IL297654A - Microfluidic devices having isolation pens and methods of testing biological micro-objects with same - Google Patents

Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Info

Publication number
IL297654A
IL297654A IL297654A IL29765422A IL297654A IL 297654 A IL297654 A IL 297654A IL 297654 A IL297654 A IL 297654A IL 29765422 A IL29765422 A IL 29765422A IL 297654 A IL297654 A IL 297654A
Authority
IL
Israel
Prior art keywords
channel
objects
microfluidic
micro
microns
Prior art date
Application number
IL297654A
Other languages
English (en)
Hebrew (he)
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52993522&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL297654(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of IL297654A publication Critical patent/IL297654A/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL297654A 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same IL297654A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361996969P 2013-10-22 2013-10-22
US201462058658P 2014-10-01 2014-10-01
PCT/US2014/061837 WO2015061497A1 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US14/520,568 US10010882B2 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Publications (1)

Publication Number Publication Date
IL297654A true IL297654A (en) 2022-12-01

Family

ID=52993522

Family Applications (3)

Application Number Title Priority Date Filing Date
IL297654A IL297654A (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
IL283153A IL283153B2 (en) 2013-10-22 2014-10-22 Microfluidic devices with isolation pens and methods for testing microbiological items using them
IL245254A IL245254B (en) 2013-10-22 2016-04-21 Microfluidic devices with isolation pens and methods for testing microbiological items using them

Family Applications After (2)

Application Number Title Priority Date Filing Date
IL283153A IL283153B2 (en) 2013-10-22 2014-10-22 Microfluidic devices with isolation pens and methods for testing microbiological items using them
IL245254A IL245254B (en) 2013-10-22 2016-04-21 Microfluidic devices with isolation pens and methods for testing microbiological items using them

Country Status (9)

Country Link
US (4) US10010882B2 (enExample)
EP (3) EP3060645B1 (enExample)
JP (4) JP6557658B2 (enExample)
KR (4) KR102221894B1 (enExample)
AU (3) AU2014340089B2 (enExample)
CA (2) CA3095333C (enExample)
IL (3) IL297654A (enExample)
SG (2) SG11201602629RA (enExample)
WO (1) WO2015061497A1 (enExample)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8535241B2 (en) 2011-10-13 2013-09-17 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
JP6200948B2 (ja) 2012-05-25 2017-09-20 ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル マイクロ流体デバイス
US9022951B2 (en) 2012-05-30 2015-05-05 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
US9060724B2 (en) 2012-05-30 2015-06-23 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
US9204864B2 (en) 2012-08-01 2015-12-08 Magnolia Medical Technologies, Inc. Fluid diversion mechanism for bodily-fluid sampling
ES2878047T3 (es) 2012-10-11 2021-11-18 Magnolia Medical Technologies Inc Sistema para administrar un fluido a un paciente con contaminación reducida
CA3136331C (en) 2012-11-30 2024-05-21 Magnolia Medical Technologies, Inc. Syringe based fluid diversion mechanism for bodily-fluid sampling
EP3305196B8 (en) 2012-12-04 2023-03-08 Magnolia Medical Technologies, Inc. Sterile bodily-fluid collection device
KR102221894B1 (ko) * 2013-10-22 2021-03-03 버클리 라잇츠, 인크. 격리 펜을 갖는 미세유체 소자 및 이를 사용한 생물학적 미세 물체를 시험하는 방법
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
GB201320146D0 (en) 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
WO2015188171A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating microfluidic structures and trapping bubbles
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
US9815056B2 (en) 2014-12-05 2017-11-14 The Regents Of The University Of California Single sided light-actuated microfluidic device with integrated mesh ground
US10058865B2 (en) 2014-12-08 2018-08-28 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof
KR102369770B1 (ko) 2014-12-08 2022-03-02 버클리 라잇츠, 인크. 측방향/수직 트랜지스터 구조들을 포함하는 미세유체 디바이스 및 그 제조 및 사용 프로세스
WO2016094459A2 (en) 2014-12-09 2016-06-16 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
CN107206377B (zh) 2014-12-09 2020-02-11 伯克利之光生命科技公司 微流体装置中测定阳性的区域的自动检测
WO2016094715A2 (en) * 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
JP2018508743A (ja) 2014-12-10 2018-03-29 バークレー ライツ,インコーポレイテッド 界面動電装置を作動させるためのシステム
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
CA2982252A1 (en) * 2015-04-22 2016-10-27 Berkeley Lights, Inc. Microfluidic device for culturing biological cells and methods of use therof
AU2016250580B2 (en) * 2015-04-22 2021-02-25 Berkeley Lights, Inc. Culturing station for microfluidic device
AU2016252995B2 (en) 2015-04-22 2020-12-03 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
US10870111B2 (en) 2015-07-22 2020-12-22 The University Of North Carolina At Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
KR20180085783A (ko) 2015-11-23 2018-07-27 버클리 라잇츠, 인크. 인 시츄-생성된 미세유체 격리 구조체들, 키트들, 및 그 이용 방법들
IL259837B2 (en) * 2015-12-08 2023-03-01 Berkeley Lights Inc Microfluidic devices and kits and their uses
KR20230042540A (ko) * 2015-12-30 2023-03-28 버클리 라잇츠, 인크. 광학적으로 구동되는 대류 및 변위를 위한 미세유체 디바이스들, 키트들 및 그 방법들
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
WO2017123697A1 (en) * 2016-01-13 2017-07-20 Duke University Platforms for single cell analysis
EP3402814B1 (en) * 2016-01-15 2021-04-21 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
DK3430131T3 (da) * 2016-03-17 2022-08-22 Berkeley Lights Inc Selektion og kloning af t-lymfocytter i en mikrofluidanordning
KR102451535B1 (ko) 2016-03-31 2022-10-05 버클리 라잇츠, 인크. 핵산 안정화 시약, 키트들, 및 그 이용 방법들
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
JP6985289B2 (ja) 2016-04-15 2021-12-22 バークレー ライツ,インコーポレイテッド ペン内アッセイの方法、システム及びキット
KR102691104B1 (ko) 2016-05-26 2024-08-01 버클리 라잇츠, 인크. 공유 결합으로 개질된 표면들, 키트들, 및 준비 및 사용 방법들
SG11201900442PA (en) * 2016-07-21 2019-02-27 Berkeley Lights Inc Sorting of t lymphocytes in a microfluidic device
AU2017336164A1 (en) 2016-10-01 2019-04-18 Berkeley Lights, Inc. DNA barcode compositions and methods of in situ identification in a microfluidic device
AU2017345813B2 (en) * 2016-10-23 2024-06-13 Berkeley Lights, Inc. Methods for screening B cell lymphocytes
CA3045333A1 (en) 2016-12-01 2018-06-07 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
CN114414510A (zh) 2016-12-01 2022-04-29 伯克利之光生命科技公司 用于对微物体成像的设备、系统和方法
EP3551745A4 (en) 2016-12-12 2020-07-15 Xcella Biosciences, Inc. SCREENING METHODS AND SYSTEMS USING MICROCAPILLARY NETWORKS
CN110382439B (zh) 2016-12-30 2026-07-21 埃克切拉生物科学公司 多级样品回收系统
CN110546495B (zh) * 2016-12-30 2022-11-01 加利福尼亚州立大学董事会 用于基因组编辑t细胞的选择和传代的方法
JP2020510417A (ja) 2017-02-17 2020-04-09 コニク インコーポレイテッド 検出のためのシステム
JP7227162B2 (ja) 2017-06-06 2023-02-21 ザイマージェン インコーポレイテッド 真菌株を改良するためのhtpゲノム操作プラットフォーム
IL271054B2 (en) 2017-06-09 2024-05-01 Magnolia Medical Technologies Inc Fluid control devices and methods of using the same
KR20200030593A (ko) 2017-07-21 2020-03-20 버클리 라잇츠, 인크. 항원 제시 합성 표면, 공유결합으로 관능화된 표면, 활성화된 t 세포, 및 이들의 용도
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
JP7556790B2 (ja) 2018-05-18 2024-09-26 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル 基板の表面特性を改善する為の組成物、デバイス、及び方法
CA3100701A1 (en) 2018-05-31 2019-12-05 Berkeley Lights, Inc. Automated detection and characterization of micro-objects in microfluidic devices
WO2019236848A1 (en) 2018-06-06 2019-12-12 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
EP3849705A4 (en) * 2018-09-14 2022-06-01 Berkeley Lights, Inc. Methods for assaying binding affinity
KR20210063362A (ko) 2018-09-21 2021-06-01 버클리 라잇츠, 인크. 작용화된 웰 플레이트, 이의 제조 및 사용 방법
CN113195085B (zh) 2018-10-11 2024-04-19 伯克利之光生命科技公司 用于识别优化蛋白产生的系统和方法以及其试剂盒
WO2020081875A1 (en) 2018-10-18 2020-04-23 Berkeley Lights, Inc. Proto-antigen-presenting synthetic surfaces, activated t cells, and uses thereof
EP3874265A4 (en) 2018-11-01 2022-09-28 Berkeley Lights, Inc. Methods for assaying biological cells in a microfluidic device
WO2020106646A1 (en) 2018-11-19 2020-05-28 Berkeley Lights, Inc. Microfluidic device with programmable switching elements
DK3890876T3 (da) 2018-12-06 2024-07-22 Xcella Biosciences Inc Lateral påfyldning af mikrokapillære arrays
JP7553455B2 (ja) 2019-02-08 2024-09-18 マグノリア メディカル テクノロジーズ,インコーポレイテッド 体液採取及び分配のための装置及び方法
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
IT201900002777A1 (it) * 2019-02-26 2020-08-26 Menarini Silicon Biosystems Spa Metodo e sistema microfluidico per l'isolamento di particelle
EP3962652A4 (en) 2019-04-30 2023-01-18 Berkeley Lights, Inc. CELL ENCAPSULATION AND ANALYSIS PROCESSES
CA3156776A1 (en) 2019-11-08 2021-05-14 xCella Biosciences, Inc. Apparatus and methods for laser-based single cell recovery from microcapillary arrays
CA3157616A1 (en) 2019-11-17 2021-05-20 Darshan THAKER Systems and methods for analyses of biological samples
EP4717174A3 (en) 2019-12-11 2026-06-17 Magnolia Medical Technologies, Inc. Fluid transfer devices with integrated flow-based assay
IL296312A (en) 2020-03-09 2022-11-01 Berkeley Lights Inc Methods, systems and kits for tests to be performed inside a pen
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CA3189006A1 (en) 2020-09-07 2022-03-10 Matthew Asuka KUBIT Methods of assaying a biological cell
US12669417B2 (en) 2022-03-28 2026-06-30 Bruker Spatial Biology, Inc. Method for assaying a micro-object within a microfluidic device

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251691B1 (en) 1996-04-25 2001-06-26 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1127611A3 (en) * 1999-03-03 2001-11-07 Symyx Technologies, Inc. Chemical processing microsystems with integrated, separation-based reaction product analysis
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
CN100402850C (zh) 1999-06-28 2008-07-16 加利福尼亚技术学院 微型制造的弹性体的阀和泵系统
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
EP1499706A4 (en) 2002-04-01 2010-11-03 Fluidigm Corp MICROFLUIDIC PARTICLE ANALYSIS SYSTEMS
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
EP1615721B1 (en) 2003-04-03 2014-06-18 Fluidigm Corporation Microfluidic devices and methods of using same
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
WO2005072399A2 (en) 2004-01-29 2005-08-11 Massachusetts Institute Of Technology Microscale sorting cytometer
WO2005080606A1 (en) 2004-02-18 2005-09-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
JPWO2005121767A1 (ja) * 2004-05-25 2008-04-10 有限会社フルイド マイクロ流体デバイス及びこれを用いる分析分取装置
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
CN101548004A (zh) 2005-08-19 2009-09-30 加利福尼亚大学董事会 用于诊断学和细胞分析的微流体方法
EP1915467A2 (en) 2005-08-19 2008-04-30 The Regents of the University of California Microfluidic methods for diagnostics and cellular analysis
EP1951742A4 (en) 2005-10-27 2011-06-01 Life Technologies Corp OPTOELECTRONIC SEPARATION OF BIOMOLECULES
US7964078B2 (en) 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
US9040288B2 (en) 2006-03-24 2015-05-26 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
AU2007265628B2 (en) 2006-06-23 2012-12-06 Revvity Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
WO2008147530A1 (en) 2007-05-24 2008-12-04 The Regents Of The University Of California Integrated fluidics devices with magnetic sorting
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
EP2212437A4 (en) 2007-11-07 2011-09-28 Univ British Columbia MICROFLUIDIC DEVICE AND METHOD OF USING THE DEVICE
JP2011516867A (ja) 2008-04-03 2011-05-26 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 細胞、小胞、ナノ粒子およびバイオマーカーを分離および単離するためのエキソビボの多次元システム
EP2279238A2 (en) 2008-04-21 2011-02-02 Cell Kinetics, Ltd. Flat cell carriers with cell traps
CN101275114A (zh) 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
KR100991752B1 (ko) 2008-07-15 2010-11-03 한국과학기술원 단일 평면 광전자 소자를 이용한 미세입자 구동장치 및구동방법
EP2191471A4 (en) 2008-07-28 2013-12-18 Agere Systems Inc SYSTEMS AND METHODS FOR VARIABLE COMPENSATED FLIGHT HEIGHT MEASUREMENT
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
WO2010115167A2 (en) 2009-04-03 2010-10-07 The Regents Of The University Of California Methods and devices for sorting cells and other biological particulates
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (ja) 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
TW201109653A (en) 2009-07-06 2011-03-16 Sony Corp Microfluidic device
CN102472697A (zh) 2009-07-06 2012-05-23 索尼公司 微流体装置
US20110143378A1 (en) 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
EP2578674A4 (en) 2010-05-26 2014-04-30 Tosoh Corp DEVICE FOR FIXING BIOLOGICAL SAMPLES
CN101947124B (zh) * 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
CN104741157B (zh) * 2010-09-14 2017-04-12 加利福尼亚大学董事会 利用微流体截留涡流从异质溶液中分离细胞的装置
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
US9891157B2 (en) * 2010-12-03 2018-02-13 Cellply S.R.L. Microanalysis of cellular function
WO2012072823A1 (en) * 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Rapid screening of monoclonal antibodies
CN116179323A (zh) 2011-05-27 2023-05-30 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
US10626435B2 (en) * 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
TWI512383B (zh) 2012-07-04 2015-12-11 Ind Tech Res Inst 光學感應式介電泳裝置
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
KR102402451B1 (ko) 2013-03-28 2022-05-26 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
KR102113990B1 (ko) 2013-10-22 2020-05-25 버클리 라잇츠, 인크. 생물학적 활성물 분석용 미세 유체 소자
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
KR102221894B1 (ko) * 2013-10-22 2021-03-03 버클리 라잇츠, 인크. 격리 펜을 갖는 미세유체 소자 및 이를 사용한 생물학적 미세 물체를 시험하는 방법
KR102369770B1 (ko) 2014-12-08 2022-03-02 버클리 라잇츠, 인크. 측방향/수직 트랜지스터 구조들을 포함하는 미세유체 디바이스 및 그 제조 및 사용 프로세스
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
CA2982252A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Microfluidic device for culturing biological cells and methods of use therof

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