KR102522263B1 - polX 패밀리의 DNA 폴리머라제의 변형체 - Google Patents

polX 패밀리의 DNA 폴리머라제의 변형체 Download PDF

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KR102522263B1
KR102522263B1 KR1020187034921A KR20187034921A KR102522263B1 KR 102522263 B1 KR102522263 B1 KR 102522263B1 KR 1020187034921 A KR1020187034921 A KR 1020187034921A KR 20187034921 A KR20187034921 A KR 20187034921A KR 102522263 B1 KR102522263 B1 KR 102522263B1
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KR20190024883A (ko
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토마스 이버트
마크 델라뤼
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디엔에이 스크립트
앵스티띠 파스퇴르
상뜨로 나쇼날 드 라 러쉐르쉐 샹띠피크
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020187034921A 2016-06-14 2017-06-13 polX 패밀리의 DNA 폴리머라제의 변형체 Active KR102522263B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1655475 2016-06-14
FR1655475A FR3052462A1 (fr) 2016-06-14 2016-06-14 Variants d'une adn polymerase de la famille polx
PCT/FR2017/051519 WO2017216472A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx

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KR20190024883A KR20190024883A (ko) 2019-03-08
KR102522263B1 true KR102522263B1 (ko) 2023-04-18

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US (3) US20200002690A1 (enExample)
EP (1) EP3469075A2 (enExample)
JP (2) JP7118510B2 (enExample)
KR (1) KR102522263B1 (enExample)
CN (1) CN109477080A (enExample)
AU (2) AU2017286477C1 (enExample)
CA (1) CA3024184A1 (enExample)
FR (2) FR3052462A1 (enExample)
IL (1) IL263503B2 (enExample)
SG (2) SG11201809961TA (enExample)
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Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10774316B2 (en) * 2014-10-20 2020-09-15 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
CA3064820A1 (en) * 2017-05-26 2018-11-29 Nuclera Nucleics Ltd Use of terminal transferase enzyme in nucleic acid synthesis
WO2019030149A1 (en) 2017-08-07 2019-02-14 Dna Script VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF
WO2019135007A1 (en) * 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
JP7430701B2 (ja) 2018-07-23 2024-02-13 ディーエヌエー スクリプト 核酸鎖の大規模並列酵素合成
EP3880808A1 (en) 2018-11-14 2021-09-22 DNA Script Terminal deoxynucleotidyl transferase variants and uses thereof
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
US12428668B2 (en) 2019-01-03 2025-09-30 Dna Script One pot synthesis of sets of oligonucleotides
WO2020146425A1 (en) 2019-01-07 2020-07-16 Elegen Corporation Methods of using microfluidic positional encoding devices
GB201901501D0 (en) * 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
WO2020165137A1 (en) 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CN114207140A (zh) * 2019-05-28 2022-03-18 Dna斯克瑞普特公司 末端脱氧核苷酸转移酶变体及其用途
EP3744854A1 (en) * 2019-05-28 2020-12-02 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2021018919A1 (en) 2019-07-30 2021-02-04 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
CN114430778B (zh) 2019-08-01 2024-07-05 Dna斯克瑞普特公司 增加多核苷酸的无模板酶促合成中长序列的产量
CN110331136B (zh) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
US20220315970A1 (en) 2019-09-09 2022-10-06 Dna Script Template-Free Enzymatic Polynucleotide Synthesis Using Photocleavable Linkages
JP2022549196A (ja) 2019-09-23 2022-11-24 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加
US20220411840A1 (en) 2019-11-13 2022-12-29 Dna Script High Efficiency Template-Free Enzymatic Synthesis of Polynucleotides
US20230062303A1 (en) 2019-12-12 2023-03-02 Dna Script Chimeric Terminal Deoxynucleotidyl Transferases For Template-Free Enzymatic Synthesis Of Polynucleotides
EP4077703A1 (en) 2019-12-16 2022-10-26 DNA Script Template-free enzymatic polynucleotide synthesis using dismutationless terminal deoxynucleotidyl transferase variants
EP4110940B1 (en) 2020-02-25 2024-03-27 DNA Script Method and apparatus for enzymatic synthesis of polynucleotides
KR20230002825A (ko) 2020-04-20 2023-01-05 디엔에이 스크립트 말단 데옥시뉴클레오티딜 트랜스퍼라제 변이체 및 그의 용도
US12209266B2 (en) 2020-05-12 2025-01-28 Illumina, Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
JP7828081B2 (ja) * 2020-05-12 2026-03-11 ナショナル ユニヴァーシティー オブ シンガポール 熱安定性末端デオキシヌクレオチジルトランスフェラーゼ
KR20240016237A (ko) * 2020-06-03 2024-02-06 몰레큘러 어셈블리즈, 인크. 폴리데옥시뉴클레오티드 합성을 위한 변형된 템플레이트-비의존성 효소
WO2021250265A2 (en) 2020-06-12 2021-12-16 Synhelix Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes
ES3070893T3 (en) 2020-06-16 2026-06-18 Dna Script Method for enzymatic polynucleotide synthesis
EP4182472A1 (en) 2020-07-15 2023-05-24 DNA Script Massively parallel enzymatic synthesis of polynucleotides
WO2022019836A1 (en) 2020-07-21 2022-01-27 Illumina Singapore Pte Ltd Base-modified nucleotides as substrates for tdt-based enzymatic nucleic acid
US20230357730A1 (en) * 2020-08-04 2023-11-09 Nuclera Ltd Modified Terminal Deoxynucleotidyl Transferase (TdT) Enzymes
GB2598152B (en) 2020-08-21 2025-04-16 Nuclera Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
AU2021347452A1 (en) 2020-09-22 2023-04-27 Dna Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
US20240052391A1 (en) 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
US12391970B2 (en) 2020-11-11 2025-08-19 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
CN112322715B (zh) * 2020-11-17 2022-11-25 清华大学 一种核酸测序方法
AU2022249826A1 (en) 2021-04-02 2023-09-21 Dna Script Methods and kits for enzymatic synthesis of g4-prone polynucleotides
DE102022114351A1 (de) 2021-06-10 2022-12-15 Dna Script Enzymatische synthese von polynukleotiden mittels 3'-o-amino-2'-deoxyribonukleosid triphosphat monomeren
US20240352443A1 (en) 2021-08-23 2024-10-24 Dna Script Method for generating double-stranded nucleic acid
EP4395933A1 (en) 2021-09-03 2024-07-10 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
WO2023069865A1 (en) 2021-10-15 2023-04-27 Codexis, Inc. Engineered terminal deoxynucleotidyl transferase variants
CA3236657A1 (en) 2021-11-10 2023-05-19 Daniel MCHALE Novel terminal deoxynucleotidyl transferase (tdt) variant and uses thereof
EP4430176A2 (en) 2021-11-10 2024-09-18 DNA Script Novel terminal deoxynucleotidyl transferase (tdt) variants
CN116555216A (zh) * 2022-01-28 2023-08-08 中国科学院天津工业生物技术研究所 可控合成单链dna的末端转移酶变体及应用
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170266A1 (en) 2022-03-11 2023-09-14 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023240202A2 (en) * 2022-06-08 2023-12-14 Ansa Biotechnologies, Inc. Modified terminal deoxynucleotidyl transferase polymerases
WO2024013278A1 (en) 2022-07-13 2024-01-18 Dna Script Method of purifying solution comprising polynucleotide
WO2024121022A1 (en) 2022-12-05 2024-06-13 Dna Script Variants of poly(a) polymerase and uses thereof
WO2024121360A1 (en) 2022-12-09 2024-06-13 Dna Script In vitro enzymatical rna synthesis
CN120603956A (zh) 2022-12-31 2025-09-05 Dna斯克瑞普特公司 用于酶试剂的喷墨递送的可变粘度油墨
WO2024153642A1 (en) 2023-01-16 2024-07-25 Dna Script Scarless template-free enzymatic synthesis of polynucleotides
CN121195075A (zh) 2023-01-16 2025-12-23 Dna斯克瑞普特公司 喷墨辅助的酶促核酸合成
EP4655411A2 (en) 2023-01-26 2025-12-03 DNA Script Enzymatic synthesis of polynucleotide probes
JP2026516019A (ja) 2023-05-03 2026-05-19 デエヌア・スクリプト ３’－ｏ－アミノポリヌクレオチドの脱保護に使用する試薬
WO2024241324A1 (en) * 2023-05-24 2024-11-28 Scintium Ltd Method for training an artificial intelligence (ai) model and generating a 3d computer-aided design (cad) and system therefor
WO2024256604A1 (en) 2023-06-15 2024-12-19 Dna Script Salt addition during enzymatic polynucleotide synthesis
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
EP4750906A1 (en) 2023-07-27 2026-06-03 DNA Script Method for generating a plurality of polynucleotides
WO2025088024A1 (en) 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
WO2025093659A1 (en) 2023-11-04 2025-05-08 Dna Script 3'-o-aminooxymethyl blocked nucleotides and use thereof in enzymatic synthesis of polynucleotides
CN122396768A (zh) * 2023-12-26 2026-07-14 华大工程生物学长荡湖研究所 一种末端脱氧核糖核苷转移酶突变体及其制备方法和应用
WO2025155239A1 (en) * 2024-01-17 2025-07-24 Agency For Science, Technology And Research X family dna polymerases and uses thereof
EP4653525A1 (en) * 2024-05-21 2025-11-26 Uab "Biomatter Designs" Engineered terminal deoxynucleotidyl transferase polymerases
WO2026008711A2 (en) 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis
WO2026008717A2 (en) 2024-07-02 2026-01-08 Dna Script Method for enzymatic synthesis of polynucleotides
WO2026017834A1 (en) 2024-07-19 2026-01-22 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis and cleavage

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160108382A1 (en) * 2014-10-20 2016-04-21 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004689A (en) 1982-02-22 1991-04-02 Biogen, Massachusetts DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields
IL71691A (en) 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
BG52073B2 (en) 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
WO2010062776A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
US20120244209A1 (en) * 2011-03-02 2012-09-27 Roth Jack A Tusc2 therapies
WO2015089080A2 (en) * 2013-12-09 2015-06-18 Adimab, Llc Polyclonal mixtures of antibodies, and methods of making and using them
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160108382A1 (en) * 2014-10-20 2016-04-21 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
WO2016064880A1 (en) 2014-10-20 2016-04-28 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
비특허문헌 1

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FR3053699A1 (fr) 2018-01-12
AU2017286477C1 (en) 2023-12-21
AU2023282219A1 (en) 2024-01-18
EP3469075A2 (fr) 2019-04-17
WO2017216472A3 (fr) 2018-03-15
US20230167421A1 (en) 2023-06-01
IL263503A (en) 2019-02-03
US20240124855A1 (en) 2024-04-18
WO2017216472A2 (fr) 2017-12-21
SG11201809961TA (en) 2018-12-28
IL263503B1 (en) 2024-12-01
CN109477080A (zh) 2019-03-15
JP7118510B2 (ja) 2022-08-16
AU2017286477B2 (en) 2023-09-14
IL263503B2 (en) 2025-04-01
SG10202009688QA (en) 2020-11-27
KR20190024883A (ko) 2019-03-08
FR3053699B1 (fr) 2021-04-02
JP2022137127A (ja) 2022-09-21
CA3024184A1 (en) 2017-12-21
JP2019517799A (ja) 2019-06-27
AU2017286477A1 (en) 2018-12-06
US20200002690A1 (en) 2020-01-02
FR3052462A1 (fr) 2017-12-15

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