JP2017526353A5 - - Google Patents

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JP2017526353A5
JP2017526353A5 JP2017506386A JP2017506386A JP2017526353A5 JP 2017526353 A5 JP2017526353 A5 JP 2017526353A5 JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017506386 A JP2017506386 A JP 2017506386A JP 2017526353 A5 JP2017526353 A5 JP 2017526353A5
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probe
sequence
item
nucleic acid
extension products
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JP2017506386A 2014-08-06 2015-08-06 標的化されたシークエンシングからのデジタル測定値 Active JP6803327B2 (ja)

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US201462034043P 2014-08-06 2014-08-06
US62/034,043 2014-08-06
PCT/US2015/044065 WO2016022833A1 (en) 2014-08-06 2015-08-06 Digital measurements from targeted sequencing

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JP2017526353A JP2017526353A (ja) 2017-09-14
JP2017526353A5 true JP2017526353A5 (enExample) 2018-08-30
JP6803327B2 JP6803327B2 (ja) 2020-12-23

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US (2) US10102337B2 (enExample)
EP (1) EP3177740B1 (enExample)
JP (1) JP6803327B2 (enExample)
CN (1) CN107075581B (enExample)
CA (1) CA2957633A1 (enExample)
SG (1) SG11201700891SA (enExample)
WO (1) WO2016022833A1 (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3246416T3 (da) 2011-04-15 2024-09-02 Univ Johns Hopkins Sikkert sekventeringssystem
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
ES2886507T5 (es) 2012-10-29 2024-11-15 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2018176007A1 (en) * 2017-03-23 2018-09-27 BioSpyder Technologies, Inc. Modulation of targets in cellular pathways identified by resolution of stochastic gene expression
WO2017021449A1 (en) 2015-08-06 2017-02-09 F. Hoffmann-La Roche Ag Target enrichment by single probe primer extension
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
EP3464629B1 (en) 2016-06-01 2021-09-08 F. Hoffmann-La Roche AG Immuno-pete
CN106048009B (zh) * 2016-06-03 2020-02-18 人和未来生物科技（长沙）有限公司 一种用于超低频基因突变检测的标签接头及其应用
KR101969905B1 (ko) * 2016-06-20 2019-04-17 한국표준과학연구원 염기서열분석을 위한 라이브러리용 프라이머 세트 및 라이브러리 제조방법
AU2017315769B2 (en) * 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
EP3592863A1 (en) 2017-03-08 2020-01-15 H. Hoffnabb-La Roche Ag Primer extension target enrichment and improvements thereto including simultaneous enrichment of dna and rna
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
CN111868260B (zh) 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
WO2019078909A2 (en) * 2017-10-16 2019-04-25 The Regents Of The University Of California PREPARATION OF EFFECTIVE SCREENING LIBRARY
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN118126816A (zh) 2017-11-06 2024-06-04 伊鲁米那股份有限公司 核酸索引化技术
JP2019096610A (ja) 2017-11-21 2019-06-20 三星電子株式会社Ｓａｍｓｕｎｇ Ｅｌｅｃｔｒｏｎｉｃｓ Ｃｏ．，Ｌｔｄ． 全固体二次電池およびその充電方法
EP3524695B1 (en) * 2018-02-13 2022-07-20 Personal Genomics S.r.l. Method for the enrichment of genomic regions
CA3106677A1 (en) * 2018-08-17 2020-02-20 Dana-Farber Cancer Institue, Inc. Dna amplification method for probe generation
CN110699426B (zh) * 2019-01-02 2022-01-28 上海臻迪基因科技有限公司 基因目标区域富集方法及试剂盒
AU2020341808A1 (en) * 2019-09-06 2022-03-24 Honeycomb Biotechnologies, Inc. Methods and systems for RNA-seq profiling
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
CN115516108A (zh) 2020-02-14 2022-12-23 约翰斯霍普金斯大学 评估核酸的方法和材料
CN113593636B (zh) * 2020-04-30 2024-05-03 深圳市真迈生物科技有限公司 测序结果分析方法、系统及计算机可读存储介质和电子设备
US20220059187A1 (en) * 2020-08-07 2022-02-24 Oxford Nanopore Technologies Limited Methods of detecting nucleic acid barcodes
CN118139990A (zh) * 2021-10-18 2024-06-04 深圳市真迈生物科技有限公司 测序方法、测序数据处理方法、设备和计算机设备

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925065A (en) 1987-01-02 1990-05-15 Helena Laboratories Corporation Dispensing apparatus
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
CA2013317A1 (en) 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
WO1992007951A1 (en) 1990-10-19 1992-05-14 Yoke Wah Kow Quantitation of aldehyde-containing lesions in nucleic acids
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
EP0635023B1 (en) 1992-03-05 2002-02-06 Isis Pharmaceuticals, Inc. Covalently cross-linked oligonucleotides
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US6110709A (en) 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
AU700952B2 (en) 1995-06-07 1999-01-14 Pioneer Hi-Bred International, Inc. PCR-based cDNA subtractive cloning method
ES2176358T3 (es) 1995-07-11 2002-12-01 Forfas Trading As Biores Irela Deteccion mediada por glucosilasa de secuencias nucleotidicas conocidas.
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
GB9600384D0 (en) 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases
EP2264045B1 (en) 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
IL120337A0 (en) 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Method for joining DNA fragments
ATE364718T1 (de) 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
JP2002511767A (ja) 1997-08-28 2002-04-16 ピーイー コーポレイション（エヌワイ） 化学切断による、核酸における変異の改良された検出
AR017023A1 (es) 1997-08-29 2001-08-22 Lopez Osvaldo J Tipeo genomico de adn metiltransferasa
IL135852A0 (en) 1997-10-28 2001-05-20 Univ California A method for detecting dna base mismatch
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
ATE214104T1 (de) 1998-04-22 2002-03-15 Entpr Ie Trd As Bioresearch Ie Verfahren zur charakterisierung von nukleinsäuremolekulen, das die erzeugung von verlängerbaren aufwärtsgelegenen dns-fragmenten, die durch spaltung der nukleinsäure an einer abasischen stelle entsteht, umfasst
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
GB9817055D0 (en) 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CA2357601A1 (en) 1998-12-30 2000-07-06 Dana-Farber Cancer Institute, Inc. Mutation scanning array, and methods of use thereof
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
JP2002538837A (ja) 1999-03-12 2002-11-19 アマシャム バイオサイエンス ユーケイ リミテッド 遺伝分析
WO2000070039A1 (en) 1999-05-17 2000-11-23 Mcgill University METHOD FOR SUBTRACTING cDNAs BY SUPPRESSING THE SYNTHESIS OF SPECIFICALLY TARGETED mRNAs
US6225109B1 (en) 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
IL148091A0 (en) 1999-09-13 2002-09-12 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6617442B1 (en) 1999-09-30 2003-09-09 Isis Pharmaceuticals, Inc. Human Rnase H1 and oligonucleotide compositions thereof
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
EP1244812A1 (en) 1999-12-21 2002-10-02 LION Bioscience AG Branched compound for use in nucleic acid detection and analysis reactions
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
DE60141087D1 (de) 2000-06-26 2010-03-04 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
CA2423729A1 (en) 2000-10-06 2002-04-11 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
IE20000887A1 (en) 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6893820B1 (en) 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
CA2439074A1 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
WO2002072772A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
WO2002081753A1 (en) 2001-04-04 2002-10-17 Advanced Research & Technology Institute Method for identifying and characterizing individual dna molecules
FR2824335A1 (fr) 2001-05-04 2002-11-08 Bio Merieux Procede de marquage et de fragmentation d'adn
JP3967319B2 (ja) 2001-06-27 2007-08-29 エフ．ホフマン−ラ ロシュ アーゲー ポリヌクレオチド配列のインビトロ組換えのためのウォークスルー技術
EP1438416A4 (en) 2001-09-26 2007-09-05 Epigenx Pharmaceutical Inc ASSAYS FOR CHANGES TO THE METHYLATION OF DNA
JP2004148509A (ja) 2001-10-04 2004-05-27 Seiko Epson Corp 液体噴射ヘッド
CA2478875A1 (en) 2002-03-11 2003-09-25 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
AU2003222178A1 (en) 2002-03-29 2003-10-13 Nugen Technologies, Inc. Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification
US6887709B2 (en) 2002-05-09 2005-05-03 Lifescan, Inc. Devices, systems and methods for the containment and use of liquid solutions
AU2003279697A1 (en) 2002-05-17 2004-02-16 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilizaton of nucleic acids
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
WO2005038427A2 (en) * 2003-06-05 2005-04-28 Medical Gene Center Ltd. Rapid identification of viruses of the upper respiratory tract infection including sars causing coronus viruses
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20050208538A1 (en) 2003-12-29 2005-09-22 Nurith Kurn Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7361468B2 (en) 2004-07-02 2008-04-22 Affymetrix, Inc. Methods for genotyping polymorphisms in humans
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7276720B2 (en) 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
US20060024678A1 (en) 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
CA2588122A1 (en) 2004-11-16 2006-05-26 Helicos Biosciences Corporation Tirf single molecule analysis and method of sequencing nucleic acids
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7462468B1 (en) 2005-01-28 2008-12-09 Pacific Biosciences Of California, Inc. DNA intercalating agents and methods of use
US7476504B2 (en) 2005-01-31 2009-01-13 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
US20060286566A1 (en) 2005-02-03 2006-12-21 Helicos Biosciences Corporation Detecting apparent mutations in nucleic acid sequences
WO2006086668A2 (en) 2005-02-09 2006-08-17 Epicentre Technologies Compositions and methods employing 5'-phosphate-dependent nucleic acid exonucleases
EP1924704B1 (en) 2005-08-02 2011-05-25 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
US20090137415A1 (en) 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
WO2007057652A1 (en) 2005-11-15 2007-05-24 Solexa Limited Method of target enrichment
WO2008115185A2 (en) 2006-04-24 2008-09-25 Nimblegen Systems, Inc. Use of microarrays for genomic representation selection
US20080269476A1 (en) 2006-04-26 2008-10-30 Helicos Biosciences Corporation Molecules and methods for nucleic acid sequencing
WO2007136717A1 (en) 2006-05-16 2007-11-29 Nugen Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
EP2038429B1 (en) 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US8293501B2 (en) 2006-09-12 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8506908B2 (en) 2007-03-09 2013-08-13 Vantix Holdings Limited Electrochemical detection system
US7767805B2 (en) 2007-05-03 2010-08-03 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US9248076B2 (en) 2007-05-16 2016-02-02 Mystic Pharmaceuticals, Inc. Dose dispensing containers
CA2689626C (en) 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
AR066922A1 (es) * 2007-06-08 2009-09-23 Monsanto Technology Llc Metodos de mejoramiento molecular del germoplasma de una planta por secuenciamiento dirigido
JP2010534474A (ja) 2007-07-26 2010-11-11 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 分子冗長配列決定
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
EP2247727A4 (en) 2008-02-12 2011-08-03 Nugen Technologies Inc PROCESS FOR ARCHIVING AND CLONING EXPANSION
GB0804690D0 (en) 2008-03-13 2008-04-16 Netherlands Cancer Inst The Method
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
CN102084001B (zh) 2008-03-28 2015-03-18 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US20100120034A1 (en) 2008-07-03 2010-05-13 Life Technologies Corporation Methylation analysis of mate pairs
JP5221248B2 (ja) 2008-08-26 2013-06-26 株式会社日立ハイテクノロジーズ 高発現遺伝子由来のｃＤＮＡクローンの含有率を低減させたｃＤＮＡライブラリーの作製方法
EP2334802A4 (en) 2008-09-09 2012-01-25 Life Technologies Corp METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES
WO2010039991A2 (en) 2008-10-02 2010-04-08 The Texas A&M University System Method of generating informative dna templates for high-throughput sequencing applications
EP2367937B1 (en) 2008-12-04 2013-04-17 Keygene N.V. Method for the reduction of repetitive sequences in adapter-ligated restriction fragments
GB2465986A (en) 2008-12-04 2010-06-09 Angeletti P Ist Richerche Bio Method of generating diversity in polynucleotide sequences
WO2010085715A1 (en) 2009-01-22 2010-07-29 Quanta Biosciences Method for enrichment of selected rna molecules
WO2010091246A2 (en) 2009-02-06 2010-08-12 Northwestern University Burstable liquid packaging and uses thereof
EP2414547B1 (en) 2009-04-02 2014-03-12 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US20110003301A1 (en) 2009-05-08 2011-01-06 Life Technologies Corporation Methods for detecting genetic variations in dna samples
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
GB0912909D0 (en) 2009-07-23 2009-08-26 Olink Genomics Ab Probes for specific analysis of nucleic acids
EP2464738A4 (en) 2009-08-12 2013-05-01 Nugen Technologies Inc METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF MATRIX NUCLEIC ACID
WO2011032053A1 (en) 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
WO2011053987A1 (en) 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence selection and amplification
ITRM20100293A1 (it) 2010-05-31 2011-12-01 Consiglio Nazionale Ricerche Metodo per la preparazione e amplificazione di librerie rappresentative di cdna per il sequenziamento massivo, loro uso, kit e cartucce per kit di automazione
CN103119439A (zh) 2010-06-08 2013-05-22 纽亘技术公司 用于多重测序的方法和组合物
GB201012748D0 (en) 2010-07-29 2010-09-15 Univ St Andrews Improved RACE
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
HUE039766T2 (hu) 2010-10-22 2019-02-28 Cold Spring Harbor Laboratory Nukleinsavak változatainak megszámlálása genom kópiaszám információ megszerzésére
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
WO2013138510A1 (en) 2012-03-13 2013-09-19 Patel Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US8209130B1 (en) * 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US11261494B2 (en) * 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
EP2885445A4 (en) * 2012-08-15 2016-04-13 Natera Inc METHOD AND COMPOSITIONS FOR CONTAMINATING REDUCTION OF GENETIC LIBRARIES
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
WO2014082032A1 (en) * 2012-11-26 2014-05-30 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
CN110964796B (zh) 2013-08-28 2024-04-05 贝克顿迪金森公司 大规模平行单细胞分析
CA2936301C (en) 2013-10-15 2021-06-08 Mystic Pharmaceuticals, Inc. Controllable rate turbulating nozzle
WO2015073711A1 (en) * 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
GB201320542D0 (en) 2013-11-21 2014-01-01 Randox Lab Ltd Assay fluid delivery system
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US9527077B2 (en) 2015-01-29 2016-12-27 David W. Wright Diagnostic cartridge, fluid storage and delivery apparatus therefor and methods of construction thereof

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