JP2016515399A5 - - Google Patents
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- JP2016515399A5 JP2016515399A5 JP2016508291A JP2016508291A JP2016515399A5 JP 2016515399 A5 JP2016515399 A5 JP 2016515399A5 JP 2016508291 A JP2016508291 A JP 2016508291A JP 2016508291 A JP2016508291 A JP 2016508291A JP 2016515399 A5 JP2016515399 A5 JP 2016515399A5
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- JP
- Japan
- Prior art keywords
- cell
- seq
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- designer
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cell Anatomy 0.000 claims description 165
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 59
- 229920001184 polypeptide Polymers 0.000 claims description 57
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 57
- 238000000034 method Methods 0.000 claims description 56
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 45
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims description 38
- 102000005751 Alcohol Oxidoreductases Human genes 0.000 claims description 37
- 108010031132 Alcohol Oxidoreductases Proteins 0.000 claims description 37
- 125000000217 alkyl group Chemical group 0.000 claims description 34
- 230000000694 effects Effects 0.000 claims description 34
- 241000588724 Escherichia coli Species 0.000 claims description 29
- 150000001875 compounds Chemical class 0.000 claims description 28
- 210000000805 cytoplasm Anatomy 0.000 claims description 24
- 239000011541 reaction mixture Substances 0.000 claims description 24
- -1 CF 3 Inorganic materials 0.000 claims description 20
- ZAJNMXDBJKCCAT-YFKPBYRVSA-N ethyl (3s)-4-chloro-3-hydroxybutanoate Chemical compound CCOC(=O)C[C@H](O)CCl ZAJNMXDBJKCCAT-YFKPBYRVSA-N 0.000 claims description 20
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical group C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims description 18
- OHLRLMWUFVDREV-UHFFFAOYSA-N ethyl 4-chloro-3-oxobutanoate Chemical compound CCOC(=O)CC(=O)CCl OHLRLMWUFVDREV-UHFFFAOYSA-N 0.000 claims description 18
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 17
- 239000003960 organic solvent Substances 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 150000002576 ketones Chemical class 0.000 claims description 12
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 claims description 10
- 239000007853 buffer solution Substances 0.000 claims description 9
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 239000013598 vector Substances 0.000 claims description 8
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 229940050410 gluconate Drugs 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 230000003647 oxidation Effects 0.000 claims description 6
- 238000007254 oxidation reaction Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000002157 polynucleotide Substances 0.000 claims description 4
- 102000040430 polynucleotide Human genes 0.000 claims description 4
- 108091033319 polynucleotide Proteins 0.000 claims description 4
- 101710088194 Dehydrogenase Proteins 0.000 claims description 3
- 239000013604 expression vector Substances 0.000 claims description 3
- 150000002191 fatty alcohols Chemical class 0.000 claims description 3
- 238000003259 recombinant expression Methods 0.000 claims description 3
- 241000511343 Chondrostoma nasus Species 0.000 claims 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims 1
- 102100022624 Glucoamylase Human genes 0.000 claims 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 18
- DURPTKYDGMDSBL-UHFFFAOYSA-N 1-butoxybutane Chemical compound CCCCOCCCC DURPTKYDGMDSBL-UHFFFAOYSA-N 0.000 description 12
- CXBDYQVECUFKRK-UHFFFAOYSA-N 1-methoxybutane Chemical compound CCCCOC CXBDYQVECUFKRK-UHFFFAOYSA-N 0.000 description 6
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 6
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 6
- 0 *Cc1c(*)c(*)c(*)c(N)c1* Chemical compound *Cc1c(*)c(*)c(*)c(N)c1* 0.000 description 2
- WHBMMWSBFZVSSR-UHFFFAOYSA-M 3-hydroxybutyrate Chemical compound CC(O)CC([O-])=O WHBMMWSBFZVSSR-UHFFFAOYSA-M 0.000 description 2
- WHBMMWSBFZVSSR-UHFFFAOYSA-N R3HBA Natural products CC(O)CC(O)=O WHBMMWSBFZVSSR-UHFFFAOYSA-N 0.000 description 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1146DE2013 | 2013-04-17 | ||
| IN1146/DEL/2013 | 2013-04-17 | ||
| PCT/IN2014/000247 WO2014170917A1 (en) | 2013-04-17 | 2014-04-17 | Designer cells for enantioselective reduction of ketones and use thereof in efficient production of enantioenriched alcohols |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016515399A JP2016515399A (ja) | 2016-05-30 |
| JP2016515399A5 true JP2016515399A5 (enExample) | 2016-11-17 |
| JP6330028B2 JP6330028B2 (ja) | 2018-05-23 |
Family
ID=51062858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016508291A Expired - Fee Related JP6330028B2 (ja) | 2013-04-17 | 2014-04-17 | ケトンのエナンチオ選択性還元のためのデザイナー細胞及びエナンチオ濃縮アルコールの効率的製造におけるその使用 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9790524B2 (enExample) |
| EP (1) | EP2986724B1 (enExample) |
| JP (1) | JP6330028B2 (enExample) |
| KR (1) | KR101876172B1 (enExample) |
| CN (1) | CN105358687B (enExample) |
| WO (1) | WO2014170917A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107326049A (zh) * | 2016-04-28 | 2017-11-07 | 尚科生物医药（上海）有限公司 | 重组酮还原酶在制备(r)-3,5-二（三氟甲基）苯乙醇中的应用 |
| CN106279374A (zh) * | 2016-08-26 | 2017-01-04 | 深圳市职业病防治院 | 一种与铅离子结合的多肽及其应用 |
| CN106399398B (zh) * | 2016-11-16 | 2024-07-09 | 上海医药工业研究院 | (r)-3,5-双(三氟甲基)苯乙醇的生物制备方法 |
| CN107794282B (zh) * | 2017-11-20 | 2020-12-25 | 浙江工业大学 | 一种克唑替尼手性中间体的制备方法及菌株 |
| CN112941124B (zh) * | 2021-02-09 | 2023-12-29 | 江苏阿尔法药业股份有限公司 | 一种全细胞催化制备依利格鲁司特中间体的方法 |
| CN113789353B (zh) * | 2021-09-25 | 2024-03-08 | 复旦大学 | (2s,3s)-2-氯-3-羟基酯类化合物的制备方法 |
| CN115927138A (zh) * | 2022-08-09 | 2023-04-07 | 江南大学 | 一种基于表面展示羰基还原酶的全细胞催化合成2-甲基苯甲酮的方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4710468A (en) | 1983-10-24 | 1987-12-01 | Sigma-Tau Industrie Pharmaceutiche Riunite S.P.A. | Process for preparing L-carnitine and chemical intermediates employed therein |
| JPS62126997A (ja) | 1985-11-28 | 1987-06-09 | Nippon Synthetic Chem Ind Co Ltd:The | 光学活性のγ−ハロ−βヒドロキシ酪酸エステルの製造法 |
| US5413921A (en) | 1992-05-28 | 1995-05-09 | Ajinomoto Co., Ltd. | Method of the production of (s)-gamma-halogenated-γ-hydroxybutyric acid esters |
| JP3155107B2 (ja) | 1993-01-12 | 2001-04-09 | ダイセル化学工業株式会社 | 光学活性４−ハロ−３−ヒドロキシ酪酸エステルの製造方法 |
| JPH08336393A (ja) | 1995-04-13 | 1996-12-24 | Mitsubishi Chem Corp | 光学活性なγ−置換−β−ヒドロキシ酪酸エステルの製造法 |
| US5891685A (en) | 1996-06-03 | 1999-04-06 | Mitsubishi Chemical Corporation | Method for producing ester of (S)-γ-halogenated-β-hydroxybutyric acid |
| US7629157B2 (en) | 2003-08-11 | 2009-12-08 | Codexis, Inc. | Ketoreductase polypeptides and related polynucleotides |
| MXPA06001719A (es) | 2003-08-11 | 2006-05-19 | Codexis Inc | Polipeptidos de glucosa deshidrogenasa mejorados, y polinucleotidos relacionados. |
| KR100743973B1 (ko) * | 2006-04-27 | 2007-07-30 | 한국과학기술원 | 목적단백질 및 코펙터를 재생시키는 효소를 동시에 세포표면에 표면발현시키는 방법 |
| US20090004720A1 (en) * | 2007-06-26 | 2009-01-01 | Ling Hua | Smart Biocatalysts For Organic Synthesis |
| WO2010151593A1 (en) * | 2009-06-23 | 2010-12-29 | Rutgers, The State University Of New Jersey | Sterospecific carbonyl reductases |
-
2014
- 2014-04-17 CN CN201480022301.2A patent/CN105358687B/zh not_active Expired - Fee Related
- 2014-04-17 JP JP2016508291A patent/JP6330028B2/ja not_active Expired - Fee Related
- 2014-04-17 US US14/785,286 patent/US9790524B2/en not_active Expired - Fee Related
- 2014-04-17 KR KR1020157025390A patent/KR101876172B1/ko not_active Expired - Fee Related
- 2014-04-17 WO PCT/IN2014/000247 patent/WO2014170917A1/en not_active Ceased
- 2014-04-17 EP EP14734917.9A patent/EP2986724B1/en not_active Not-in-force
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