ES2906102T3 - Cultivo escalable en suspensión de agregados de células madre pluripotentes de primate y su diferenciación - Google Patents

Cultivo escalable en suspensión de agregados de células madre pluripotentes de primate y su diferenciación Download PDF

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Publication number
ES2906102T3
ES2906102T3 ES13191140T ES13191140T ES2906102T3 ES 2906102 T3 ES2906102 T3 ES 2906102T3 ES 13191140 T ES13191140 T ES 13191140T ES 13191140 T ES13191140 T ES 13191140T ES 2906102 T3 ES2906102 T3 ES 2906102T3
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cells
cell
culture
aggregates
rpm
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Spanish (es)
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Thomas Schulz
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Viacyte Inc
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Viacyte Inc
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48Reproductive organs
    • A61K35/54Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0696Artificially induced pluripotent stem cells, e.g. iPS
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    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
    • C12M27/12Roller bottles; Roller tubes
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/195Heregulin, neu differentiation factor
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/235Leukemia inhibitory factor [LIF]
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
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    • C12N2501/727Kinases (EC 2.7.)
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
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    • C12N2527/00Culture process characterised by the use of mechanical forces, e.g. strain, vibration
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    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Developmental Biology & Embryology (AREA)
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  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
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  • Transplantation (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Immunology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Professional, Industrial, Or Sporting Protective Garments (AREA)
ES13191140T 2012-11-08 2013-10-31 Cultivo escalable en suspensión de agregados de células madre pluripotentes de primate y su diferenciación Active ES2906102T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13/672,688 US8895300B2 (en) 2008-11-04 2012-11-08 Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

Publications (1)

Publication Number Publication Date
ES2906102T3 true ES2906102T3 (es) 2022-04-13

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EP (2) EP2730649B1 (enExample)
JP (5) JP6532098B2 (enExample)
KR (6) KR102069900B1 (enExample)
CN (2) CN103805512B (enExample)
AU (4) AU2013248265B2 (enExample)
CA (1) CA2832194A1 (enExample)
DK (1) DK2730649T3 (enExample)
ES (1) ES2906102T3 (enExample)
IL (1) IL229137B (enExample)
NZ (2) NZ617099A (enExample)
SG (1) SG2013081450A (enExample)

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CN112272699A (zh) * 2018-07-06 2021-01-26 株式会社迈傲锐治 细胞片的制造方法、心肌细胞片和用于制造心肌细胞片的试剂盒
KR102853452B1 (ko) * 2018-07-27 2025-09-03 아지노모토 가부시키가이샤 동물 세포의 부유 배양용 첨가물, 부유 배양용 배지 및 부유 배양 방법
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JP7580508B2 (ja) 2024-11-11
KR20240113877A (ko) 2024-07-23
AU2022203343B2 (en) 2023-05-25
KR102684120B1 (ko) 2024-07-12
EP4053264A1 (en) 2022-09-07
CN103805512B (zh) 2018-03-27
SG2013081450A (en) 2014-06-27
KR20140059729A (ko) 2014-05-16
NZ617099A (en) 2015-05-29
KR20230022928A (ko) 2023-02-16
CN108342354A (zh) 2018-07-31
KR102069900B1 (ko) 2020-02-11
KR20200130798A (ko) 2020-11-20
KR20220018522A (ko) 2022-02-15
JP2023075135A (ja) 2023-05-30
JP2019022496A (ja) 2019-02-14
AU2023216861B2 (en) 2025-10-23
JP6532098B2 (ja) 2019-06-19
JP6767445B2 (ja) 2020-10-14
JP2025028837A (ja) 2025-03-05
EP2730649B1 (en) 2021-12-22
KR102180779B1 (ko) 2020-11-19
DK2730649T3 (da) 2022-03-07
IL229137B (en) 2018-08-30
AU2019200532B2 (en) 2022-02-24
AU2013248265B2 (en) 2018-11-01
AU2013248265A1 (en) 2014-05-22
CA2832194A1 (en) 2014-05-08
CN103805512A (zh) 2014-05-21
EP2730649A1 (en) 2014-05-14
JP2014094005A (ja) 2014-05-22
KR102357281B1 (ko) 2022-02-07
KR20200008172A (ko) 2020-01-23
AU2023216861A1 (en) 2023-09-07
IL229137A0 (en) 2014-03-31
KR102498731B1 (ko) 2023-02-13
NZ702500A (en) 2016-07-29
AU2022203343A1 (en) 2022-06-09
JP2021007393A (ja) 2021-01-28
AU2019200532A1 (en) 2019-02-14
CN108342354B (zh) 2022-03-04

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