JP2017526353A5 - - Google Patents

Download PDF

Info

Publication number
JP2017526353A5
JP2017526353A5 JP2017506386A JP2017506386A JP2017526353A5 JP 2017526353 A5 JP2017526353 A5 JP 2017526353A5 JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017526353 A5 JP2017526353 A5 JP 2017526353A5
Authority
JP
Japan
Prior art keywords
probe
sequence
item
nucleic acid
extension products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017506386A
Other languages
English (en)
Japanese (ja)
Other versions
JP6803327B2 (ja
JP2017526353A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/044065 external-priority patent/WO2016022833A1/en
Publication of JP2017526353A publication Critical patent/JP2017526353A/ja
Publication of JP2017526353A5 publication Critical patent/JP2017526353A5/ja
Application granted granted Critical
Publication of JP6803327B2 publication Critical patent/JP6803327B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017506386A 2014-08-06 2015-08-06 標的化されたシークエンシングからのデジタル測定値 Active JP6803327B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462034043P 2014-08-06 2014-08-06
US62/034,043 2014-08-06
PCT/US2015/044065 WO2016022833A1 (en) 2014-08-06 2015-08-06 Digital measurements from targeted sequencing

Publications (3)

Publication Number Publication Date
JP2017526353A JP2017526353A (ja) 2017-09-14
JP2017526353A5 true JP2017526353A5 (enExample) 2018-08-30
JP6803327B2 JP6803327B2 (ja) 2020-12-23

Family

ID=55264574

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017506386A Active JP6803327B2 (ja) 2014-08-06 2015-08-06 標的化されたシークエンシングからのデジタル測定値

Country Status (7)

Country Link
US (2) US10102337B2 (enExample)
EP (1) EP3177740B1 (enExample)
JP (1) JP6803327B2 (enExample)
CN (1) CN107075581B (enExample)
CA (1) CA2957633A1 (enExample)
SG (1) SG11201700891SA (enExample)
WO (1) WO2016022833A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3246416B1 (en) 2011-04-15 2024-06-05 The Johns Hopkins University Safe sequencing system
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014070462A1 (en) 2012-10-29 2014-05-08 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
CN105531375B (zh) 2012-12-10 2020-03-03 分析生物科学有限公司 靶向基因组分析的方法
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
EP3177740B1 (en) 2014-08-06 2021-01-13 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2018176007A1 (en) * 2017-03-23 2018-09-27 BioSpyder Technologies, Inc. Modulation of targets in cellular pathways identified by resolution of stochastic gene expression
CN107922970B (zh) 2015-08-06 2021-09-28 豪夫迈·罗氏有限公司 通过单探针引物延伸的靶标富集
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
WO2017210469A2 (en) 2016-06-01 2017-12-07 F. Hoffman-La Roche Ag Immuno-pete
CN106048009B (zh) * 2016-06-03 2020-02-18 人和未来生物科技（长沙）有限公司 一种用于超低频基因突变检测的标签接头及其应用
KR101969905B1 (ko) * 2016-06-20 2019-04-17 한국표준과학연구원 염기서열분석을 위한 라이브러리용 프라이머 세트 및 라이브러리 제조방법
KR102505122B1 (ko) * 2016-08-25 2023-03-08 레졸루션 바이오사이언스, 인크. Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
JP6970205B2 (ja) 2017-03-08 2021-11-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Ｄｎａおよびｒｎａの同時濃縮を含むプライマー伸長標的濃縮およびそれに対する向上
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
KR20250047324A (ko) 2017-08-07 2025-04-03 더 존스 홉킨스 유니버시티 암을 평가하고 치료하기 위한 방법 및 재료
US20200239932A1 (en) * 2017-10-16 2020-07-30 The Regents Of The University Of California Efficient screening library preparation
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
FI3707723T3 (fi) * 2017-11-06 2023-12-13 Illumina Inc Nukleiinihappojen indeksointitekniikoita
US10985407B2 (en) 2017-11-21 2021-04-20 Samsung Electronics Co., Ltd. All-solid-state secondary battery including anode active material alloyable with lithium and method of charging the same
EP3524695B1 (en) * 2018-02-13 2022-07-20 Personal Genomics S.r.l. Method for the enrichment of genomic regions
US20210310061A1 (en) * 2018-08-17 2021-10-07 Dana-Farber Cancer Institute, Inc. Dna amplification method for probe generation
CN110699426B (zh) * 2019-01-02 2022-01-28 上海臻迪基因科技有限公司 基因目标区域富集方法及试剂盒
CA3153256A1 (en) * 2019-09-06 2021-03-11 Honeycomb Biotechnologies, Inc. Methods and systems for rna-seq profiling
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
WO2021163546A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
CN113593636B (zh) * 2020-04-30 2024-05-03 深圳市真迈生物科技有限公司 测序结果分析方法、系统及计算机可读存储介质和电子设备
CN116075596A (zh) * 2020-08-07 2023-05-05 牛津纳米孔科技公开有限公司 鉴定核酸条形码的方法
WO2023066255A1 (zh) * 2021-10-18 2023-04-27 深圳市真迈生物科技有限公司 测序方法、测序数据处理方法、设备和计算机设备

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925065A (en) 1987-01-02 1990-05-15 Helena Laboratories Corporation Dispensing apparatus
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2013317A1 (en) 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
WO1992007951A1 (en) 1990-10-19 1992-05-14 Yoke Wah Kow Quantitation of aldehyde-containing lesions in nucleic acids
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
WO1993018052A1 (en) 1992-03-05 1993-09-16 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6110709A (en) 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
EP0832294A1 (en) 1995-06-07 1998-04-01 Pioneer Hi-Bred International, Inc. PCR-BASED cDNA SUBTRACTIVE CLONING METHOD
EP0843735B2 (en) 1995-07-11 2006-12-27 Forfas (trading as Bioresearch Ireland) Glycosylase mediated detection of known nucleotide sequences
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
GB9600384D0 (en) 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
WO1998006736A1 (en) 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
IL120337A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Method for joining DNA fragments
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
AU731069B2 (en) 1997-08-28 2001-03-22 Applied Biosystems, Llc Improved detection of mutations in nucleic acids by chemical cleavage
AR017023A1 (es) 1997-08-29 2001-08-22 Lopez Osvaldo J Tipeo genomico de adn metiltransferasa
WO1999022029A1 (en) 1997-10-28 1999-05-06 The Regents Of The University Of California Dna base mismatch detection using flow cytometry
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
DK1071811T3 (da) 1998-04-22 2002-06-17 Entpr Ie Trd As Bioresearch Ie Fremgangsmåde til karakterisering af nukleinsyremolekyler, hvilken fremgangsmåde involverer generering af forlængelige opstrøms-DNA-fragmenter, som er et resultat af spaltningen af nukleinsyre i et abasisk sted
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
GB9817055D0 (en) 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
AU2397400A (en) 1998-12-30 2000-07-31 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
WO2000055364A2 (en) 1999-03-12 2000-09-21 Amersham Pharmacia Biotech Uk Limited Genetic analysis
AU4739600A (en) 1999-05-17 2000-12-05 Mcgill University Method for subtracting cdnas by suppressing the synthesis of specifically targeted mrnas
US6225109B1 (en) 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
ATE262595T1 (de) 1999-09-13 2004-04-15 Nugen Technologies Inc Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6617442B1 (en) 1999-09-30 2003-09-09 Isis Pharmaceuticals, Inc. Human Rnase H1 and oligonucleotide compositions thereof
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
AU2843201A (en) 1999-12-21 2001-07-03 Vbc-Genomics Bioscience Research Gmbh Compound comprising a peptide moiety and an organo-silane moiety.
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
EP1356094B1 (en) 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
AU2002213096B2 (en) 2000-10-06 2007-03-29 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6893820B1 (en) 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
EP1390537B1 (en) 2001-03-09 2013-11-13 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
WO2002081753A1 (en) 2001-04-04 2002-10-17 Advanced Research & Technology Institute Method for identifying and characterizing individual dna molecules
FR2824335A1 (fr) 2001-05-04 2002-11-08 Bio Merieux Procede de marquage et de fragmentation d'adn
EP1404825A2 (en) 2001-06-27 2004-04-07 Roche Diagnostics GmbH A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES
AU2002341891A1 (en) 2001-09-26 2003-04-07 Epigenx Pharmaceuticals, Inc. Assays for dna methylation changes
JP2004148509A (ja) 2001-10-04 2004-05-27 Seiko Epson Corp 液体噴射ヘッド
WO2003078645A2 (en) 2002-03-11 2003-09-25 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3’ single stranded portion and uses of these complexes for recombination
EP1490514A4 (en) 2002-03-29 2008-12-17 Nugen Technologies Inc THROUGH ISOTHERME SINGLE PRIMER NUCLEIC ACID AMPLIFICATION IMPROVED DETECTION AND QUANTIFICATION OF ANALYTES
US6887709B2 (en) 2002-05-09 2005-05-03 Lifescan, Inc. Devices, systems and methods for the containment and use of liquid solutions
WO2004011665A2 (en) 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
WO2005038427A2 (en) 2003-06-05 2005-04-28 Medical Gene Center Ltd. Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7361468B2 (en) 2004-07-02 2008-04-22 Affymetrix, Inc. Methods for genotyping polymorphisms in humans
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
JP2008520975A (ja) 2004-11-16 2008-06-19 ヘリコス バイオサイエンシーズ コーポレイション Ｔｉｒｆ単分子分析および核酸を配列決定する方法
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7462468B1 (en) 2005-01-28 2008-12-09 Pacific Biosciences Of California, Inc. DNA intercalating agents and methods of use
AU2006211150A1 (en) 2005-01-31 2006-08-10 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
US20060286566A1 (en) 2005-02-03 2006-12-21 Helicos Biosciences Corporation Detecting apparent mutations in nucleic acid sequences
US20060240451A1 (en) 2005-02-09 2006-10-26 Epicentre Technologies Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases
DK1924704T3 (da) 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
US20090137415A1 (en) 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1957667A1 (en) 2005-11-15 2008-08-20 Solexa Ltd. Method of target enrichment
EP2010657A2 (en) 2006-04-24 2009-01-07 Nimblegen Systems, Inc. Use of microarrays for genomic representation selection
US20080269476A1 (en) 2006-04-26 2008-10-30 Helicos Biosciences Corporation Molecules and methods for nucleic acid sequencing
US20090275486A1 (en) 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
WO2008005459A2 (en) 2006-06-30 2008-01-10 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
WO2008033442A2 (en) 2006-09-12 2008-03-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2008112653A1 (en) 2007-03-09 2008-09-18 Dxtech, Llc Electrochemical detection system
US7767805B2 (en) 2007-05-03 2010-08-03 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US9248076B2 (en) 2007-05-16 2016-02-02 Mystic Pharmaceuticals, Inc. Dose dispensing containers
US8182993B2 (en) 2007-06-06 2012-05-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CL2008001682A1 (es) 2007-06-08 2008-12-12 Monsanto Technology Llc Metodos para el mejoramiento de plantas mediante el uso de informacion directa de secuencias de acidos nucleicos.
WO2009017678A2 (en) 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
EP2247727A4 (en) 2008-02-12 2011-08-03 Nugen Technologies Inc PROCESS FOR ARCHIVING AND CLONING EXPANSION
GB0804690D0 (en) 2008-03-13 2008-04-16 Netherlands Cancer Inst The Method
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2010003153A2 (en) 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
JP5221248B2 (ja) 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のｃＤＮＡクローンの含有率を低減させたｃＤＮＡライブラリーの作製方法
WO2010030683A1 (en) 2008-09-09 2010-03-18 Rosetta Inpharmatics Llc Methods of generating gene specific libraries
WO2010039991A2 (en) 2008-10-02 2010-04-08 The Texas A&M University System Method of generating informative dna templates for high-throughput sequencing applications
EP2367937B1 (en) 2008-12-04 2013-04-17 Keygene N.V. Method for the reduction of repetitive sequences in adapter-ligated restriction fragments
GB2465986A (en) 2008-12-04 2010-06-09 Angeletti P Ist Richerche Bio Method of generating diversity in polynucleotide sequences
WO2010085715A1 (en) 2009-01-22 2010-07-29 Quanta Biosciences Method for enrichment of selected rna molecules
US20120107811A1 (en) 2009-02-06 2012-05-03 Kelso David M Burstable liquid packaging and uses thereof
CN103952482A (zh) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 用于对目标核酸进行条形码化的多引物扩增方法
US20110003301A1 (en) 2009-05-08 2011-01-06 Life Technologies Corporation Methods for detecting genetic variations in dna samples
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
EP2464738A4 (en) 2009-08-12 2013-05-01 Nugen Technologies Inc METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF MATRIX NUCLEIC ACID
WO2011032053A1 (en) 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
GB2487341A (en) 2009-11-02 2012-07-18 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence selection and amplification
ITRM20100293A1 (it) 2010-05-31 2011-12-01 Consiglio Nazionale Ricerche Metodo per la preparazione e amplificazione di librerie rappresentative di cdna per il sequenziamento massivo, loro uso, kit e cartucce per kit di automazione
WO2011156529A2 (en) 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
GB201012748D0 (en) 2010-07-29 2010-09-15 Univ St Andrews Improved RACE
EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
DK2630263T4 (da) 2010-10-22 2022-02-14 Cold Spring Harbor Laboratory Varital tælling af nucleinsyrer for at opnå information om antal genomiske kopier
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
CN105861487B (zh) * 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US8209130B1 (en) * 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
HK1212401A1 (en) * 2012-08-15 2016-06-10 Natera, Inc. Methods and compositions for reducing genetic library contamination
PL2893040T3 (pl) 2012-09-04 2019-05-31 Guardant Health Inc Sposoby wykrywania rzadkich mutacji i wariantu liczby kopii
EP3351661B1 (en) 2012-11-26 2025-05-21 The University of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
WO2015057899A1 (en) 2013-10-15 2015-04-23 Mystic Pharmaceuticals, Inc. Controllable rate turbulating nozzle
JP6525473B2 (ja) * 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
GB201320542D0 (en) 2013-11-21 2014-01-01 Randox Lab Ltd Assay fluid delivery system
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
EP3177740B1 (en) 2014-08-06 2021-01-13 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US9527077B2 (en) 2015-01-29 2016-12-27 David W. Wright Diagnostic cartridge, fluid storage and delivery apparatus therefor and methods of construction thereof

Similar Documents

Publication Publication Date Title
US11795501B2 (en) Methods for next generation genome walking and related compositions and kits
Sengupta et al. Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization
Shirima et al. Absolute quantification of cassava brown streak virus mRNA by real-time qPCR
JP5992911B2 (ja) 分子計数による対立遺伝子呼び出しの信頼度の増加
CN107075581B (zh) 由靶向测序进行数字测量
No et al. Comparison of targeted next-generation sequencing for whole-genome sequencing of Hantaan orthohantavirus in Apodemus agrarius lung tissues
CN111020062A (zh) 一种检测非洲猪瘟野毒株和基因缺失株的三重实时荧光定量pcr试剂盒
JP2013531983A (ja) 多重生物検出のための核酸ならびにその使用および製造方法
Pamponét et al. Low coverage sequencing for repetitive DNA analysis in Passiflora edulis Sims: citogenomic characterization of transposable elements and satellite DNA
KR102242192B1 (ko) 미세 조정된 초특정 핵산 하이브리다이제이션 프로브
Malandraki et al. Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
US20080261198A1 (en) Diagnostic Primers and Method for Detecting Avian Influenza Virus Subtype H5 and H5n1
JP7801240B2 (ja) 病原体診断検査
JP7528911B2 (ja) Ｄｎａライブラリーの作製方法及びｄｎａライブラリーを用いたゲノムｄｎａ解析方法
CN108517371B (zh) 用于二倍体d基因组棉种或四倍体棉种鉴定的引物及方法
CN102719543B (zh) 利用核苷酸化学分子式鉴定植物品种的方法
Xiao et al. Design and validation of a universal influenza virus enrichment probe set and its utility in deep sequence analysis of primary cloacal swab surveillance samples of wild birds
Chen et al. Reproducibility, fidelity, and discriminant validity of linear RNA amplification for microarray-based identification of major human enteric viruses
CN106967833B (zh) 用于二倍体a基因组棉种和/或四倍体棉种鉴定的引物及其pcr鉴定方法
CN107142321B (zh) 一种蒙古扁桃特异性微卫星位点及其应用
US20200071776A1 (en) Set of random primers and method for preparing dna library using the same
Wang et al. Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2
CN110195117A (zh) 检测分枝杆菌的方法及其试剂盒
CN118879945B (zh) 一种疫苗毒株鉴定的引物探针组合、试剂盒及应用
Mears et al. Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP