JP6532098B2 - 幹細胞集合体懸濁液組成物、その分化方法 - Google Patents

幹細胞集合体懸濁液組成物、その分化方法 Download PDF

Info

Publication number
JP6532098B2
JP6532098B2 JP2013226992A JP2013226992A JP6532098B2 JP 6532098 B2 JP6532098 B2 JP 6532098B2 JP 2013226992 A JP2013226992 A JP 2013226992A JP 2013226992 A JP2013226992 A JP 2013226992A JP 6532098 B2 JP6532098 B2 JP 6532098B2
Authority
JP
Japan
Prior art keywords
cells
cell
culture
rpm
aggregates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013226992A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014094005A5 (enExample
JP2014094005A (ja
Inventor
トーマス シー シュルツ
トーマス シー シュルツ
Original Assignee
バイアサイト インク
バイアサイト インク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/672,688 external-priority patent/US8895300B2/en
Application filed by バイアサイト インク, バイアサイト インク filed Critical バイアサイト インク
Publication of JP2014094005A publication Critical patent/JP2014094005A/ja
Publication of JP2014094005A5 publication Critical patent/JP2014094005A5/ja
Application granted granted Critical
Publication of JP6532098B2 publication Critical patent/JP6532098B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48Reproductive organs
    • A61K35/54Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
    • C12M27/12Roller bottles; Roller tubes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/195Heregulin, neu differentiation factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/235Leukemia inhibitory factor [LIF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases [EC 2.]
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Professional, Industrial, Or Sporting Protective Garments (AREA)
JP2013226992A 2012-11-08 2013-10-31 幹細胞集合体懸濁液組成物、その分化方法 Active JP6532098B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/672688 2012-11-08
US13/672,688 US8895300B2 (en) 2008-11-04 2012-11-08 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018169154A Division JP6767445B2 (ja) 2012-11-08 2018-09-10 幹細胞集合体懸濁液組成物、その分化方法

Publications (3)

Publication Number Publication Date
JP2014094005A JP2014094005A (ja) 2014-05-22
JP2014094005A5 JP2014094005A5 (enExample) 2016-12-15
JP6532098B2 true JP6532098B2 (ja) 2019-06-19

Family

ID=49515254

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2013226992A Active JP6532098B2 (ja) 2012-11-08 2013-10-31 幹細胞集合体懸濁液組成物、その分化方法
JP2018169154A Active JP6767445B2 (ja) 2012-11-08 2018-09-10 幹細胞集合体懸濁液組成物、その分化方法
JP2020156084A Pending JP2021007393A (ja) 2012-11-08 2020-09-17 幹細胞集合体懸濁液組成物、その分化方法
JP2023025135A Active JP7580508B2 (ja) 2012-11-08 2023-02-21 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法
JP2024189513A Pending JP2025028837A (ja) 2012-11-08 2024-10-29 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法

Family Applications After (4)

Application Number Title Priority Date Filing Date
JP2018169154A Active JP6767445B2 (ja) 2012-11-08 2018-09-10 幹細胞集合体懸濁液組成物、その分化方法
JP2020156084A Pending JP2021007393A (ja) 2012-11-08 2020-09-17 幹細胞集合体懸濁液組成物、その分化方法
JP2023025135A Active JP7580508B2 (ja) 2012-11-08 2023-02-21 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法
JP2024189513A Pending JP2025028837A (ja) 2012-11-08 2024-10-29 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法

Country Status (11)

Country Link
EP (2) EP2730649B1 (enExample)
JP (5) JP6532098B2 (enExample)
KR (6) KR102069900B1 (enExample)
CN (2) CN103805512B (enExample)
AU (4) AU2013248265B2 (enExample)
CA (1) CA2832194A1 (enExample)
DK (1) DK2730649T3 (enExample)
ES (1) ES2906102T3 (enExample)
IL (1) IL229137B (enExample)
NZ (2) NZ702500A (enExample)
SG (1) SG2013081450A (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
WO2017127921A1 (en) * 2016-01-26 2017-08-03 Centre For Commercialization Of Regenerative Medicine Method for dissociating cell aggregates
IL313825B2 (en) 2017-06-14 2025-07-01 Vertex Pharma Devices and methods for administering medications
JP6944207B2 (ja) * 2017-12-19 2021-10-06 国立大学法人京都大学 新規骨分化誘導方法
WO2020009188A1 (ja) * 2018-07-06 2020-01-09 株式会社マイオリッジ 細胞シートの製造方法、心筋細胞シート、および心筋細胞シート製造用キット
KR102853452B1 (ko) * 2018-07-27 2025-09-03 아지노모토 가부시키가이샤 동물 세포의 부유 배양용 첨가물, 부유 배양용 배지 및 부유 배양 방법
AU2020399869A1 (en) * 2019-12-09 2022-06-30 Daewoong Pharmaceutical Co., Ltd. Method for preparation of mesenchymal stem cell from human pluripotent stem cell and mesenchymal stem cells prepared thereby
JP7526460B2 (ja) * 2020-04-03 2024-08-01 国立研究開発法人農業・食品産業技術総合研究機構 試料冷却方法、冷却物の製造方法及び冷却用具
CN112705131B (zh) * 2020-12-29 2023-01-24 广西中烟工业有限责任公司 一种低共熔溶剂/羟丙基-β-环糊精薄荷醇微囊制备方法
US20220403340A1 (en) * 2021-06-11 2022-12-22 Washington University Media for cryopreserved cells and methods of making and using same

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2161804A (en) 1935-11-09 1939-06-13 Baldwin Southwark Corp Hydraulic press
US4988623A (en) 1988-06-30 1991-01-29 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Rotating bio-reactor cell culture apparatus
US5437994A (en) 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5605822A (en) 1989-06-15 1997-02-25 The Regents Of The University Of Michigan Methods, compositions and devices for growing human hematopoietic cells
NZ242000A (en) 1991-03-17 1994-03-25 Yeda Res & Dev Purified mammalian proteinaceous factor which stimulates neu receptor
MX9302475A (es) 1992-04-29 1994-05-31 Amgen Inc Polipeptido de origen no natural que tiene la actividad del factor recombinante de estimulacion del receptor neu y proceso para la produccion del mis mo.
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5320963A (en) 1992-11-25 1994-06-14 National Research Council Of Canada Bioreactor for the perfusion culture of cells
AU7042994A (en) 1993-05-21 1994-12-20 Amgen, Inc. Recombinant (neu) differentiation factors
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU2192997A (en) 1996-02-28 1997-09-16 Vanderbilt University Compositions and methods of making embryonic stem cells
US5866419A (en) 1996-09-16 1999-02-02 Meder; Martin G. Roller bottle
IL130827A0 (en) 1997-02-10 2001-01-28 Genentech Inc Heregulin variants
US6136558A (en) 1997-02-10 2000-10-24 Genentech, Inc. Heregulin variants
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
CA2324591A1 (en) 1998-04-09 1999-10-21 Bresagen Limited Cell differentiation/proliferation and maintenance factor and uses thereof
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
WO2000027995A1 (en) 1998-11-09 2000-05-18 Monash University Embryonic stem cells
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6416999B1 (en) * 2000-04-07 2002-07-09 Raven Biotechnologies, Inc. Human Müllerian duct-derived epithelial cells and methods of isolation and uses thereof
US7432104B2 (en) 2001-08-06 2008-10-07 Bresgen Inc. Methods for the culture of human embryonic stem cells on human feeder cells
US20040161419A1 (en) * 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
US20060030042A1 (en) 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
WO2005063971A2 (en) 2003-12-23 2005-07-14 Cythera, Inc Definitive endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
US7739764B2 (en) 2005-04-27 2010-06-22 Whirlpool Corporation Method and apparatus for monitoring load size and load imbalance in washing machine
CN101351547A (zh) 2005-10-27 2009-01-21 赛瑟拉公司 表达pdx1的背侧和腹侧前肠内胚层
CN113088483A (zh) 2006-02-23 2021-07-09 维亚赛特公司 用于培养可分化细胞的组合物和方法
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
DK2650360T3 (da) 2006-03-02 2019-10-07 Viacyte Inc Endokrine prekursorceller, pancreatiske hormon udtrykkende celler og fremgangsmåder til fremstilling
WO2008013664A2 (en) 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
US9080145B2 (en) * 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
EP2537522A1 (en) * 2007-08-02 2012-12-26 California Stem Cell, Inc. Neuronal progenitor cells and methods of derivation and purification of neuronal progenitor cells from embryonic stem cells
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8623650B2 (en) * 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
CN101599496B (zh) 2008-06-06 2011-06-15 群康科技(深圳)有限公司 薄膜晶体管基板与薄膜晶体管母基板
US8630587B2 (en) 2008-07-11 2014-01-14 Qualcomm Incorporated Inter-cell interference cancellation framework
CN102361970B (zh) * 2008-11-04 2018-03-27 韦尔赛特公司 干细胞聚集体悬浮组合物及其分化方法
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
PL2356227T3 (pl) 2008-11-14 2018-07-31 Viacyte, Inc. Kapsułkowanie komórek trzustki pochodzących z ludzkich pluripotencjalnych komórek macierzystych
US9150829B2 (en) * 2009-03-20 2015-10-06 Agency For Science, Technoloy And Research Culture of pluripotent and multipotent cells on microcarriers
ES2849181T3 (es) 2009-04-22 2021-08-16 Viacyte Inc Composiciones celulares derivadas de células reprogramadas desdiferenciadas
US9487752B2 (en) * 2011-03-30 2016-11-08 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
US10702008B2 (en) 2018-02-26 2020-07-07 Hbn Shoe, Llc Device and method of constructing shoes
US11586805B2 (en) 2021-07-26 2023-02-21 Atlassian Pty Ltd. Machine-learning-based natural language processing techniques for low-latency document summarization

Also Published As

Publication number Publication date
JP2023075135A (ja) 2023-05-30
AU2023216861B2 (en) 2025-10-23
AU2013248265A1 (en) 2014-05-22
NZ617099A (en) 2015-05-29
JP2021007393A (ja) 2021-01-28
KR20200130798A (ko) 2020-11-20
KR102180779B1 (ko) 2020-11-19
EP2730649A1 (en) 2014-05-14
KR102357281B1 (ko) 2022-02-07
CN103805512A (zh) 2014-05-21
KR20220018522A (ko) 2022-02-15
JP6767445B2 (ja) 2020-10-14
KR20240113877A (ko) 2024-07-23
CA2832194A1 (en) 2014-05-08
SG2013081450A (en) 2014-06-27
KR102069900B1 (ko) 2020-02-11
KR102684120B1 (ko) 2024-07-12
AU2013248265B2 (en) 2018-11-01
CN108342354B (zh) 2022-03-04
JP2025028837A (ja) 2025-03-05
CN108342354A (zh) 2018-07-31
JP7580508B2 (ja) 2024-11-11
AU2019200532A1 (en) 2019-02-14
IL229137A0 (en) 2014-03-31
AU2022203343A1 (en) 2022-06-09
KR20230022928A (ko) 2023-02-16
NZ702500A (en) 2016-07-29
EP4053264A1 (en) 2022-09-07
AU2022203343B2 (en) 2023-05-25
CN103805512B (zh) 2018-03-27
KR102498731B1 (ko) 2023-02-13
JP2019022496A (ja) 2019-02-14
AU2019200532B2 (en) 2022-02-24
EP2730649B1 (en) 2021-12-22
KR20200008172A (ko) 2020-01-23
AU2023216861A1 (en) 2023-09-07
JP2014094005A (ja) 2014-05-22
KR20140059729A (ko) 2014-05-16
DK2730649T3 (da) 2022-03-07
IL229137B (en) 2018-08-30
ES2906102T3 (es) 2022-04-13

Similar Documents

Publication Publication Date Title
US12234477B2 (en) Scalable primate pluripotent stem cell aggregate suspension culture
JP7580508B2 (ja) 霊長類多能性幹細胞集合体懸濁培養物及びその分化方法
JP5390624B2 (ja) 幹細胞集合体懸濁液組成物、その分化方法
US8008075B2 (en) Stem cell aggregate suspension compositions and methods of differentiation thereof
JP6810103B2 (ja) 幹細胞集合体懸濁液組成物、その分化方法
JP6441256B2 (ja) 幹細胞集合体懸濁液組成物、その分化方法
JP2023176013A (ja) 幹細胞集合体懸濁液組成物、その分化方法
HK40080876A (en) Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
JP2014036660A (ja) 幹細胞集合体懸濁液組成物、その分化方法
HK1191376A (en) Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161024

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20161024

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161025

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170828

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171127

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20180406

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20180410

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20180508

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180910

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20181029

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190108

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190404

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190423

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190516

R150 Certificate of patent or registration of utility model

Ref document number: 6532098

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250