JP2018520639A5 - - Google Patents
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- JP2018520639A5 JP2018520639A5 JP2017556123A JP2017556123A JP2018520639A5 JP 2018520639 A5 JP2018520639 A5 JP 2018520639A5 JP 2017556123 A JP2017556123 A JP 2017556123A JP 2017556123 A JP2017556123 A JP 2017556123A JP 2018520639 A5 JP2018520639 A5 JP 2018520639A5
- Authority
- JP
- Japan
- Prior art keywords
- til
- tcr
- polynucleotide
- cell
- item
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 258
- 108091033319 polynucleotide Proteins 0.000 claims description 254
- 102000040430 polynucleotide Human genes 0.000 claims description 254
- 239000002157 polynucleotide Substances 0.000 claims description 254
- 210000003171 tumor-infiltrating lymphocyte Anatomy 0.000 claims description 172
- 229920001184 polypeptide Polymers 0.000 claims description 130
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 130
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 130
- 239000012472 biological sample Substances 0.000 claims description 80
- 210000004027 cell Anatomy 0.000 claims description 76
- 201000010099 disease Diseases 0.000 claims description 45
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 45
- 238000012163 sequencing technique Methods 0.000 claims description 38
- 230000000295 complement effect Effects 0.000 claims description 31
- 239000000427 antigen Substances 0.000 claims description 21
- 108091007433 antigens Proteins 0.000 claims description 21
- 102000036639 antigens Human genes 0.000 claims description 21
- 239000007787 solid Substances 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 13
- 210000004698 lymphocyte Anatomy 0.000 claims description 13
- 210000004602 germ cell Anatomy 0.000 claims description 12
- 239000003112 inhibitor Substances 0.000 claims description 12
- 239000000523 sample Substances 0.000 claims description 10
- 238000012216 screening Methods 0.000 claims description 10
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 claims description 8
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 8
- 206010028980 Neoplasm Diseases 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 6
- 201000011510 cancer Diseases 0.000 claims description 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 6
- 239000002773 nucleotide Substances 0.000 claims description 6
- 125000003729 nucleotide group Chemical group 0.000 claims description 6
- 230000035772 mutation Effects 0.000 claims description 5
- 108010026552 Proteome Proteins 0.000 claims description 3
- 108020004459 Small interfering RNA Proteins 0.000 claims description 3
- 230000031146 intracellular signal transduction Effects 0.000 claims description 3
- 238000003498 protein array Methods 0.000 claims description 3
- 208000023275 Autoimmune disease Diseases 0.000 claims description 2
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 2
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims description 2
- 102100034343 Integrase Human genes 0.000 claims description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 210000002919 epithelial cell Anatomy 0.000 claims description 2
- 238000001502 gel electrophoresis Methods 0.000 claims description 2
- 238000004949 mass spectrometry Methods 0.000 claims description 2
- 238000000159 protein binding assay Methods 0.000 claims description 2
- 238000001086 yeast two-hybrid system Methods 0.000 claims description 2
- 108060003951 Immunoglobulin Proteins 0.000 claims 3
- 108091008874 T cell receptors Proteins 0.000 claims 3
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims 3
- 102000018358 immunoglobulin Human genes 0.000 claims 3
- 231100000023 Cell-mediated cytotoxicity Toxicity 0.000 claims 1
- 206010057250 Cell-mediated cytotoxicity Diseases 0.000 claims 1
- 108091026890 Coding region Proteins 0.000 claims 1
- 238000007622 bioinformatic analysis Methods 0.000 claims 1
- 230000005890 cell-mediated cytotoxicity Effects 0.000 claims 1
- 238000002784 cytotoxicity assay Methods 0.000 claims 1
- 230000003053 immunization Effects 0.000 claims 1
- 238000002649 immunization Methods 0.000 claims 1
- 230000004068 intracellular signaling Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 239000012491 analyte Substances 0.000 description 16
- 102000039446 nucleic acids Human genes 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 5
- 239000012634 fragment Substances 0.000 description 4
- 239000013598 vector Substances 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000002702 ribosome display Methods 0.000 description 2
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000003766 bioinformatics method Methods 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003384 small molecules Chemical group 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021093583A JP2021129601A (ja) | 2015-04-27 | 2021-06-03 | 治療用薬剤および疾患特異的抗原をシーケンシング、判定、ペアリングおよび検証する方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562153041P | 2015-04-27 | 2015-04-27 | |
| US62/153,041 | 2015-04-27 | ||
| PCT/US2016/029556 WO2016176322A1 (en) | 2015-04-27 | 2016-04-27 | Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021093583A Division JP2021129601A (ja) | 2015-04-27 | 2021-06-03 | 治療用薬剤および疾患特異的抗原をシーケンシング、判定、ペアリングおよび検証する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018520639A JP2018520639A (ja) | 2018-08-02 |
| JP2018520639A5 true JP2018520639A5 (enExample) | 2019-06-06 |
| JP6894380B2 JP6894380B2 (ja) | 2021-06-30 |
Family
ID=57199815
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017556123A Expired - Fee Related JP6894380B2 (ja) | 2015-04-27 | 2016-04-27 | 治療用薬剤および疾患特異的抗原をシーケンシング、判定、ペアリングおよび検証する方法 |
| JP2021093583A Pending JP2021129601A (ja) | 2015-04-27 | 2021-06-03 | 治療用薬剤および疾患特異的抗原をシーケンシング、判定、ペアリングおよび検証する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021093583A Pending JP2021129601A (ja) | 2015-04-27 | 2021-06-03 | 治療用薬剤および疾患特異的抗原をシーケンシング、判定、ペアリングおよび検証する方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US10982258B2 (enExample) |
| EP (1) | EP3289082A4 (enExample) |
| JP (2) | JP6894380B2 (enExample) |
| CN (1) | CN107849560B (enExample) |
| AU (1) | AU2016253964B2 (enExample) |
| CA (1) | CA2983937A1 (enExample) |
| HK (1) | HK1252248A1 (enExample) |
| MX (1) | MX2017013471A (enExample) |
| WO (1) | WO2016176322A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2015188839A2 (en) | 2014-06-13 | 2015-12-17 | Immudex Aps | General detection and isolation of specific cells by binding of labeled molecules |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| PH12018500645B1 (en) | 2015-09-24 | 2022-10-21 | Abvitro Llc | Hiv antibody compositions and methods of use |
| AU2016326734B2 (en) * | 2015-09-25 | 2022-07-07 | Abvitro Llc | High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| WO2017214557A1 (en) * | 2016-06-10 | 2017-12-14 | Counsyl, Inc. | Nucleic acid sequencing adapters and uses thereof |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| MY201744A (en) | 2016-12-23 | 2024-03-15 | Visterra Inc | Binding polypeptides and methods of making the same |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| CN111148849A (zh) | 2017-05-26 | 2020-05-12 | 阿布维托有限责任公司 | 高通量多核苷酸文库测序和转录组分析 |
| KR20250096881A (ko) | 2017-08-09 | 2025-06-27 | 주노 쎄러퓨티크스 인코퍼레이티드 | 유전자 조작된 세포를 제조하기 위한 방법 및 조성물 |
| US20200292526A1 (en) | 2017-09-07 | 2020-09-17 | Juno Therapeutics, Inc. | Methods of identifying cellular attributes related to outcomes associated with cell therapy |
| WO2019084538A1 (en) * | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| WO2019118937A1 (en) | 2017-12-15 | 2019-06-20 | Juno Therapeutics, Inc. | Anti-cct5 binding molecules and methods of use thereof |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| WO2019241249A1 (en) * | 2018-06-11 | 2019-12-19 | The Brigham And Women's Hospital, Inc. | Single cell cloning approaches for biological studies |
| JP7145980B2 (ja) | 2018-06-15 | 2022-10-03 | エフ．ホフマン－ラ ロシュ アーゲー | 腫瘍浸潤リンパ球にて発現するｔ細胞受容体により認識される抗原を識別するためのシステム |
| CN116144706A (zh) | 2018-08-13 | 2023-05-23 | 根路径基因组学公司 | 成对的二分免疫受体多核苷酸的高通量克隆及其应用 |
| EP3867406B1 (en) * | 2018-10-19 | 2023-01-04 | Akoya Biosciences, Inc. | Detection of co-occurring receptor-coding nucleic acid segments |
| MX2021009087A (es) | 2019-01-29 | 2021-09-08 | Juno Therapeutics Inc | Anticuerpos y receptores quimericos de antigenos especificos para receptor 1 huerfano tipo receptor tirosina-cinasa (ror1). |
| AU2020253491A1 (en) | 2019-04-05 | 2021-10-28 | Rootpath Genomics, Inc. | Compositions and methods for T-cell receptor gene assembly |
| CA3157605A1 (en) * | 2019-11-13 | 2021-05-20 | Volker Schellenberger | Barcoded xten polypeptides and compositions thereof, and methods for making and using the same |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| CN111218502B (zh) * | 2020-04-23 | 2020-07-21 | 圣湘生物科技股份有限公司 | 提升qPCR检测性能的组合物、反应液、用途及方法 |
| JP7727662B2 (ja) | 2020-05-13 | 2025-08-21 | ジュノー セラピューティクス インコーポレイテッド | 臨床応答に関連する特徴量の特定方法およびその使用 |
| WO2022081643A2 (en) * | 2020-10-13 | 2022-04-21 | 10X Genomics, Inc. | Compositions and methods for generating recombinant antigen binding molecules from single cells |
| CN114464253B (zh) * | 2022-03-03 | 2023-03-10 | 予果生物科技（北京）有限公司 | 基于长读长测序进行实时病原检测的方法、系统和应用 |
| US20250295696A1 (en) * | 2022-05-03 | 2025-09-25 | 3T Biosciences, Inc. | T-cell target discovery |
| WO2024015862A1 (en) * | 2022-07-13 | 2024-01-18 | 10X Genomics, Inc. | Methods for characterization of antigen-binding molecules from biological samples |
| WO2024192051A1 (en) | 2023-03-13 | 2024-09-19 | Turnstone Biologics Corp. | Composition of selected tumor infiltrating lymphocytes and related methods of producing and using the same |
| GB2629157A (en) * | 2023-04-18 | 2024-10-23 | The Institute Of Cancer Res Royal Cancer Hospital | T cell receptor sequencing |
| WO2025080865A1 (en) | 2023-10-11 | 2025-04-17 | Turnstone Biologics Corp. | Combination of tumor infiltrating lymphocytes (til) and low dose radiation |
| WO2025096638A2 (en) | 2023-10-30 | 2025-05-08 | Turnstone Biologics Corp. | Genetically modified tumor infilitrating lymphocytes and methods of producing and using the same |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990004648A1 (en) | 1988-10-20 | 1990-05-03 | Alexander Alan Morley | Method for diagnosis of monoclonality in leukaemia and lymphoma |
| US20070160994A1 (en) * | 2003-12-15 | 2007-07-12 | Institut Pasteur | Repertoire determination of a lymphocyte b population |
| US8268564B2 (en) * | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| GB2483810B (en) | 2008-11-07 | 2012-09-05 | Sequenta Inc | Methods for correlating clonotypes with diseases in a population |
| WO2011037990A1 (en) * | 2009-09-22 | 2011-03-31 | President And Fellows Of Harvard College | Entangled mate sequencing |
| EP2670863B1 (en) * | 2011-01-31 | 2018-06-27 | H. Hoffnabb-La Roche Ag | Methods of identifying multiple epitopes in cells |
| EP3421591B1 (en) * | 2011-04-28 | 2023-09-27 | The Board of Trustees of the Leland Stanford Junior University | Identification of polynucleotides associated with a sample |
| SI2755997T1 (sl) * | 2011-09-15 | 2018-11-30 | The United States of America, as represented by Secretary, Department of Health and Human Services, Office of Technology Transfer, National Institutes of Health | T-celični receptorji, ki prepoznajo HLA-A1- ali HLA-CW7-omejeni mage |
| EP2768982A4 (en) | 2011-10-21 | 2015-06-03 | Adaptive Biotechnologies Corp | QUANTIFICATION OF ADAPTIVE IMMUNOCELL GENOMES IN A COMPLEX MIX OF CELLS |
| US9708654B2 (en) * | 2012-06-15 | 2017-07-18 | Board Of Regents, The University Of Texas System | High throughput sequencing of multiple transcripts |
| WO2014130685A1 (en) * | 2013-02-22 | 2014-08-28 | Sequenta, Inc. | Rare clonotypes and uses thereof |
| US10119134B2 (en) * | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| SG11201506991VA (en) * | 2013-03-15 | 2015-10-29 | Adaptive Biotechnologies Corp | Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set |
| CN110964796B (zh) | 2013-08-28 | 2024-04-05 | 贝克顿迪金森公司 | 大规模平行单细胞分析 |
| EP3626748A1 (en) * | 2014-01-13 | 2020-03-25 | Stephen J. Forman | Chimeric antigen receptors (cars) having mutations in the fc spacer region and methods for their use |
| SG10201911069WA (en) | 2014-09-15 | 2020-01-30 | Abvitro Llc | High-throughput nucleotide library sequencing |
| US11111519B2 (en) * | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
-
2016
- 2016-04-27 CA CA2983937A patent/CA2983937A1/en active Pending
- 2016-04-27 HK HK18111487.7A patent/HK1252248A1/zh unknown
- 2016-04-27 US US15/570,075 patent/US10982258B2/en not_active Expired - Fee Related
- 2016-04-27 AU AU2016253964A patent/AU2016253964B2/en not_active Ceased
- 2016-04-27 MX MX2017013471A patent/MX2017013471A/es unknown
- 2016-04-27 EP EP16787061.7A patent/EP3289082A4/en not_active Withdrawn
- 2016-04-27 CN CN201680039185.4A patent/CN107849560B/zh not_active Expired - Fee Related
- 2016-04-27 JP JP2017556123A patent/JP6894380B2/ja not_active Expired - Fee Related
- 2016-04-27 WO PCT/US2016/029556 patent/WO2016176322A1/en not_active Ceased
-
2020
- 2020-08-31 US US17/008,535 patent/US20210079454A1/en not_active Abandoned
-
2021
- 2021-06-03 JP JP2021093583A patent/JP2021129601A/ja active Pending
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