CN107849560B - 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法 - Google Patents

对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法 Download PDF

Info

Publication number
CN107849560B
CN107849560B CN201680039185.4A CN201680039185A CN107849560B CN 107849560 B CN107849560 B CN 107849560B CN 201680039185 A CN201680039185 A CN 201680039185A CN 107849560 B CN107849560 B CN 107849560B
Authority
CN
China
Prior art keywords
tcr
polynucleotide
polypeptide
sequence
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201680039185.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN107849560A (zh
Inventor
弗朗索瓦·维格纳尔特
阿德里安·阮格汉姆·布里格斯
克里斯多佛·赖安·克劳泽
斯蒂芬·雅各布·戈德弗莱斯
索尼亚·汀布莱克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abvitro LLC
Original Assignee
Abvitro LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abvitro LLC filed Critical Abvitro LLC
Publication of CN107849560A publication Critical patent/CN107849560A/zh
Application granted granted Critical
Publication of CN107849560B publication Critical patent/CN107849560B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/06Immunosuppressants, e.g. drugs for graft rejection
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503Immunoglobulin superfamily
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Plant Pathology (AREA)
  • Transplantation (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201680039185.4A 2015-04-27 2016-04-27 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法 Expired - Fee Related CN107849560B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562153041P 2015-04-27 2015-04-27
US62/153,041 2015-04-27
PCT/US2016/029556 WO2016176322A1 (en) 2015-04-27 2016-04-27 Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens

Publications (2)

Publication Number Publication Date
CN107849560A CN107849560A (zh) 2018-03-27
CN107849560B true CN107849560B (zh) 2021-10-22

Family

ID=57199815

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680039185.4A Expired - Fee Related CN107849560B (zh) 2015-04-27 2016-04-27 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法

Country Status (9)

Country Link
US (2) US10982258B2 (enExample)
EP (1) EP3289082A4 (enExample)
JP (2) JP6894380B2 (enExample)
CN (1) CN107849560B (enExample)
AU (1) AU2016253964B2 (enExample)
CA (1) CA2983937A1 (enExample)
HK (1) HK1252248A1 (enExample)
MX (1) MX2017013471A (enExample)
WO (1) WO2016176322A1 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2015188839A2 (en) 2014-06-13 2015-12-17 Immudex Aps General detection and isolation of specific cells by binding of labeled molecules
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
PH12018500645B1 (en) 2015-09-24 2022-10-21 Abvitro Llc Hiv antibody compositions and methods of use
AU2016326734B2 (en) * 2015-09-25 2022-07-07 Abvitro Llc High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017214557A1 (en) * 2016-06-10 2017-12-14 Counsyl, Inc. Nucleic acid sequencing adapters and uses thereof
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
MY201744A (en) 2016-12-23 2024-03-15 Visterra Inc Binding polypeptides and methods of making the same
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN111148849A (zh) 2017-05-26 2020-05-12 阿布维托有限责任公司 高通量多核苷酸文库测序和转录组分析
KR20250096881A (ko) 2017-08-09 2025-06-27 주노 쎄러퓨티크스 인코퍼레이티드 유전자 조작된 세포를 제조하기 위한 방법 및 조성물
US20200292526A1 (en) 2017-09-07 2020-09-17 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
WO2019084538A1 (en) * 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
WO2019118937A1 (en) 2017-12-15 2019-06-20 Juno Therapeutics, Inc. Anti-cct5 binding molecules and methods of use thereof
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2019241249A1 (en) * 2018-06-11 2019-12-19 The Brigham And Women's Hospital, Inc. Single cell cloning approaches for biological studies
JP7145980B2 (ja) 2018-06-15 2022-10-03 エフ.ホフマン-ラ ロシュ アーゲー 腫瘍浸潤リンパ球にて発現するt細胞受容体により認識される抗原を識別するためのシステム
CN116144706A (zh) 2018-08-13 2023-05-23 根路径基因组学公司 成对的二分免疫受体多核苷酸的高通量克隆及其应用
EP3867406B1 (en) * 2018-10-19 2023-01-04 Akoya Biosciences, Inc. Detection of co-occurring receptor-coding nucleic acid segments
MX2021009087A (es) 2019-01-29 2021-09-08 Juno Therapeutics Inc Anticuerpos y receptores quimericos de antigenos especificos para receptor 1 huerfano tipo receptor tirosina-cinasa (ror1).
AU2020253491A1 (en) 2019-04-05 2021-10-28 Rootpath Genomics, Inc. Compositions and methods for T-cell receptor gene assembly
CA3157605A1 (en) * 2019-11-13 2021-05-20 Volker Schellenberger Barcoded xten polypeptides and compositions thereof, and methods for making and using the same
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
CN111218502B (zh) * 2020-04-23 2020-07-21 圣湘生物科技股份有限公司 提升qPCR检测性能的组合物、反应液、用途及方法
JP7727662B2 (ja) 2020-05-13 2025-08-21 ジュノー セラピューティクス インコーポレイテッド 臨床応答に関連する特徴量の特定方法およびその使用
WO2022081643A2 (en) * 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
CN114464253B (zh) * 2022-03-03 2023-03-10 予果生物科技(北京)有限公司 基于长读长测序进行实时病原检测的方法、系统和应用
US20250295696A1 (en) * 2022-05-03 2025-09-25 3T Biosciences, Inc. T-cell target discovery
WO2024015862A1 (en) * 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024192051A1 (en) 2023-03-13 2024-09-19 Turnstone Biologics Corp. Composition of selected tumor infiltrating lymphocytes and related methods of producing and using the same
GB2629157A (en) * 2023-04-18 2024-10-23 The Institute Of Cancer Res Royal Cancer Hospital T cell receptor sequencing
WO2025080865A1 (en) 2023-10-11 2025-04-17 Turnstone Biologics Corp. Combination of tumor infiltrating lymphocytes (til) and low dose radiation
WO2025096638A2 (en) 2023-10-30 2025-05-08 Turnstone Biologics Corp. Genetically modified tumor infilitrating lymphocytes and methods of producing and using the same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103703143A (zh) * 2011-01-31 2014-04-02 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
CN103732738A (zh) * 2011-04-28 2014-04-16 小利兰斯坦福大学托管委员会 与样品相关的多核苷酸的鉴定
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
WO2014145992A1 (en) * 2013-03-15 2014-09-18 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990004648A1 (en) 1988-10-20 1990-05-03 Alexander Alan Morley Method for diagnosis of monoclonality in leukaemia and lymphoma
US20070160994A1 (en) * 2003-12-15 2007-07-12 Institut Pasteur Repertoire determination of a lymphocyte b population
US8268564B2 (en) * 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
GB2483810B (en) 2008-11-07 2012-09-05 Sequenta Inc Methods for correlating clonotypes with diseases in a population
WO2011037990A1 (en) * 2009-09-22 2011-03-31 President And Fellows Of Harvard College Entangled mate sequencing
SI2755997T1 (sl) * 2011-09-15 2018-11-30 The United States of America, as represented by Secretary, Department of Health and Human Services, Office of Technology Transfer, National Institutes of Health T-celični receptorji, ki prepoznajo HLA-A1- ali HLA-CW7-omejeni mage
EP2768982A4 (en) 2011-10-21 2015-06-03 Adaptive Biotechnologies Corp QUANTIFICATION OF ADAPTIVE IMMUNOCELL GENOMES IN A COMPLEX MIX OF CELLS
US9708654B2 (en) * 2012-06-15 2017-07-18 Board Of Regents, The University Of Texas System High throughput sequencing of multiple transcripts
WO2014130685A1 (en) * 2013-02-22 2014-08-28 Sequenta, Inc. Rare clonotypes and uses thereof
CN110964796B (zh) 2013-08-28 2024-04-05 贝克顿迪金森公司 大规模平行单细胞分析
EP3626748A1 (en) * 2014-01-13 2020-03-25 Stephen J. Forman Chimeric antigen receptors (cars) having mutations in the fc spacer region and methods for their use
SG10201911069WA (en) 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
US11111519B2 (en) * 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103703143A (zh) * 2011-01-31 2014-04-02 爱普瑞斯生物公司 鉴定细胞中的多个表位的方法
CN103732738A (zh) * 2011-04-28 2014-04-16 小利兰斯坦福大学托管委员会 与样品相关的多核苷酸的鉴定
WO2014144495A1 (en) * 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
WO2014145992A1 (en) * 2013-03-15 2014-09-18 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
The promise and challenge of high-throughput sequencing of the antibody repertoire;George Georgiou等;《Nature Biotechnology》;20140119;第158-168页 *
高通量DNA测序技术在抗体新药研发中的应用;余馨等;《药学学报》;20121231;第322-331页 *

Also Published As

Publication number Publication date
WO2016176322A1 (en) 2016-11-03
AU2016253964B2 (en) 2022-07-07
EP3289082A1 (en) 2018-03-07
JP6894380B2 (ja) 2021-06-30
CA2983937A1 (en) 2016-11-03
JP2021129601A (ja) 2021-09-09
US20190024145A1 (en) 2019-01-24
EP3289082A4 (en) 2018-09-12
US20210079454A1 (en) 2021-03-18
JP2018520639A (ja) 2018-08-02
HK1252248A1 (zh) 2019-05-24
MX2017013471A (es) 2018-08-01
US10982258B2 (en) 2021-04-20
AU2016253964A1 (en) 2017-11-09
CN107849560A (zh) 2018-03-27

Similar Documents

Publication Publication Date Title
CN107849560B (zh) 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
US20210293812A1 (en) High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
JP5498018B2 (ja) 胎盤成長因子(PlGF)媒介性の転移および/または血管新生の阻害
JP6869233B2 (ja) 親和性−オリゴヌクレオチドコンジュゲートおよびその使用
CN116096749A (zh) Ccr8抗体及其用途
US11655303B2 (en) Anti-CD39 antibody compositions and methods
CN115551895A (zh) 抗ccr8剂
US20170269092A1 (en) Anti-mutant calreticulin antibodies and their use in the diagnosis and therapy of myeloid malignancies
CN104684931B (zh) 抗cd26抗体及其用途
WO2022187050A1 (en) Anti-cd117 agents and compositions and methods for making and using the same
TW202342541A (zh) 單域抗Nectin-4抗體
TW202204406A (zh) 抗個體遺傳型抗體及使用其之方法
HK40091263A (zh) 用於治疗应用的ccr8抗体
HK40090308A (zh) 抗ccr8抗体疗法:生物标志物和组合疗法
RU2778754C2 (ru) Конъюгаты аффинная часть-олигонуклеотид и их применения
WO2025072313A1 (en) Anti-gpc4 antibodies
KR20240119326A (ko) 종양 마이크로바이옴을 변경시키기 위한 방법 및 조성물
CN121108345A (zh) 抗cd45ro单克隆抗体及其在制备治疗免疫性疾病药物中的应用
CN118076635A (zh) 抗il-27抗体和其用途
TW202140012A (zh) 用於治療尿路上皮癌的fgfr酪胺酸激酶抑制劑和抗pd1藥劑
BR112022011749B1 (pt) Anticorpo ou porção de ligação ao antígeno do mesmo que se liga especificamente a um ou mais aminoácidos dentro do domínio n-terminal extracelular do ccr8 humano, anticorpo biespecífico ou multiespecífico, engajador de células t bispecífico (bite), anticorpo biparatópico, imunoconjugado e composição farmacêutica
HK40003978A (en) Antibodies, pharmaceutical compositions and methods

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20211022

CF01 Termination of patent right due to non-payment of annual fee