JP2017503506A - インターロイキン−33(il−33)に対する抗体 - Google Patents
インターロイキン−33(il−33)に対する抗体 Download PDFInfo
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Abstract
Description
本明細書と同時提出され以下のとおり特定される、コンピューターで読み取り可能なヌクレオチド/アミノ酸配列表は、参照によりその全体が本明細書に組み込まれる:283,358バイトのASCII(テキスト)ファイル1件、名称「719408_ST25.txt」、2015年1月8日作成。
インターロイキン33(IL-33)は、高内皮細静脈における核内因子(nuclear factor(NF)in high endothelial venules)(NF-HEV)としても知られ、IL-1スーパーファミリーに属するサイトカインである。IL-33は、2型サイトカインを産生するようヘルパーT細胞、マスト細胞、好酸球及び好塩基球を誘導する。IL-33は、受容体ST2(IL1RL1としても知られる)及びIL-1受容体アクセサリータンパク質(IL-1 Receptor Accessory Protein、IL1RAP)と相互作用することによりその生物学的効果を媒介しており、NF-κB及びMAPキナーゼシグナリング経路における細胞内分子を活性化し、皮膚、肺及び消化管において、極性化したヘルパーT細胞(Th2)及びグループ2自然リンパ球(Group-2 innate lymphoid cells、ILC2)からの2型サイトカイン(例えば、IL-4、IL-5及びIL-13)の産生を推進する。IL-33は、マスト細胞に直接作用してその活性化を引き起こし、好酸球及び好塩基球を刺激して脱顆粒させ、組織損傷を引き起こす。IL-33によるin vivoでの2型サイトカインの誘導は、IL-33投与後に粘膜器官で観察される重篤な病理学的変化を誘導すると考えられる(例えば、Schmitz et al., Immunity, 23(5):479-490(2005);及び Chackerian et al., J. Immunol., 179(4):2551-2555(2007)を参照)。
本発明は、単離された免疫グロブリン重鎖ポリペプチドであって、(a)配列番号1、配列番号2及び配列番号5〜50のいずれか1つのアミノ酸配列、又は(b)配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つと少なくとも90%同一であるアミノ酸配列を含む、単離された免疫グロブリン重鎖ポリペプチドを提供する。
本発明は、単離された免疫グロブリン重鎖ポリペプチド及び/若しくは単離された免疫グロブリン軽鎖ポリペプチド、又はそのフラグメント(例えば、抗原結合フラグメント)を提供する。本明細書で使用する場合、用語「免疫グロブリン」又は「抗体」とは、脊椎動物の血液又は他の体液中で見られるタンパク質をいい、細菌及びウイルスなどの異物を特定し中和するために免疫系によって使用される。ポリペプチドは、その天然環境から取り出されるという点で「単離される」。好ましい実施態様において、免疫グロブリン又は抗体は、少なくとも1つの相補性決定領域(CDR)を含むタンパク質である。CDRsは、抗体の「超可変領域」を形成し、これは抗原結合に関与する(以下にさらに記載する)。免疫グロブリン全体は、典型的には、4つのポリペプチドからなる:重(H)鎖ポリペプチドの2つの同一コピー及び軽(L)鎖ポリペプチドの2つの同一コピー。重鎖の各々は、1つのN末端可変(VH)領域及び3つのC末端定常(CH1、CH2及びCH3)領域を含み、各軽鎖は、1つのN末端可変(VL)領域及び1つのC末端定常(CL)領域を含む。抗体の軽鎖は、それらの定常ドメインのアミノ酸配列に基づいて、2つの別個のタイプのうちの1つ(カッパ(κ)又はラムダ(λ)のいずれか)に割り当てられ得る。典型的な免疫グロブリンにおいて、各軽鎖は、ジスルフィド結合により重鎖と連結され、2つの重鎖は、ジスルフィド結合により互いに連結される。軽鎖可変領域は、重鎖の可変領域と整列し、軽鎖定常領域は、重鎖の第1の定常領域と整列する。重鎖の残りの定常領域は、互いに整列する。
本実施例は、本発明の免疫グロブリン重鎖及び軽鎖ポリペプチドの機能活性を決定するために使用されるアッセイを記述する。
KU812細胞(ヒト好塩基球様(basophil-like)CML細胞株(ATCC No. CRL-2099))(例えば、Tare et al., Exp. Cell Res., 316(15):2527-37(2010);Lefrancais et al., Proc. Natl. Acad. Sci. USA, 109(5):1673-1978(2012)を参照)は、IL-5を分泌することによりIL-33刺激に反応する。KU812細胞をRPM1+10%FBS培養培地に懸濁し、ウェルあたり500,000細胞を96ウェル平底プレートへとプレーティングした。目的の抗体について30 μg/mLのストックを段階希釈し、half-log間隔で8つの濃度を作製した。希釈サンプルを、細胞に列で添加し、37℃、30分間インキュベートした。次いで、IL-33-his6-bio(6 HisでC末端標識され、分子あたり平均1〜2のビオチンでビオチン化される)(3 ng)を各ウェルに添加し、プレートを37℃、48時間インキュベートした。次いで、上清を取り出し、ELISAによる試験まで4℃で保持した。IL-5 DuoSet ELISAキット(R&D Systems, Minneapolis, MN)を用いて上清を試験し、SOFTMAX PROTM Microplate Data Acquisition & Analysis Software(Molecular Devices, LLC, Sunnyvale, CA)を用いて、SPECTRAMAXTMマイクロプレートリーダー(Molecular Devices, LLC, Sunnyvale, CA)にて評価して、IL-5産生を決定した。
HEK/ST2安定細胞株は、ナイーブ(naive)なHEK細胞をDMEM/10%FBS中、3×103細胞/T75フラスコで最初にプレーティングし、37℃、一晩インキュベートすることにより作製した。翌日、500 μL Optimem(Life Technologies, Carlsbad, CA)+24 μL HD FUGENETM(Promega, Madison, WI)を混合することにより細胞をトランスフェクトし、室温で5分間インキュベートさせた。ST2-FcをコードするDNA(4 μg)をFUGENETM混合物に添加し、室温で25分間インキュベートさせた。次いで、DNA/FUGENETM混合物をHEK細胞上に供給(distributed)し、37℃、5%CO2で一晩インキュベートさせた。トランスフェクション後24時間にて、細胞を分割(split)し、安定的に選択されるまで、3〜4週間の期間、ハイグロマイシン選択下に置いた。
4×106 HEK293/ST2-Fc細胞をT-75フラスコにて37℃、5%CO2で一晩播種した。翌朝、ルシフェラーゼレポーター遺伝子の発現を惹起(driving)するヒトIL-8プロモーターをコードするDNAコンストラクトAB4111を、500 μL Optimem+24 μL HD FUGENETMを混合することにより細胞へとトランスフェクトし、室温で5分間インキュベートさせた。IL-8プロモーターは、IL-33占有によるST2-IL-1RAcP受容体複合体の刺激により開始されるシグナル伝達カスケードに反応する。AB4111(2 μg)をFUGENETM混合物に添加し、室温で25分間インキュベートさせた。次いで、DNA/FUGENETM混合物をHEK/ST2細胞上に供給し、8時間インキュベートさせた。細胞をACCUTASETMで採取し、0.1 mL DMEM/10%FBS中、ウェルあたり2.0×104細胞で96ウェル平底プレートに播種した。プレートを37℃、5%CO2で15〜18時間インキュベートした。翌朝、プレートを穏やかに裏返し、ペーパータオル上でタップして培地を除去した。50 μL/ウェルの新鮮なDMEM/10%FBSを各ウェルに添加した。室温で20分間、予め複合体化されたIL-33/ST2-Fc又はIL-33/Ab(次いで細胞に添加される)で細胞を刺激し、37℃でさらに5時間インキュベートさせた。5時間後、Steady Glo-Luciferase Assay System(Promega, Madison, WI)を用いて、ルシフェラーゼ試薬を1:1 vol/volで各ウェルに添加することにより、ルシフェラーゼ活性を決定した。ウェルを混合し、150 μL/ウェルを黒色壁で透明底のプレートに移し、ENVISTIONTMPlate Reader(PerkinElmer, Waltham, MA)にて、Luminescenceプログラム(60秒ディレイ)を用いて読み取った。GraphPad Prism 5ソフトウェア(GraphPad, San Diego, CA)を用いて4パラメータカーブフィット(4 parameter curve fit)によりデータを解析した。
抗IL33抗体の結合キネティクス及び親和性をBIACORETMT200装置(GE Healthcare)でのSPRにより決定した。Series S CM5チップ上の4つのフローセルそれぞれに、およそ10,000 RUの抗ヒトIgG(Fc)を固定化した。抗体(およそ1 μg/mL)を流速10 μL/分で60秒間キャプチャーした。モノマーのIL-33をランニングバッファー(HBS-EP+, pH 7.6)中、各抗体のKDよりもおよそ100倍高い濃度から開始して希釈した。各IL-33濃度について、30 μL/分で180秒間、すべてのフローセルを通過させ、次いで1800秒間解離させた。表面は、60秒間、3 M MgCl2を用いて再生させた。結合(association)及び解離速度定数(kinetic constants)(kon及びkoff)並びに定常状態親和性(KD)は、BIACORE T200 Evaluation Softwareバージョン1.0を用いて、得られたセンサーグラムから算出した。
本実施例は、本発明のIL-33結合剤の機能活性を実証する実験を記述する。
本実施例は、本発明のIL-33結合剤のIL-33に対する親和性を実証する。
50 μg/mLのヒスチジンタグ化ヒトIL-33溶液を用いてコーティングされたアズラクトンコーティングビーズを用いて固相を調製した。結合実験は、1× PBS pH 7.4, 0.1%BSA中で実施した。最終濃度10 pM(又は20 pM)のAPE4909抗体を、最終濃度200 pM〜3.4 fM(又は400 pM〜6.7 fM)のIL-33と4℃、3(又は4)日間インキュベートした。5 mL(又は10 mL)の各混合物を、IL-33でコーティングされたビーズに、速度0.25 mL/分で1200秒間(又は2400秒間)適用した。ALEXAFLUORTM 647-(Life Technologies, Carlsbad, CA)標識ロバ抗ヒト抗体を用いて遊離抗体を検出した。データはすべて、標準的なKINEXATMソフトウェアを用いてフィットした。
最終濃度20 pM及び100 pMのAPE4909抗体を、最終濃度3 nM〜315 fMのcIL-33と4℃、24時間インキュベートした以外は、ヒトIL-33に関して上に記載するように実験を実施した。各混合物を、cIL-33でコーティングされたビーズに、速度0.25 mL/分で500秒間(20 pMについて)又は2120秒間(100 pMについて)適用した。ALEXAFLUORTM 647-(Life Technologies, Carlsbad, CA)標識ロバ抗ヒト抗体を用いて遊離抗体を検出した。N曲線解析(N-curve analysis)を用いて2つの曲線を合わせ、データはすべて、KINEXATMソフトウェアを用いてフィットした。検証するために、200 pMのAPE4909抗体濃度で、同様に調製した固相、バッファー及び検出試薬を用いて実験を繰り返した。APE4909抗体を、最終濃度15 nM〜250 fMのcIL-33と4℃、24時間インキュベートし、cIL-33でコーティングされたビーズに、速度0.25 mL/分で180秒間適用した。このデータを標準的なKINEXATMソフトウェアを用いてフィットした。
本実施例は、特定の本発明のIL-33結合剤が、ヒトIL-33との結合についてST2受容体と競合することを実証する。
本実施例は、本発明のIL-33結合剤が、ヒトIL-33により推進される末梢好酸球の増殖(expansion)を阻害することを実証する。
Claims (33)
- (a)配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つのアミノ酸配列、又は(b)配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つと少なくとも90%同一であるアミノ酸配列を含む、単離された免疫グロブリン重鎖ポリペプチド。
- 請求項1に記載の免疫グロブリン重鎖ポリペプチドと同じIL-33エピトープと結合する、単離された免疫グロブリン重鎖ポリペプチド。
- (a)配列番号3、配列番号4、配列番号51〜66、配列番号141〜175、配列番号189〜205及び配列番号218〜231のいずれか1つのアミノ酸配列、又は(b)配列番号3、配列番号4、配列番号51〜66、配列番号141〜175、配列番号189〜205及び配列番号218〜231のいずれか1つと少なくとも90%同一であるアミノ酸配列を含む、単離された免疫グロブリン軽鎖ポリペプチド。
- 請求項3に記載の免疫グロブリン軽鎖ポリペプチドと同じIL-33エピトープと結合する、単離された免疫グロブリン軽鎖ポリペプチド。
- 請求項1又は2に記載の単離された免疫グロブリン重鎖ポリペプチドをコードする、単離又は精製された核酸配列。
- 請求項3又は4に記載の単離された免疫グロブリン軽鎖ポリペプチドをコードする、単離又は精製された核酸配列。
- 請求項5又は6に記載の単離又は精製された核酸分子を含む、ベクター。
- (a)請求項1に記載の免疫グロブリン重鎖ポリペプチド及び/又は(b)請求項2に記載の免疫グロブリン軽鎖ポリペプチドを含む、単離されたインターロイキン-33(IL-33)結合剤。
- 抗体、抗体コンジュゲート、又はその抗原結合フラグメントである、請求項8に記載の単離されたIL-33結合剤。
- F(ab’)2、Fab’、Fab、Fv、scFv、dsFv、dAb、及び一本鎖結合ポリペプチドから選択される抗体フラグメントである、請求項8に記載の単離されたIL-33結合剤。
- 配列番号136の免疫グロブリン重鎖ポリペプチド及び配列番号171の免疫グロブリン軽鎖ポリペプチドを含む、請求項8に記載の単離されたIL-33結合剤。
- 配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つを含む免疫グロブリン重鎖可変領域の少なくとも1つの相補性決定領域(CDR)を含む、単離されたIL-33結合剤。
- 配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つを含む免疫グロブリン重鎖可変領域の1つのCDRを含む、請求項12に記載の単離されたIL-33結合剤。
- 配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つを含む免疫グロブリン重鎖可変領域の2つのCDRsを含む、請求項12に記載の単離されたIL-33結合剤。
- 配列番号1、配列番号2、配列番号5〜50、配列番号67〜140、配列番号176、配列番号177、配列番号178〜188及び配列番号206〜217のいずれか1つを含む免疫グロブリン重鎖可変領域の3つのCDRsを含む、請求項12に記載の単離されたIL-33結合剤。
- 配列番号3、配列番号4、配列番号51〜66、配列番号141〜175、配列番号189〜205及び配列番号218〜231のいずれか1つを含む免疫グロブリン軽鎖可変領域の少なくとも1つのCDRを含む、単離されたIL-33結合剤。
- 配列番号3、配列番号4、配列番号51〜66、配列番号141〜175、配列番号189〜205及び配列番号218〜231のいずれか1つを含む免疫グロブリン軽鎖可変領域の1つのCDRを含む、請求項16に記載の単離されたIL-33結合剤。
- 配列番号3、配列番号4、配列番号51〜66、配列番号141〜175、配列番号189〜205及び配列番号218〜231のいずれか1つを含む免疫グロブリン軽鎖可変領域の2つのCDRsを含む、請求項16に記載の単離されたIL-33結合剤。
- 配列番号3、配列番号4、配列番号51〜66、配列番号141〜175、配列番号189〜205及び配列番号218〜231のいずれか1つを含む免疫グロブリン軽鎖可変領域の3つのCDRsを含む、請求項16に記載の単離されたIL-33結合剤。
- 請求項8〜19のいずれか一項に記載のIL-33結合剤をコードする、単離又は精製された核酸配列。
- 請求項20に記載の単離又は精製された核酸配列を含む、ベクター。
- 請求項21に記載のベクターを含む、単離された細胞。
- (a)請求項8〜19のいずれか一項に記載の単離されたIL-33結合剤又は請求項21に記載のベクター、及び(b)医薬上許容される担体を含む、組成物。
- 哺乳動物において、IL-33の阻害に反応する障害を治療する方法であって、IL-33の阻害に反応する障害を有する哺乳動物に、請求項23に記載の組成物の有効量を投与することを含み、そうすることで、該哺乳動物において該障害が治療される、方法。
- 障害が炎症性障害である、請求項24に記載の方法。
- 炎症性障害が、アトピー性皮膚炎、アレルギー性喘息、食物アレルギー、又は線維症である、請求項25に記載の方法。
- 食物アレルギーがピーナッツアレルギーである、請求項26に記載の方法。
- 障害が自己免疫疾患である、請求項24に記載の方法。
- 自己免疫疾患がクローン病又は関節リウマチである、請求項28に記載の方法。
- 障害が癌である、請求項24に記載の方法。
- 癌が、上皮癌、慢性骨髄性白血病(CML)、乳癌、又は消化器癌である、請求項30に記載の方法。
- 哺乳動物におけるIL-33結合剤の半減期が、30分間から45日間の間である、請求項24〜31のいずれか一項に記載の方法。
- IL-33結合剤が、約1フェムトモーラー(fM)から約100マイクロモーラー(μM)の間のKDでIL-33と結合する、請求項24〜32のいずれか一項に記載の方法。
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CN106103480B (zh) | 2021-10-22 |
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KR102446386B1 (ko) | 2022-09-22 |
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RU2016132757A (ru) | 2018-02-16 |
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US10059764B2 (en) | 2018-08-28 |
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US10836820B2 (en) | 2020-11-17 |
MX2020001272A (es) | 2020-07-20 |
CN106103480A (zh) | 2016-11-09 |
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