JP2018088924A5 - - Google Patents
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- JP2018088924A5 JP2018088924A5 JP2017253460A JP2017253460A JP2018088924A5 JP 2018088924 A5 JP2018088924 A5 JP 2018088924A5 JP 2017253460 A JP2017253460 A JP 2017253460A JP 2017253460 A JP2017253460 A JP 2017253460A JP 2018088924 A5 JP2018088924 A5 JP 2018088924A5
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- JP
- Japan
- Prior art keywords
- tso
- locked
- kit
- residue
- nucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 125000001921 locked nucleotide group Chemical group 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 3
- 108091034117 Oligonucleotide Proteins 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 3
- 102000053602 DNA Human genes 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical group C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 2
- 159000000003 magnesium salts Chemical group 0.000 claims 2
- 229910052751 metal Inorganic materials 0.000 claims 2
- 239000002184 metal Substances 0.000 claims 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims 2
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 claims 1
- 229930024421 Adenine Natural products 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 102100034343 Integrase Human genes 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 229960000643 adenine Drugs 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 229960003237 betaine Drugs 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000010804 cDNA synthesis Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 229940104302 cytosine Drugs 0.000 claims 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
- 229940113082 thymine Drugs 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361869220P | 2013-08-23 | 2013-08-23 | |
| US61/869,220 | 2013-08-23 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016536474A Division JP6336080B2 (ja) | 2013-08-23 | 2014-08-22 | 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018088924A JP2018088924A (ja) | 2018-06-14 |
| JP2018088924A5 true JP2018088924A5 (enExample) | 2018-07-26 |
| JP6603699B2 JP6603699B2 (ja) | 2019-11-06 |
Family
ID=52484185
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016536474A Active JP6336080B2 (ja) | 2013-08-23 | 2014-08-22 | 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 |
| JP2017253460A Active JP6603699B2 (ja) | 2013-08-23 | 2017-12-28 | 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016536474A Active JP6336080B2 (ja) | 2013-08-23 | 2014-08-22 | 鋳型切り替え反応を用いるｃＤＮＡ合成および単一細胞トランスクリプトームプロファイリングのための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10266894B2 (enExample) |
| EP (1) | EP3036336B1 (enExample) |
| JP (2) | JP6336080B2 (enExample) |
| CN (2) | CN105579587A (enExample) |
| CA (1) | CA2921603C (enExample) |
| DK (1) | DK3036336T3 (enExample) |
| ES (1) | ES2827307T3 (enExample) |
| WO (1) | WO2015027135A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10266894B2 (en) * | 2013-08-23 | 2019-04-23 | Ludwig Institute For Cancer Research Ltd | Methods and compositions for cDNA synthesis and single-cell transcriptome profiling using template switching reaction |
| ES2912183T3 (es) * | 2013-12-30 | 2022-05-24 | Atreca Inc | Análisis de ácidos nucleicos asociados a células individuales utilizando códigos de barras de ácidos nucleicos |
| US10870848B2 (en) | 2015-09-15 | 2020-12-22 | Takara Bio Usa, Inc. | Methods for preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample and compositions for practicing the same |
| KR20180097536A (ko) | 2015-11-04 | 2018-08-31 | 아트레카, 인크. | 단일 세포와 연관된 핵산의 분석을 위한 핵산 바코드의 조합 세트 |
| WO2017214417A1 (en) | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| CN106498040B (zh) * | 2016-10-12 | 2018-10-23 | 浙江大学 | 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法 |
| EP3985122A1 (en) * | 2016-11-10 | 2022-04-20 | Takara Bio USA, Inc. | Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein |
| KR102489902B1 (ko) | 2016-11-11 | 2023-01-19 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 샘플을 프로세싱하는 방법 |
| CN106868005B (zh) * | 2017-03-17 | 2020-07-21 | 中山大学附属肿瘤医院 | 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法 |
| AU2018328324B2 (en) | 2017-09-11 | 2024-12-12 | Ludwig Institute For Cancer Research Ltd | Selective labeling of 5-methylcytosine in circulating cell-free DNA |
| CN108103055B (zh) * | 2018-01-09 | 2021-05-28 | 序康医疗科技（苏州）有限公司 | 一种单细胞rna逆转录与文库构建的方法 |
| WO2019152860A1 (en) | 2018-02-02 | 2019-08-08 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Random amplification methods for extremely low input nucleic acids |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| US20220033811A1 (en) | 2018-12-28 | 2022-02-03 | Biobloxx Ab | Method and kit for preparing complementary dna |
| CN113811610A (zh) * | 2019-05-09 | 2021-12-17 | 加利福尼亚太平洋生物科学股份有限公司 | 用于改进cDNA合成的组合物和方法 |
| WO2021006353A1 (ja) | 2019-07-11 | 2021-01-14 | 学校法人東京理科大学 | 固相担体を用いた核酸増幅方法 |
| US20230193254A1 (en) * | 2020-05-20 | 2023-06-22 | Chan Zuckerberg Biohub, Inc. | Total rna profiling of biological samples and single cells |
| WO2023241721A1 (zh) * | 2022-06-17 | 2023-12-21 | 复旦大学 | 基于拓扑捕获的rna样本处理系统 |
| KR20250094720A (ko) | 2023-03-09 | 2025-06-25 | 이뮤노제네텍스 가부시키가이샤 | 상보 dna쇄를 증폭하는 방법 |
| CN116497105B (zh) * | 2023-06-28 | 2023-09-29 | 浙江大学 | 基于末端转移酶的单细胞转录组测序试剂盒及测序方法 |
| CN119776495A (zh) * | 2024-12-31 | 2025-04-08 | 北京普译生物科技有限公司 | 一种cDNA建库中的模板转换方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| ATE314488T1 (de) | 1999-03-18 | 2006-01-15 | Exiqon As | Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben |
| EP3998346A1 (en) * | 2009-03-30 | 2022-05-18 | Illumina, Inc. | Gene expression analysis in single cells |
| CA2821559C (en) | 2011-01-28 | 2017-01-31 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| WO2012106546A2 (en) * | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| US9260753B2 (en) * | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| US10266894B2 (en) * | 2013-08-23 | 2019-04-23 | Ludwig Institute For Cancer Research Ltd | Methods and compositions for cDNA synthesis and single-cell transcriptome profiling using template switching reaction |
-
2014
- 2014-08-22 US US14/912,556 patent/US10266894B2/en active Active
- 2014-08-22 ES ES14837396T patent/ES2827307T3/es active Active
- 2014-08-22 CA CA2921603A patent/CA2921603C/en active Active
- 2014-08-22 CN CN201480053360.6A patent/CN105579587A/zh active Pending
- 2014-08-22 JP JP2016536474A patent/JP6336080B2/ja active Active
- 2014-08-22 DK DK14837396.2T patent/DK3036336T3/da active
- 2014-08-22 WO PCT/US2014/052233 patent/WO2015027135A1/en not_active Ceased
- 2014-08-22 EP EP14837396.2A patent/EP3036336B1/en active Active
- 2014-08-22 CN CN202211158053.7A patent/CN115558661A/zh active Pending
-
2017
- 2017-12-28 JP JP2017253460A patent/JP6603699B2/ja active Active
-
2019
- 2019-02-19 US US16/279,245 patent/US20190264284A1/en not_active Abandoned
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