-14 | 2017-06-13 | polX 패밀리의 DNA 폴리머라제의 변형체 |
| JP2018564828A JP7118510B2 (ja) | 2016-06-14 | 2017-06-13 | ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント |
| FR1756408A FR3053699B1 (fr) | 2016-06-14 | 2017-07-07 | Variants d'une adn polymerase de la famille polx |
| JP2022105854A JP2022137127A (ja) | 2016-06-14 | 2022-06-30 | ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント |
| US17/815,490 US20230167421A1 (en) | 2016-06-14 | 2022-07-27 | Variants of a DNA Polymerase of the Polx Family |
| US18/516,616 US20240124855A1 (en) | 2016-06-14 | 2023-11-21 | Variants of a dna polymerase of the polx family |
| AU2023282219A AU2023282219A1 (en) | 2016-06-14 | 2023-12-13 | Variants of a DNA polymerase of the polX family |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1655475A FR3052462A1 (fr) | 2016-06-14 | 2016-06-14 | Variants d'une adn polymerase de la famille polx |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FR3052462A1 true FR3052462A1 (fr) | 2017-12-15 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR1655475A Pending FR3052462A1 (fr) | 2016-06-14 | 2016-06-14 | Variants d'une adn polymerase de la famille polx |
| FR1756408A Active FR3053699B1 (fr) | 2016-06-14 | 2017-07-07 | Variants d'une adn polymerase de la famille polx |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR1756408A Active FR3053699B1 (fr) | 2016-06-14 | 2017-07-07 | Variants d'une adn polymerase de la famille polx |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20200002690A1 (enExample) |
| EP (1) | EP3469075A2 (enExample) |
| JP (2) | JP7118510B2 (enExample) |
| KR (1) | KR102522263B1 (enExample) |
| CN (1) | CN109477080A (enExample) |
| AU (2) | AU2017286477C1 (enExample) |
| CA (1) | CA3024184A1 (enExample) |
| FR (2) | FR3052462A1 (enExample) |
| IL (1) | IL263503B2 (enExample) |
| SG (2) | SG11201809961TA (enExample) |
| WO (1) | WO2017216472A2 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220002687A1 (en) * | 2018-11-14 | 2022-01-06 | Dna Script | Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof |
| WO2022029427A1 (en) * | 2020-08-04 | 2022-02-10 | Nuclera Nucleics Ltd | MODIFIED TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TdT) ENZYMES |
| CN114207140A (zh) * | 2019-05-28 | 2022-03-18 | Dna斯克瑞普特公司 | 末端脱氧核苷酸转移酶变体及其用途 |
| US11993800B2 (en) * | 2017-05-26 | 2024-05-28 | Nuclera Ltd. | Use of terminal transferase enzyme in nucleic acid synthesis |
Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10774316B2 (en) * | 2014-10-20 | 2020-09-15 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| WO2019030149A1 (en) | 2017-08-07 | 2019-02-14 | Dna Script | VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF |
| WO2019135007A1 (en) * | 2018-01-08 | 2019-07-11 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| JP7430701B2 (ja) | 2018-07-23 | 2024-02-13 | ディーエヌエー スクリプト | 核酸鎖の大規模並列酵素合成 |
| SG11202105441WA (en) | 2018-12-13 | 2021-06-29 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| US12428668B2 (en) | 2019-01-03 | 2025-09-30 | Dna Script | One pot synthesis of sets of oligonucleotides |
| WO2020146425A1 (en) | 2019-01-07 | 2020-07-16 | Elegen Corporation | Methods of using microfluidic positional encoding devices |
| GB201901501D0 (en) * | 2019-02-04 | 2019-03-27 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| WO2020165137A1 (en) | 2019-02-12 | 2020-08-20 | Dna Script | Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. |
| EP3744854A1 (en) * | 2019-05-28 | 2020-12-02 | DNA Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| WO2021018919A1 (en) | 2019-07-30 | 2021-02-04 | Dna Script | Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases |
| CN114430778B (zh) | 2019-08-01 | 2024-07-05 | Dna斯克瑞普特公司 | 增加多核苷酸的无模板酶促合成中长序列的产量 |
| CN110331136B (zh) * | 2019-09-05 | 2019-12-24 | 中国科学院天津工业生物技术研究所 | 一种末端脱氧核糖核苷转移酶变体及其应用 |
| US20220315970A1 (en) | 2019-09-09 | 2022-10-06 | Dna Script | Template-Free Enzymatic Polynucleotide Synthesis Using Photocleavable Linkages |
| JP2022549196A (ja) | 2019-09-23 | 2022-11-24 | ディーエヌエー スクリプト | ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加 |
| US20220411840A1 (en) | 2019-11-13 | 2022-12-29 | Dna Script | High Efficiency Template-Free Enzymatic Synthesis of Polynucleotides |
| US20230062303A1 (en) | 2019-12-12 | 2023-03-02 | Dna Script | Chimeric Terminal Deoxynucleotidyl Transferases For Template-Free Enzymatic Synthesis Of Polynucleotides |
| EP4077703A1 (en) | 2019-12-16 | 2022-10-26 | DNA Script | Template-free enzymatic polynucleotide synthesis using dismutationless terminal deoxynucleotidyl transferase variants |
| EP4110940B1 (en) | 2020-02-25 | 2024-03-27 | DNA Script | Method and apparatus for enzymatic synthesis of polynucleotides |
| KR20230002825A (ko) | 2020-04-20 | 2023-01-05 | 디엔에이 스크립트 | 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도 |
| US12209266B2 (en) | 2020-05-12 | 2025-01-28 | Illumina, Inc. | Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase |
| JP7828081B2 (ja) * | 2020-05-12 | 2026-03-11 | ナショナル ユニヴァーシティー オブ シンガポール | 熱安定性末端デオキシヌクレオチジルトランスフェラーゼ |
| KR20240016237A (ko) * | 2020-06-03 | 2024-02-06 | 몰레큘러 어셈블리즈, 인크. | 폴리데옥시뉴클레오티드 합성을 위한 변형된 템플레이트-비의존성 효소 |
| WO2021250265A2 (en) | 2020-06-12 | 2021-12-16 | Synhelix | Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes |
| ES3070893T3 (en) | 2020-06-16 | 2026-06-18 | Dna Script | Method for enzymatic polynucleotide synthesis |
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| US12371680B2 (en) | 2018-11-14 | 2025-07-29 | Dna Script Sas | Terminal deoxynucleotidyl transferase variants and uses thereof |
| CN114207140A (zh) * | 2019-05-28 | 2022-03-18 | Dna斯克瑞普特公司 | 末端脱氧核苷酸转移酶变体及其用途 |
| WO2022029427A1 (en) * | 2020-08-04 | 2022-02-10 | Nuclera Nucleics Ltd | MODIFIED TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TdT) ENZYMES |
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| FR3053699A1 (fr) | 2018-01-12 |
| AU2017286477C1 (en) | 2023-12-21 |
| AU2023282219A1 (en) | 2024-01-18 |
| EP3469075A2 (fr) | 2019-04-17 |
| WO2017216472A3 (fr) | 2018-03-15 |
| US20230167421A1 (en) | 2023-06-01 |
| IL263503A (en) | 2019-02-03 |
| US20240124855A1 (en) | 2024-04-18 |
| WO2017216472A2 (fr) | 2017-12-21 |
| SG11201809961TA (en) | 2018-12-28 |
| IL263503B1 (en) | 2024-12-01 |
| CN109477080A (zh) | 2019-03-15 |
| JP7118510B2 (ja) | 2022-08-16 |
| AU2017286477B2 (en) | 2023-09-14 |
| IL263503B2 (en) | 2025-04-01 |
| SG10202009688QA (en) | 2020-11-27 |
| KR20190024883A (ko) | 2019-03-08 |
| KR102522263B1 (ko) | 2023-04-18 |
| FR3053699B1 (fr) | 2021-04-02 |
| JP2022137127A (ja) | 2022-09-21 |
| CA3024184A1 (en) | 2017-12-21 |
| JP2019517799A (ja) | 2019-06-27 |
| AU2017286477A1 (en) | 2018-12-06 |
| US20200002690A1 (en) | 2020-01-02 |
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