JP2016525890A - 遺伝的不妊性動物 - Google Patents

遺伝的不妊性動物 Download PDF

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Publication number
JP2016525890A
JP2016525890A JP2016516661A JP2016516661A JP2016525890A JP 2016525890 A JP2016525890 A JP 2016525890A JP 2016516661 A JP2016516661 A JP 2016516661A JP 2016516661 A JP2016516661 A JP 2016516661A JP 2016525890 A JP2016525890 A JP 2016525890A
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gene
animal
cell
animals
sperm
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Japanese (ja)
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JP2016525890A5 (enExample
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ダニエル・エフ・カールソン
スコット・シー・ファーレンクラッグ
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Recombinetics Inc
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Recombinetics Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A01K2217/077—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out heterozygous knock out animals displaying phenotype
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/103—Ovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
JP2016516661A 2013-05-31 2014-04-29 遺伝的不妊性動物 Pending JP2016525890A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361829656P 2013-05-31 2013-05-31
US61/829,656 2013-05-31
US201361870558P 2013-08-27 2013-08-27
US61/870,558 2013-08-27
PCT/US2014/035847 WO2014193583A2 (en) 2013-05-31 2014-04-29 Genetically sterile animals

Publications (2)

Publication Number Publication Date
JP2016525890A true JP2016525890A (ja) 2016-09-01
JP2016525890A5 JP2016525890A5 (enExample) 2017-06-15

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JP2016516661A Pending JP2016525890A (ja) 2013-05-31 2014-04-29 遺伝的不妊性動物

Country Status (13)

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US (2) US20140359796A1 (enExample)
EP (1) EP3004345A4 (enExample)
JP (1) JP2016525890A (enExample)
KR (1) KR20160013219A (enExample)
CN (1) CN105473714A (enExample)
AP (1) AP2015008963A0 (enExample)
AU (1) AU2014272106A1 (enExample)
BR (1) BR112015029904A2 (enExample)
CA (1) CA2913411A1 (enExample)
MX (1) MX2015016385A (enExample)
PH (1) PH12015502640A1 (enExample)
RU (1) RU2015156801A (enExample)
WO (1) WO2014193583A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
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JP2022502064A (ja) * 2018-10-12 2022-01-11 ザ ユニバーシティー コート オブ ザ ユニバーシティー オブ エジンバラThe University Court Of The University Of Edinburgh 遺伝子改変不妊鳥類およびその再構成方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
ES2962492T3 (es) 2013-10-25 2024-03-19 Livestock Improvement Corporation Ltd Marcadores genéticos y usos de los mismos
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
US9745561B2 (en) * 2014-01-08 2017-08-29 Iowa State University Research Foundation, Inc. Genetic test and genetic basis for SCID in pigs
BR122023023211A2 (pt) 2014-04-28 2024-01-23 Recombinetics, Inc. Método de fazer edições de genes multiplex em uma célula de vertebrado ou de embrião primário não-humano
EP3169778B1 (en) 2014-07-14 2023-10-25 Washington State University Nanos knock-out that ablates germline cells
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
KR102636332B1 (ko) * 2015-04-08 2024-02-14 내셔날 페더레이션 오브 애그리컬쳐 코오퍼레이티브 어소우시에이션스 이개체 유래의 배우자를 생산하는 비인간 대형 포유 동물 또는 어류의 작출 방법
CN108368502B (zh) 2015-06-03 2022-03-18 内布拉斯加大学董事委员会 使用单链dna的dna编辑
US20170099813A1 (en) * 2015-10-07 2017-04-13 Recombinetics, Inc. Method of generating sterile terminal sires in livestock and animals produced thereby
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
CN105543257A (zh) * 2016-01-18 2016-05-04 安徽农业大学 一种pAPN基因定点修饰猪
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
IL306092A (en) 2017-03-23 2023-11-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3498096A1 (en) * 2017-12-18 2019-06-19 IMV Technologies Method for increasing the lifespan and motility of animal sperm using an inhibitor of slo3 potassium channel
CN110184296B (zh) * 2018-02-07 2023-06-09 上海大学 一种制备雌雄均高度不育的鳞翅目昆虫的方法及其核酸构建物
RU2020131565A (ru) * 2018-02-26 2022-03-29 Эгдженетикс, Инк. Материалы и способы для предотвращения передачи конкретной хромосомы
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
WO2020047514A2 (en) * 2018-08-31 2020-03-05 Recombinetics, Inc. Production method for animal models with disease associated phenotypes
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN109837347B (zh) * 2019-01-02 2021-01-29 华中农业大学 Catsper4基因第三外显子的snp作为猪精液品质性状的遗传标记
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
BR112021020349A2 (pt) * 2019-04-09 2021-12-14 Mayo Found Medical Education & Res Peixe estéril
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN111378663B (zh) * 2020-03-18 2021-07-13 吉林省农业科学院 lncRNA SFR1及其应用、调控卵泡发育的产品和方法
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
CN111549032A (zh) * 2020-05-26 2020-08-18 肇庆学院 一种诱导型精原细胞Tubb4b基因敲除细胞系的构建方法
TW202241259A (zh) * 2020-12-31 2022-11-01 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） 不育禽類胚胎、其產生及用途
KR102285906B1 (ko) 2021-02-18 2021-08-04 주식회사 엔에이피 수생태계 교란어종 관리를 위한 큰입배스 불임 유도용 조성물
IL282597B (en) * 2021-04-22 2022-08-01 B G Negev Technologies And Applications Ltd At Ben Gurion Univ Birds for producing female hatchling and methods of producing same
CN113558012A (zh) * 2021-08-05 2021-10-29 陈米米 一种哺乳动物繁育雌性多胎的方法
WO2024042515A1 (en) * 2022-08-22 2024-02-29 Ramot At Tel-Aviv University Ltd. Genetically engineered eukaryotic organisms, systems and methods for in vivo biasing sex-ratio of populations
CN116064541B (zh) * 2022-10-21 2025-03-18 华南农业大学 一种基于CRISPR/Cas9系统敲除猪细胞Y染色体的方法
KR20250033452A (ko) 2023-08-30 2025-03-10 서울대학교산학협력단 Cyp17a1 형질전환 비만 및 불임치료제 개발 모델 제조방법 및 용도
CN116884488B (zh) * 2023-09-07 2023-11-24 中国医学科学院基础医学研究所 一种男性不育标志物的筛查方法、系统及设备
CN118910165A (zh) * 2024-10-11 2024-11-08 上海南方模式生物科技股份有限公司 一种cxcl16基因人源化动物模型的构建方法及应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002543814A (ja) * 1999-05-13 2002-12-24 セダーシナイ メディカル センター トランスジェニック種の発生と遺伝子療法用の雄性生殖細胞の遺伝的修飾法
US20050125853A1 (en) * 2002-03-22 2005-06-09 St. Jude Children's Resarch Hospital Method for generating genetically modified animals
US20120180151A1 (en) * 2009-08-28 2012-07-12 Pawal Pelczar Genetic vasectomy by overexpression of prm1-egfp fusion protein in spermatids
WO2012116274A2 (en) * 2011-02-25 2012-08-30 Recombinetics, Inc. Genetically modified animals and methods for making the same
WO2012158985A2 (en) * 2011-05-17 2012-11-22 Transposagen Biopharmaceuticals, Inc. Methods for site-specific genetic modification in spermatogonial stem cells using zinc finger nuclease (zfn) for the creation of model organisms
JP2013500018A (ja) * 2009-07-24 2013-01-07 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー ゲノム編集のための方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101024829A (zh) * 2000-02-24 2007-08-29 马萨诸塞大学 只生育单一性别后代的哺乳动物的生产
US20050081256A1 (en) * 2001-03-22 2005-04-14 Forsberg Erik J. Sex- specific selection of sperm from transgenic animals
US8735156B2 (en) * 2008-12-01 2014-05-27 The Board Of Regents Of The University Of Texas System Production and use of rat spermatogonial stem cell lines
US20140123330A1 (en) * 2012-10-30 2014-05-01 Recombinetics, Inc. Control of sexual maturation in animals
US20140359795A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetic techniques for making animals with sortable sperm

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002543814A (ja) * 1999-05-13 2002-12-24 セダーシナイ メディカル センター トランスジェニック種の発生と遺伝子療法用の雄性生殖細胞の遺伝的修飾法
US20050125853A1 (en) * 2002-03-22 2005-06-09 St. Jude Children's Resarch Hospital Method for generating genetically modified animals
JP2013500018A (ja) * 2009-07-24 2013-01-07 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー ゲノム編集のための方法
US20120180151A1 (en) * 2009-08-28 2012-07-12 Pawal Pelczar Genetic vasectomy by overexpression of prm1-egfp fusion protein in spermatids
WO2012116274A2 (en) * 2011-02-25 2012-08-30 Recombinetics, Inc. Genetically modified animals and methods for making the same
WO2012158985A2 (en) * 2011-05-17 2012-11-22 Transposagen Biopharmaceuticals, Inc. Methods for site-specific genetic modification in spermatogonial stem cells using zinc finger nuclease (zfn) for the creation of model organisms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JANET HAUSCHILD, ET AL.: "Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases.", PNAS, vol. 108, JPN6018015645, 2011, pages 12013 - 12017, ISSN: 0003789110 *
MATTEO RUGGIU, ET AL.: "The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis.", NATURE, vol. 389, JPN6018015647, 1997, pages 73 - 77, ISSN: 0003789111 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022502064A (ja) * 2018-10-12 2022-01-11 ザ ユニバーシティー コート オブ ザ ユニバーシティー オブ エジンバラThe University Court Of The University Of Edinburgh 遺伝子改変不妊鳥類およびその再構成方法
JP7535792B2 (ja) 2018-10-12 2024-08-19 ザ ユニバーシティー コート オブ ザ ユニバーシティー オブ エジンバラ 遺伝子改変不妊鳥類およびその再構成方法

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BR112015029904A2 (pt) 2017-09-26
EP3004345A4 (en) 2017-02-15
EP3004345A2 (en) 2016-04-13
US20190335725A1 (en) 2019-11-07
AU2014272106A1 (en) 2016-01-21
WO2014193583A2 (en) 2014-12-04
CA2913411A1 (en) 2014-12-04
CN105473714A (zh) 2016-04-06
PH12015502640A1 (en) 2016-03-07
AP2015008963A0 (en) 2016-01-31
KR20160013219A (ko) 2016-02-03
NZ715540A (en) 2021-04-30
RU2015156801A (ru) 2017-07-06
MX2015016385A (es) 2016-04-11
US20140359796A1 (en) 2014-12-04
WO2014193583A3 (en) 2015-01-22

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