IL263503B2 - DNA polymerase variants from the polX family - Google Patents
DNA polymerase variants from the polX familyInfo
- Publication number
- IL263503B2 IL263503B2 IL263503A IL26350318A IL263503B2 IL 263503 B2 IL263503 B2 IL 263503B2 IL 263503 A IL263503 A IL 263503A IL 26350318 A IL26350318 A IL 26350318A IL 263503 B2 IL263503 B2 IL 263503B2
- Authority
- IL
- Israel
- Prior art keywords
- variant
- nucleic acid
- tdt
- variants
- seq
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1655475A FR3052462A1 (fr) | 2016-06-14 | 2016-06-14 | Variants d'une adn polymerase de la famille polx |
| PCT/FR2017/051519 WO2017216472A2 (fr) | 2016-06-14 | 2017-06-13 | Variants d'une adn polymérase de la famille polx |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| IL263503A IL263503A (en) | 2019-02-03 |
| IL263503B1 IL263503B1 (en) | 2024-12-01 |
| IL263503B2 true IL263503B2 (en) | 2025-04-01 |
Family
ID=57348782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL263503A IL263503B2 (en) | 2016-06-14 | 2017-06-13 | DNA polymerase variants from the polX family |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20200002690A1 (enExample) |
| EP (1) | EP3469075A2 (enExample) |
| JP (2) | JP7118510B2 (enExample) |
| KR (1) | KR102522263B1 (enExample) |
| CN (1) | CN109477080A (enExample) |
| AU (2) | AU2017286477C1 (enExample) |
| CA (1) | CA3024184A1 (enExample) |
| FR (2) | FR3052462A1 (enExample) |
| IL (1) | IL263503B2 (enExample) |
| SG (2) | SG10202009688QA (enExample) |
| WO (1) | WO2017216472A2 (enExample) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10774316B2 (en) * | 2014-10-20 | 2020-09-15 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide synthesis |
| WO2018215803A1 (en) | 2017-05-26 | 2018-11-29 | Nuclera Nucleics Ltd | Use of terminal transferase enzyme in nucleic acid synthesis |
| EP3665274B1 (en) | 2017-08-07 | 2025-10-15 | DNA Script | Variants of family a dna polymerase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| WO2019135007A1 (en) * | 2018-01-08 | 2019-07-11 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US12421628B2 (en) | 2018-07-23 | 2025-09-23 | Dna Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| WO2020099451A1 (en) | 2018-11-14 | 2020-05-22 | Dna Script | Terminal deoxynucleotidyl transferase variants and uses thereof |
| KR20210104779A (ko) | 2018-12-13 | 2021-08-25 | 디엔에이 스크립트 | 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성 |
| CN113272442B (zh) | 2019-01-03 | 2024-03-15 | Dna斯克瑞普特公司 | 寡核苷酸的集合的一锅合成 |
| GB201901501D0 (en) | 2019-02-04 | 2019-03-27 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| KR20210125496A (ko) * | 2019-02-12 | 2021-10-18 | 디엔에이 스크립트 | 폴리뉴클레오티드의 주형-유리 효소 합성에서 효율적 생성물 절단 |
| ES2982551T3 (es) * | 2019-05-28 | 2024-10-16 | Dna Script | Variantes de desoxinucleotidil transferasa terminal y usos de las mismas |
| EP3744854A1 (en) * | 2019-05-28 | 2020-12-02 | DNA Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| EP4004222A1 (en) | 2019-07-30 | 2022-06-01 | DNA Script | Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases |
| US20220403435A1 (en) | 2019-08-01 | 2022-12-22 | Dna Script Sas | Increasing Long-Sequence Yields In Template-Free Enzymatic Synthesis of Polynucleotides |
| CN110331136B (zh) * | 2019-09-05 | 2019-12-24 | 中国科学院天津工业生物技术研究所 | 一种末端脱氧核糖核苷转移酶变体及其应用 |
| EP4028537A1 (en) | 2019-09-09 | 2022-07-20 | DNA Script | Template-free enzymatic polynucleotide synthesis using photocleavable linkages |
| JP2022549196A (ja) | 2019-09-23 | 2022-11-24 | ディーエヌエー スクリプト | ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加 |
| JP2023501651A (ja) | 2019-11-13 | 2023-01-18 | ディーエヌエー スクリプト | ポリヌクレオチドの高効率テンプレートなしの酵素的合成 |
| EP4073240A1 (en) * | 2019-12-12 | 2022-10-19 | DNA Script | Chimeric terminal deoxynucleotidyl transferases for template-free enzymatic synthesis of polynucleotides |
| WO2021122539A1 (en) | 2019-12-16 | 2021-06-24 | Dna Script | Template-free enzymatic polynucleotide synthesis using dismutationless terminal deoxynucleotidyl transferase variants |
| US20230089448A1 (en) | 2020-02-25 | 2023-03-23 | Dna Script | Method And Apparatus for Enzymatic Synthesis of Polynucleotides |
| KR20230002825A (ko) | 2020-04-20 | 2023-01-05 | 디엔에이 스크립트 | 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도 |
| US12110516B2 (en) | 2020-05-12 | 2024-10-08 | Illumina, Inc. | Thermostable terminal deoxynucleotidyl transferase |
| CN115916967A (zh) | 2020-05-12 | 2023-04-04 | 伊鲁米纳公司 | 使用重组末端脱氧核苷酸转移酶生成具有经修饰的碱基的核酸 |
| KR20240016237A (ko) * | 2020-06-03 | 2024-02-06 | 몰레큘러 어셈블리즈, 인크. | 폴리데옥시뉴클레오티드 합성을 위한 변형된 템플레이트-비의존성 효소 |
| KR20230078992A (ko) | 2020-06-12 | 2023-06-05 | 신헬릭스 | 열안정성 효소를 사용한 핵산의 초기 주형 독립적 합성 |
| EP4164784B1 (en) | 2020-06-16 | 2025-06-25 | DNA Script | Method for enzymatic polynucleotide synthesis |
| EP4182472A1 (en) | 2020-07-15 | 2023-05-24 | DNA Script | Massively parallel enzymatic synthesis of polynucleotides |
| KR20230041975A (ko) | 2020-07-21 | 2023-03-27 | 일루미나, 싱가포르 피티이 엘티디 | Tdt-기반 효소적 핵산을 위한 기질로서의 염기-변형된 뉴클레오티드 |
| EP4192947A1 (en) * | 2020-08-04 | 2023-06-14 | Nuclera Nucleics Ltd | Modified terminal deoxynucleotidyl transferase (tdt) enzymes |
| GB2598152B (en) * | 2020-08-21 | 2025-04-16 | Nuclera Ltd | Modified terminal deoxynucleotidyl transferase (TdT) enzymes |
| WO2022063835A1 (en) | 2020-09-22 | 2022-03-31 | Dna Script | Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof |
| US20240052391A1 (en) | 2020-10-29 | 2024-02-15 | Dna Script | Enzymatic Synthesis of Polynucleotide Probes |
| US12391970B2 (en) | 2020-11-11 | 2025-08-19 | Microsoft Technology Licensing, Llc | Spatial control of polynucleotide synthesis by strand capping |
| CN112322715B (zh) * | 2020-11-17 | 2022-11-25 | 清华大学 | 一种核酸测序方法 |
| DE102022107811A1 (de) | 2021-04-02 | 2022-10-06 | Dna Script | Methoden und kits für die enzymatische synthese von zu-g4-neigenden polynukleotiden |
| DE102022114351A1 (de) | 2021-06-10 | 2022-12-15 | Dna Script | Enzymatische synthese von polynukleotiden mittels 3'-o-amino-2'-deoxyribonukleosid triphosphat monomeren |
| EP4392554A1 (en) | 2021-08-23 | 2024-07-03 | DNA Script | Method for generating double-stranded nucleic acid |
| US20240299945A1 (en) | 2021-09-03 | 2024-09-12 | Elegen Corporation | Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources |
| CA3236657A1 (en) | 2021-11-10 | 2023-05-19 | Daniel MCHALE | Novel terminal deoxynucleotidyl transferase (tdt) variant and uses thereof |
| WO2023083999A2 (en) | 2021-11-10 | 2023-05-19 | Dna Script | Novel terminal deoxynucleotidyl transferase (tdt) variants |
| CN116555216A (zh) * | 2022-01-28 | 2023-08-08 | 中国科学院天津工业生物技术研究所 | 可控合成单链dna的末端转移酶变体及应用 |
| WO2023170266A1 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Automation station for enzymatic polynucleotide synthesis |
| WO2023170259A1 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Modular accessory rack |
| WO2023170286A2 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Alignment post and secure mechanism for enzymatic polynucleotide synthesis |
| WO2023170258A1 (en) | 2022-03-11 | 2023-09-14 | Dna Script | Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap |
| WO2023240202A2 (en) * | 2022-06-08 | 2023-12-14 | Ansa Biotechnologies, Inc. | Modified terminal deoxynucleotidyl transferase polymerases |
| EP4555083A1 (en) | 2022-07-13 | 2025-05-21 | DNA Script | Method of purifying solution comprising polynucleotide |
| EP4630540A1 (en) | 2022-12-05 | 2025-10-15 | DNA Script | Variants of poly(a) polymerase and uses thereof |
| CN120359296A (zh) | 2022-12-09 | 2025-07-22 | Dna斯克瑞普特公司 | 体外酶促rna合成 |
| CN120603956A (zh) | 2022-12-31 | 2025-09-05 | Dna斯克瑞普特公司 | 用于酶试剂的喷墨递送的可变粘度油墨 |
| WO2024153643A1 (en) | 2023-01-16 | 2024-07-25 | Dna Script | Inkjet-assisted enzymatic nucleic acid synthesis |
| WO2024153642A1 (en) | 2023-01-16 | 2024-07-25 | Dna Script | Scarless template-free enzymatic synthesis of polynucleotides |
| EP4655411A2 (en) | 2023-01-26 | 2025-12-03 | DNA Script | Enzymatic synthesis of polynucleotide probes |
| CN121039286A (zh) | 2023-05-03 | 2025-11-28 | Dna斯克瑞普特公司 | 用于3'-o-氨基多核苷酸的去保护的试剂 |
| WO2024241324A1 (en) * | 2023-05-24 | 2024-11-28 | Scintium Ltd | Method for training an artificial intelligence (ai) model and generating a 3d computer-aided design (cad) and system therefor |
| WO2024256604A1 (en) | 2023-06-15 | 2024-12-19 | Dna Script | Salt addition during enzymatic polynucleotide synthesis |
| WO2025003434A1 (en) | 2023-06-30 | 2025-01-02 | Dna Script | Nucleic acid synthesis on reusable support |
| WO2025021984A1 (en) | 2023-07-27 | 2025-01-30 | Dna Script | Method for generating a plurality of polynucleotides |
| WO2025088024A1 (en) | 2023-10-24 | 2025-05-01 | Dna Script | Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof |
| WO2025093659A1 (en) | 2023-11-04 | 2025-05-08 | Dna Script | 3'-o-aminooxymethyl blocked nucleotides and use thereof in enzymatic synthesis of polynucleotides |
| WO2025137872A1 (zh) * | 2023-12-26 | 2025-07-03 | 华大工程生物学长荡湖研究所 | 一种末端脱氧核糖核苷转移酶突变体及其制备方法和应用 |
| WO2025155239A1 (en) * | 2024-01-17 | 2025-07-24 | Agency For Science, Technology And Research | X family dna polymerases and uses thereof |
| EP4653525A1 (en) * | 2024-05-21 | 2025-11-26 | Uab "Biomatter Designs" | Engineered terminal deoxynucleotidyl transferase polymerases |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016064880A1 (en) * | 2014-10-20 | 2016-04-28 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide systhesis |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5004689A (en) | 1982-02-22 | 1991-04-02 | Biogen, Massachusetts | DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields |
| IL71691A (en) | 1984-04-27 | 1991-04-15 | Yeda Res & Dev | Production of interferon-ypsilon |
| US5047335A (en) | 1988-12-21 | 1991-09-10 | The Regents Of The University Of Calif. | Process for controlling intracellular glycosylation of proteins |
| BG52073B2 (en) | 1990-01-24 | 1996-04-30 | Inst Molekuljarna Biolog | Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine |
| US6812339B1 (en) * | 2000-09-08 | 2004-11-02 | Applera Corporation | Polymorphisms in known genes associated with human disease, methods of detection and uses thereof |
| US9023633B2 (en) * | 2008-11-03 | 2015-05-05 | Kapa Biosystems | Chimeric DNA polymerases |
| CA2864394C (en) * | 2011-03-02 | 2021-10-19 | Jack Roth | A method of predicting a response to a tusc2 therapy |
| US10684289B2 (en) * | 2013-12-09 | 2020-06-16 | Adimab, Llc | Polyclonal mixtures of antibodies, and methods of making and using them |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
-
2016
- 2016-06-14 FR FR1655475A patent/FR3052462A1/fr active Pending
-
2017
- 2017-06-13 KR KR1020187034921A patent/KR102522263B1/ko active Active
- 2017-06-13 SG SG10202009688QA patent/SG10202009688QA/en unknown
- 2017-06-13 IL IL263503A patent/IL263503B2/en unknown
- 2017-06-13 CN CN201780037427.0A patent/CN109477080A/zh active Pending
- 2017-06-13 US US16/309,414 patent/US20200002690A1/en not_active Abandoned
- 2017-06-13 SG SG11201809961TA patent/SG11201809961TA/en unknown
- 2017-06-13 JP JP2018564828A patent/JP7118510B2/ja active Active
- 2017-06-13 EP EP17746156.3A patent/EP3469075A2/fr active Pending
- 2017-06-13 WO PCT/FR2017/051519 patent/WO2017216472A2/fr not_active Ceased
- 2017-06-13 AU AU2017286477A patent/AU2017286477C1/en active Active
- 2017-06-13 CA CA3024184A patent/CA3024184A1/en active Pending
- 2017-07-07 FR FR1756408A patent/FR3053699B1/fr active Active
-
2022
- 2022-06-30 JP JP2022105854A patent/JP2022137127A/ja active Pending
- 2022-07-27 US US17/815,490 patent/US20230167421A1/en active Pending
-
2023
- 2023-11-21 US US18/516,616 patent/US20240124855A1/en active Pending
- 2023-12-13 AU AU2023282219A patent/AU2023282219A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016064880A1 (en) * | 2014-10-20 | 2016-04-28 | Molecular Assemblies, Inc. | Modified template-independent enzymes for polydeoxynucleotide systhesis |
Non-Patent Citations (1)
| Title |
|---|
| YANG, BAOLI, KAREN N. GATHY, AND MARY SUE COLEMAN., T-CELL SPECIFIC AVIAN TDT: CHARACTERIZATION OF THE CDNA AND RECOMBINANT ENZYME., 11 June 1995 (1995-06-11) * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP7118510B2 (ja) | 2022-08-16 |
| JP2019517799A (ja) | 2019-06-27 |
| WO2017216472A3 (fr) | 2018-03-15 |
| WO2017216472A2 (fr) | 2017-12-21 |
| IL263503B1 (en) | 2024-12-01 |
| SG11201809961TA (en) | 2018-12-28 |
| FR3053699A1 (fr) | 2018-01-12 |
| FR3052462A1 (fr) | 2017-12-15 |
| SG10202009688QA (en) | 2020-11-27 |
| EP3469075A2 (fr) | 2019-04-17 |
| JP2022137127A (ja) | 2022-09-21 |
| IL263503A (en) | 2019-02-03 |
| CA3024184A1 (en) | 2017-12-21 |
| CN109477080A (zh) | 2019-03-15 |
| US20230167421A1 (en) | 2023-06-01 |
| AU2023282219A1 (en) | 2024-01-18 |
| KR102522263B1 (ko) | 2023-04-18 |
| US20240124855A1 (en) | 2024-04-18 |
| AU2017286477B2 (en) | 2023-09-14 |
| FR3053699B1 (fr) | 2021-04-02 |
| US20200002690A1 (en) | 2020-01-02 |
| KR20190024883A (ko) | 2019-03-08 |
| AU2017286477C1 (en) | 2023-12-21 |
| AU2017286477A1 (en) | 2018-12-06 |
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