JP2019022496A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2019022496A5 JP2019022496A5 JP2018169154A JP2018169154A JP2019022496A5 JP 2019022496 A5 JP2019022496 A5 JP 2019022496A5 JP 2018169154 A JP2018169154 A JP 2018169154A JP 2018169154 A JP2018169154 A JP 2018169154A JP 2019022496 A5 JP2019022496 A5 JP 2019022496A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- cells
- endoderm
- derived
- primate pluripotent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims description 216
- 210000001900 endoderm Anatomy 0.000 claims description 58
- 210000001161 mammalian embryo Anatomy 0.000 claims description 46
- 230000004069 differentiation Effects 0.000 claims description 43
- 210000004039 endoderm cell Anatomy 0.000 claims description 41
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims description 30
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 20
- 239000000725 suspension Substances 0.000 claims description 16
- 101000652324 Homo sapiens Transcription factor SOX-17 Proteins 0.000 claims description 14
- 102100030243 Transcription factor SOX-17 Human genes 0.000 claims description 14
- 239000003550 marker Substances 0.000 claims description 12
- -1 HNF3β Proteins 0.000 claims description 8
- 101000642523 Homo sapiens Transcription factor SOX-7 Proteins 0.000 claims description 7
- 102100036730 Transcription factor SOX-7 Human genes 0.000 claims description 7
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 7
- 102000045246 noggin Human genes 0.000 claims description 6
- 108700007229 noggin Proteins 0.000 claims description 6
- 102100022123 Hepatocyte nuclear factor 1-beta Human genes 0.000 claims description 5
- 101001045758 Homo sapiens Hepatocyte nuclear factor 1-beta Proteins 0.000 claims description 5
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 claims description 5
- 102100038553 Neurogenin-3 Human genes 0.000 claims description 5
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims description 4
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims description 4
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims description 4
- 101000976653 Homo sapiens Zinc finger protein ZIC 1 Proteins 0.000 claims description 4
- 102100023497 Zinc finger protein ZIC 1 Human genes 0.000 claims description 4
- 101100518002 Danio rerio nkx2.2a gene Proteins 0.000 claims description 3
- 108700014808 Homeobox Protein Nkx-2.2 Proteins 0.000 claims description 3
- 102100027886 Homeobox protein Nkx-2.2 Human genes 0.000 claims description 3
- 101100460496 Homo sapiens NKX2-2 gene Proteins 0.000 claims description 3
- 101000738523 Homo sapiens Pancreas transcription factor 1 subunit alpha Proteins 0.000 claims description 3
- 108010032788 PAX6 Transcription Factor Proteins 0.000 claims description 3
- 102100037506 Paired box protein Pax-6 Human genes 0.000 claims description 3
- 102100037878 Pancreas transcription factor 1 subunit alpha Human genes 0.000 claims description 3
- 239000006285 cell suspension Substances 0.000 claims description 3
- 210000002894 multi-fate stem cell Anatomy 0.000 claims description 3
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 2
- 229930002330 retinoic acid Natural products 0.000 claims description 2
- 229960001727 tretinoin Drugs 0.000 claims description 2
- 241000288906 Primates Species 0.000 claims 15
- 239000000411 inducer Substances 0.000 claims 1
- 230000014509 gene expression Effects 0.000 description 47
- 210000002438 upper gastrointestinal tract Anatomy 0.000 description 21
- 210000000130 stem cell Anatomy 0.000 description 17
- 238000004519 manufacturing process Methods 0.000 description 16
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 108010007718 Chromogranins Proteins 0.000 description 13
- 102000007345 Chromogranins Human genes 0.000 description 13
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 13
- 210000001671 embryonic stem cell Anatomy 0.000 description 13
- 238000004114 suspension culture Methods 0.000 description 12
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 11
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 10
- 239000012091 fetal bovine serum Substances 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 9
- 230000001464 adherent effect Effects 0.000 description 9
- 239000003636 conditioned culture medium Substances 0.000 description 9
- 210000003890 endocrine cell Anatomy 0.000 description 9
- 239000003102 growth factor Substances 0.000 description 9
- 102100035290 Fibroblast growth factor 13 Human genes 0.000 description 8
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 8
- 101150068639 Hnf4a gene Proteins 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 108010023082 activin A Proteins 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 210000002242 embryoid body Anatomy 0.000 description 7
- 210000001654 germ layer Anatomy 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000004113 cell culture Methods 0.000 description 6
- 230000024245 cell differentiation Effects 0.000 description 6
- 238000000684 flow cytometry Methods 0.000 description 6
- 230000009996 pancreatic endocrine effect Effects 0.000 description 6
- 239000002243 precursor Substances 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 5
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 5
- 108010076089 accutase Proteins 0.000 description 5
- 230000002124 endocrine Effects 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- 102100029284 Hepatocyte nuclear factor 3-beta Human genes 0.000 description 4
- 101001062347 Homo sapiens Hepatocyte nuclear factor 3-beta Proteins 0.000 description 4
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 102400000058 Neuregulin-1 Human genes 0.000 description 4
- 102000052651 Pancreatic hormone Human genes 0.000 description 4
- 108010009583 Transforming Growth Factors Proteins 0.000 description 4
- 102000009618 Transforming Growth Factors Human genes 0.000 description 4
- 238000004115 adherent culture Methods 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 238000013401 experimental design Methods 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 210000003716 mesoderm Anatomy 0.000 description 4
- 239000004025 pancreas hormone Substances 0.000 description 4
- 229940032957 pancreatic hormone Drugs 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 3
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 3
- 101800001268 Pancreatic hormone Proteins 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 210000003494 hepatocyte Anatomy 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 108010083123 CDX2 Transcription Factor Proteins 0.000 description 2
- 102100037124 Developmental pluripotency-associated 5 protein Human genes 0.000 description 2
- 102100031116 Disintegrin and metalloproteinase domain-containing protein 19 Human genes 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102100037060 Forkhead box protein D3 Human genes 0.000 description 2
- 239000012571 GlutaMAX medium Substances 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 102100031671 Homeobox protein CDX-2 Human genes 0.000 description 2
- 101000881848 Homo sapiens Developmental pluripotency-associated 5 protein Proteins 0.000 description 2
- 101000777464 Homo sapiens Disintegrin and metalloproteinase domain-containing protein 19 Proteins 0.000 description 2
- 101001029308 Homo sapiens Forkhead box protein D3 Proteins 0.000 description 2
- 101000942967 Homo sapiens Leukemia inhibitory factor Proteins 0.000 description 2
- 101000835745 Homo sapiens Teratocarcinoma-derived growth factor 1 Proteins 0.000 description 2
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 101100445364 Mus musculus Eomes gene Proteins 0.000 description 2
- 108090000556 Neuregulin-1 Proteins 0.000 description 2
- 108010070047 Notch Receptors Proteins 0.000 description 2
- 101710150336 Protein Rex Proteins 0.000 description 2
- 238000001190 Q-PCR Methods 0.000 description 2
- 238000010240 RT-PCR analysis Methods 0.000 description 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 2
- 102100026404 Teratocarcinoma-derived growth factor 1 Human genes 0.000 description 2
- 102100024270 Transcription factor SOX-2 Human genes 0.000 description 2
- 102000044880 Wnt3A Human genes 0.000 description 2
- 108700013515 Wnt3A Proteins 0.000 description 2
- 101100445365 Xenopus laevis eomes gene Proteins 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000003981 ectoderm Anatomy 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004578 fetal growth Effects 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 102000046645 human LIF Human genes 0.000 description 2
- 210000003917 human chromosome Anatomy 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 108010082117 matrigel Proteins 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 210000002262 tip cell Anatomy 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- UBWXUGDQUBIEIZ-UHFFFAOYSA-N (13-methyl-3-oxo-2,6,7,8,9,10,11,12,14,15,16,17-dodecahydro-1h-cyclopenta[a]phenanthren-17-yl) 3-phenylpropanoate Chemical compound CC12CCC(C3CCC(=O)C=C3CC3)C3C1CCC2OC(=O)CCC1=CC=CC=C1 UBWXUGDQUBIEIZ-UHFFFAOYSA-N 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 108010059616 Activins Proteins 0.000 description 1
- 102000005606 Activins Human genes 0.000 description 1
- 101100454433 Biomphalaria glabrata BG01 gene Proteins 0.000 description 1
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 description 1
- 101100459234 Caenorhabditis elegans mxl-1 gene Proteins 0.000 description 1
- 102100038520 Calcitonin receptor Human genes 0.000 description 1
- 241000202252 Cerberus Species 0.000 description 1
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
- 102000003849 Cytochrome P450 Human genes 0.000 description 1
- 108091065053 DAN family Proteins 0.000 description 1
- 102000038900 DAN family Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 241000289669 Erinaceus europaeus Species 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 101150021185 FGF gene Proteins 0.000 description 1
- 101150081880 FGF1 gene Proteins 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 1
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 1
- 102100028412 Fibroblast growth factor 10 Human genes 0.000 description 1
- 102100028071 Fibroblast growth factor 7 Human genes 0.000 description 1
- 102100037680 Fibroblast growth factor 8 Human genes 0.000 description 1
- 102100037362 Fibronectin Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 102000016970 Follistatin Human genes 0.000 description 1
- 108010014612 Follistatin Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000051325 Glucagon Human genes 0.000 description 1
- 108060003199 Glucagon Proteins 0.000 description 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 102100038367 Gremlin-1 Human genes 0.000 description 1
- 229920002971 Heparan sulfate Polymers 0.000 description 1
- 102100024208 Homeobox protein MIXL1 Human genes 0.000 description 1
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 description 1
- 101000741435 Homo sapiens Calcitonin receptor Proteins 0.000 description 1
- 101000917237 Homo sapiens Fibroblast growth factor 10 Proteins 0.000 description 1
- 101001060261 Homo sapiens Fibroblast growth factor 7 Proteins 0.000 description 1
- 101001027382 Homo sapiens Fibroblast growth factor 8 Proteins 0.000 description 1
- 101001027128 Homo sapiens Fibronectin Proteins 0.000 description 1
- 101001032872 Homo sapiens Gremlin-1 Proteins 0.000 description 1
- 101001052462 Homo sapiens Homeobox protein MIXL1 Proteins 0.000 description 1
- 101000836765 Homo sapiens Protein amnionless Proteins 0.000 description 1
- 101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 description 1
- 102100023915 Insulin Human genes 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000014429 Insulin-like growth factor Human genes 0.000 description 1
- 102000008133 Iron-Binding Proteins Human genes 0.000 description 1
- 108010035210 Iron-Binding Proteins Proteins 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- 108010085895 Laminin Proteins 0.000 description 1
- 101000942966 Mus musculus Leukemia inhibitory factor Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 102000014736 Notch Human genes 0.000 description 1
- 102000005650 Notch Receptors Human genes 0.000 description 1
- 108700020479 Pancreatic hormone Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 1
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 1
- 102100033237 Pro-epidermal growth factor Human genes 0.000 description 1
- 102100027097 Protein amnionless Human genes 0.000 description 1
- 108010094028 Prothrombin Proteins 0.000 description 1
- 102100029986 Receptor tyrosine-protein kinase erbB-3 Human genes 0.000 description 1
- 101710100969 Receptor tyrosine-protein kinase erbB-3 Proteins 0.000 description 1
- 206010043276 Teratoma Diseases 0.000 description 1
- 102100026966 Thrombomodulin Human genes 0.000 description 1
- 108010079274 Thrombomodulin Proteins 0.000 description 1
- 102100021380 Transcription factor GATA-4 Human genes 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 229940050528 albumin Drugs 0.000 description 1
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 description 1
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 description 1
- 229940024142 alpha 1-antitrypsin Drugs 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000621 bronchi Anatomy 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 239000002771 cell marker Substances 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 230000001819 effect on gene Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 210000002388 eustachian tube Anatomy 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 description 1
- 229960004666 glucagon Drugs 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 210000003577 pancreatic endocrine progenitor Anatomy 0.000 description 1
- 230000000849 parathyroid Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 210000003800 pharynx Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 210000001811 primitive streak Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 102000027426 receptor tyrosine kinases Human genes 0.000 description 1
- 108091008598 receptor tyrosine kinases Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 210000001685 thyroid gland Anatomy 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/672,688 US8895300B2 (en) | 2008-11-04 | 2012-11-08 | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
| US13/672688 | 2012-11-08 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013226992A Division JP6532098B2 (ja) | 2012-11-08 | 2013-10-31 | 幹細胞集合体懸濁液組成物、その分化方法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020156084A Division JP2021007393A (ja) | 2012-11-08 | 2020-09-17 | 幹細胞集合体懸濁液組成物、その分化方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019022496A JP2019022496A (ja) | 2019-02-14 |
| JP2019022496A5 true JP2019022496A5 (enExample) | 2019-09-12 |
| JP6767445B2 JP6767445B2 (ja) | 2020-10-14 |
Family
ID=49515254
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013226992A Active JP6532098B2 (ja) | 2012-11-08 | 2013-10-31 | 幹細胞集合体懸濁液組成物、その分化方法 |
| JP2018169154A Active JP6767445B2 (ja) | 2012-11-08 | 2018-09-10 | 幹細胞集合体懸濁液組成物、その分化方法 |
| JP2020156084A Pending JP2021007393A (ja) | 2012-11-08 | 2020-09-17 | 幹細胞集合体懸濁液組成物、その分化方法 |
| JP2023025135A Active JP7580508B2 (ja) | 2012-11-08 | 2023-02-21 | 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法 |
| JP2024189513A Pending JP2025028837A (ja) | 2012-11-08 | 2024-10-29 | 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013226992A Active JP6532098B2 (ja) | 2012-11-08 | 2013-10-31 | 幹細胞集合体懸濁液組成物、その分化方法 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020156084A Pending JP2021007393A (ja) | 2012-11-08 | 2020-09-17 | 幹細胞集合体懸濁液組成物、その分化方法 |
| JP2023025135A Active JP7580508B2 (ja) | 2012-11-08 | 2023-02-21 | 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法 |
| JP2024189513A Pending JP2025028837A (ja) | 2012-11-08 | 2024-10-29 | 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法 |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP2730649B1 (enExample) |
| JP (5) | JP6532098B2 (enExample) |
| KR (6) | KR102069900B1 (enExample) |
| CN (2) | CN108342354B (enExample) |
| AU (4) | AU2013248265B2 (enExample) |
| CA (1) | CA2832194A1 (enExample) |
| DK (1) | DK2730649T3 (enExample) |
| ES (1) | ES2906102T3 (enExample) |
| IL (1) | IL229137B (enExample) |
| NZ (2) | NZ617099A (enExample) |
| SG (1) | SG2013081450A (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9944894B2 (en) * | 2015-01-16 | 2018-04-17 | General Electric Company | Pluripotent stem cell expansion and passage using a rocking platform bioreactor |
| US11242503B2 (en) | 2016-01-26 | 2022-02-08 | The Governing Council Of The University Of Toronto | Method for dissociating cell aggregates |
| IL313825B2 (en) | 2017-06-14 | 2025-07-01 | Vertex Pharma | Devices and methods for administering medications |
| WO2019124348A1 (ja) * | 2017-12-19 | 2019-06-27 | 国立大学法人京都大学 | 新規骨分化誘導方法 |
| US20210363474A1 (en) * | 2018-07-06 | 2021-11-25 | Myoridge Co. Ltd. | Method for producing cell sheet, cardiomyocyte sheet, and kit for producing cardiomyocyte sheet |
| JPWO2020022511A1 (ja) * | 2018-07-27 | 2021-08-02 | 味の素株式会社 | 動物細胞の浮遊培養用の添加物、浮遊培養用培地および浮遊培養方法 |
| WO2021118226A1 (ko) * | 2019-12-09 | 2021-06-17 | 주식회사 대웅제약 | 인간 만능줄기세포로부터 중간엽 줄기세포의 제조방법 및 이에 의해 제조된 중간엽 줄기세포 |
| JP7526460B2 (ja) * | 2020-04-03 | 2024-08-01 | 国立研究開発法人農業・食品産業技術総合研究機構 | 試料冷却方法、冷却物の製造方法及び冷却用具 |
| CN112705131B (zh) * | 2020-12-29 | 2023-01-24 | 广西中烟工业有限责任公司 | 一种低共熔溶剂/羟丙基-β-环糊精薄荷醇微囊制备方法 |
| US20220403340A1 (en) * | 2021-06-11 | 2022-12-22 | Washington University | Media for cryopreserved cells and methods of making and using same |
Family Cites Families (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2161804A (en) | 1935-11-09 | 1939-06-13 | Baldwin Southwark Corp | Hydraulic press |
| US4988623A (en) | 1988-06-30 | 1991-01-29 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Rotating bio-reactor cell culture apparatus |
| US5605822A (en) | 1989-06-15 | 1997-02-25 | The Regents Of The University Of Michigan | Methods, compositions and devices for growing human hematopoietic cells |
| US5437994A (en) | 1989-06-15 | 1995-08-01 | Regents Of The University Of Michigan | Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells |
| JPH07224089A (ja) | 1991-03-17 | 1995-08-22 | Yeda Res & Dev Co Ltd | neuレセプターの刺激因子 |
| KR950701379A (ko) | 1992-04-29 | 1995-03-23 | 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 | neu 수용체의 재조합 자극인자(RECOMBINANT STIMULATING FACTOR OF THE neu RECEPTOR) |
| US5453357A (en) | 1992-10-08 | 1995-09-26 | Vanderbilt University | Pluripotential embryonic stem cells and methods of making same |
| US5320963A (en) | 1992-11-25 | 1994-06-14 | National Research Council Of Canada | Bioreactor for the perfusion culture of cells |
| WO1994028133A1 (en) | 1993-05-21 | 1994-12-08 | Amgen Inc. | Recombinant neu differentiation factors |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| JP2001517927A (ja) | 1996-02-28 | 2001-10-09 | バンダービルト ユニバーシティ | 胚幹細胞を作製する組成物および方法 |
| US5866419A (en) | 1996-09-16 | 1999-02-02 | Meder; Martin G. | Roller bottle |
| IL130827A0 (en) | 1997-02-10 | 2001-01-28 | Genentech Inc | Heregulin variants |
| US6136558A (en) | 1997-02-10 | 2000-10-24 | Genentech, Inc. | Heregulin variants |
| US6331406B1 (en) | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
| WO1999053021A1 (en) | 1998-04-09 | 1999-10-21 | Bresagen Limited | Cell differentiation/proliferation and maintenance factor and uses thereof |
| US6667176B1 (en) * | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| EP1129176A4 (en) | 1998-11-09 | 2002-10-30 | Es Cell Int Pte Ltd | EMBRYONIC STEM CELLS |
| US7005252B1 (en) | 2000-03-09 | 2006-02-28 | Wisconsin Alumni Research Foundation | Serum free cultivation of primate embryonic stem cells |
| US6416999B1 (en) * | 2000-04-07 | 2002-07-09 | Raven Biotechnologies, Inc. | Human Müllerian duct-derived epithelial cells and methods of isolation and uses thereof |
| WO2003014313A2 (en) | 2001-08-06 | 2003-02-20 | Bresagen, Ltd. | Alternative compositions and methods for the culture of stem cells |
| US20040161419A1 (en) * | 2002-04-19 | 2004-08-19 | Strom Stephen C. | Placental stem cells and uses thereof |
| US20060030042A1 (en) | 2003-12-19 | 2006-02-09 | Ali Brivanlou | Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime |
| US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| WO2005063971A2 (en) | 2003-12-23 | 2005-07-14 | Cythera, Inc | Definitive endoderm |
| US7541185B2 (en) | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| WO2005065354A2 (en) | 2003-12-31 | 2005-07-21 | The Burnham Institute | Defined media for pluripotent stem cell culture |
| DE102004043256B4 (de) * | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension |
| US7739764B2 (en) | 2005-04-27 | 2010-06-22 | Whirlpool Corporation | Method and apparatus for monitoring load size and load imbalance in washing machine |
| DK1957636T3 (en) | 2005-10-27 | 2018-10-01 | Viacyte Inc | PDX1-EXPRESSING DORSAL AND VENTRAL FORTARM ENDODERM |
| AU2007220759A1 (en) | 2006-02-23 | 2007-09-07 | Viacyte, Inc. | Compositions and methods useful for culturing differentiable cells |
| DK2650360T3 (da) | 2006-03-02 | 2019-10-07 | Viacyte Inc | Endokrine prekursorceller, pancreatiske hormon udtrykkende celler og fremgangsmåder til fremstilling |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| WO2008013664A2 (en) | 2006-07-26 | 2008-01-31 | Cythera, Inc. | Methods of producing pancreatic hormones |
| JP2008099662A (ja) * | 2006-09-22 | 2008-05-01 | Institute Of Physical & Chemical Research | 幹細胞の培養方法 |
| US9080145B2 (en) * | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| US8211698B2 (en) * | 2007-08-02 | 2012-07-03 | California Stem Cells, Inc. | Methods of derivation of neuronal progenitor cells from embryonic stem cells |
| US7695963B2 (en) | 2007-09-24 | 2010-04-13 | Cythera, Inc. | Methods for increasing definitive endoderm production |
| US8623650B2 (en) * | 2007-10-19 | 2014-01-07 | Viacyte, Inc. | Methods and compositions for feeder-free pluripotent stem cell media containing human serum |
| CN101599496B (zh) | 2008-06-06 | 2011-06-15 | 群康科技(深圳)有限公司 | 薄膜晶体管基板与薄膜晶体管母基板 |
| US8630587B2 (en) * | 2008-07-11 | 2014-01-14 | Qualcomm Incorporated | Inter-cell interference cancellation framework |
| US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
| ES2743204T3 (es) * | 2008-11-04 | 2020-02-18 | Viacyte Inc | Composiciones de suspensión de agregados de células madre y métodos para su diferenciación |
| ES2667493T3 (es) | 2008-11-14 | 2018-05-11 | Viacyte, Inc. | Encapsulación de células pancreáticas derivadas de células madre humanas pluripotentes |
| KR20110137805A (ko) * | 2009-03-20 | 2011-12-23 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | 마이크로캐리어 상의 다분화성 및 다능성 세포의 배양 방법 |
| US20100272695A1 (en) | 2009-04-22 | 2010-10-28 | Alan Agulnick | Cell compositions derived from dedifferentiated reprogrammed cells |
| WO2012135621A2 (en) * | 2011-03-30 | 2012-10-04 | Cellular Dynamics International. Inc | Priming of pluripotent stem cells for neural differentiation |
| US10702008B2 (en) | 2018-02-26 | 2020-07-07 | Hbn Shoe, Llc | Device and method of constructing shoes |
| US11586805B2 (en) | 2021-07-26 | 2023-02-21 | Atlassian Pty Ltd. | Machine-learning-based natural language processing techniques for low-latency document summarization |
-
2013
- 2013-10-28 AU AU2013248265A patent/AU2013248265B2/en active Active
- 2013-10-29 NZ NZ617099A patent/NZ617099A/en not_active IP Right Cessation
- 2013-10-29 NZ NZ702500A patent/NZ702500A/en not_active IP Right Cessation
- 2013-10-30 IL IL229137A patent/IL229137B/en active IP Right Grant
- 2013-10-31 EP EP13191140.6A patent/EP2730649B1/en active Active
- 2013-10-31 JP JP2013226992A patent/JP6532098B2/ja active Active
- 2013-10-31 EP EP21216091.5A patent/EP4053264A1/en active Pending
- 2013-10-31 ES ES13191140T patent/ES2906102T3/es active Active
- 2013-10-31 DK DK13191140.6T patent/DK2730649T3/da active
- 2013-11-01 SG SG2013081450A patent/SG2013081450A/en unknown
- 2013-11-01 CA CA2832194A patent/CA2832194A1/en active Pending
- 2013-11-05 CN CN201810149768.3A patent/CN108342354B/zh active Active
- 2013-11-05 CN CN201310541026.2A patent/CN103805512B/zh active Active
- 2013-11-05 KR KR1020130133324A patent/KR102069900B1/ko active Active
-
2018
- 2018-09-10 JP JP2018169154A patent/JP6767445B2/ja active Active
-
2019
- 2019-01-25 AU AU2019200532A patent/AU2019200532B2/en active Active
-
2020
- 2020-01-16 KR KR1020200005917A patent/KR102180779B1/ko active Active
- 2020-09-17 JP JP2020156084A patent/JP2021007393A/ja active Pending
- 2020-11-12 KR KR1020200151217A patent/KR102357281B1/ko active Active
-
2022
- 2022-01-25 KR KR1020220010706A patent/KR102498731B1/ko active Active
- 2022-05-18 AU AU2022203343A patent/AU2022203343B2/en active Active
-
2023
- 2023-02-06 KR KR1020230015699A patent/KR102684120B1/ko active Active
- 2023-02-21 JP JP2023025135A patent/JP7580508B2/ja active Active
- 2023-08-18 AU AU2023216861A patent/AU2023216861B2/en active Active
-
2024
- 2024-07-08 KR KR1020240089546A patent/KR20240113877A/ko active Pending
- 2024-10-29 JP JP2024189513A patent/JP2025028837A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2019022496A5 (enExample) | ||
| EP2488631B1 (en) | Manipulation of osmolality for differentiating stem cells | |
| US9476030B2 (en) | Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof | |
| US8445273B2 (en) | Stem cell aggregate suspension compositions and methods of differentiation thereof | |
| JP7580508B2 (ja) | 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法 | |
| CN103354835B (zh) | 抑制人多能干细胞生长的试剂和方法 | |
| Yamanaka et al. | Pluripotency of embryonic stem cells | |
| CN104250632B (zh) | 单个多能干细胞培养 | |
| US9708582B2 (en) | Method of differentiating stem cells | |
| US20100323442A1 (en) | Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells | |
| JP2012507281A (ja) | 幹細胞集合体懸濁液組成物、その分化方法 | |
| CN101541953A (zh) | 通过从人胚胎干细胞获得的定形内胚层细胞的分化得到胰和肝内胚层细胞及组织 | |
| JP2023176013A (ja) | 幹細胞集合体懸濁液組成物、その分化方法 | |
| JP6441256B2 (ja) | 幹細胞集合体懸濁液組成物、その分化方法 | |
| JP2018148933A (ja) | 幹細胞集合体懸濁液組成物、その分化方法 | |
| HK1131634A (en) | Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems |