JP2016535253A - 体積試料の切断 - Google Patents
体積試料の切断 Download PDFInfo
- Publication number
- JP2016535253A JP2016535253A JP2016522749A JP2016522749A JP2016535253A JP 2016535253 A JP2016535253 A JP 2016535253A JP 2016522749 A JP2016522749 A JP 2016522749A JP 2016522749 A JP2016522749 A JP 2016522749A JP 2016535253 A JP2016535253 A JP 2016535253A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- plate
- blade
- section
- sections
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005520 cutting process Methods 0.000 title claims description 24
- 239000007788 liquid Substances 0.000 claims abstract description 150
- 238000000034 method Methods 0.000 claims description 79
- 230000004913 activation Effects 0.000 claims description 34
- 238000003384 imaging method Methods 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 19
- 238000005259 measurement Methods 0.000 claims description 18
- 239000002985 plastic film Substances 0.000 claims description 8
- 229920006255 plastic film Polymers 0.000 claims description 8
- 230000005499 meniscus Effects 0.000 claims description 6
- 230000003247 decreasing effect Effects 0.000 claims description 4
- 238000001125 extrusion Methods 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 239000000523 sample Substances 0.000 description 315
- 238000003860 storage Methods 0.000 description 20
- 238000004627 transmission electron microscopy Methods 0.000 description 18
- 239000011521 glass Substances 0.000 description 14
- 238000010894 electron beam technology Methods 0.000 description 12
- -1 plate Substances 0.000 description 6
- 230000005540 biological transmission Effects 0.000 description 5
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
- 230000000712 assembly Effects 0.000 description 4
- 238000000429 assembly Methods 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000012876 topography Methods 0.000 description 2
- 241000254032 Acrididae Species 0.000 description 1
- 241000243818 Annelida Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229910000881 Cu alloy Inorganic materials 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 229910000990 Ni alloy Inorganic materials 0.000 description 1
- 229910000639 Spring steel Inorganic materials 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 238000001350 scanning transmission electron microscopy Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
- G01N2001/065—Drive details
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T83/00—Cutting
- Y10T83/04—Processes
- Y10T83/0448—With subsequent handling [i.e., of product]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T83/00—Cutting
- Y10T83/141—With means to monitor and control operation [e.g., self-regulating means]
- Y10T83/148—Including means to correct the sensed operation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T83/00—Cutting
- Y10T83/647—With means to convey work relative to tool station
- Y10T83/6572—With additional mans to engage work and orient it relative to tool station
- Y10T83/6577—With means to adjust additional means
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361900262P | 2013-11-05 | 2013-11-05 | |
| US61/900,262 | 2013-11-05 | ||
| PCT/US2014/064151 WO2015069783A1 (en) | 2013-11-05 | 2014-11-05 | Sectioning volume samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2016535253A true JP2016535253A (ja) | 2016-11-10 |
| JP2016535253A5 JP2016535253A5 (enExample) | 2016-12-28 |
Family
ID=53042042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016522749A Pending JP2016535253A (ja) | 2013-11-05 | 2014-11-05 | 体積試料の切断 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US10054518B2 (enExample) |
| EP (1) | EP3066447A4 (enExample) |
| JP (1) | JP2016535253A (enExample) |
| WO (1) | WO2015069783A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017169479A (ja) * | 2016-03-23 | 2017-09-28 | オリンパス株式会社 | 観察装置、測定システム及び培養容器 |
| TWI611170B (zh) * | 2016-10-05 | 2018-01-11 | 閤康生物科技股份有限公司 | 樣品製備系統及製備方法 |
| DE102017129537A1 (de) * | 2017-12-12 | 2019-06-13 | Karlsruher Institut für Technologie | Mikrotom und Verfahren zum Herstellen von Dünnschnitten aus einer Probe mit Hilfe eines Mikrotoms |
| EP4116696A1 (en) * | 2021-07-06 | 2023-01-11 | Leica Mikrosysteme GmbH | Method for preparing a microscopic sample for examination in an electron microscope and embedding mould for use therein |
| EP4269983B1 (en) | 2022-04-26 | 2025-06-11 | Leica Mikrosysteme GmbH | Microtome system and corresponding method |
| EP4336165A1 (en) * | 2022-09-06 | 2024-03-13 | Leica Mikrosysteme GmbH | Sample holder for holding a sample carrier, loading station, knife holder, microtome and corresponding methods |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3832923A (en) * | 1972-01-01 | 1974-09-03 | Schwarzer Co | Method for the production of microtome slices and device for the implementation of the method |
| DE2265184A1 (de) * | 1972-01-05 | 1976-09-02 | Jung Zweigniederlassung Der Fr | Mikrotom fuer weichschnitte |
| US4697489A (en) | 1984-07-05 | 1987-10-06 | Kim George A | Ultramicrotome tool |
| AT399227B (de) | 1990-04-11 | 1995-04-25 | Sitte Hellmuth | Vorrichtung zur steuerung des antriebes sowie des vorschubes eines mikrotoms, insbesondere ultramikrotoms |
| US5551326A (en) * | 1994-12-15 | 1996-09-03 | Wisconsin Alumni Research Foundation | Adhesive-less microtome boat |
| US5752425A (en) | 1995-12-25 | 1998-05-19 | Chuo Precision Industrial Co., Ltd. | Microtome |
| DE19645107C2 (de) | 1996-11-01 | 1999-06-24 | Leica Ag | Mikrotom mit einem oszillierenden Messer |
| JPH10197418A (ja) * | 1997-01-10 | 1998-07-31 | Chuo Seiki Kk | ミクロトーム |
| US20040107807A1 (en) * | 1997-12-19 | 2004-06-10 | Daniel Studer | Ultramicrotome device |
| DE19911173C2 (de) * | 1999-03-12 | 2002-01-31 | Leica Microsystems | Mikrotom mit einem motorischen Zustellantrieb |
| JP3576136B2 (ja) | 2001-11-30 | 2004-10-13 | 堂阪イーエム株式会社 | 試験片切断装置 |
| US7677289B2 (en) * | 2004-07-08 | 2010-03-16 | President And Fellows Of Harvard College | Methods and apparatuses for the automated production, collection, handling, and imaging of large numbers of serial tissue sections |
| CN101500767B (zh) * | 2006-09-06 | 2010-12-15 | 孔健强 | 用于切割新鲜组织切片的方法和设备 |
| US8869666B2 (en) * | 2011-03-24 | 2014-10-28 | Sakura Finetek U.S.A., Inc. | Microtome with surface orientation sensor to sense orientation of surface of sample |
-
2014
- 2014-11-05 JP JP2016522749A patent/JP2016535253A/ja active Pending
- 2014-11-05 EP EP14859472.4A patent/EP3066447A4/en not_active Withdrawn
- 2014-11-05 WO PCT/US2014/064151 patent/WO2015069783A1/en not_active Ceased
- 2014-11-05 US US14/533,904 patent/US10054518B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015069783A1 (en) | 2015-05-14 |
| EP3066447A4 (en) | 2017-06-07 |
| EP3066447A1 (en) | 2016-09-14 |
| US10054518B2 (en) | 2018-08-21 |
| US20150135917A1 (en) | 2015-05-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20161110 |