CN113166703A - 核酸分子检测方法、检测装置和检测系统 - Google Patents
核酸分子检测方法、检测装置和检测系统 Download PDFInfo
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- CN113166703A CN113166703A CN201880099314.8A CN201880099314A CN113166703A CN 113166703 A CN113166703 A CN 113166703A CN 201880099314 A CN201880099314 A CN 201880099314A CN 113166703 A CN113166703 A CN 113166703A
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- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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Abstract
一种核酸分子检测方法、检测装置和检测系统，该方法包括：向含有待检测核酸分子的溶液中施加电压，使待检测核酸分子在电压作用下通过置于溶液中的纳米孔；检测待检测核酸分子通过纳米孔时的电流变化，根据电流变化确定待检测核酸分子的长度和/或在溶液中的浓度。
Description
PCT国内申请，说明书已公开。
Claims (17)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2018/116619 WO2020103012A1 (zh) | 2018-11-21 | 2018-11-21 | 核酸分子检测方法、检测装置和检测系统 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113166703A true CN113166703A (zh) | 2021-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201880099314.8A Pending CN113166703A (zh) | 2018-11-21 | 2018-11-21 | 核酸分子检测方法、检测装置和检测系统 |
Country Status (2)
| Country | Link |
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| CN (1) | CN113166703A (zh) |
| WO (1) | WO2020103012A1 (zh) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070281329A1 (en) * | 1995-03-17 | 2007-12-06 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| CN101605910A (zh) * | 2006-12-15 | 2009-12-16 | 帝国改革有限公司 | 电极系统及其在分子表征中的用途 |
| CN101680873A (zh) * | 2007-04-04 | 2010-03-24 | 加利福尼亚大学董事会 | 使用纳米孔的组合物、设备、系统和方法 |
| CN107002151A (zh) * | 2014-10-17 | 2017-08-01 | 牛津纳米孔技术公司 | 向跨膜孔输送分析物的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070202495A1 (en) * | 2006-02-06 | 2007-08-30 | Michael Mayer | Use of resistive-pulse sensing with submicrometer pores or nanopores for the detection of the assembly of submicrometer or nanometer sized objects |
| US10670559B2 (en) * | 2008-07-11 | 2020-06-02 | Cornell University | Nanofluidic channels with integrated charge sensors and methods based thereon |
| US8246799B2 (en) * | 2009-05-28 | 2012-08-21 | Nabsys, Inc. | Devices and methods for analyzing biomolecules and probes bound thereto |
| EP2580588B1 (en) * | 2010-06-08 | 2014-09-24 | President and Fellows of Harvard College | Nanopore device with graphene supported artificial lipid membrane |
| US9920361B2 (en) * | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| US9488614B2 (en) * | 2012-10-16 | 2016-11-08 | Abbott Laboratories | Localized desalting systems and methods |
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2018
- 2018-11-21 WO PCT/CN2018/116619 patent/WO2020103012A1/zh active Application Filing
- 2018-11-21 CN CN201880099314.8A patent/CN113166703A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070281329A1 (en) * | 1995-03-17 | 2007-12-06 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| CN101605910A (zh) * | 2006-12-15 | 2009-12-16 | 帝国改革有限公司 | 电极系统及其在分子表征中的用途 |
| CN101680873A (zh) * | 2007-04-04 | 2010-03-24 | 加利福尼亚大学董事会 | 使用纳米孔的组合物、设备、系统和方法 |
| CN107002151A (zh) * | 2014-10-17 | 2017-08-01 | 牛津纳米孔技术公司 | 向跨膜孔输送分析物的方法 |
Non-Patent Citations (1)
| Title |
|---|
| 余静文等: "基于微纳制造的下一代基因测序系统研究现状与展望", 中国科学 * |
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| WO2020103012A1 (zh) | 2020-05-28 |
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