KR102429381B1 - 분석물을 막횡단 세공에 전달하는 방법 - Google Patents

분석물을 막횡단 세공에 전달하는 방법 Download PDF

Info

Publication number
KR102429381B1
KR102429381B1 KR1020177013135A KR20177013135A KR102429381B1 KR 102429381 B1 KR102429381 B1 KR 102429381B1 KR 1020177013135 A KR1020177013135 A KR 1020177013135A KR 20177013135 A KR20177013135 A KR 20177013135A KR 102429381 B1 KR102429381 B1 KR 102429381B1
Authority
KR
South Korea
Prior art keywords
leu
ala
polynucleotide
gly
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020177013135A
Other languages
English (en)
Korean (ko)
Other versions
KR20170072914A (ko
Inventor
클리브 브라운
다니엘 라이언 제럴드
앤디 헤론
다니엘 존 터너
제임스 화이트
Original Assignee
옥스포드 나노포어 테크놀로지즈 피엘씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52013164&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR102429381(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 옥스포드 나노포어 테크놀로지즈 피엘씨 filed Critical 옥스포드 나노포어 테크놀로지즈 피엘씨
Publication of KR20170072914A publication Critical patent/KR20170072914A/ko
Application granted granted Critical
Publication of KR102429381B1 publication Critical patent/KR102429381B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728Investigating individual cells, e.g. by patch clamp, voltage clamp
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00Details of magnetic or electrostatic separation
    • B03C2201/18Magnetic separation whereby the particles are suspended in a liquid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00Reactions characterised by treatment of reaction samples
    • C12Q2523/30Characterised by physical treatment
    • C12Q2523/303Applying a physical force on a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/197Modifications characterised by incorporating a spacer/coupling moiety
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/143Magnetism, e.g. magnetic label
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155Particles of a defined size, e.g. nanoparticles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/157Nanotubes or nanorods
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60Detection means characterised by use of a special device
    • C12Q2565/631Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
KR1020177013135A 2014-10-17 2015-10-06 분석물을 막횡단 세공에 전달하는 방법 Active KR102429381B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1418469.1A GB201418469D0 (en) 2014-10-17 2014-10-17 Method
GB1418469.1 2014-10-17
PCT/GB2015/052919 WO2016059375A1 (en) 2014-10-17 2015-10-06 Methods for delivering an analyte to transmembrane pores

Publications (2)

Publication Number Publication Date
KR20170072914A KR20170072914A (ko) 2017-06-27
KR102429381B1 true KR102429381B1 (ko) 2022-08-03

Family

ID=52013164

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020177013135A Active KR102429381B1 (ko) 2014-10-17 2015-10-06 분석물을 막횡단 세공에 전달하는 방법

Country Status (7)

Country Link
US (3) US10760114B2 (cg-RX-API-DMAC7.html)
EP (1) EP3207157B2 (cg-RX-API-DMAC7.html)
JP (1) JP6721581B2 (cg-RX-API-DMAC7.html)
KR (1) KR102429381B1 (cg-RX-API-DMAC7.html)
CN (2) CN114107457B (cg-RX-API-DMAC7.html)
GB (1) GB201418469D0 (cg-RX-API-DMAC7.html)
WO (1) WO2016059375A1 (cg-RX-API-DMAC7.html)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4273092A3 (en) 2011-05-27 2024-01-17 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201418469D0 (en) * 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
CN107429216B (zh) 2015-02-10 2023-01-24 美国亚德诺半导体公司 用于检测分子和结合能的装置和方法
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
JP7108548B2 (ja) * 2016-05-31 2022-07-28 エフ.ホフマン-ラ ロシュ アーゲー 核酸分子を分析するための方法およびデバイス
US10444179B2 (en) 2016-08-10 2019-10-15 Multerra Bio, Inc. Apparatuses and methods for detecting molecules and binding energy
US9816988B1 (en) * 2016-08-10 2017-11-14 Multerra Bio, Inc. Apparatuses and methods for detecting molecules and binding energy
GB201616590D0 (en) * 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB201707140D0 (en) * 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) * 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
CN112119033A (zh) * 2018-02-09 2020-12-22 俄亥俄州国家创新基金会 源自噬菌体的纳米孔传感器
GB201808554D0 (en) 2018-05-24 2018-07-11 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
WO2020084705A1 (ja) * 2018-10-24 2020-04-30 株式会社日立ハイテク 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法
CN113166703A (zh) * 2018-11-21 2021-07-23 深圳华大生命科学研究院 核酸分子检测方法、检测装置和检测系统
WO2020150140A1 (en) * 2019-01-16 2020-07-23 Armonica Technologies, Inc. Manipulating the translation of dna strands across and through nanopore sequencing systems using raman signatures to identify dna bases and methods
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
WO2020241752A1 (en) 2019-05-28 2020-12-03 The University Of Tokyo Analyzing apparatus and method using a pore device
GB201913997D0 (en) * 2019-09-27 2019-11-13 Oxford Nanopore Tech Ltd Method
WO2021113412A1 (en) * 2019-12-02 2021-06-10 The Regents Of The University Of California Lipid-DNA Labeling of Lipid Bilayer Particles for Amplification Quantitation
GB201917742D0 (en) * 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
WO2022165276A1 (en) 2021-01-29 2022-08-04 Armonica Technologies, Inc. Enhancement structures for surface-enhanced raman scattering
CN113999291B (zh) * 2021-12-28 2022-04-15 北京齐碳科技有限公司 嵌入接头、锚定分子、分子膜、装置、方法及应用
GB202307494D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Ltd Nanopore sequencing and preparation modular instrument

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012516146A (ja) * 2009-01-30 2012-07-19 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US20140134618A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US7527787B2 (en) 2005-10-19 2009-05-05 Ibc Pharmaceuticals, Inc. Multivalent immunoglobulin-based bioactive assemblies
EP1192103A1 (en) 1999-06-22 2002-04-03 President And Fellows of Harvard College Control of solid state dimensional features
US6849404B2 (en) 2001-05-07 2005-02-01 Bioneer Corporation Polymerase chain reaction of DNA of which base sequence is completely unidentified
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
CA2603352C (en) 2005-04-06 2013-10-01 Jene Golovchenko Molecular characterization with carbon nanotube control
KR100691806B1 (ko) * 2005-08-04 2007-03-12 삼성전자주식회사 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
AU2008217578A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
EP3798317B1 (en) 2007-04-04 2024-01-03 The Regents of the University of California Compositions, devices, systems, and methods for using a nanopore
US9395352B2 (en) 2007-04-06 2016-07-19 Arizona Board Of Regents On Behalf Of Arizona State University Devices and methods for target molecule characterization
EP3543357A1 (en) 2007-05-08 2019-09-25 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
EP2014761B1 (en) 2007-06-22 2016-09-28 Sony Deutschland GmbH A device for processing an analyte and a method of processing and/or detecting an analyte using said device
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009151788A2 (en) 2008-04-22 2009-12-17 The Washington University Universal anchor peptide for nanoparticles
JP2012500620A (ja) 2008-04-24 2012-01-12 ザ トラスティーズ オブ コロンビア ユニバーシティー イン ザ シティー オブ ニューヨーク Dna分子組織化のための幾何学的パターンおよび脂質二重層、ならびにその使用
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
EP2313758B1 (en) 2008-07-17 2012-02-01 Koninklijke Philips Electronics N.V. Nanopore device and a method for nucleic acid analysis
CN102216783B (zh) * 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
CN102365367A (zh) 2009-01-29 2012-02-29 斯特拉托斯基因公司 通过展开进行高通量核酸测序和相关方法
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CA2758617C (en) 2009-04-20 2017-10-03 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
EP2539707B1 (en) * 2010-02-23 2021-06-30 University Of Washington Artificial mycolic acid membranes
JP5764296B2 (ja) 2010-03-31 2015-08-19 株式会社日立ハイテクノロジーズ 生体ポリマーの特性解析法
US20140051068A1 (en) 2010-09-07 2014-02-20 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
EP3000883B8 (en) 2010-11-12 2018-02-28 Gen9, Inc. Methods and devices for nucleic acids synthesis
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
US9347929B2 (en) 2011-03-01 2016-05-24 The Regents Of The University Of Michigan Controlling translocation through nanopores with fluid wall
EP2699903B1 (en) 2011-04-20 2018-07-18 Life Technologies Corporation Methods, compositions and systems for sample deposition
EP4273092A3 (en) * 2011-05-27 2024-01-17 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
CA2843136C (en) * 2011-07-25 2020-12-29 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP2573554A1 (en) 2011-09-21 2013-03-27 Nxp B.V. Apparatus and method for bead detection
CA2849624C (en) 2011-09-23 2021-05-25 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2768977B1 (en) 2011-10-21 2015-09-23 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a pore and a hel308 helicase
US9267917B2 (en) 2011-11-04 2016-02-23 Pacific Biosciences Of California, Inc. Nanopores in zero mode waveguides
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2013119784A1 (en) * 2012-02-08 2013-08-15 Brown University Methods of sequencing nucleic acids using nanopores and active kinetic proofreading
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP2895618B1 (en) * 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2911771B1 (en) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
WO2014130686A2 (en) 2013-02-20 2014-08-28 Eve Biomedical, Inc. Methods and compositions for nanostructure-based nucleic acid sequencing
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
JP6677640B2 (ja) 2013-10-18 2020-04-08 オックスフォード ナノポール テクノロジーズ リミテッド 修飾酵素
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN109797199A (zh) * 2013-10-23 2019-05-24 吉尼亚科技公司 使用纳米孔的高速分子感测
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201418469D0 (en) * 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012516146A (ja) * 2009-01-30 2012-07-19 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
US20140134618A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore

Also Published As

Publication number Publication date
US10760114B2 (en) 2020-09-01
US11613771B2 (en) 2023-03-28
JP6721581B2 (ja) 2020-07-15
JP2017536103A (ja) 2017-12-07
US20210147904A1 (en) 2021-05-20
CN107002151A (zh) 2017-08-01
EP3207157B1 (en) 2019-12-04
CN114107457A (zh) 2022-03-01
US20240124915A9 (en) 2024-04-18
WO2016059375A1 (en) 2016-04-21
CN107002151B (zh) 2022-04-01
US20170253910A1 (en) 2017-09-07
EP3207157B2 (en) 2023-04-05
GB201418469D0 (en) 2014-12-03
KR20170072914A (ko) 2017-06-27
CN114107457B (zh) 2025-07-08
EP3207157A1 (en) 2017-08-23
US20230357821A1 (en) 2023-11-09

Similar Documents

Publication Publication Date Title
US20230357821A1 (en) Methods for delivering an analyte to transmembrane pores
US11649490B2 (en) Method of target molecule characterisation using a molecular pore
EP3464624B1 (en) Method of nanopore sequencing of concatenated nucleic acids
EP3548636B1 (en) Methods and systems for characterizing analytes using nanopores
US20250207188A1 (en) Method
EP3097204B1 (en) Method for controlling the movement of a polynucleotide through a transmembrane pore
EP3802877B1 (en) Method
CA3062111A1 (en) Transmembrane pore consisting of two csgg pores
KR20140048142A (ko) 커플링 방법
JP2017503517A (ja) 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000

D14-X000 Search report completed

St.27 status event code: A-1-2-D10-D14-srh-X000

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 4