JP2017536103A5 - - Google Patents
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- Publication number
- JP2017536103A5 JP2017536103A5 JP2017520357A JP2017520357A JP2017536103A5 JP 2017536103 A5 JP2017536103 A5 JP 2017536103A5 JP 2017520357 A JP2017520357 A JP 2017520357A JP 2017520357 A JP2017520357 A JP 2017520357A JP 2017536103 A5 JP2017536103 A5 JP 2017536103A5
- Authority
- JP
- Japan
- Prior art keywords
- analyte
- polynucleotide
- pore
- membrane
- transmembrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011148 porous material Substances 0.000 claims 23
- 239000012491 analyte Substances 0.000 claims 22
- 238000000034 method Methods 0.000 claims 22
- 239000002157 polynucleotide Substances 0.000 claims 18
- 102000040430 polynucleotide Human genes 0.000 claims 18
- 108091033319 polynucleotide Proteins 0.000 claims 18
- 239000012528 membrane Substances 0.000 claims 11
- 239000011859 microparticle Substances 0.000 claims 11
- 238000005259 measurement Methods 0.000 claims 6
- 239000010419 fine particle Substances 0.000 claims 5
- 230000003993 interaction Effects 0.000 claims 3
- 101710092462 Alpha-hemolysin Proteins 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 230000002209 hydrophobic effect Effects 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- 108091005703 transmembrane proteins Proteins 0.000 claims 2
- 102000035160 transmembrane proteins Human genes 0.000 claims 2
- 101710174798 Lysenin Proteins 0.000 claims 1
- 241000187480 Mycobacterium smegmatis Species 0.000 claims 1
- 108010013381 Porins Proteins 0.000 claims 1
- 239000000919 ceramic Substances 0.000 claims 1
- 238000012512 characterization method Methods 0.000 claims 1
- 238000009792 diffusion process Methods 0.000 claims 1
- 230000005684 electric field Effects 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 230000005484 gravity Effects 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000007739 porin activity proteins Human genes 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1418469.1A GB201418469D0 (en) | 2014-10-17 | 2014-10-17 | Method |
| GB1418469.1 | 2014-10-17 | ||
| PCT/GB2015/052919 WO2016059375A1 (en) | 2014-10-17 | 2015-10-06 | Methods for delivering an analyte to transmembrane pores |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017536103A JP2017536103A (ja) | 2017-12-07 |
| JP2017536103A5 true JP2017536103A5 (cg-RX-API-DMAC7.html) | 2018-11-22 |
| JP6721581B2 JP6721581B2 (ja) | 2020-07-15 |
Family
ID=52013164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017520357A Active JP6721581B2 (ja) | 2014-10-17 | 2015-10-06 | 分析物を膜貫通ポアに送達する方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US10760114B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3207157B2 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6721581B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102429381B1 (cg-RX-API-DMAC7.html) |
| CN (2) | CN114107457B (cg-RX-API-DMAC7.html) |
| GB (1) | GB201418469D0 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2016059375A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP4273092A3 (en) | 2011-05-27 | 2024-01-17 | Oxford Nanopore Technologies plc | Method and apparatus for determining the presence, absence or characteristics of an analyte |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201418469D0 (en) * | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| CN107429216B (zh) | 2015-02-10 | 2023-01-24 | 美国亚德诺半导体公司 | 用于检测分子和结合能的装置和方法 |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| JP7108548B2 (ja) * | 2016-05-31 | 2022-07-28 | エフ．ホフマン－ラ ロシュ アーゲー | 核酸分子を分析するための方法およびデバイス |
| US10444179B2 (en) | 2016-08-10 | 2019-10-15 | Multerra Bio, Inc. | Apparatuses and methods for detecting molecules and binding energy |
| US9816988B1 (en) * | 2016-08-10 | 2017-11-14 | Multerra Bio, Inc. | Apparatuses and methods for detecting molecules and binding energy |
| GB201616590D0 (en) * | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707140D0 (en) * | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB2569977A (en) * | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| GB201801768D0 (en) * | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| CN112119033A (zh) * | 2018-02-09 | 2020-12-22 | 俄亥俄州国家创新基金会 | 源自噬菌体的纳米孔传感器 |
| GB201808554D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| WO2020084705A1 (ja) * | 2018-10-24 | 2020-04-30 | 株式会社日立ハイテク | 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法 |
| CN113166703A (zh) * | 2018-11-21 | 2021-07-23 | 深圳华大生命科学研究院 | 核酸分子检测方法、检测装置和检测系统 |
| WO2020150140A1 (en) * | 2019-01-16 | 2020-07-23 | Armonica Technologies, Inc. | Manipulating the translation of dna strands across and through nanopore sequencing systems using raman signatures to identify dna bases and methods |
| GB201907244D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| WO2020241752A1 (en) | 2019-05-28 | 2020-12-03 | The University Of Tokyo | Analyzing apparatus and method using a pore device |
| GB201913997D0 (en) * | 2019-09-27 | 2019-11-13 | Oxford Nanopore Tech Ltd | Method |
| WO2021113412A1 (en) * | 2019-12-02 | 2021-06-10 | The Regents Of The University Of California | Lipid-DNA Labeling of Lipid Bilayer Particles for Amplification Quantitation |
| GB201917742D0 (en) * | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| WO2022165276A1 (en) | 2021-01-29 | 2022-08-04 | Armonica Technologies, Inc. | Enhancement structures for surface-enhanced raman scattering |
| CN113999291B (zh) * | 2021-12-28 | 2022-04-15 | 北京齐碳科技有限公司 | 嵌入接头、锚定分子、分子膜、装置、方法及应用 |
| GB202307494D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Ltd | Nanopore sequencing and preparation modular instrument |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US5712126A (en) | 1995-08-01 | 1998-01-27 | Yale University | Analysis of gene expression by display of 3-end restriction fragments of CDNA |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US7527787B2 (en) | 2005-10-19 | 2009-05-05 | Ibc Pharmaceuticals, Inc. | Multivalent immunoglobulin-based bioactive assemblies |
| EP1192103A1 (en) | 1999-06-22 | 2002-04-03 | President And Fellows of Harvard College | Control of solid state dimensional features |
| US6849404B2 (en) | 2001-05-07 | 2005-02-01 | Bioneer Corporation | Polymerase chain reaction of DNA of which base sequence is completely unidentified |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
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| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| AU2008217578A1 (en) | 2007-02-20 | 2008-08-28 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor system |
| EP3798317B1 (en) | 2007-04-04 | 2024-01-03 | The Regents of the University of California | Compositions, devices, systems, and methods for using a nanopore |
| US9395352B2 (en) † | 2007-04-06 | 2016-07-19 | Arizona Board Of Regents On Behalf Of Arizona State University | Devices and methods for target molecule characterization |
| EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| EP2014761B1 (en) † | 2007-06-22 | 2016-09-28 | Sony Deutschland GmbH | A device for processing an analyte and a method of processing and/or detecting an analyte using said device |
| WO2009035647A1 (en) † | 2007-09-12 | 2009-03-19 | President And Fellows Of Harvard College | High-resolution molecular graphene sensor comprising an aperture in the graphene layer |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8628940B2 (en) | 2008-09-24 | 2014-01-14 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| WO2009151788A2 (en) | 2008-04-22 | 2009-12-17 | The Washington University | Universal anchor peptide for nanoparticles |
| JP2012500620A (ja) | 2008-04-24 | 2012-01-12 | ザ トラスティーズ オブ コロンビア ユニバーシティー イン ザ シティー オブ ニューヨーク | Ｄｎａ分子組織化のための幾何学的パターンおよび脂質二重層、ならびにその使用 |
| US20110229877A1 (en) | 2008-07-07 | 2011-09-22 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| WO2010004273A1 (en) | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| EP2313758B1 (en) | 2008-07-17 | 2012-02-01 | Koninklijke Philips Electronics N.V. | Nanopore device and a method for nucleic acid analysis |
| CN102216783B (zh) * | 2008-09-22 | 2015-04-01 | 华盛顿大学 | Msp纳米微孔和相关方法 |
| GB0820927D0 (en) * | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| CN102365367A (zh) † | 2009-01-29 | 2012-02-29 | 斯特拉托斯基因公司 | 通过展开进行高通量核酸测序和相关方法 |
| CN102482330A (zh) | 2009-01-30 | 2012-05-30 | 牛津纳米孔技术有限公司 | 酶突变体 |
| KR101797773B1 (ko) | 2009-01-30 | 2017-11-15 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 막횡단 시퀀싱에서 핵산 구축물용 어댑터 |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| CA2758617C (en) | 2009-04-20 | 2017-10-03 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor array |
| BR112012013074B1 (pt) | 2009-12-01 | 2018-09-18 | Oxford Nanopore Technologies Limited | instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica |
| EP2539707B1 (en) * | 2010-02-23 | 2021-06-30 | University Of Washington | Artificial mycolic acid membranes |
| JP5764296B2 (ja) † | 2010-03-31 | 2015-08-19 | 株式会社日立ハイテクノロジーズ | 生体ポリマーの特性解析法 |
| US20140051068A1 (en) | 2010-09-07 | 2014-02-20 | The Regents Of The University Of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| EP3000883B8 (en) | 2010-11-12 | 2018-02-28 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
| AU2012215135B9 (en) | 2011-02-11 | 2017-03-09 | Oxford Nanopore Technologies Limited | Mutant pores |
| US9347929B2 (en) | 2011-03-01 | 2016-05-24 | The Regents Of The University Of Michigan | Controlling translocation through nanopores with fluid wall |
| EP2699903B1 (en) † | 2011-04-20 | 2018-07-18 | Life Technologies Corporation | Methods, compositions and systems for sample deposition |
| EP4273092A3 (en) * | 2011-05-27 | 2024-01-17 | Oxford Nanopore Technologies plc | Method and apparatus for determining the presence, absence or characteristics of an analyte |
| CA2843136C (en) * | 2011-07-25 | 2020-12-29 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| EP2573554A1 (en) † | 2011-09-21 | 2013-03-27 | Nxp B.V. | Apparatus and method for bead detection |
| CA2849624C (en) | 2011-09-23 | 2021-05-25 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| EP2768977B1 (en) | 2011-10-21 | 2015-09-23 | Oxford Nanopore Technologies Limited | Method of characterizing a target polynucleotide using a pore and a hel308 helicase |
| US9267917B2 (en) † | 2011-11-04 | 2016-02-23 | Pacific Biosciences Of California, Inc. | Nanopores in zero mode waveguides |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| WO2013098562A2 (en) | 2011-12-29 | 2013-07-04 | Oxford Nanopore Technologies Limited | Enzyme method |
| WO2013119784A1 (en) * | 2012-02-08 | 2013-08-15 | Brown University | Methods of sequencing nucleic acids using nanopores and active kinetic proofreading |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| EP2875128B8 (en) | 2012-07-19 | 2020-06-24 | Oxford Nanopore Technologies Limited | Modified helicases |
| JP6429773B2 (ja) | 2012-07-19 | 2018-11-28 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素構築物 |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| EP2895618B1 (en) * | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| EP2911771B1 (en) | 2012-10-26 | 2017-01-18 | Oxford Nanopore Technologies Limited | Droplet interfaces |
| CA2890515C (en) * | 2012-11-09 | 2021-11-09 | Stratos Genomics, Inc. | Concentrating a target molecule for sensing by a nanopore |
| WO2014130686A2 (en) | 2013-02-20 | 2014-08-28 | Eve Biomedical, Inc. | Methods and compositions for nanostructure-based nucleic acid sequencing |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| SG11201507138RA (en) | 2013-03-08 | 2015-10-29 | Oxford Nanopore Tech Ltd | Enzyme stalling method |
| JP6677640B2 (ja) | 2013-10-18 | 2020-04-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 修飾酵素 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN109797199A (zh) * | 2013-10-23 | 2019-05-24 | 吉尼亚科技公司 | 使用纳米孔的高速分子感测 |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201418469D0 (en) * | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
-
2014
- 2014-10-17 GB GBGB1418469.1A patent/GB201418469D0/en not_active Ceased
-
2015
- 2015-10-06 CN CN202111480105.8A patent/CN114107457B/zh active Active
- 2015-10-06 CN CN201580068371.6A patent/CN107002151B/zh active Active
- 2015-10-06 KR KR1020177013135A patent/KR102429381B1/ko active Active
- 2015-10-06 JP JP2017520357A patent/JP6721581B2/ja active Active
- 2015-10-06 EP EP15790630.6A patent/EP3207157B2/en active Active
- 2015-10-06 WO PCT/GB2015/052919 patent/WO2016059375A1/en not_active Ceased
- 2015-10-06 US US15/519,606 patent/US10760114B2/en active Active
-
2020
- 2020-06-16 US US16/902,306 patent/US11613771B2/en active Active
-
2023
- 2023-02-17 US US18/170,596 patent/US20240124915A9/en active Pending
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