JP2019510515A5 - - Google Patents
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- JP2019510515A5 JP2019510515A5 JP2019503197A JP2019503197A JP2019510515A5 JP 2019510515 A5 JP2019510515 A5 JP 2019510515A5 JP 2019503197 A JP2019503197 A JP 2019503197A JP 2019503197 A JP2019503197 A JP 2019503197A JP 2019510515 A5 JP2019510515 A5 JP 2019510515A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- oligonucleotide
- sequence
- item
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000039446 nucleic acids Human genes 0.000 claims description 142
- 108020004707 nucleic acids Proteins 0.000 claims description 142
- 150000007523 nucleic acids Chemical class 0.000 claims description 142
- 238000000034 method Methods 0.000 claims description 132
- 108091034117 Oligonucleotide Proteins 0.000 claims description 119
- 230000003321 amplification Effects 0.000 claims description 83
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 83
- 230000000295 complement effect Effects 0.000 claims description 72
- 108091033319 polynucleotide Proteins 0.000 claims description 70
- 102000040430 polynucleotide Human genes 0.000 claims description 70
- 239000002157 polynucleotide Substances 0.000 claims description 70
- 230000000694 effects Effects 0.000 claims description 54
- 239000002773 nucleotide Substances 0.000 claims description 27
- 125000003729 nucleotide group Chemical group 0.000 claims description 27
- 125000006850 spacer group Chemical group 0.000 claims description 21
- 108020004414 DNA Proteins 0.000 claims description 20
- 239000012634 fragment Substances 0.000 claims description 20
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 18
- 238000011901 isothermal amplification Methods 0.000 claims description 14
- 238000011897 real-time detection Methods 0.000 claims description 9
- 108060004795 Methyltransferase Proteins 0.000 claims description 8
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 108060002716 Exonuclease Proteins 0.000 claims description 6
- 102000018120 Recombinases Human genes 0.000 claims description 6
- 108010091086 Recombinases Proteins 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000003776 cleavage reaction Methods 0.000 claims description 6
- 102000013165 exonuclease Human genes 0.000 claims description 6
- 230000007017 scission Effects 0.000 claims description 6
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims description 3
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 101710162453 Replication factor A Proteins 0.000 claims description 3
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims description 3
- 101710176276 SSB protein Proteins 0.000 claims description 3
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims description 3
- 102100034343 Integrase Human genes 0.000 claims 2
- 239000000523 sample Substances 0.000 description 80
- 102100031780 Endonuclease Human genes 0.000 description 8
- 230000002255 enzymatic effect Effects 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 108020004513 Bacterial RNA Proteins 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108020000999 Viral RNA Proteins 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000012521 purified sample Substances 0.000 description 2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020092422A JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15/090,405 US9617587B1 (en) | 2016-04-04 | 2016-04-04 | Isothermal amplification components and processes |
| US15/090,405 | 2016-04-04 | ||
| PCT/US2017/020921 WO2017176404A1 (en) | 2016-04-04 | 2017-03-06 | Isothermal amplification components and processes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020092422A Division JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019510515A JP2019510515A (ja) | 2019-04-18 |
| JP2019510515A5 true JP2019510515A5 (https=) | 2020-03-19 |
| JP6758474B2 JP6758474B2 (ja) | 2020-09-23 |
Family
ID=58348016
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019503197A Active JP6758474B2 (ja) | 2016-04-04 | 2017-03-06 | 等温増幅の成分およびプロセス |
| JP2020092422A Active JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
| JP2022077421A Active JP7571080B2 (ja) | 2016-04-04 | 2022-05-10 | 等温増幅の成分およびプロセス |
| JP2024087154A Pending JP2024114703A (ja) | 2016-04-04 | 2024-05-29 | 等温増幅の成分およびプロセス |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020092422A Active JP7426901B2 (ja) | 2016-04-04 | 2020-05-27 | 等温増幅の成分およびプロセス |
| JP2022077421A Active JP7571080B2 (ja) | 2016-04-04 | 2022-05-10 | 等温増幅の成分およびプロセス |
| JP2024087154A Pending JP2024114703A (ja) | 2016-04-04 | 2024-05-29 | 等温増幅の成分およびプロセス |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US9617587B1 (https=) |
| EP (4) | EP3760735B1 (https=) |
| JP (4) | JP6758474B2 (https=) |
| CN (1) | CN109196114A (https=) |
| AU (2) | AU2017247133B2 (https=) |
| CA (1) | CA3018202A1 (https=) |
| ES (3) | ES2940071T3 (https=) |
| WO (1) | WO2017176404A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| US9617587B1 (en) * | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| KR101884793B1 (ko) * | 2017-11-23 | 2018-08-02 | 강원대학교산학협력단 | Cage 단백질의 생산 및 정제 방법 |
| CN110558279B (zh) * | 2019-09-16 | 2021-11-16 | 南方科技大学 | 一种药物筛选模型、药物筛选方法及其应用 |
| EP4308724A4 (en) * | 2021-03-19 | 2025-05-14 | Becton, Dickinson and Company | ISOTHERMAL AMPLIFICATION OF PATHOGENS |
| WO2023150710A1 (en) | 2022-02-05 | 2023-08-10 | Becton, Dickinson And Company | Non-opaque lytic buffer composition formulations |
| US20250059612A1 (en) | 2022-02-05 | 2025-02-20 | Becton, Dickinson And Company | Method for separating genomic dna for amplification of short nucleic acid targets |
| JP2025507309A (ja) | 2022-02-14 | 2025-03-18 | ベクトン・ディキンソン・アンド・カンパニー | 核酸増幅のための内部対照 |
| CN120019163A (zh) * | 2022-09-07 | 2025-05-16 | 贝克顿迪金森公司 | 古细菌聚合酶扩增 |
| EP4584392A1 (en) | 2022-09-07 | 2025-07-16 | Becton, Dickinson and Company | Hairpin internal control for isothermal nucleic acid amplification |
| JP2025531820A (ja) | 2022-09-07 | 2025-09-25 | ベクトン・ディキンソン・アンド・カンパニー | 検出特異性改善のための修飾分子ビーコン |
| EP4627112A1 (en) | 2022-12-01 | 2025-10-08 | Becton, Dickinson and Company | Asymmetric hairpin probes for nucleic acid detection |
| CN116286720A (zh) * | 2023-03-29 | 2023-06-23 | 赛纳生物科技(北京)有限公司 | 一种dna聚合酶 |
| WO2024216077A1 (en) | 2023-04-13 | 2024-10-17 | Becton, Dickinson And Company | Method for categorizing molecular diagnostic signals in real time |
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-
2016
- 2016-04-04 US US15/090,405 patent/US9617587B1/en active Active
-
2017
- 2017-03-06 AU AU2017247133A patent/AU2017247133B2/en active Active
- 2017-03-06 ES ES20182776T patent/ES2940071T3/es active Active
- 2017-03-06 WO PCT/US2017/020921 patent/WO2017176404A1/en not_active Ceased
- 2017-03-06 JP JP2019503197A patent/JP6758474B2/ja active Active
- 2017-03-06 EP EP20182776.3A patent/EP3760735B1/en active Active
- 2017-03-06 CA CA3018202A patent/CA3018202A1/en active Pending
- 2017-03-06 EP EP17711527.6A patent/EP3440217B1/en active Active
- 2017-03-06 ES ES22165594T patent/ES2988526T3/es active Active
- 2017-03-06 EP EP24156601.7A patent/EP4389910B1/en active Active
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