SG11201602629RA - Microfluidic devices having isolation pens and methods of testing biological micro-objects with same - Google Patents

Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Info

Publication number
SG11201602629RA
SG11201602629RA SG11201602629RA SG11201602629RA SG11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA
Authority
SG
Singapore
Prior art keywords
objects
methods
same
microfluidic devices
biological micro
Prior art date
Application number
SG11201602629RA
Inventor
Eric D Hobbs
Mark P White
J Tanner Nevill
Daniele Malleo
Steven W Short
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52993522&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG11201602629R(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11201602629RA publication Critical patent/SG11201602629RA/en

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652Sorting or classification of particles or molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424Dielectrophoretic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00Immunoassays for the detection of microorganisms
    • G01N2469/20Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201602629RA 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same SG11201602629RA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361996969P 2013-10-22 2013-10-22
US201462058658P 2014-10-01 2014-10-01
PCT/US2014/061837 WO2015061497A1 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US14/520,568 US10010882B2 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Publications (1)

Publication Number Publication Date
SG11201602629RA true SG11201602629RA (en) 2016-05-30

Family

ID=52993522

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201602629RA SG11201602629RA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
SG10202006525PA SG10202006525PA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202006525PA SG10202006525PA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Country Status (9)

Country Link
US (4) US10010882B2 (en)
EP (3) EP3760703A1 (en)
JP (4) JP6557658B2 (en)
KR (4) KR102358356B1 (en)
AU (3) AU2014340089B2 (en)
CA (2) CA3095333C (en)
IL (3) IL283153B2 (en)
SG (2) SG11201602629RA (en)
WO (1) WO2015061497A1 (en)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104508492B (en) 2012-05-25 2018-07-27 北卡罗来纳-查佩尔山大学 Microfluidic device, solid support and correlation technique for reagent
EP3760703A1 (en) 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
GB201320146D0 (en) 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
EP3227025B1 (en) 2014-12-05 2019-03-27 The Regents of The University of California Single-sided light-actuated microfluidic device with integrated mesh ground
CN110918142B (en) 2014-12-08 2022-10-25 伯克利之光生命科技公司 Directional flow actuated microfluidic structures in microfluidic devices and methods of using the same
EP3831482B1 (en) 2014-12-08 2024-01-24 Berkeley Lights, Inc. Microfluidic device comprising lateral/vertical transistor structures
EP3230717B1 (en) 2014-12-09 2021-08-18 Berkeley Lights, Inc. Automated detection and identification of micro-objects in microfluidic devices
CN107206377B (en) 2014-12-09 2020-02-11 伯克利之光生命科技公司 Automated detection of positive assay regions in microfluidic devices
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
AU2015360611B2 (en) 2014-12-10 2021-04-01 Berkeley Lights, Inc. Systems for operating electrokinetic devices
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
AU2016252995B2 (en) 2015-04-22 2020-12-03 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
WO2016172454A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Microfluidic cell structure
JP6805166B2 (en) * 2015-04-22 2020-12-23 バークレー ライツ,インコーポレイテッド Culture station for microfluidic devices
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
US10870111B2 (en) 2015-07-22 2020-12-22 The University Of North Carolina At Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
CN115532189A (en) 2015-11-23 2022-12-30 伯克利之光生命科技公司 In-situ generated microfluidic isolation structure, kit and use method thereof
AU2016365724B2 (en) 2015-12-08 2023-10-12 Berkeley Lights, Inc. Microfluidic devices and kits and methods for use thereof
IL299835A (en) * 2015-12-30 2023-03-01 Berkeley Lights Inc Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
TWI808934B (en) 2015-12-31 2023-07-21 美商伯克利之光生命科技公司 Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
US11420204B2 (en) 2016-01-13 2022-08-23 Duke University Platforms for single cell analysis
TWI756199B (en) 2016-01-15 2022-03-01 美商伯克利之光生命科技公司 Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
WO2017160991A1 (en) 2016-03-16 2017-09-21 Lavieu Gregory G Methods, systems and devices for selection and generation of genome edited clones
EP3922716A1 (en) * 2016-03-17 2021-12-15 Berkeley Lights, Inc. Selection and cloning of t lymphocytes in a microfluidic device
KR102451535B1 (en) * 2016-03-31 2022-10-05 버클리 라잇츠, 인크. Nucleic acid stabilization reagents, kits, and methods of use thereof
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
CN115184315A (en) * 2016-04-15 2022-10-14 伯克利之光生命科技公司 Methods, systems, and kits for in-dock assays
IL263274B2 (en) 2016-05-26 2023-10-01 Berkeley Lights Inc Covalently modified surfaces, kits, and methods of preparation and use
CA3030748C (en) * 2016-07-21 2023-08-29 Berkeley Lights, Inc. Sorting of t lymphocytes in a microfluidic device
KR102650753B1 (en) 2016-10-01 2024-03-26 버클리 라잇츠, 인크. DNA barcode compositions and methods for in situ identification in microfluidic devices
WO2018076024A2 (en) * 2016-10-23 2018-04-26 Berkeley Lights, Inc. Methods for screening b cell lymphocytes
IL267008B2 (en) 2016-12-01 2024-06-01 Berkeley Lights Inc Apparatuses, Systems and Methods for Imaging Micro-Objects
EP3549099A4 (en) 2016-12-01 2020-07-29 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
JP7356351B2 (en) 2016-12-12 2023-10-04 エクセラ・バイオサイエンシーズ・インコーポレイテッド Methods and systems for screening using microcapillary arrays
CN110546495B (en) * 2016-12-30 2022-11-01 加利福尼亚州立大学董事会 Methods for selection and passage of genome editing T cells
US11474103B2 (en) 2017-02-17 2022-10-18 Koniku Inc. Systems for detection
EP3635112A2 (en) 2017-06-06 2020-04-15 Zymergen, Inc. A htp genomic engineering platform for improving fungal strains
SG11202000425QA (en) 2017-07-21 2020-02-27 Berkeley Lights Inc Antigen-presenting synthetic surfaces, covalently functionalized surfaces, activated t cells, and uses thereof
EP3721209B1 (en) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Methods for in-pen assays
WO2019232473A2 (en) 2018-05-31 2019-12-05 Berkeley Lights, Inc. Automated detection and characterization of micro-objects in microfluidic devices
JP2021526799A (en) 2018-06-06 2021-10-11 ザイマージェン インコーポレイテッド Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
WO2020061499A1 (en) 2018-09-21 2020-03-26 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
EP3874265A4 (en) * 2018-11-01 2022-09-28 Berkeley Lights, Inc. Methods for assaying biological cells in a microfluidic device
IT201900002777A1 (en) * 2019-02-26 2020-08-26 Menarini Silicon Biosystems Spa METHOD AND MICROFLUIDIC SYSTEM FOR THE ISOLATION OF PARTICLES
CN114126762B (en) 2019-04-30 2023-01-03 伯克利之光生命科技公司 Methods for encapsulating and assaying cells
IL292993A (en) 2019-11-17 2022-07-01 Berkeley Lights Inc Systems and methods for analyses of biological samples
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE366418T1 (en) 1996-04-25 2007-07-15 Bioarray Solutions Ltd LIGHT-REGULATED, ELECTROKINETIC COMPOSITION OF PARTICLES ON SURFACES
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1106244A3 (en) * 1999-03-03 2001-11-21 Symyx Technologies, Inc. Chemical processing microsystems and controlling reaction conditions in same
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
DK1065378T3 (en) 1999-06-28 2002-07-29 California Inst Of Techn Elastomeric micropump and micro valve systems
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (en) 2001-05-02 2002-11-02 Silicon Biosystems S R L METHOD AND DEVICE FOR THE EXECUTION OF TESTS AND TESTS WITH HIGH PROCESSIVITY AND HIGH BIOLOGICAL VALUE ON CELLS AND / OR COMPOUNDS.
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
EP2666849A3 (en) 2002-04-01 2014-05-28 Fluidigm Corporation Microfluidic particle-analysis systems
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
EP2340890B1 (en) 2003-04-03 2016-10-19 Fluidigm Corporation Method of performimg digital PCR
JP4328168B2 (en) 2003-10-02 2009-09-09 ソニー株式会社 Detection unit for interaction between substances using capillary phenomenon, method using the detection unit, and substrate for bioassay
US7425253B2 (en) 2004-01-29 2008-09-16 Massachusetts Institute Of Technology Microscale sorting cytometer
CN1942590B (en) 2004-02-18 2012-09-05 周小川 Fluidic devices and methods for multiplex chemical and biochemical reactions
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
JPWO2005121767A1 (en) * 2004-05-25 2008-04-10 有限会社フルイド Microfluidic device and analytical fractionation apparatus using the same
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
US9260688B2 (en) 2005-07-07 2016-02-16 The Regents Of The University Of California Methods and apparatus for cell culture array
CN101548004A (en) 2005-08-19 2009-09-30 加利福尼亚大学董事会 Microfluidic methods for diagnostics and cellular analysis
WO2007024701A2 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Microfluidic methods for diagnostics and cellular analysis
WO2007102839A2 (en) 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
US7964078B2 (en) * 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
ES2587007T3 (en) 2006-03-24 2016-10-20 Handylab, Inc. Integrated system to process microfluidic samples, and methods of use thereof
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
EP2041573B1 (en) 2006-06-23 2019-09-04 PerkinElmer Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
US8157434B2 (en) 2007-01-19 2012-04-17 Fluidigm Corporation High efficiency and high precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
ATE554859T1 (en) 2007-05-24 2012-05-15 Univ California INTEGRATED FLUIDIC DEVICES WITH MAGNETIC SORTING
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
WO2009059430A1 (en) 2007-11-07 2009-05-14 The University Of British Columbia Microfluidic device and method of using same
CN103353476B (en) 2008-04-03 2016-05-25 加利福尼亚大学董事会 The ex-vivo multi-dimensional of cell, vesica, nano particle and biomarker separates and emanates
US20110117634A1 (en) 2008-04-21 2011-05-19 Asaf Halamish Flat cell carriers with cell traps
CN101275114A (en) 2008-04-22 2008-10-01 北京大学 Microflow cell culture array and application thereof
KR100991752B1 (en) 2008-07-15 2010-11-03 한국과학기술원 Apparatus and Method for Microparticle Manipulation Using Single Planar Optoelectronic Device
JP5623399B2 (en) 2008-07-28 2014-11-12 アギア システムズ エルエルシーAgere Systems LLC Variable compensation flying height measurement system and method
GB2464300A (en) * 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
JP2012522518A (en) * 2009-04-03 2012-09-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Method and apparatus for sorting cells and bioparticles
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (en) 2009-06-19 2011-01-06 Univ Of Tokyo Method for operating particle and micro fluid device
WO2011005778A1 (en) 2009-07-06 2011-01-13 Sony Corporation Microfluidic device
CN102472697A (en) 2009-07-06 2012-05-23 索尼公司 Microfluidic device
US20110143378A1 (en) 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
JPWO2011149032A1 (en) 2010-05-26 2013-07-25 東ソー株式会社 Biological sample fixing device
CN101947124B (en) 2010-06-25 2012-07-04 博奥生物有限公司 Integrated microfluidic chip device and using method thereof
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
CA2809877C (en) 2010-09-14 2021-11-23 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
EP2646798B1 (en) * 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
US10571475B2 (en) * 2010-12-03 2020-02-25 Cellply S.R.L. Rapid screening of monoclonal antibodies
CN108424834A (en) 2011-05-27 2018-08-21 不列颠哥伦比亚大学 Micro-current controlled cell capture for high throughput analysis and analytical equipment
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
US10626435B2 (en) * 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
TWI512383B (en) 2012-07-04 2015-12-11 Ind Tech Res Inst Optically-induced dielectrophoresis device
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
WO2014153651A1 (en) 2013-03-28 2014-10-02 The University Of British Columbia Microfluidic devices and methods for use thereof in multicellular assays of secretion
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3760703A1 (en) * 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
JP6465878B2 (en) 2013-10-22 2019-02-06 バークレー ライツ,インコーポレイテッド Microfluidic device for assaying biological activity
EP3831482B1 (en) 2014-12-08 2024-01-24 Berkeley Lights, Inc. Microfluidic device comprising lateral/vertical transistor structures
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
WO2016172454A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Microfluidic cell structure

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