BR112015029904A2 - animais geneticamente estéreis - Google Patents

animais geneticamente estéreis

Info

Publication number
BR112015029904A2
BR112015029904A2 BR112015029904A BR112015029904A BR112015029904A2 BR 112015029904 A2 BR112015029904 A2 BR 112015029904A2 BR 112015029904 A BR112015029904 A BR 112015029904A BR 112015029904 A BR112015029904 A BR 112015029904A BR 112015029904 A2 BR112015029904 A2 BR 112015029904A2
Authority
BR
Brazil
Prior art keywords
producing
methods
target gene
animal
animals
Prior art date
Application number
BR112015029904A
Other languages
English (en)
Inventor
f carlson Daniel
C Fahrenkrug Scott
Original Assignee
Recombinetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Recombinetics Inc filed Critical Recombinetics Inc
Publication of BR112015029904A2 publication Critical patent/BR112015029904A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A01K2217/077—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out heterozygous knock out animals displaying phenotype
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/103—Ovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)

Abstract

resumo animais geneticamente estéreis um animal de gado geneticamente modificado, e métodos para produzir e utilizar o mesmo, o animal, compreendendo uma modificação genética para interromper um gene alvo envolvidos na gametogénese seletivamente, em que a ruptura do gene alvo previne a formação de gametas funcionais do animal. animais que criam descendência com doadores genética, e métodos para produzir e utilizar a mesma. as células, e os métodos para produzir e utilizar as células, com uma modificação genética para interromper um gene alvo envolvidos na gametogénese seletivamente.
BR112015029904A 2013-05-31 2014-04-29 animais geneticamente estéreis BR112015029904A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361829656P 2013-05-31 2013-05-31
US201361870558P 2013-08-27 2013-08-27
PCT/US2014/035847 WO2014193583A2 (en) 2013-05-31 2014-04-29 Genetically sterile animals

Publications (1)

Publication Number Publication Date
BR112015029904A2 true BR112015029904A2 (pt) 2017-09-26

Family

ID=51986779

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015029904A BR112015029904A2 (pt) 2013-05-31 2014-04-29 animais geneticamente estéreis

Country Status (13)

Country Link
US (2) US20140359796A1 (pt)
EP (1) EP3004345A4 (pt)
JP (1) JP2016525890A (pt)
KR (1) KR20160013219A (pt)
CN (1) CN105473714A (pt)
AP (1) AP2015008963A0 (pt)
AU (1) AU2014272106A1 (pt)
BR (1) BR112015029904A2 (pt)
CA (1) CA2913411A1 (pt)
MX (1) MX2015016385A (pt)
PH (1) PH12015502640A1 (pt)
RU (1) RU2015156801A (pt)
WO (1) WO2014193583A2 (pt)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
BR112016009194B1 (pt) 2013-10-25 2022-05-24 Livestock Improvement Corporation Limited Métodos in vitro para selecionar uma variação em um gene do receptor de prolactina (prlr), produzir uma célula ou embrião de animal não humano, gerar um animal não humano e selecionar uma variação em um gene do prlr
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US9745561B2 (en) * 2014-01-08 2017-08-29 Iowa State University Research Foundation, Inc. Genetic test and genetic basis for SCID in pigs
US20170142942A1 (en) * 2014-07-14 2017-05-25 Washington State University Nanos knock-out that ablates germline cells
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
WO2016163386A1 (ja) * 2015-04-08 2016-10-13 全国農業協同組合連合会 異個体由来の配偶子を生産する非ヒト大型哺乳動物又は魚類の作出方法
EP3303585A4 (en) 2015-06-03 2018-10-31 Board of Regents of the University of Nebraska Dna editing using single-stranded dna
WO2017062756A1 (en) * 2015-10-07 2017-04-13 Recombinetics, Inc. Method of generating sterile terminal sires in livestock and animals produced thereby
JP7067793B2 (ja) 2015-10-23 2022-05-16 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基編集因子およびその使用
CN105543257A (zh) * 2016-01-18 2016-05-04 安徽农业大学 一种pAPN基因定点修饰猪
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
EP3592777A1 (en) 2017-03-10 2020-01-15 President and Fellows of Harvard College Cytosine to guanine base editor
KR20190130613A (ko) 2017-03-23 2019-11-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019079347A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. USES OF BASIC EDITORS ADENOSINE
EP3498096A1 (en) * 2017-12-18 2019-06-19 IMV Technologies Method for increasing the lifespan and motility of animal sperm using an inhibitor of slo3 potassium channel
CN110184296B (zh) * 2018-02-07 2023-06-09 上海大学 一种制备雌雄均高度不育的鳞翅目昆虫的方法及其核酸构建物
CN111787791A (zh) * 2018-02-26 2020-10-16 Ag遗传学股份有限公司 用于防止特定染色体的传播的材料和方法
WO2020047514A2 (en) * 2018-08-31 2020-03-05 Recombinetics, Inc. Production method for animal models with disease associated phenotypes
GB201816633D0 (en) * 2018-10-12 2018-11-28 Univ Edinburgh Genetically modified avians and method for the reconstitution thereof
CN109837347B (zh) * 2019-01-02 2021-01-29 华中农业大学 Catsper4基因第三外显子的snp作为猪精液品质性状的遗传标记
EP3942040A1 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
CN111378663B (zh) * 2020-03-18 2021-07-13 吉林省农业科学院 lncRNA SFR1及其应用、调控卵泡发育的产品和方法
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
CN111549032A (zh) * 2020-05-26 2020-08-18 肇庆学院 一种诱导型精原细胞Tubb4b基因敲除细胞系的构建方法
KR102285906B1 (ko) 2021-02-18 2021-08-04 주식회사 엔에이피 수생태계 교란어종 관리를 위한 큰입배스 불임 유도용 조성물
IL282597B (en) * 2021-04-22 2022-08-01 B G Negev Technologies And Applications Ltd At Ben Gurion Univ Birds for creating female offspring and methods for creating them
CN113558012A (zh) * 2021-08-05 2021-10-29 陈米米 一种哺乳动物繁育雌性多胎的方法
WO2024042515A1 (en) * 2022-08-22 2024-02-29 Ramot At Tel-Aviv University Ltd. Genetically engineered eukaryotic organisms, systems and methods for in vivo biasing sex-ratio of populations
CN116884488B (zh) * 2023-09-07 2023-11-24 中国医学科学院基础医学研究所 一种男性不育标志物的筛查方法、系统及设备

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000069257A2 (en) * 1999-05-13 2000-11-23 Cedars-Sinai Medical Center Genetic modification of male germ cells for generation of transgenic species and genetic therapies
CN1291010C (zh) * 2000-02-24 2006-12-20 马萨诸塞大学 只生育单一性别后代的哺乳动物的生产
WO2002077637A1 (en) * 2001-03-22 2002-10-03 Infigen, Inc. Sex-specific selection of sperm from transgenic animals
AU2003213813A1 (en) * 2002-03-22 2003-10-13 St. Jude Children's Research Hospital Improved method for generating genetically modified animals
WO2010065550A2 (en) * 2008-12-01 2010-06-10 Board Of Regents University Of Texas System Production and use of rat spermatogonial stem cell lines
AU2010275432A1 (en) * 2009-07-24 2012-02-02 Sigma-Aldrich Co. Llc. Method for genome editing
US8633348B2 (en) * 2009-08-28 2014-01-21 Novartis Forschungsstiftung, Zweigniederlassung, Friedrich Miescher Institute For Biomedical Research Genetic vasectomy by overexpression of PRML-EGFP fusion protein in spermatids
AU2012222144B2 (en) * 2011-02-25 2017-04-27 Recombinetics, Inc. Genetically modified animals and methods for making the same
WO2012158985A2 (en) * 2011-05-17 2012-11-22 Transposagen Biopharmaceuticals, Inc. Methods for site-specific genetic modification in spermatogonial stem cells using zinc finger nuclease (zfn) for the creation of model organisms
AP2015008495A0 (en) * 2012-10-30 2015-05-31 Ct For Aquaculture Technologies Inc Control of sexual maturation in animals
US20140359795A1 (en) * 2013-05-31 2014-12-04 Recombinetics, Inc. Genetic techniques for making animals with sortable sperm

Also Published As

Publication number Publication date
WO2014193583A2 (en) 2014-12-04
KR20160013219A (ko) 2016-02-03
NZ715540A (en) 2021-04-30
EP3004345A4 (en) 2017-02-15
PH12015502640A1 (en) 2016-03-07
EP3004345A2 (en) 2016-04-13
CN105473714A (zh) 2016-04-06
AP2015008963A0 (en) 2016-01-31
RU2015156801A (ru) 2017-07-06
US20140359796A1 (en) 2014-12-04
MX2015016385A (es) 2016-04-11
CA2913411A1 (en) 2014-12-04
WO2014193583A3 (en) 2015-01-22
JP2016525890A (ja) 2016-09-01
AU2014272106A1 (en) 2016-01-21
US20190335725A1 (en) 2019-11-07

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B350 Update of information on the portal [chapter 15.35 patent gazette]
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