ES2729302T3 - Mutantes de recombinasas - Google Patents

Mutantes de recombinasas Download PDF

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Publication number
ES2729302T3
ES2729302T3 ES15779119T ES15779119T ES2729302T3 ES 2729302 T3 ES2729302 T3 ES 2729302T3 ES 15779119 T ES15779119 T ES 15779119T ES 15779119 T ES15779119 T ES 15779119T ES 2729302 T3 ES2729302 T3 ES 2729302T3
Authority
ES
Spain
Prior art keywords
uvsx
nucleic acid
seq
recombinase
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES15779119T
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English (en)
Spanish (es)
Inventor
Erin Bomati
Matthew William Kellinger
Jonathan Mark Boutell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Cambridge Ltd
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of ES2729302T3 publication Critical patent/ES2729302T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01DNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y306/00Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01008ATP diphosphatase (3.6.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/501Ligase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES15779119T 2014-09-29 2015-09-29 Mutantes de recombinasas Active ES2729302T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462057056P 2014-09-29 2014-09-29
PCT/US2015/053012 WO2016054088A1 (en) 2014-09-29 2015-09-29 Recombinase mutants

Publications (1)

Publication Number Publication Date
ES2729302T3 true ES2729302T3 (es) 2019-10-31

Family

ID=54293382

Family Applications (1)

Application Number Title Priority Date Filing Date
ES15779119T Active ES2729302T3 (es) 2014-09-29 2015-09-29 Mutantes de recombinasas

Country Status (18)

Country Link
US (2) US9982244B2 (https=)
EP (1) EP3201222B1 (https=)
JP (2) JP2017529093A (https=)
KR (3) KR102126641B1 (https=)
CN (1) CN107208075B (https=)
AU (1) AU2015323936B2 (https=)
BR (1) BR112017006542B1 (https=)
CA (1) CA2962941C (https=)
DK (1) DK3201222T3 (https=)
ES (1) ES2729302T3 (https=)
IL (1) IL251422B (https=)
MX (1) MX385063B (https=)
MY (1) MY182395A (https=)
NZ (1) NZ730593A (https=)
RU (1) RU2721920C2 (https=)
SG (1) SG11201702493WA (https=)
WO (1) WO2016054088A1 (https=)
ZA (1) ZA201702248B (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102126641B1 (ko) * 2014-09-29 2020-06-24 일루미나 케임브리지 리미티드 재조합효소 돌연변이체
AU2017241670B2 (en) * 2016-03-28 2020-10-08 Illumina, Inc. Recombinase mutants
JP7048609B2 (ja) * 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN108913700B (zh) * 2018-08-16 2021-04-09 深圳市艾伟迪生物科技有限公司 gp32单链结合蛋白的制备方法、表达基因、重组表达载体及应用
CN115975956A (zh) * 2022-02-23 2023-04-18 杨挥 一种重组酶突变体及包含该突变体的核酸等温扩增反应体系

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
US7742463B2 (en) 2005-08-18 2010-06-22 Hong Kong Applied Science And Technology Research Institute Co., Ltd. Security gatekeeper for a packetized voice communication network
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
ES3022638T3 (en) * 2009-06-05 2025-05-28 Abbott Diagnostics Scarborough Inc Recombinase polymerase amplification reagents
EP2619183B1 (en) 2010-09-21 2016-12-07 Basf Se Process for preparing substituted n-phenylhydroxylamines
BR112013025758B1 (pt) * 2011-04-07 2021-04-06 Abbott Diagnostics Scarborough, Inc. Monitoramento de misturas de amplificação de recombinase polimerase
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
KR102126641B1 (ko) * 2014-09-29 2020-06-24 일루미나 케임브리지 리미티드 재조합효소 돌연변이체

Also Published As

Publication number Publication date
MX385063B (es) 2025-03-14
CA2962941C (en) 2022-06-14
CN107208075B (zh) 2021-08-24
JP2017529093A (ja) 2017-10-05
DK3201222T3 (da) 2019-06-24
AU2015323936B2 (en) 2021-03-11
EP3201222A1 (en) 2017-08-09
RU2017110582A (ru) 2018-11-07
RU2721920C2 (ru) 2020-05-25
KR20170065028A (ko) 2017-06-12
BR112017006542A2 (pt) 2018-01-23
KR20200026320A (ko) 2020-03-10
JP6879519B2 (ja) 2021-06-02
BR112017006542B1 (pt) 2023-11-14
US9982244B2 (en) 2018-05-29
US10344269B2 (en) 2019-07-09
EP3201222B1 (en) 2019-04-10
KR20190037364A (ko) 2019-04-05
IL251422B (en) 2020-10-29
RU2017110582A3 (https=) 2019-04-15
US20180258409A1 (en) 2018-09-13
MX2017004034A (es) 2017-11-17
CA2962941A1 (en) 2016-04-07
KR102126641B1 (ko) 2020-06-24
NZ730593A (en) 2023-06-30
CN107208075A (zh) 2017-09-26
IL251422A0 (en) 2017-05-29
WO2016054088A1 (en) 2016-04-07
JP2019213545A (ja) 2019-12-19
SG11201702493WA (en) 2017-04-27
AU2015323936A1 (en) 2017-04-20
ZA201702248B (en) 2019-06-26
US20160090581A1 (en) 2016-03-31
MY182395A (en) 2021-01-23

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