JP2016536983A5 - - Google Patents

Download PDF

Info

Publication number
JP2016536983A5
JP2016536983A5 JP2016525617A JP2016525617A JP2016536983A5 JP 2016536983 A5 JP2016536983 A5 JP 2016536983A5 JP 2016525617 A JP2016525617 A JP 2016525617A JP 2016525617 A JP2016525617 A JP 2016525617A JP 2016536983 A5 JP2016536983 A5 JP 2016536983A5
Authority
JP
Japan
Prior art keywords
region
microfluidic
channel
con
enclosure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2016525617A
Other languages
English (en)
Japanese (ja)
Other versions
JP6557658B2 (ja
JP2016536983A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2014/061837 external-priority patent/WO2015061497A1/en
Publication of JP2016536983A publication Critical patent/JP2016536983A/ja
Publication of JP2016536983A5 publication Critical patent/JP2016536983A5/ja
Priority to JP2019129854A priority Critical patent/JP6944490B2/ja
Application granted granted Critical
Publication of JP6557658B2 publication Critical patent/JP6557658B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2016525617A 2013-10-22 2014-10-22 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法 Active JP6557658B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019129854A JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361996969P 2013-10-22 2013-10-22
US61/996,969 2013-10-22
US201462058658P 2014-10-01 2014-10-01
US62/058,658 2014-10-01
US14/520,568 2014-10-22
PCT/US2014/061837 WO2015061497A1 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US14/520,568 US10010882B2 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019129854A Division JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Publications (3)

Publication Number Publication Date
JP2016536983A JP2016536983A (ja) 2016-12-01
JP2016536983A5 true JP2016536983A5 (enExample) 2017-11-30
JP6557658B2 JP6557658B2 (ja) 2019-08-07

Family

ID=52993522

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2016525617A Active JP6557658B2 (ja) 2013-10-22 2014-10-22 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2019129854A Active JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2021147377A Active JP7333365B2 (ja) 2013-10-22 2021-09-10 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2023091448A Pending JP2023115034A (ja) 2013-10-22 2023-06-02 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2019129854A Active JP6944490B2 (ja) 2013-10-22 2019-07-12 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2021147377A Active JP7333365B2 (ja) 2013-10-22 2021-09-10 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法
JP2023091448A Pending JP2023115034A (ja) 2013-10-22 2023-06-02 隔離囲いを有するマイクロ流体デバイスおよびそれによる生物学的微小物体の試験方法

Country Status (9)

Country Link
US (4) US10010882B2 (enExample)
EP (3) EP3760703A1 (enExample)
JP (4) JP6557658B2 (enExample)
KR (4) KR102221894B1 (enExample)
AU (3) AU2014340089B2 (enExample)
CA (2) CA2927701C (enExample)
IL (3) IL297654A (enExample)
SG (2) SG10202006525PA (enExample)
WO (1) WO2015061497A1 (enExample)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8535241B2 (en) 2011-10-13 2013-09-17 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
CN104508492B (zh) 2012-05-25 2018-07-27 北卡罗来纳-查佩尔山大学 微流体装置、用于试剂的固体支持体和相关方法
WO2014022275A1 (en) 2012-08-01 2014-02-06 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
CN104981203B (zh) 2012-12-04 2017-12-08 木兰医药技术股份有限公司 无菌体液采集装置和方法
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
CA2927701C (en) 2013-10-22 2020-10-13 Berkeley Lights, Inc. Microfluidic devices having sequestration pens and methods of testing biological micro-objects with same
GB201320146D0 (en) 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US20150352547A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating Microfluidic Structures and Trapping Bubbles
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
WO2016090295A1 (en) 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground
CN107249742B (zh) 2014-12-08 2019-12-06 伯克利之光生命科技公司 微流体装置中定向流动的致动微流体结构及使用它的方法
JP6514776B2 (ja) 2014-12-08 2019-05-15 バークレー ライツ,インコーポレイテッド 横方向/垂直方向トランジスタ構造を含むマイクロ流体デバイスならびにそれを作製および使用するプロセス
CN113075113A (zh) 2014-12-09 2021-07-06 伯克利之光生命科技公司 微流体装置中微物体的自动检测和重新定位
EP3230718B1 (en) 2014-12-09 2022-03-02 Berkeley Lights, Inc. Automated detection of assay-positive areas or of analyte quantities in microfluidic devices
US9744533B2 (en) * 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
CA2970149C (en) 2014-12-10 2022-12-13 Berkeley Lights, Inc. Systems for operating electrokinetic devices
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
US10973227B2 (en) 2015-04-22 2021-04-13 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
TWI712686B (zh) * 2015-04-22 2020-12-11 美商柏克萊燈光有限公司 用於微流體裝置之培養站
AU2016250689B2 (en) 2015-04-22 2021-07-08 Berkeley Lights, Inc. Microfluidic cell culture
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
CN114272966B (zh) * 2015-07-22 2023-11-07 北卡罗来纳-查佩尔山大学 含有具有空间分离的珠保留和信号检测段的珠孔几何结构的流体装置和相关方法
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
AU2016361413B2 (en) * 2015-11-23 2022-04-14 Berkeley Lights, Inc. In situ-generated microfluidic isolation structures, kits and methods of use thereof
CN108603878A (zh) * 2015-12-08 2018-09-28 伯克利之光生命科技公司 微流体设备和试剂盒及其使用方法
KR20230042540A (ko) 2015-12-30 2023-03-28 버클리 라잇츠, 인크. 광학적으로 구동되는 대류 및 변위를 위한 미세유체 디바이스들, 키트들 및 그 방법들
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
US11420204B2 (en) 2016-01-13 2022-08-23 Duke University Platforms for single cell analysis
US10712344B2 (en) * 2016-01-15 2020-07-14 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
EP3429753A4 (en) 2016-03-16 2019-11-06 Berkeley Lights, Inc. METHODS, SYSTEMS AND DEVICES FOR SELECTION AND PRODUCTION OF GENERICALLY WORKED CLONES
KR102423945B1 (ko) * 2016-03-17 2022-07-22 버클리 라잇츠, 인크. 미세유체 디바이스에서의 t 림프구들의 선택 및 클로닝
EP4043475B1 (en) * 2016-03-31 2023-09-20 Berkeley Lights, Inc. Nucleic acid stabilization reagent, kits, and methods of use thereof
AU2017248822B2 (en) * 2016-04-15 2022-09-15 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
AU2017271673B2 (en) 2016-05-26 2022-04-14 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use
AU2017298545B2 (en) * 2016-07-21 2022-10-27 Berkeley Lights, Inc. Sorting of T lymphocytes in a microfluidic device
WO2018064640A1 (en) * 2016-10-01 2018-04-05 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
CA3041527C (en) 2016-10-23 2023-09-05 Berkeley Lights, Inc. Methods for screening b cell lymphocytes
KR102551430B1 (ko) 2016-12-01 2023-07-05 버클리 라잇츠, 인크. 미세-객체들을 이미징하기 위한 장치들, 시스템들 및 방법들
TWI795380B (zh) 2016-12-01 2023-03-11 美商伯克利之光生命科技公司 針對微流體裝置中的微物件之自動偵測及重定位
JP7356351B2 (ja) 2016-12-12 2023-10-04 エクセラ・バイオサイエンシーズ・インコーポレイテッド マイクロキャピラリーアレイを使用したスクリーニングのための方法およびシステム
WO2018125832A1 (en) 2016-12-30 2018-07-05 xCella Biosciences, Inc. Multi-stage sample recovery system
CA3048645A1 (en) * 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
WO2018208332A2 (en) 2017-02-17 2018-11-15 Koniku, Inc. Systems for detection
JP7227162B2 (ja) 2017-06-06 2023-02-21 ザイマージェン インコーポレイテッド 真菌株を改良するためのhtpゲノム操作プラットフォーム
JP7273807B2 (ja) 2017-06-09 2023-05-15 マグノリア メディカル テクノロジーズ,インコーポレイテッド 流体制御デバイス及びそれを使用する方法
JP2020528747A (ja) 2017-07-21 2020-10-01 バークレー ライツ,インコーポレイテッド 抗原提示合成表面、共有結合官能化表面、活性化t細胞及びそれらの使用
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
CN112154190A (zh) 2018-05-18 2020-12-29 北卡罗来纳-查佩尔山大学 用于改善基材的表面性质的组合物、装置和方法
SG11202011180UA (en) * 2018-05-31 2020-12-30 Berkeley Lights Inc Automated detection and characterization of micro-objects in microfluidic devices
JP2021526799A (ja) 2018-06-06 2021-10-11 ザイマージェン インコーポレイテッド 発酵および産生中の真菌の形態を制御するためのシグナル伝達に関与する遺伝子の操作
EP3849705A4 (en) * 2018-09-14 2022-06-01 Berkeley Lights, Inc. Methods for assaying binding affinity
WO2020061499A1 (en) 2018-09-21 2020-03-26 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
AU2019358198A1 (en) 2018-10-11 2021-05-27 Berkeley Lights, Inc. Systems and methods for identification of optimized protein production and kits therefor
US20200139362A1 (en) 2018-10-18 2020-05-07 Berkeley Lights, Inc. Proto-Antigen-Presenting Synthetic Surfaces, Activated T Cells, and Uses Thereof
KR20210089193A (ko) 2018-11-01 2021-07-15 버클리 라잇츠, 인크. 미세유체 디바이스에서 생물학적 세포를 분석하기 위한 방법
SG11202104544WA (en) 2018-11-19 2021-06-29 Berkeley Lights Inc Microfluidic device with programmable switching elements
ES3039910T3 (en) 2018-12-06 2025-10-27 Xcella Biosciences Inc Lateral loading of microcapillary arrays
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
IT201900002777A1 (it) * 2019-02-26 2020-08-26 Menarini Silicon Biosystems Spa Metodo e sistema microfluidico per l'isolamento di particelle
CN114126762B (zh) 2019-04-30 2023-01-03 伯克利之光生命科技公司 用于包封和测定细胞的方法
TW202142856A (zh) 2019-11-17 2021-11-16 美商伯克利之光生命科技公司 用於生物樣本之分析的系統及方法
CA3170874A1 (en) 2020-03-09 2021-09-16 Phillip J. M. ELMS Methods, systems and kits for in-pen assays
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
TW202223389A (zh) 2020-09-07 2022-06-16 美商伯克利之光生命科技公司 檢測生物細胞之方法

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2288760T3 (es) 1996-04-25 2008-01-16 Bioarray Solutions Ltd. Ensamblaje electrocinetico controlado por luz de particulas proximas a superficies.
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1129772A3 (en) * 1999-03-03 2001-11-07 Symyx Technologies, Inc. Fluid distribution for chemical processing microsystems
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
EP1065378B1 (en) 1999-06-28 2002-04-03 California Institute of Technology Microfabricated elastomeric valve and pump systems
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
AU2004228678A1 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
WO2005072399A2 (en) 2004-01-29 2005-08-11 Massachusetts Institute Of Technology Microscale sorting cytometer
US8765454B2 (en) 2004-02-18 2014-07-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
WO2005121767A1 (ja) * 2004-05-25 2005-12-22 Fluid Incorporated マイクロ流体デバイス及びこれを用いる分析分取装置
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
CN101548004A (zh) 2005-08-19 2009-09-30 加利福尼亚大学董事会 用于诊断学和细胞分析的微流体方法
CA2616504A1 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Microfluidic methods for diagnostics and cellular analysis
US20070095667A1 (en) 2005-10-27 2007-05-03 Applera Corporation Optoelectronic Separation of Biomolecules
US7964078B2 (en) 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
ES2692380T3 (es) 2006-03-24 2018-12-03 Handylab, Inc. Método para realizar PCR con un cartucho con varias pistas
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
EP2041573B1 (en) 2006-06-23 2019-09-04 PerkinElmer Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
ATE554859T1 (de) 2007-05-24 2012-05-15 Univ California Integrierte fluidische vorrichtungen mit magnetischer sortierung
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
EP2212437A4 (en) 2007-11-07 2011-09-28 Univ British Columbia MICROFLUIDIC DEVICE AND METHOD OF USING THE DEVICE
CN103353476B (zh) 2008-04-03 2016-05-25 加利福尼亚大学董事会 分离并离析细胞、囊泡、纳米颗粒和生物标记的离体多维系统
US20110117634A1 (en) 2008-04-21 2011-05-19 Asaf Halamish Flat cell carriers with cell traps
CN101275114A (zh) 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
KR100991752B1 (ko) 2008-07-15 2010-11-03 한국과학기술원 단일 평면 광전자 소자를 이용한 미세입자 구동장치 및구동방법
WO2010014078A1 (en) 2008-07-28 2010-02-04 Agere Systems Inc. Systems and methods for variable compensated fly height measurement
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
CN102449163A (zh) 2009-04-03 2012-05-09 加利福尼亚大学董事会 分选细胞和其它生物微粒的方法和装置
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (ja) 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
US20110003330A1 (en) 2009-07-06 2011-01-06 Durack Gary P Microfluidic device
CN102472697A (zh) 2009-07-06 2012-05-23 索尼公司 微流体装置
US20110143378A1 (en) 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
US20130118905A1 (en) 2010-05-26 2013-05-16 Tosoh Corporation Biological sample immobilizing apparatus
CN101947124B (zh) 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
US9133499B2 (en) 2010-09-14 2015-09-15 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
US10571475B2 (en) * 2010-12-03 2020-02-25 Cellply S.R.L. Rapid screening of monoclonal antibodies
CN103392124B (zh) * 2010-12-03 2016-04-20 塞普莱有限公司 细胞功能的微分析
CN116179323A (zh) 2011-05-27 2023-05-30 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
US10626435B2 (en) * 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
TWI512383B (zh) 2012-07-04 2015-12-11 Ind Tech Res Inst 光學感應式介電泳裝置
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
CN109342710A (zh) 2013-03-28 2019-02-15 英属哥伦比亚大学 微流控装置及其在多细胞分泌检测中的使用方法
EP3783094B1 (en) 2013-10-22 2023-10-11 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
CA2927701C (en) 2013-10-22 2020-10-13 Berkeley Lights, Inc. Microfluidic devices having sequestration pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
JP6514776B2 (ja) 2014-12-08 2019-05-15 バークレー ライツ,インコーポレイテッド 横方向/垂直方向トランジスタ構造を含むマイクロ流体デバイスならびにそれを作製および使用するプロセス
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
AU2016250689B2 (en) 2015-04-22 2021-07-08 Berkeley Lights, Inc. Microfluidic cell culture

Similar Documents

Publication Publication Date Title
JP2016536983A5 (enExample)
JP5964881B2 (ja) プリント回路基板上の液滴操作装置及び方法
US10376886B2 (en) Micro-fluidic devices for assaying biological activity
EP3783094B1 (en) Micro-fluidic devices for assaying biological activity
Voldman et al. A microfabrication-based dynamic array cytometer
EP3060645B1 (en) Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
Chai et al. Capillarity enabled large‐array liquid metal electrodes for compact and high‐throughput dielectrophoretic microfluidics
US8551333B2 (en) Particle-based microfluidic device for providing high magnetic field gradients
US11911763B2 (en) Microfluidic device and methods
CN111957453B (zh) 使用作为流体输入的接通致动器储液器来提高液滴计量的准确度和精度的方法
US20170043343A1 (en) Dep force control and electrowetting control in different sections of the same microfluidic apparatus
Hyun et al. Microfluidic devices for the isolation of circulating rare cells: A focus on affinity‐based, dielectrophoresis, and hydrophoresis
Tong et al. Combining sensors and actuators with electrowetting-on-dielectric (EWOD): Advanced digital microfluidic systems for biomedical applications
CN106255888A (zh) 在同一微流体装置的不同部分中的dep力控制和电润湿控制
US20170205330A1 (en) Particle screening device
EP3473699B1 (en) Exporting a selected group of micro-objects from a micro-fluidic device
US10308977B2 (en) Device and method for processing a biological sample and analysis system for analyzing a biological specimen
US20140348706A1 (en) Microfluidic Device and Microfluidic System
Vaidyanathan et al. Microfluidics for cell sorting and single cell analysis from whole blood
EP2798055A2 (en) Two dimensional nanofluidic ccd arrays for manipulation of charged molecules in solution
CN104789443B (zh) 一种细胞定位单元、阵列、器件及其形成方法
JP2005292115A (ja) 高速スクリーニングまたは高速分析のための微小流体チップ
Sabaté del Río et al. Microfluidic Dielectrophoretic Purification of Extracellular Vesicles from Plasma Lipoproteins
US20240189821A1 (en) Method and Device for Trapping at Least One Nucleated Cell Using at Least One Electrode for a Microfluidic Device
US11446666B2 (en) Fluid handling device