SG10202006525PA - Microfluidic devices having isolation pens and methods of testing biological micro-objects with same - Google Patents

Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Info

Publication number
SG10202006525PA
SG10202006525PA SG10202006525PA SG10202006525PA SG10202006525PA SG 10202006525P A SG10202006525P A SG 10202006525PA SG 10202006525P A SG10202006525P A SG 10202006525PA SG 10202006525P A SG10202006525P A SG 10202006525PA SG 10202006525P A SG10202006525P A SG 10202006525PA
Authority
SG
Singapore
Prior art keywords
objects
methods
same
microfluidic devices
biological micro
Prior art date
Application number
SG10202006525PA
Other languages
English (en)
Inventor
Eric D Hobbs
Mark P White
J Tanner Nevill
Daniele Malleo
Steven W Short
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52993522&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10202006525P(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG10202006525PA publication Critical patent/SG10202006525PA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG10202006525PA 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same SG10202006525PA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361996969P 2013-10-22 2013-10-22
US201462058658P 2014-10-01 2014-10-01
US14/520,568 US10010882B2 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Publications (1)

Publication Number Publication Date
SG10202006525PA true SG10202006525PA (en) 2020-08-28

Family

ID=52993522

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201602629RA SG11201602629RA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
SG10202006525PA SG10202006525PA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201602629RA SG11201602629RA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Country Status (9)

Country Link
US (4) US10010882B2 (enExample)
EP (3) EP3473700B1 (enExample)
JP (4) JP6557658B2 (enExample)
KR (4) KR102113377B1 (enExample)
AU (3) AU2014340089B2 (enExample)
CA (2) CA2927701C (enExample)
IL (3) IL297654A (enExample)
SG (2) SG11201602629RA (enExample)
WO (1) WO2015061497A1 (enExample)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8535241B2 (en) 2011-10-13 2013-09-17 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
US9617589B2 (en) 2012-05-25 2017-04-11 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
WO2014022275A1 (en) 2012-08-01 2014-02-06 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
CN107874786B (zh) 2012-12-04 2020-11-24 木兰医药技术股份有限公司 用于从患者获取体液样本的设备
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
SG11201602629RA (en) * 2013-10-22 2016-05-30 Berkeley Lights Inc Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
GB201320146D0 (en) 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US20150352547A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating Microfluidic Structures and Trapping Bubbles
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
TWI698282B (zh) 2014-12-05 2020-07-11 加州大學董事會 基板、微流體元件、移動微流體元件中的液滴之方法以及用於操控微流體元件中的液滴之程序
EP3831482B1 (en) 2014-12-08 2024-01-24 Berkeley Lights, Inc. Microfluidic device comprising lateral/vertical transistor structures
EP3610946A1 (en) 2014-12-08 2020-02-19 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof cross reference to related application(s)
CN107206377B (zh) 2014-12-09 2020-02-11 伯克利之光生命科技公司 微流体装置中测定阳性的区域的自动检测
CN113075113A (zh) 2014-12-09 2021-07-06 伯克利之光生命科技公司 微流体装置中微物体的自动检测和重新定位
US9744533B2 (en) * 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
SG11201704659PA (en) 2014-12-10 2017-07-28 Berkeley Lights Inc Systems for operating electrokinetic devices
SG11201708429WA (en) * 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture
HK1248750A1 (zh) * 2015-04-22 2018-10-19 伯克利之光生命科技公司 用於微流体装置的培养站
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
JP2018512875A (ja) 2015-04-22 2018-05-24 バークレー ライツ，インコーポレイテッド マイクロ流体デバイスにおける細胞の凍結及び保管
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
WO2017065854A2 (en) * 2015-07-22 2017-04-20 The University Of North Carolina At Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
US11666913B2 (en) * 2015-11-23 2023-06-06 Berkeley Lights, Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
TWI847100B (zh) * 2015-12-08 2024-07-01 美商布魯克細胞分析技術公司 在微流體器件中實施原位捕獲分析之套組
SG11201805473SA (en) * 2015-12-30 2018-07-30 Berkeley Lights Inc Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
WO2017123697A1 (en) * 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis
WO2017123978A1 (en) 2016-01-15 2017-07-20 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
EP3429753A4 (en) 2016-03-16 2019-11-06 Berkeley Lights, Inc. METHODS, SYSTEMS AND DEVICES FOR SELECTION AND PRODUCTION OF GENERICALLY WORKED CLONES
EP3430131B1 (en) * 2016-03-17 2022-06-08 Berkeley Lights, Inc. Selection and cloning of t lymphocytes in a microfluidic device
WO2017173105A1 (en) 2016-03-31 2017-10-05 Berkeley Lights, Inc. Nucleic acid stabilization reagent, kits, and methods of use thereof
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
CA3020976A1 (en) * 2016-04-15 2017-10-19 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
SG10202008265XA (en) 2016-05-26 2020-09-29 Berkeley Lights Inc Covalently modified surfaces, kits, and methods of preparation and use
SG11201900442PA (en) * 2016-07-21 2019-02-27 Berkeley Lights Inc Sorting of t lymphocytes in a microfluidic device
EP3519612B1 (en) 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
WO2018076024A2 (en) * 2016-10-23 2018-04-26 Berkeley Lights, Inc. Methods for screening b cell lymphocytes
JP2019537067A (ja) 2016-12-01 2019-12-19 バークレー ライツ，インコーポレイテッド 微小物体を撮像する装置、システム、及び方法
IL267009B (en) 2016-12-01 2022-07-01 Berkeley Lights Inc Automated detection and repositioning of micro-objects in microfluidic devices
AU2017375631B2 (en) 2016-12-12 2023-06-15 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
WO2018125832A1 (en) 2016-12-30 2018-07-05 xCella Biosciences, Inc. Multi-stage sample recovery system
WO2018126205A1 (en) * 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
US11474103B2 (en) 2017-02-17 2022-10-18 Koniku Inc. Systems for detection
WO2018226900A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
JP7273807B2 (ja) 2017-06-09 2023-05-15 マグノリア メディカル テクノロジーズ，インコーポレイテッド 流体制御デバイス及びそれを使用する方法
CN118846017A (zh) 2017-07-21 2024-10-29 伯克利之光生命科技公司 抗原呈递合成表面、共价官能化表面、活化t细胞及其用途
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
US12234377B2 (en) 2018-05-18 2025-02-25 The University Of North Carolina At Chapel Hill Compositions, devices, and methods for improving a surface property of a substrate
CN112204380B (zh) * 2018-05-31 2024-11-05 伯克利之光生命科技公司 微流体设备中的微物体的自动检测和表征
WO2019236848A1 (en) 2018-06-06 2019-12-12 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
CA3112333A1 (en) * 2018-09-14 2020-03-19 Berkeley Lights, Inc. Methods for assaying binding affinity
AU2019343994A1 (en) 2018-09-21 2021-05-13 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
EP3863757A4 (en) 2018-10-11 2022-07-20 Berkeley Lights, Inc. SYSTEMS AND METHODS FOR IDENTIFICATION OF OPTIMIZED PROTEIN PRODUCTION AND KITS THEREFORE
US20200139362A1 (en) 2018-10-18 2020-05-07 Berkeley Lights, Inc. Proto-Antigen-Presenting Synthetic Surfaces, Activated T Cells, and Uses Thereof
CA3118957A1 (en) * 2018-11-01 2020-05-07 Berkeley Lights, Inc. Methods for assaying biological cells in a microfluidic environment
SG11202104544WA (en) 2018-11-19 2021-06-29 Berkeley Lights Inc Microfluidic device with programmable switching elements
DK3890876T3 (da) 2018-12-06 2024-07-22 Xcella Biosciences Inc Lateral påfyldning af mikrokapillære arrays
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
IT201900002777A1 (it) * 2019-02-26 2020-08-26 Menarini Silicon Biosystems Spa Metodo e sistema microfluidico per l'isolamento di particelle
EP3962652A4 (en) 2019-04-30 2023-01-18 Berkeley Lights, Inc. CELL ENCAPSULATION AND ANALYSIS PROCESSES
TW202142856A (zh) 2019-11-17 2021-11-16 美商伯克利之光生命科技公司 用於生物樣本之分析的系統及方法
IL296312A (en) 2020-03-09 2022-11-01 Berkeley Lights Inc Methods, systems and kits for tests to be performed inside a pen
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
EP4204810A4 (en) 2020-09-07 2024-10-09 Berkeley Lights, Inc. METHODS FOR DOSING A BIOLOGICAL CELL

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2255599C (en) 1996-04-25 2006-09-05 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1106244A3 (en) * 1999-03-03 2001-11-21 Symyx Technologies, Inc. Chemical processing microsystems and controlling reaction conditions in same
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
MXPA01012959A (es) 1999-06-28 2002-07-30 California Inst Of Techn Sistemas elastomericos, microfabricados, de valvulas y bombas.
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
EP1499706A4 (en) 2002-04-01 2010-11-03 Fluidigm Corp MICROFLUIDIC PARTICLE ANALYSIS SYSTEMS
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
EP1615721B1 (en) 2003-04-03 2014-06-18 Fluidigm Corporation Microfluidic devices and methods of using same
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
WO2005072399A2 (en) 2004-01-29 2005-08-11 Massachusetts Institute Of Technology Microscale sorting cytometer
CN1942590B (zh) 2004-02-18 2012-09-05 周小川 多元化学和生化反应的流体装置和方法
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
US20070240495A1 (en) * 2004-05-25 2007-10-18 Shuzo Hirahara Microfluidic Device and Analyzing/Sorting Apparatus Using The Same
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
EP1904619A4 (en) 2005-07-07 2012-05-02 Univ California METHOD AND DEVICE FOR CELL CULTURE ARRAY
US20100003666A1 (en) 2005-08-19 2010-01-07 The Regents Of The University Of California Microfluidic Methods for Diagnostics and Cellular Analysis
CN101548004A (zh) 2005-08-19 2009-09-30 加利福尼亚大学董事会 用于诊断学和细胞分析的微流体方法
US20070095667A1 (en) 2005-10-27 2007-05-03 Applera Corporation Optoelectronic Separation of Biomolecules
US7964078B2 (en) * 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
WO2008002462A2 (en) 2006-06-23 2008-01-03 Micronics, Inc. Methods and devices for microfluidic point-of-care immunoassays
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
US9040288B2 (en) 2006-03-24 2015-05-26 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
WO2008147530A1 (en) 2007-05-24 2008-12-04 The Regents Of The University Of California Integrated fluidics devices with magnetic sorting
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
JP5404638B2 (ja) 2007-11-07 2014-02-05 ザ ユニヴァーシティ オブ ブリティッシュ コロンビア マイクロ流体デバイスおよびその使用方法
US20110108422A1 (en) 2008-04-03 2011-05-12 The Regents Of The University Of California Ex vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
WO2009130694A2 (en) * 2008-04-21 2009-10-29 Cell Kinetics Ltd. Flat cell carriers with cell traps
CN101275114A (zh) 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
KR100991752B1 (ko) 2008-07-15 2010-11-03 한국과학기술원 단일 평면 광전자 소자를 이용한 미세입자 구동장치 및구동방법
WO2010014078A1 (en) 2008-07-28 2010-02-04 Agere Systems Inc. Systems and methods for variable compensated fly height measurement
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
CN102449163A (zh) 2009-04-03 2012-05-09 加利福尼亚大学董事会 分选细胞和其它生物微粒的方法和装置
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (ja) 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
CN102472697A (zh) 2009-07-06 2012-05-23 索尼公司 微流体装置
US20110003330A1 (en) 2009-07-06 2011-01-06 Durack Gary P Microfluidic device
US20110143378A1 (en) 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
JPWO2011149032A1 (ja) 2010-05-26 2013-07-25 東ソー株式会社 生体試料固定装置
CN101947124B (zh) * 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
CA2809877C (en) 2010-09-14 2021-11-23 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
EP2646830B1 (en) * 2010-12-03 2016-04-13 Cellply S.R.L. Rapid screening of monoclonal antibodies
EP2646798B1 (en) * 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
CA2836608C (en) 2011-05-27 2016-10-11 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
WO2013148745A1 (en) * 2012-03-28 2013-10-03 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
US9144806B2 (en) 2012-07-04 2015-09-29 Industrial Technology Research Institute Optically-induced dielectrophoresis device
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
CA2906231C (en) 2013-03-28 2024-05-14 Marketa RICICOVA Microfluidic devices and methods for use thereof in multicellular assays of secretion
SG11201602629RA (en) 2013-10-22 2016-05-30 Berkeley Lights Inc Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
AU2014340098B2 (en) 2013-10-22 2020-05-28 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3831482B1 (en) 2014-12-08 2024-01-24 Berkeley Lights, Inc. Microfluidic device comprising lateral/vertical transistor structures
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
SG11201708429WA (en) 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture

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