CA2983937A1 - Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens - Google Patents
Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigensInfo
- Publication number
- CA2983937A1 CA2983937A1 CA2983937A CA2983937A CA2983937A1 CA 2983937 A1 CA2983937 A1 CA 2983937A1 CA 2983937 A CA2983937 A CA 2983937A CA 2983937 A CA2983937 A CA 2983937A CA 2983937 A1 CA2983937 A1 CA 2983937A1
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- til
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| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| WO2017214557A1 (en) * | 2016-06-10 | 2017-12-14 | Counsyl, Inc. | Nucleic acid sequencing adapters and uses thereof |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| MY201744A (en) | 2016-12-23 | 2024-03-15 | Visterra Inc | Binding polypeptides and methods of making the same |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4029939B1 (en) | 2017-01-30 | 2023-06-28 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| CN111148849A (zh) | 2017-05-26 | 2020-05-12 | 阿布维托有限责任公司 | 高通量多核苷酸文库测序和转录组分析 |
| KR20250096881A (ko) | 2017-08-09 | 2025-06-27 | 주노 쎄러퓨티크스 인코퍼레이티드 | 유전자 조작된 세포를 제조하기 위한 방법 및 조성물 |
| US20200292526A1 (en) | 2017-09-07 | 2020-09-17 | Juno Therapeutics, Inc. | Methods of identifying cellular attributes related to outcomes associated with cell therapy |
| WO2019084538A1 (en) * | 2017-10-27 | 2019-05-02 | Board Of Regents, The University Of Texas System | TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF |
| WO2019118937A1 (en) | 2017-12-15 | 2019-06-20 | Juno Therapeutics, Inc. | Anti-cct5 binding molecules and methods of use thereof |
| SG11202007686VA (en) | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| WO2019241249A1 (en) * | 2018-06-11 | 2019-12-19 | The Brigham And Women's Hospital, Inc. | Single cell cloning approaches for biological studies |
| JP7145980B2 (ja) | 2018-06-15 | 2022-10-03 | エフ．ホフマン－ラ ロシュ アーゲー | 腫瘍浸潤リンパ球にて発現するｔ細胞受容体により認識される抗原を識別するためのシステム |
| CN116144706A (zh) | 2018-08-13 | 2023-05-23 | 根路径基因组学公司 | 成对的二分免疫受体多核苷酸的高通量克隆及其应用 |
| EP3867406B1 (en) * | 2018-10-19 | 2023-01-04 | Akoya Biosciences, Inc. | Detection of co-occurring receptor-coding nucleic acid segments |
| MX2021009087A (es) | 2019-01-29 | 2021-09-08 | Juno Therapeutics Inc | Anticuerpos y receptores quimericos de antigenos especificos para receptor 1 huerfano tipo receptor tirosina-cinasa (ror1). |
| AU2020253491A1 (en) | 2019-04-05 | 2021-10-28 | Rootpath Genomics, Inc. | Compositions and methods for T-cell receptor gene assembly |
| CA3157605A1 (en) * | 2019-11-13 | 2021-05-20 | Volker Schellenberger | Barcoded xten polypeptides and compositions thereof, and methods for making and using the same |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| CN111218502B (zh) * | 2020-04-23 | 2020-07-21 | 圣湘生物科技股份有限公司 | 提升qPCR检测性能的组合物、反应液、用途及方法 |
| JP7727662B2 (ja) | 2020-05-13 | 2025-08-21 | ジュノー セラピューティクス インコーポレイテッド | 臨床応答に関連する特徴量の特定方法およびその使用 |
| WO2022081643A2 (en) * | 2020-10-13 | 2022-04-21 | 10X Genomics, Inc. | Compositions and methods for generating recombinant antigen binding molecules from single cells |
| CN114464253B (zh) * | 2022-03-03 | 2023-03-10 | 予果生物科技（北京）有限公司 | 基于长读长测序进行实时病原检测的方法、系统和应用 |
| US20250295696A1 (en) * | 2022-05-03 | 2025-09-25 | 3T Biosciences, Inc. | T-cell target discovery |
| WO2024015862A1 (en) * | 2022-07-13 | 2024-01-18 | 10X Genomics, Inc. | Methods for characterization of antigen-binding molecules from biological samples |
| WO2024192051A1 (en) | 2023-03-13 | 2024-09-19 | Turnstone Biologics Corp. | Composition of selected tumor infiltrating lymphocytes and related methods of producing and using the same |
| GB2629157A (en) * | 2023-04-18 | 2024-10-23 | The Institute Of Cancer Res Royal Cancer Hospital | T cell receptor sequencing |
| WO2025080865A1 (en) | 2023-10-11 | 2025-04-17 | Turnstone Biologics Corp. | Combination of tumor infiltrating lymphocytes (til) and low dose radiation |
| WO2025096638A2 (en) | 2023-10-30 | 2025-05-08 | Turnstone Biologics Corp. | Genetically modified tumor infilitrating lymphocytes and methods of producing and using the same |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990004648A1 (en) | 1988-10-20 | 1990-05-03 | Alexander Alan Morley | Method for diagnosis of monoclonality in leukaemia and lymphoma |
| US20070160994A1 (en) * | 2003-12-15 | 2007-07-12 | Institut Pasteur | Repertoire determination of a lymphocyte b population |
| US8268564B2 (en) * | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| GB2483810B (en) | 2008-11-07 | 2012-09-05 | Sequenta Inc | Methods for correlating clonotypes with diseases in a population |
| WO2011037990A1 (en) * | 2009-09-22 | 2011-03-31 | President And Fellows Of Harvard College | Entangled mate sequencing |
| EP2670863B1 (en) * | 2011-01-31 | 2018-06-27 | H. Hoffnabb-La Roche Ag | Methods of identifying multiple epitopes in cells |
| EP3421591B1 (en) * | 2011-04-28 | 2023-09-27 | The Board of Trustees of the Leland Stanford Junior University | Identification of polynucleotides associated with a sample |
| SI2755997T1 (sl) * | 2011-09-15 | 2018-11-30 | The United States of America, as represented by Secretary, Department of Health and Human Services, Office of Technology Transfer, National Institutes of Health | T-celični receptorji, ki prepoznajo HLA-A1- ali HLA-CW7-omejeni mage |
| EP2768982A4 (en) | 2011-10-21 | 2015-06-03 | Adaptive Biotechnologies Corp | QUANTIFICATION OF ADAPTIVE IMMUNOCELL GENOMES IN A COMPLEX MIX OF CELLS |
| US9708654B2 (en) * | 2012-06-15 | 2017-07-18 | Board Of Regents, The University Of Texas System | High throughput sequencing of multiple transcripts |
| WO2014130685A1 (en) * | 2013-02-22 | 2014-08-28 | Sequenta, Inc. | Rare clonotypes and uses thereof |
| US10119134B2 (en) * | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| SG11201506991VA (en) * | 2013-03-15 | 2015-10-29 | Adaptive Biotechnologies Corp | Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set |
| CN110964796B (zh) | 2013-08-28 | 2024-04-05 | 贝克顿迪金森公司 | 大规模平行单细胞分析 |
| EP3626748A1 (en) * | 2014-01-13 | 2020-03-25 | Stephen J. Forman | Chimeric antigen receptors (cars) having mutations in the fc spacer region and methods for their use |
| SG10201911069WA (en) | 2014-09-15 | 2020-01-30 | Abvitro Llc | High-throughput nucleotide library sequencing |
| US11111519B2 (en) * | 2015-02-04 | 2021-09-07 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
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| AU2016253964A1 (en) | 2017-11-09 |
| CN107849560A (zh) | 2018-03-27 |
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