BR112017006542A2 - mutantes de recombinase - Google Patents

mutantes de recombinase

Info

Publication number
BR112017006542A2
BR112017006542A2 BR112017006542-8A BR112017006542A BR112017006542A2 BR 112017006542 A2 BR112017006542 A2 BR 112017006542A2 BR 112017006542 A BR112017006542 A BR 112017006542A BR 112017006542 A2 BR112017006542 A2 BR 112017006542A2
Authority
BR
Brazil
Prior art keywords
methods
enhanced
recombinase mutants
recombinase
amplification
Prior art date
Application number
BR112017006542-8A
Other languages
English (en)
Other versions
BR112017006542B1 (pt
Inventor
BOMATI Erin
William KELLINGER Matthew
Mark Boutell Jonathan
Original Assignee
Illumina Cambridge Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Limited filed Critical Illumina Cambridge Limited
Publication of BR112017006542A2 publication Critical patent/BR112017006542A2/pt
Publication of BR112017006542B1 publication Critical patent/BR112017006542B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01008—ATP diphosphatase (3.6.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

Abstract

a presente invenção se refere a recombinases para amplificação de ácidos nucleicos mediada por recombinase aprimoradas, tal como uma biblioteca de pcr que possui regiões de adaptadores de cadeias únicas, em uma superfície de células de fluxo padronizado para amplificação de agrupamento aprimorado, assim como métodos e kits utilizado as mesmas.
BR112017006542-8A 2014-09-29 2015-09-29 Mutantes de recombinase BR112017006542B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462057056P 2014-09-29 2014-09-29
US62/057,056 2014-09-29
PCT/US2015/053012 WO2016054088A1 (en) 2014-09-29 2015-09-29 Recombinase mutants

Publications (2)

Publication Number Publication Date
BR112017006542A2 true BR112017006542A2 (pt) 2018-01-23
BR112017006542B1 BR112017006542B1 (pt) 2023-11-14

Family

ID=54293382

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017006542-8A BR112017006542B1 (pt) 2014-09-29 2015-09-29 Mutantes de recombinase

Country Status (18)

Country Link
US (2) US9982244B2 (pt)
EP (1) EP3201222B1 (pt)
JP (2) JP2017529093A (pt)
KR (3) KR20170065028A (pt)
CN (1) CN107208075B (pt)
AU (1) AU2015323936B2 (pt)
BR (1) BR112017006542B1 (pt)
CA (1) CA2962941C (pt)
DK (1) DK3201222T3 (pt)
ES (1) ES2729302T3 (pt)
IL (1) IL251422B (pt)
MX (1) MX2017004034A (pt)
MY (1) MY182395A (pt)
NZ (1) NZ730593A (pt)
RU (1) RU2721920C2 (pt)
SG (1) SG11201702493WA (pt)
WO (1) WO2016054088A1 (pt)
ZA (1) ZA201702248B (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170065028A (ko) * 2014-09-29 2017-06-12 일루미나 케임브리지 리미티드 재조합효소 돌연변이체
CN108779445A (zh) * 2016-03-28 2018-11-09 亿明达股份有限公司 重组酶突变
US11268137B2 (en) * 2016-12-09 2022-03-08 Boreal Genomics, Inc. Linked ligation
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN108913700B (zh) * 2018-08-16 2021-04-09 深圳市艾伟迪生物科技有限公司 gp32单链结合蛋白的制备方法、表达基因、重组表达载体及应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
ATE400663T1 (de) 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
EP1929049B1 (en) 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US7742463B2 (en) 2005-08-18 2010-06-22 Hong Kong Applied Science And Technology Research Institute Co., Ltd. Security gatekeeper for a packetized voice communication network
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
BR112013006230C8 (pt) 2010-09-21 2017-09-19 Basf Se processo para a preparação de uma n-fenilhidroxilamina substituída
JP5961247B2 (ja) * 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
KR20170065028A (ko) * 2014-09-29 2017-06-12 일루미나 케임브리지 리미티드 재조합효소 돌연변이체

Also Published As

Publication number Publication date
RU2721920C2 (ru) 2020-05-25
JP2019213545A (ja) 2019-12-19
JP6879519B2 (ja) 2021-06-02
KR20200026320A (ko) 2020-03-10
BR112017006542B1 (pt) 2023-11-14
US9982244B2 (en) 2018-05-29
AU2015323936A1 (en) 2017-04-20
CN107208075A (zh) 2017-09-26
WO2016054088A1 (en) 2016-04-07
MY182395A (en) 2021-01-23
US20160090581A1 (en) 2016-03-31
AU2015323936B2 (en) 2021-03-11
US10344269B2 (en) 2019-07-09
IL251422B (en) 2020-10-29
CN107208075B (zh) 2021-08-24
CA2962941C (en) 2022-06-14
RU2017110582A (ru) 2018-11-07
ZA201702248B (en) 2019-06-26
US20180258409A1 (en) 2018-09-13
MX2017004034A (es) 2017-11-17
KR20170065028A (ko) 2017-06-12
NZ730593A (en) 2023-06-30
EP3201222B1 (en) 2019-04-10
CA2962941A1 (en) 2016-04-07
EP3201222A1 (en) 2017-08-09
SG11201702493WA (en) 2017-04-27
KR20190037364A (ko) 2019-04-05
KR102126641B1 (ko) 2020-06-24
ES2729302T3 (es) 2019-10-31
IL251422A0 (en) 2017-05-29
DK3201222T3 (da) 2019-06-24
JP2017529093A (ja) 2017-10-05
RU2017110582A3 (pt) 2019-04-15

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Legal Events

Date Code Title Description
B25G Requested change of headquarter approved

Owner name: ILLUMINA CAMBRIDGE LIMITED (GB)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 29/09/2015, OBSERVADAS AS CONDICOES LEGAIS