NZ617099A - Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof - Google Patents

Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof Download PDF

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Publication number
NZ617099A
NZ617099A NZ617099A NZ61709913A NZ617099A NZ 617099 A NZ617099 A NZ 617099A NZ 617099 A NZ617099 A NZ 617099A NZ 61709913 A NZ61709913 A NZ 61709913A NZ 617099 A NZ617099 A NZ 617099A
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NZ
New Zealand
Prior art keywords
cells
cell
culture
aggregates
roller bottle
Prior art date
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NZ617099A
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English (en)
Inventor
Thomas C Schulz
Original Assignee
Viacyte Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/672,688 external-priority patent/US8895300B2/en
Application filed by Viacyte Inc filed Critical Viacyte Inc
Priority to NZ702500A priority Critical patent/NZ702500A/en
Publication of NZ617099A publication Critical patent/NZ617099A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C12M27/12—Roller bottles; Roller tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/195—Heregulin, neu differentiation factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Professional, Industrial, Or Sporting Protective Garments (AREA)
NZ617099A 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof NZ617099A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NZ702500A NZ702500A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13/672,688 US8895300B2 (en) 2008-11-04 2012-11-08 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Publications (1)

Publication Number Publication Date
NZ617099A true NZ617099A (en) 2015-05-29

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NZ702500A NZ702500A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
NZ617099A NZ617099A (en) 2012-11-08 2013-10-29 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

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EP (2) EP4053264A1 (enExample)
JP (5) JP6532098B2 (enExample)
KR (6) KR102069900B1 (enExample)
CN (2) CN103805512B (enExample)
AU (4) AU2013248265B2 (enExample)
CA (1) CA2832194A1 (enExample)
DK (1) DK2730649T3 (enExample)
ES (1) ES2906102T3 (enExample)
IL (1) IL229137B (enExample)
NZ (2) NZ702500A (enExample)
SG (1) SG2013081450A (enExample)

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WO2019124348A1 (ja) 2017-12-19 2019-06-27 国立大学法人京都大学 新規骨分化誘導方法
US20210363474A1 (en) * 2018-07-06 2021-11-25 Myoridge Co. Ltd. Method for producing cell sheet, cardiomyocyte sheet, and kit for producing cardiomyocyte sheet
KR102853452B1 (ko) * 2018-07-27 2025-09-03 아지노모토 가부시키가이샤 동물 세포의 부유 배양용 첨가물, 부유 배양용 배지 및 부유 배양 방법
CN114787341B (zh) * 2019-12-09 2024-08-16 株式会社大熊制药 从人多能干细胞制备间充质干细胞的方法及由此制备的间充质干细胞
JP7526460B2 (ja) * 2020-04-03 2024-08-01 国立研究開発法人農業・食品産業技術総合研究機構 試料冷却方法、冷却物の製造方法及び冷却用具
CN112705131B (zh) * 2020-12-29 2023-01-24 广西中烟工业有限责任公司 一种低共熔溶剂/羟丙基-β-环糊精薄荷醇微囊制备方法
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Publication number Publication date
KR102357281B1 (ko) 2022-02-07
KR20200130798A (ko) 2020-11-20
KR102069900B1 (ko) 2020-02-11
KR102684120B1 (ko) 2024-07-12
KR20220018522A (ko) 2022-02-15
KR20200008172A (ko) 2020-01-23
EP2730649A1 (en) 2014-05-14
CN103805512A (zh) 2014-05-21
EP4053264A1 (en) 2022-09-07
EP2730649B1 (en) 2021-12-22
KR20230022928A (ko) 2023-02-16
DK2730649T3 (da) 2022-03-07
IL229137B (en) 2018-08-30
ES2906102T3 (es) 2022-04-13
KR102498731B1 (ko) 2023-02-13
JP2025028837A (ja) 2025-03-05
CA2832194A1 (en) 2014-05-08
KR102180779B1 (ko) 2020-11-19
CN108342354B (zh) 2022-03-04
AU2013248265B2 (en) 2018-11-01
SG2013081450A (en) 2014-06-27
JP2023075135A (ja) 2023-05-30
AU2022203343B2 (en) 2023-05-25
AU2022203343A1 (en) 2022-06-09
CN108342354A (zh) 2018-07-31
AU2013248265A1 (en) 2014-05-22
KR20240113877A (ko) 2024-07-23
AU2023216861A1 (en) 2023-09-07
JP6532098B2 (ja) 2019-06-19
AU2019200532A1 (en) 2019-02-14
JP2019022496A (ja) 2019-02-14
KR20140059729A (ko) 2014-05-16
AU2019200532B2 (en) 2022-02-24
CN103805512B (zh) 2018-03-27
IL229137A0 (en) 2014-03-31
JP2014094005A (ja) 2014-05-22
JP7580508B2 (ja) 2024-11-11
NZ702500A (en) 2016-07-29
JP2021007393A (ja) 2021-01-28
JP6767445B2 (ja) 2020-10-14
AU2023216861B2 (en) 2025-10-23

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