DK1071811T3 - Fremgangsmåde til karakterisering af nukleinsyremolekyler, hvilken fremgangsmåde involverer generering af forlængelige opstrøms-DNA-fragmenter, som er et resultat af spaltningen af nukleinsyre i et abasisk sted - Google Patents

Fremgangsmåde til karakterisering af nukleinsyremolekyler, hvilken fremgangsmåde involverer generering af forlængelige opstrøms-DNA-fragmenter, som er et resultat af spaltningen af nukleinsyre i et abasisk sted

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Publication number
DK1071811T3
DK1071811T3 DK98917568T DK98917568T DK1071811T3 DK 1071811 T3 DK1071811 T3 DK 1071811T3 DK 98917568 T DK98917568 T DK 98917568T DK 98917568 T DK98917568 T DK 98917568T DK 1071811 T3 DK1071811 T3 DK 1071811T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
abasic site
cleavage
acid molecules
dna
Prior art date
Application number
DK98917568T
Other languages
English (en)
Inventor
Thomas Valentine Mccarthy
Patrick Martin Vaughan
Original Assignee
Entpr Ie Trd As Bioresearch Ie
Univ College Cork Nat Univ Ie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Entpr Ie Trd As Bioresearch Ie, Univ College Cork Nat Univ Ie filed Critical Entpr Ie Trd As Bioresearch Ie
Application granted granted Critical
Publication of DK1071811T3 publication Critical patent/DK1071811T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DK98917568T 1998-04-22 1998-04-22 Fremgangsmåde til karakterisering af nukleinsyremolekyler, hvilken fremgangsmåde involverer generering af forlængelige opstrøms-DNA-fragmenter, som er et resultat af spaltningen af nukleinsyre i et abasisk sted DK1071811T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IE1998/000030 WO1999054501A1 (en) 1998-04-22 1998-04-22 A method for the characterisation of nucleic acid molecules involving generation of extendible upstream dna fragments resulting from the cleavage of nucleic acid at an abasic site

Publications (1)

Publication Number Publication Date
DK1071811T3 true DK1071811T3 (da) 2002-06-17

Family

ID=11042510

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98917568T DK1071811T3 (da) 1998-04-22 1998-04-22 Fremgangsmåde til karakterisering af nukleinsyremolekyler, hvilken fremgangsmåde involverer generering af forlængelige opstrøms-DNA-fragmenter, som er et resultat af spaltningen af nukleinsyre i et abasisk sted

Country Status (14)

Country Link
US (1) US7175982B1 (da)
EP (1) EP1071811B8 (da)
JP (1) JP2002512044A (da)
CN (1) CN1198943C (da)
AT (1) ATE214104T1 (da)
AU (1) AU753661B2 (da)
CA (1) CA2325854C (da)
DE (1) DE69804143T2 (da)
DK (1) DK1071811T3 (da)
ES (1) ES2172887T3 (da)
NO (1) NO20005311L (da)
NZ (1) NZ508358A (da)
PT (1) PT1071811E (da)
WO (1) WO1999054501A1 (da)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001027857A2 (en) 1999-10-13 2001-04-19 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
IE20000887A1 (en) * 2000-11-03 2002-12-11 Univ College Cork Nat Univ Ie Method for the amplification and optional characterisation of nucleic acids
GB0126887D0 (en) 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP2290096B1 (en) 2002-02-21 2014-11-19 Alere San Diego, Inc. Recombinase polymerase amplification using a temperature-sensitive recombinase agent
JP4663988B2 (ja) * 2002-04-12 2011-04-06 ニュー・イングランド・バイオラブズ・インコーポレイティッド Ｄｎａ操作のための方法および組成物
IE20020544A1 (en) * 2002-06-28 2003-12-31 Univ College Cork Nat Univ Ie Method for the characterisation of nucleic acid molecules
WO2004018626A2 (en) 2002-08-21 2004-03-04 Epoch Biosciences, Inc. Abasic site endonuclease assay
JP4786904B2 (ja) 2002-11-27 2011-10-05 セクエノム，インコーポレイティド 配列変化検出及び発見用の断片化をベースとする方法及びシステム
EP1647592B1 (en) * 2003-06-30 2009-03-11 Panasonic Corporation Method of modifying nucleotide chain
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
AU2005230936B2 (en) 2004-03-26 2010-08-05 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
JP2006271311A (ja) * 2005-03-30 2006-10-12 Hitachi Ltd 核酸分析方法
CA2616241C (en) * 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
EP3088533B1 (en) 2006-05-04 2018-01-17 Alere San Diego, Inc. Recombinase polymerase amplification
CA2687218A1 (en) * 2007-05-14 2008-11-27 Insight Genetics, Inc. Methods of screening nucleic acids for single nucleotide variations
US20100285537A1 (en) * 2009-04-02 2010-11-11 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
BR112012006226A2 (pt) 2009-09-23 2020-04-07 Koninl Philips Electronics Nv método para monitorar a conversão de dna mediada por bissulfito durante a análise de metilação do dna, uso de um método e uso de uma molécula reporter
ES2578370T3 (es) * 2011-07-01 2016-07-26 HTG Molecular Diagnostics, Inc Métodos para detectar fusiones génicas
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
WO2013112923A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP2861787B1 (en) 2012-06-18 2017-09-20 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
WO2015091525A1 (en) * 2013-12-16 2015-06-25 Syddansk Universitet Ras exon 2 skipping for cancer treatment
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
SG11201700891SA (en) 2014-08-06 2017-03-30 Nugen Technologies Inc Digital measurements from targeted sequencing
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996030545A1 (en) * 1995-03-24 1996-10-03 Mitokor Mutation detection by differential primer extension of mutant and wildtype target sequences
US5656430A (en) * 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
NZ298494A (en) * 1995-07-11 1999-09-29 Forfas Trading As Bioresearch Glycosylase mediated detection of nucleotide sequences at candidate loci
US6190865B1 (en) 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules

Also Published As

Publication number Publication date
EP1071811A1 (en) 2001-01-31
EP1071811B8 (en) 2003-01-02
NO20005311L (no) 2000-12-21
ATE214104T1 (de) 2002-03-15
ES2172887T3 (es) 2002-10-01
DE69804143D1 (de) 2002-04-11
US7175982B1 (en) 2007-02-13
NO20005311D0 (no) 2000-10-20
PT1071811E (pt) 2002-08-30
AU753661B2 (en) 2002-10-24
EP1071811B1 (en) 2002-03-06
JP2002512044A (ja) 2002-04-23
WO1999054501A1 (en) 1999-10-28
AU7075098A (en) 1999-11-08
CN1198943C (zh) 2005-04-27
CA2325854A1 (en) 1999-10-28
CA2325854C (en) 2005-12-06
CN1291240A (zh) 2001-04-11
NZ508358A (en) 2002-07-26
DE69804143T2 (de) 2002-08-01

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