CN105358687B - 在有效生产对映体富集的醇中用于酮的对映体选择性还原的设计师细胞及其应用 - Google Patents

在有效生产对映体富集的醇中用于酮的对映体选择性还原的设计师细胞及其应用 Download PDF

Info

Publication number
CN105358687B
CN105358687B CN201480022301.2A CN201480022301A CN105358687B CN 105358687 B CN105358687 B CN 105358687B CN 201480022301 A CN201480022301 A CN 201480022301A CN 105358687 B CN105358687 B CN 105358687B
Authority
CN
China
Prior art keywords
cell
seq
designer
crs
formula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201480022301.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN105358687A (zh
Inventor
高塔姆·斯里瓦斯塔瓦
桑尼特·考尔
拉文德·辛格·乔利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Council of Scientific and Industrial Research CSIR
Original Assignee
Council of Scientific and Industrial Research CSIR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council of Scientific and Industrial Research CSIR filed Critical Council of Scientific and Industrial Research CSIR
Publication of CN105358687A publication Critical patent/CN105358687A/zh
Application granted granted Critical
Publication of CN105358687B publication Critical patent/CN105358687B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/008—Preparation of nitrogen-containing organic compounds containing a N-O bond, e.g. nitro (-NO2), nitroso (-NO)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
CN201480022301.2A 2013-04-17 2014-04-17 在有效生产对映体富集的醇中用于酮的对映体选择性还原的设计师细胞及其应用 Expired - Fee Related CN105358687B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN1146/DEL/2013 2013-04-17
IN1146DE2013 2013-04-17
PCT/IN2014/000247 WO2014170917A1 (en) 2013-04-17 2014-04-17 Designer cells for enantioselective reduction of ketones and use thereof in efficient production of enantioenriched alcohols

Publications (2)

Publication Number Publication Date
CN105358687A CN105358687A (zh) 2016-02-24
CN105358687B true CN105358687B (zh) 2018-10-09

Family

ID=51062858

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480022301.2A Expired - Fee Related CN105358687B (zh) 2013-04-17 2014-04-17 在有效生产对映体富集的醇中用于酮的对映体选择性还原的设计师细胞及其应用

Country Status (6)

Country Link
US (1) US9790524B2 (enExample)
EP (1) EP2986724B1 (enExample)
JP (1) JP6330028B2 (enExample)
KR (1) KR101876172B1 (enExample)
CN (1) CN105358687B (enExample)
WO (1) WO2014170917A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107326049A (zh) * 2016-04-28 2017-11-07 尚科生物医药（上海）有限公司 重组酮还原酶在制备(r)-3,5-二（三氟甲基）苯乙醇中的应用
CN106279374A (zh) * 2016-08-26 2017-01-04 深圳市职业病防治院 一种与铅离子结合的多肽及其应用
CN106399398B (zh) * 2016-11-16 2024-07-09 上海医药工业研究院 (r)-3,5-双(三氟甲基)苯乙醇的生物制备方法
CN107794282B (zh) * 2017-11-20 2020-12-25 浙江工业大学 一种克唑替尼手性中间体的制备方法及菌株
CN112941124B (zh) * 2021-02-09 2023-12-29 江苏阿尔法药业股份有限公司 一种全细胞催化制备依利格鲁司特中间体的方法
CN113789353B (zh) * 2021-09-25 2024-03-08 复旦大学 (2s,3s)-2-氯-3-羟基酯类化合物的制备方法
CN115927138A (zh) * 2022-08-09 2023-04-07 江南大学 一种基于表面展示羰基还原酶的全细胞催化合成2-甲基苯甲酮的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
WO2010151593A1 (en) * 2009-06-23 2010-12-29 Rutgers, The State University Of New Jersey Sterospecific carbonyl reductases

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4710468A (en) 1983-10-24 1987-12-01 Sigma-Tau Industrie Pharmaceutiche Riunite S.P.A. Process for preparing L-carnitine and chemical intermediates employed therein
JPS62126997A (ja) 1985-11-28 1987-06-09 Nippon Synthetic Chem Ind Co Ltd:The 光学活性のγ−ハロ−βヒドロキシ酪酸エステルの製造法
US5413921A (en) 1992-05-28 1995-05-09 Ajinomoto Co., Ltd. Method of the production of (s)-gamma-halogenated-γ-hydroxybutyric acid esters
JP3155107B2 (ja) 1993-01-12 2001-04-09 ダイセル化学工業株式会社 光学活性４−ハロ−３−ヒドロキシ酪酸エステルの製造方法
JPH08336393A (ja) 1995-04-13 1996-12-24 Mitsubishi Chem Corp 光学活性なγ−置換−β−ヒドロキシ酪酸エステルの製造法
US5891685A (en) 1996-06-03 1999-04-06 Mitsubishi Chemical Corporation Method for producing ester of (S)-γ-halogenated-β-hydroxybutyric acid
KR20060064620A (ko) 2003-08-11 2006-06-13 코덱시스, 인코포레이티드 개선된 글루코스 데히드로게나제 폴리펩티드 및 관련폴리뉴클레오티드
KR100743973B1 (ko) 2006-04-27 2007-07-30 한국과학기술원 목적단백질 및 코펙터를 재생시키는 효소를 동시에 세포표면에 표면발현시키는 방법
US20090004720A1 (en) 2007-06-26 2009-01-01 Ling Hua Smart Biocatalysts For Organic Synthesis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005017135A1 (en) * 2003-08-11 2005-02-24 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
WO2010151593A1 (en) * 2009-06-23 2010-12-29 Rutgers, The State University Of New Jersey Sterospecific carbonyl reductases

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Construction and co-expression of a polycistronic plasmid encoding carbonyl reductase and glucose dehydrogenase for production of ethyl (S)-4-chloro-3-hydroxybutanoate;Qi Ye 等;《Bioresource Technology》;20100410;第101卷(第17期);第6767页第3-5行，第6762页第2段第2-3行、图1，第6765页倒数第2段倒数第1-3行 *
Dihydrofolate Reductase (Mouse) and β-Galactosidase (Eescherichia coli) Can Be Translocated across the Plasma Membrane of E.coli;Roland Freudl 等;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;19881231;第263卷(第32期);第17084-17091页 *
不对称还原制备光学纯 (R)-2-羟基-4-苯基丁酸乙酯的双酶共表达重组菌的构建;宿宇宁 等;《催化学报》;20120901;第33卷(第10期);第1650-1660页 *

Also Published As

Publication number Publication date
JP2016515399A (ja) 2016-05-30
KR101876172B1 (ko) 2018-07-10
CN105358687A (zh) 2016-02-24
US9790524B2 (en) 2017-10-17
EP2986724A1 (en) 2016-02-24
EP2986724B1 (en) 2018-10-03
US20160289713A1 (en) 2016-10-06
WO2014170917A1 (en) 2014-10-23
KR20150119347A (ko) 2015-10-23
JP6330028B2 (ja) 2018-05-23

Similar Documents

Publication Publication Date Title
CN105358687B (zh) 在有效生产对映体富集的醇中用于酮的对映体选择性还原的设计师细胞及其应用
US7202069B2 (en) (R)-2-octanol dehydrogenases, methods for producing the enzymes, DNA encoding the enzymes, and methods for producing alcohols using the enzymes
JP2002345479A (ja) 新規な（ｒ）−２，３−ブタンジオール脱水素酵素、その製造方法、及びこれを利用した光学活性アルコールの製造方法
JP2000236883A (ja) 新規なカルボニル還元酵素、該酵素の製造方法、該酵素をコードするｄｎａおよびこれを利用したアルコールの製造方法
Ni et al. Scalable biocatalytic synthesis of optically pure ethyl (R)-2-hydroxy-4-phenylbutyrate using a recombinant E. coli with high catalyst yield
CN105624125B (zh) 醛酮还原酶及其在合成(2s,3r)-2-苯甲酰氨甲基-3-羟基丁酸酯中的应用
CN106701840A (zh) (1r,2s)‑2‑(3,4‑二氟苯基)环丙胺d‑扁桃酸盐（ⅰ）的生物制备方法
WO2016138641A1 (zh) 假丝酵母及其羰基还原酶的产生及应用
US20160281118A1 (en) Method for biotechnological production of methylized cinnamic acids and cinnamic acid esters, methylized phenethylamines and the coupling products thereof, particularly of cinnamic acid amides
EP2562253B1 (en) Modified carbonyl reductase, gene thereof, and method of producing optically active alcohols using these
JP5308163B2 (ja) 新規アルコール脱水素酵素、その遺伝子、ベクター、形質転換体、およびそれらを利用した光学活性アルコールの製造方法
CN113528592B (zh) 酶催化的(2s,3r)-2-取代氨基甲基-3-羟基丁酸酯的合成方法
CN114908129A (zh) 用于制备(r)-4-氯-3-羟基丁酸乙酯的脱氢酶
KR101780510B1 (ko) 케토 화합물의 입체선택적 효소적 환원 방법
JP5789366B2 (ja) 新規なスチレンモノオキシゲナーゼ、その製造方法、およびこれを利用する光学活性なスチレンオキシドの製造方法
JP2005218348A (ja) 光学活性α−ヒドロキシアミドの製造方法
JP2004065049A (ja) Ｄ−マンデル酸脱水素酵素をコードするポリヌクレオチド、及びその用途
EP3649246A1 (en) Method for preparing amines from aldehydes and ketones by biocatalysis
JP2008502334A (ja) 全菌体触媒を用いる光学活性アルコールの製法
WO2005044973A2 (ja) 新規アセトアセチルＣｏＡ還元酵素および光学活性アルコールの製造方法
JP2007274901A (ja) 光学活性プロパルギルアルコールの製造方法
JP2013146236A (ja) 有機溶媒耐性型二級アルコール脱水素酵素
CA2874055A1 (en) Enzymatic reduction
JP2010279272A (ja) 新規カルボニル還元酵素、その遺伝子、ベクター、形質転換体、およびそれらを利用した光学活性アルコールの製造方法
CN106754780A (zh) 一种氧化酶及其应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20181009

CF01 Termination of patent right due to non-payment of annual fee