CN107208075B - 重组酶突变体 - Google Patents

重组酶突变体 Download PDF

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Publication number
CN107208075B
CN107208075B CN201580059870.9A CN201580059870A CN107208075B CN 107208075 B CN107208075 B CN 107208075B CN 201580059870 A CN201580059870 A CN 201580059870A CN 107208075 B CN107208075 B CN 107208075B
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Chinese (zh)
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CN107208075A (zh
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E.博马蒂
M.W.凯林格
J.M.鲍特尔
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C12Y306/01008—ATP diphosphatase (3.6.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580059870.9A 2014-09-29 2015-09-29 重组酶突变体 Active CN107208075B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462057056P 2014-09-29 2014-09-29
US62/057,056 2014-09-29
PCT/US2015/053012 WO2016054088A1 (en) 2014-09-29 2015-09-29 Recombinase mutants

Publications (2)

Publication Number Publication Date
CN107208075A CN107208075A (zh) 2017-09-26
CN107208075B true CN107208075B (zh) 2021-08-24

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CN201580059870.9A Active CN107208075B (zh) 2014-09-29 2015-09-29 重组酶突变体

Country Status (18)

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US (2) US9982244B2 (https=)
EP (1) EP3201222B1 (https=)
JP (2) JP2017529093A (https=)
KR (3) KR102126641B1 (https=)
CN (1) CN107208075B (https=)
AU (1) AU2015323936B2 (https=)
BR (1) BR112017006542B1 (https=)
CA (1) CA2962941C (https=)
DK (1) DK3201222T3 (https=)
ES (1) ES2729302T3 (https=)
IL (1) IL251422B (https=)
MX (1) MX385063B (https=)
MY (1) MY182395A (https=)
NZ (1) NZ730593A (https=)
RU (1) RU2721920C2 (https=)
SG (1) SG11201702493WA (https=)
WO (1) WO2016054088A1 (https=)
ZA (1) ZA201702248B (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102126641B1 (ko) * 2014-09-29 2020-06-24 일루미나 케임브리지 리미티드 재조합효소 돌연변이체
AU2017241670B2 (en) * 2016-03-28 2020-10-08 Illumina, Inc. Recombinase mutants
JP7048609B2 (ja) * 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN108913700B (zh) * 2018-08-16 2021-04-09 深圳市艾伟迪生物科技有限公司 gp32单链结合蛋白的制备方法、表达基因、重组表达载体及应用
CN115975956A (zh) * 2022-02-23 2023-04-18 杨挥 一种重组酶突变体及包含该突变体的核酸等温扩增反应体系

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008035205A2 (en) * 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2013188582A1 (en) * 2012-06-15 2013-12-19 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
US7742463B2 (en) 2005-08-18 2010-06-22 Hong Kong Applied Science And Technology Research Institute Co., Ltd. Security gatekeeper for a packetized voice communication network
EP2619183B1 (en) 2010-09-21 2016-12-07 Basf Se Process for preparing substituted n-phenylhydroxylamines
BR112013025758B1 (pt) * 2011-04-07 2021-04-06 Abbott Diagnostics Scarborough, Inc. Monitoramento de misturas de amplificação de recombinase polimerase
KR102126641B1 (ko) * 2014-09-29 2020-06-24 일루미나 케임브리지 리미티드 재조합효소 돌연변이체

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008035205A2 (en) * 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
WO2010141940A1 (en) * 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
WO2013188582A1 (en) * 2012-06-15 2013-12-19 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries

Also Published As

Publication number Publication date
MX385063B (es) 2025-03-14
CA2962941C (en) 2022-06-14
JP2017529093A (ja) 2017-10-05
DK3201222T3 (da) 2019-06-24
ES2729302T3 (es) 2019-10-31
AU2015323936B2 (en) 2021-03-11
EP3201222A1 (en) 2017-08-09
RU2017110582A (ru) 2018-11-07
RU2721920C2 (ru) 2020-05-25
KR20170065028A (ko) 2017-06-12
BR112017006542A2 (pt) 2018-01-23
KR20200026320A (ko) 2020-03-10
JP6879519B2 (ja) 2021-06-02
BR112017006542B1 (pt) 2023-11-14
US9982244B2 (en) 2018-05-29
US10344269B2 (en) 2019-07-09
EP3201222B1 (en) 2019-04-10
KR20190037364A (ko) 2019-04-05
IL251422B (en) 2020-10-29
RU2017110582A3 (https=) 2019-04-15
US20180258409A1 (en) 2018-09-13
MX2017004034A (es) 2017-11-17
CA2962941A1 (en) 2016-04-07
KR102126641B1 (ko) 2020-06-24
NZ730593A (en) 2023-06-30
CN107208075A (zh) 2017-09-26
IL251422A0 (en) 2017-05-29
WO2016054088A1 (en) 2016-04-07
JP2019213545A (ja) 2019-12-19
SG11201702493WA (en) 2017-04-27
AU2015323936A1 (en) 2017-04-20
ZA201702248B (en) 2019-06-26
US20160090581A1 (en) 2016-03-31
MY182395A (en) 2021-01-23

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