JP2019213545A5 - - Google Patents
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- Publication number
- JP2019213545A5 JP2019213545A5 JP2019143116A JP2019143116A JP2019213545A5 JP 2019213545 A5 JP2019213545 A5 JP 2019213545A5 JP 2019143116 A JP2019143116 A JP 2019143116A JP 2019143116 A JP2019143116 A JP 2019143116A JP 2019213545 A5 JP2019213545 A5 JP 2019213545A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinase
- amino acid
- seq
- uvsx
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000018120 Recombinases Human genes 0.000 claims 47
- 108010091086 Recombinases Proteins 0.000 claims 47
- 125000003275 alpha amino acid group Chemical group 0.000 claims 23
- 230000003321 amplification Effects 0.000 claims 19
- 238000003199 nucleic acid amplification method Methods 0.000 claims 19
- 108020004707 nucleic acids Proteins 0.000 claims 16
- 102000039446 nucleic acids Human genes 0.000 claims 16
- 150000007523 nucleic acids Chemical class 0.000 claims 16
- 238000000034 method Methods 0.000 claims 13
- 238000006467 substitution reaction Methods 0.000 claims 12
- 241000305071 Enterobacterales Species 0.000 claims 9
- 230000035772 mutation Effects 0.000 claims 5
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 4
- 239000004472 Lysine Substances 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 235000018102 proteins Nutrition 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 101800001466 Envelope glycoprotein E1 Proteins 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 2
- 241000701533 Escherichia virus T4 Species 0.000 claims 2
- 102000011931 Nucleoproteins Human genes 0.000 claims 2
- 108010061100 Nucleoproteins Proteins 0.000 claims 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 2
- 101800001690 Transmembrane protein gp41 Proteins 0.000 claims 2
- 235000001014 amino acid Nutrition 0.000 claims 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 102100027211 Albumin Human genes 0.000 claims 1
- 108010088751 Albumins Proteins 0.000 claims 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 1
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 229920002307 Dextran Polymers 0.000 claims 1
- 101000587453 Escherichia coli Plasmid-derived single-stranded DNA-binding protein Proteins 0.000 claims 1
- 101000587458 Escherichia coli Plasmid-derived single-stranded DNA-binding protein Proteins 0.000 claims 1
- 101000700922 Escherichia coli Plasmid-derived single-stranded DNA-binding protein Proteins 0.000 claims 1
- 101000826084 Escherichia coli Plasmid-derived single-stranded DNA-binding protein Proteins 0.000 claims 1
- 229920001917 Ficoll Polymers 0.000 claims 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- 101710162453 Replication factor A Proteins 0.000 claims 1
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims 1
- 101710176276 SSB protein Proteins 0.000 claims 1
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical group 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000003638 chemical reducing agent Substances 0.000 claims 1
- 239000000839 emulsion Substances 0.000 claims 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims 1
- 101150103919 recO gene Proteins 0.000 claims 1
- 101150033993 recR gene Proteins 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462057056P | 2014-09-29 | 2014-09-29 | |
| US62/057,056 | 2014-09-29 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017516936A Division JP2017529093A (ja) | 2014-09-29 | 2015-09-29 | リコンビナーゼ変異体 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019213545A JP2019213545A (ja) | 2019-12-19 |
| JP2019213545A5 true JP2019213545A5 (https=) | 2020-07-16 |
| JP6879519B2 JP6879519B2 (ja) | 2021-06-02 |
Family
ID=54293382
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017516936A Pending JP2017529093A (ja) | 2014-09-29 | 2015-09-29 | リコンビナーゼ変異体 |
| JP2019143116A Active JP6879519B2 (ja) | 2014-09-29 | 2019-08-02 | リコンビナーゼ変異体 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017516936A Pending JP2017529093A (ja) | 2014-09-29 | 2015-09-29 | リコンビナーゼ変異体 |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US9982244B2 (https=) |
| EP (1) | EP3201222B1 (https=) |
| JP (2) | JP2017529093A (https=) |
| KR (3) | KR102126641B1 (https=) |
| CN (1) | CN107208075B (https=) |
| AU (1) | AU2015323936B2 (https=) |
| BR (1) | BR112017006542B1 (https=) |
| CA (1) | CA2962941C (https=) |
| DK (1) | DK3201222T3 (https=) |
| ES (1) | ES2729302T3 (https=) |
| IL (1) | IL251422B (https=) |
| MX (1) | MX385063B (https=) |
| MY (1) | MY182395A (https=) |
| NZ (1) | NZ730593A (https=) |
| RU (1) | RU2721920C2 (https=) |
| SG (1) | SG11201702493WA (https=) |
| WO (1) | WO2016054088A1 (https=) |
| ZA (1) | ZA201702248B (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102126641B1 (ko) * | 2014-09-29 | 2020-06-24 | 일루미나 케임브리지 리미티드 | 재조합효소 돌연변이체 |
| AU2017241670B2 (en) | 2016-03-28 | 2020-10-08 | Illumina, Inc. | Recombinase mutants |
| US11268137B2 (en) | 2016-12-09 | 2022-03-08 | Boreal Genomics, Inc. | Linked ligation |
| GB201704754D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
| CN108913700B (zh) * | 2018-08-16 | 2021-04-09 | 深圳市艾伟迪生物科技有限公司 | gp32单链结合蛋白的制备方法、表达基因、重组表达载体及应用 |
| CN115975956A (zh) * | 2022-02-23 | 2023-04-18 | 杨挥 | 一种重组酶突变体及包含该突变体的核酸等温扩增反应体系 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US7270981B2 (en) | 2002-02-21 | 2007-09-18 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US6897069B1 (en) | 2004-06-08 | 2005-05-24 | Ambion, Inc. | System and method for electroporating a sample |
| WO2007096702A2 (en) | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| US7742463B2 (en) | 2005-08-18 | 2010-06-22 | Hong Kong Applied Science And Technology Research Institute Co., Ltd. | Security gatekeeper for a packetized voice communication network |
| US8071308B2 (en) * | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| EP2438196B1 (en) * | 2009-06-05 | 2016-12-21 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| US8563748B2 (en) | 2010-09-21 | 2013-10-22 | Basf Se | Process for preparing substituted N-phenylhydroxylamines |
| EP2694666B1 (en) * | 2011-04-07 | 2018-05-23 | Alere San Diego, Inc. | Monitoring recombinase polymerase amplification mixtures |
| US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| KR102126641B1 (ko) * | 2014-09-29 | 2020-06-24 | 일루미나 케임브리지 리미티드 | 재조합효소 돌연변이체 |
-
2015
- 2015-09-29 KR KR1020197008943A patent/KR102126641B1/ko active Active
- 2015-09-29 DK DK15779119.5T patent/DK3201222T3/da active
- 2015-09-29 EP EP15779119.5A patent/EP3201222B1/en active Active
- 2015-09-29 WO PCT/US2015/053012 patent/WO2016054088A1/en not_active Ceased
- 2015-09-29 US US14/869,744 patent/US9982244B2/en active Active
- 2015-09-29 CN CN201580059870.9A patent/CN107208075B/zh active Active
- 2015-09-29 SG SG11201702493WA patent/SG11201702493WA/en unknown
- 2015-09-29 KR KR1020207006203A patent/KR20200026320A/ko not_active Ceased
- 2015-09-29 MY MYPI2017701081A patent/MY182395A/en unknown
- 2015-09-29 AU AU2015323936A patent/AU2015323936B2/en active Active
- 2015-09-29 MX MX2017004034A patent/MX385063B/es unknown
- 2015-09-29 KR KR1020177011954A patent/KR20170065028A/ko not_active Ceased
- 2015-09-29 JP JP2017516936A patent/JP2017529093A/ja active Pending
- 2015-09-29 RU RU2017110582A patent/RU2721920C2/ru active
- 2015-09-29 CA CA2962941A patent/CA2962941C/en active Active
- 2015-09-29 ES ES15779119T patent/ES2729302T3/es active Active
- 2015-09-29 NZ NZ730593A patent/NZ730593A/en unknown
- 2015-09-29 BR BR112017006542-8A patent/BR112017006542B1/pt active IP Right Grant
-
2017
- 2017-03-27 IL IL251422A patent/IL251422B/en active IP Right Grant
- 2017-03-30 ZA ZA2017/02248A patent/ZA201702248B/en unknown
-
2018
- 2018-05-11 US US15/977,389 patent/US10344269B2/en active Active
-
2019
- 2019-08-02 JP JP2019143116A patent/JP6879519B2/ja active Active
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