JP2016504922A5 - - Google Patents
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- JP2016504922A5 JP2016504922A5 JP2015555816A JP2015555816A JP2016504922A5 JP 2016504922 A5 JP2016504922 A5 JP 2016504922A5 JP 2015555816 A JP2015555816 A JP 2015555816A JP 2015555816 A JP2015555816 A JP 2015555816A JP 2016504922 A5 JP2016504922 A5 JP 2016504922A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- mar
- tep
- transgene
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108700019146 Transgenes Proteins 0.000 claims 21
- 150000007523 nucleic acids Chemical group 0.000 claims 20
- 108020004707 nucleic acids Proteins 0.000 claims 17
- 102000039446 nucleic acids Human genes 0.000 claims 17
- 108090000623 proteins and genes Proteins 0.000 claims 16
- 102000004169 proteins and genes Human genes 0.000 claims 15
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 13
- 239000013598 vector Substances 0.000 claims 13
- 210000004962 mammalian cell Anatomy 0.000 claims 9
- -1 Rad54 Proteins 0.000 claims 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 238000005215 recombination Methods 0.000 claims 4
- 230000006798 recombination Effects 0.000 claims 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 241000426682 Salinispora Species 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 3
- 238000011144 upstream manufacturing Methods 0.000 claims 3
- 101150072950 BRCA1 gene Proteins 0.000 claims 2
- 101150059668 Bard1 gene Proteins 0.000 claims 2
- 108091005753 BiP proteins Proteins 0.000 claims 2
- 101150008921 Brca2 gene Proteins 0.000 claims 2
- 102000006311 Cyclin D1 Human genes 0.000 claims 2
- 108010058546 Cyclin D1 Proteins 0.000 claims 2
- 102100033934 DNA repair protein RAD51 homolog 2 Human genes 0.000 claims 2
- 101100300807 Drosophila melanogaster spn-A gene Proteins 0.000 claims 2
- 241000287828 Gallus gallus Species 0.000 claims 2
- 102100027421 Heat shock cognate 71 kDa protein Human genes 0.000 claims 2
- 101001080568 Homo sapiens Heat shock cognate 71 kDa protein Proteins 0.000 claims 2
- 101000581326 Homo sapiens Mediator of DNA damage checkpoint protein 1 Proteins 0.000 claims 2
- 101001048454 Homo sapiens Protein Hook homolog 1 Proteins 0.000 claims 2
- 102000003960 Ligases Human genes 0.000 claims 2
- 108090000364 Ligases Proteins 0.000 claims 2
- 102100027643 Mediator of DNA damage checkpoint protein 1 Human genes 0.000 claims 2
- 108010006519 Molecular Chaperones Proteins 0.000 claims 2
- 102000016943 Muramidase Human genes 0.000 claims 2
- 108010014251 Muramidase Proteins 0.000 claims 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims 2
- 102100023602 Protein Hook homolog 1 Human genes 0.000 claims 2
- 102000053062 Rad52 DNA Repair and Recombination Human genes 0.000 claims 2
- 108700031762 Rad52 DNA Repair and Recombination Proteins 0.000 claims 2
- 108091027967 Small hairpin RNA Proteins 0.000 claims 2
- 102000008579 Transposases Human genes 0.000 claims 2
- 108010020764 Transposases Proteins 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 229960000274 lysozyme Drugs 0.000 claims 2
- 235000010335 lysozyme Nutrition 0.000 claims 2
- 239000004325 lysozyme Substances 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 108091070501 miRNA Proteins 0.000 claims 2
- 239000002679 microRNA Substances 0.000 claims 2
- 101150071637 mre11 gene Proteins 0.000 claims 2
- 230000037361 pathway Effects 0.000 claims 2
- 230000008488 polyadenylation Effects 0.000 claims 2
- 230000022558 protein metabolic process Effects 0.000 claims 2
- 230000020978 protein processing Effects 0.000 claims 2
- 230000009962 secretion pathway Effects 0.000 claims 2
- 239000004055 small Interfering RNA Substances 0.000 claims 2
- 238000001890 transfection Methods 0.000 claims 2
- 241000699800 Cricetinae Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361759802P | 2013-02-01 | 2013-02-01 | |
| US61/759,802 | 2013-02-01 | ||
| US201361800244P | 2013-03-15 | 2013-03-15 | |
| US61/800,244 | 2013-03-15 | ||
| US201361806634P | 2013-03-29 | 2013-03-29 | |
| US61/806,634 | 2013-03-29 | ||
| PCT/IB2014/000100 WO2014118619A2 (en) | 2013-02-01 | 2014-02-01 | Enhanced transgene expression and processing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016504922A JP2016504922A (ja) | 2016-02-18 |
| JP2016504922A5 true JP2016504922A5 (enExample) | 2017-02-16 |
| JP6563816B2 JP6563816B2 (ja) | 2019-08-21 |
Family
ID=50239688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015555816A Active JP6563816B2 (ja) | 2013-02-01 | 2014-02-01 | 増強された導入遺伝子発現およびプロセシング |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US11898154B2 (enExample) |
| EP (2) | EP3536797B1 (enExample) |
| JP (1) | JP6563816B2 (enExample) |
| KR (1) | KR102235603B1 (enExample) |
| CN (2) | CN105073995A (enExample) |
| AU (2) | AU2014210856B2 (enExample) |
| CA (1) | CA2898878C (enExample) |
| DK (2) | DK2951309T3 (enExample) |
| ES (2) | ES2717175T3 (enExample) |
| FI (1) | FI3536797T3 (enExample) |
| HU (2) | HUE043103T2 (enExample) |
| IL (1) | IL240198B (enExample) |
| LT (1) | LT2951309T (enExample) |
| PL (2) | PL3536797T3 (enExample) |
| PT (2) | PT3536797T (enExample) |
| RU (1) | RU2711505C9 (enExample) |
| SG (2) | SG10201803758XA (enExample) |
| SI (1) | SI2951309T1 (enExample) |
| WO (1) | WO2014118619A2 (enExample) |
| ZA (1) | ZA201506419B (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104975009B (zh) * | 2014-04-01 | 2019-10-18 | 三生国健药业（上海）股份有限公司 | 一种新型的含mar核心片段的动物细胞表达载体 |
| CN105154473B (zh) * | 2015-09-30 | 2019-03-01 | 上海细胞治疗研究院 | 一种高效安全的转座子整合系统及其用途 |
| US20180265894A1 (en) * | 2015-10-06 | 2018-09-20 | National University Corporation Okayama University | Gene Expression Cassette And Product Thereof |
| JP6871679B2 (ja) * | 2016-03-23 | 2021-05-12 | 国立大学法人 岡山大学 | 遺伝子発現用カセット及びその産生物 |
| JP2017070224A (ja) * | 2015-10-06 | 2017-04-13 | 国立大学法人 岡山大学 | 遺伝子発現用カセット及びその産生物 |
| WO2017109177A1 (en) | 2015-12-24 | 2017-06-29 | Selexis S.A. | Improved eukaryotic cells for protein manufacturing and methods of making them |
| ITUA20161610A1 (it) * | 2016-03-14 | 2017-09-14 | Angela Anna Messina | Composto peptidico farmacologicamente attivo, procedimento per la sua preparazione e suo uso. |
| EP3219800A1 (en) * | 2016-03-15 | 2017-09-20 | Max-Delbrück-Centrum Für Molekulare Medizin | A transposon-based transfection system for primary cells |
| EP3219803A1 (en) * | 2016-03-15 | 2017-09-20 | Max-Delbrück-Centrum für Molekulare Medizin | Enhanced sleeping beauty transposons, kits and methods of transposition |
| ES2938626T3 (es) * | 2016-05-03 | 2023-04-13 | Lonza Ag | Modulación del metabolismo de los lípidos para la producción de proteínas |
| KR102553990B1 (ko) * | 2016-10-03 | 2023-07-10 | 다이이찌 산쿄 가부시키가이샤 | Hspa5 유전자의 프로모터 |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| KR102523318B1 (ko) | 2016-12-16 | 2023-04-18 | 비-모젠 바이오테크놀로지스, 인크. | 증진된 ｈＡＴ 패밀리 트랜스포존 매개 유전자 전달 및 연관된 조성물, 시스템, 및 방법 |
| WO2018224162A1 (en) * | 2017-06-09 | 2018-12-13 | Biontech Rna Pharmaceuticals Gmbh | Methods for characterizing loss of antigen presentation |
| US12215362B2 (en) * | 2017-09-29 | 2025-02-04 | Bethphagen Inc. | Pharmaceutical composition for prevention or treatment of heart failure |
| CN109929839B (zh) * | 2017-12-18 | 2021-02-12 | 华东师范大学 | 拆分型单碱基基因编辑系统及其应用 |
| CN108504692B (zh) * | 2018-03-26 | 2021-07-23 | 安徽大学 | 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用 |
| EP3794128A1 (en) * | 2018-05-18 | 2021-03-24 | Sorbonne Universite | Molecular tools and methods for transgene integration and their transposition dependent expression |
| JP2021527427A (ja) | 2018-06-21 | 2021-10-14 | ビー−モーゲン・バイオテクノロジーズ，インコーポレーテッド | 強化されたｈＡＴファミリーのトランスポゾンが介在する遺伝子導入ならびに関連する組成物、システム、及び方法 |
| RU2686102C1 (ru) * | 2018-08-07 | 2019-04-24 | Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) | Рекомбинантный вектор для создания плазмидных генетических конструкций, обладающих повышенной длительностью экспрессии целевых генов |
| CA3111045A1 (en) * | 2018-09-05 | 2020-03-12 | Max-Delbruck-Centrum Fur Molekulare Medizin In Der Helmholtz-Gemeinschaft | A method for engineering synthetic cis-regulatory dna |
| WO2020081537A1 (en) * | 2018-10-16 | 2020-04-23 | Texas Tech University System | Method to express, purify, and biotinylate eukaryotic cell-derived major histocompatibility complexes |
| CN113348248B (zh) | 2018-10-24 | 2024-12-31 | 思兰克斯有限公司 | 表达系统、重组细胞及其用途 |
| WO2020123327A1 (en) | 2018-12-10 | 2020-06-18 | Amgen Inc. | Mutated piggybac transposase |
| JP2022516449A (ja) * | 2018-12-24 | 2022-02-28 | セレキシス エスエー | チャイニーズハムスター卵巣細胞における内在性レトロウイルスの特性評価および不活性化 |
| US20220088158A1 (en) | 2019-01-23 | 2022-03-24 | Aceragen, Inc. | Method of ameliorating a pro-inflammatory immunophenotype in farber disease subjects by repeated administration of a recombinant human acid ceramidase |
| IT201900002321A1 (it) * | 2019-02-18 | 2020-08-18 | Univ Degli Studi Padova | Peptidi con attivita' antitumorale |
| TWI818166B (zh) * | 2019-03-27 | 2023-10-11 | 美商菲尼克斯組織修復公司 | 用於生產膠原蛋白7組合物之系統及方法 |
| CN110006866B (zh) * | 2019-04-16 | 2022-04-22 | 杭州迈尔德生物科技有限公司 | 一种阿片类活性物质的通用检测方法及其检测试剂盒 |
| WO2020228721A1 (en) * | 2019-05-13 | 2020-11-19 | Lin Mei Chun | Method and kit for monitoring cancer |
| CA3154452A1 (en) * | 2019-10-10 | 2021-04-15 | The Regents Of The University Of California | Methods to stabilize mammalian cells |
| IL270306A (en) | 2019-10-30 | 2021-05-31 | Yeda Res & Dev | Prevention and treatment of pre-myeloid and myeloid malignancies |
| CN112813099A (zh) * | 2019-11-15 | 2021-05-18 | 上海细胞治疗集团有限公司 | 一种在活化的t细胞中具有高活性的启动子 |
| CN110863008B (zh) * | 2019-11-22 | 2020-10-16 | 昆明学院 | Mar序列调控弱启动子构建高效表达载体的方法 |
| CA3164931A1 (en) | 2019-12-24 | 2021-07-01 | Selexis Sa | Targeted integration in mammalian sequences enhancing gene expression |
| US20230203530A1 (en) * | 2020-02-19 | 2023-06-29 | WuXi Biologics Ireland Limited | Enhanced expression system and methods of use thereof |
| CN111500629B (zh) * | 2020-04-17 | 2021-11-19 | 安徽中盛溯源生物科技有限公司 | 一种高表达层粘连蛋白-511变体的方法及其应用 |
| EP4149966A4 (en) * | 2020-05-14 | 2024-08-07 | Seattle Children's Hospital (DBA Seattle Children's Research Institute) | DOUBLE PROMOTOR SYSTEMS |
| US12173304B2 (en) * | 2020-07-08 | 2024-12-24 | Neuracle Genetics Inc. | Intron fragments |
| CN113881703B (zh) * | 2021-10-11 | 2022-06-21 | 中国人民解放军军事科学院军事医学研究院 | 一种提高cho细胞同源重组效率的方法及其相关产品和应用 |
| CN116440269B (zh) * | 2022-01-10 | 2024-02-09 | 华中农业大学 | Slc35a1基因作为靶点在防治猪流行性腹泻病中的应用 |
| CN114958914B (zh) * | 2022-06-06 | 2023-09-26 | 新乡医学院 | 一种人类及哺乳动物细胞附着体表达载体、构建方法和应用 |
| CN115896227A (zh) * | 2022-12-20 | 2023-04-04 | 西北农林科技大学 | 一种鉴定外源基因整合位点以增强转基因表达的方法 |
| GB202305637D0 (en) | 2023-04-17 | 2023-05-31 | Macomics Ltd | Transposons, vectors and genetically engineered cells |
| GB202415693D0 (en) | 2024-10-24 | 2024-12-11 | Macomics Ltd | Genetically-engineered cells |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5731178A (en) | 1990-03-21 | 1998-03-24 | Behringwerke Aktiengesellschaft | Attachment-elements for stimulation of eukaryotic expression systems |
| JP3656277B2 (ja) * | 1995-05-17 | 2005-06-08 | 味の素株式会社 | 組換えｄｎａ法によるトランスグルタミナーゼの効率的製造法 |
| EP1395669B1 (en) | 2001-01-26 | 2009-07-22 | Selexis S.A. | Matrix attachment regions and methods for use thereof |
| US20040235011A1 (en) * | 2002-06-26 | 2004-11-25 | Cooper Richard K. | Production of multimeric proteins |
| US20040203158A1 (en) * | 2003-01-15 | 2004-10-14 | Hackett Perry B. | Transposon-insulator element delivery systems |
| US7244616B2 (en) | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| PL1959011T3 (pl) | 2003-10-24 | 2012-07-31 | Selexis Sa | Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy |
| ES2292271B1 (es) * | 2004-05-20 | 2009-02-16 | Proyecto De Biomedicina Cima, S.L. | Un vector hibrido adenovirus-alfavirus para la administracion eficaz y expresion de genes terapeuticos en celulas tumorales. |
| JP2008543283A (ja) * | 2005-06-10 | 2008-12-04 | ジェネンテック・インコーポレーテッド | インスレーターエレメントを含むベクターを使用する抗体発現の改良 |
| EP3156414B1 (en) | 2007-09-26 | 2019-12-04 | Intrexon Corporation | Synthetic 5'utrs, expression vectors, and methods for increasing transgene expression |
| US20090247609A1 (en) | 2007-12-20 | 2009-10-01 | Hitto Kaufmann | Sm-protein based secretion engineering |
| US20100105140A1 (en) * | 2008-07-16 | 2010-04-29 | Fahrenkrug Scott C | Plaice dna transposon system |
| WO2010118360A1 (en) * | 2009-04-09 | 2010-10-14 | The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Production of proteins using transposon-based vectors |
| CN102575264A (zh) * | 2009-09-18 | 2012-07-11 | 瑟莱克斯公司 | 增加的转基因表达和加工的产品和方法 |
-
2014
- 2014-02-01 HU HUE14708935A patent/HUE043103T2/hu unknown
- 2014-02-01 DK DK14708935.3T patent/DK2951309T3/en active
- 2014-02-01 EP EP19153376.9A patent/EP3536797B1/en active Active
- 2014-02-01 PL PL19153376.9T patent/PL3536797T3/pl unknown
- 2014-02-01 SI SI201431115T patent/SI2951309T1/sl unknown
- 2014-02-01 ES ES14708935T patent/ES2717175T3/es active Active
- 2014-02-01 DK DK19153376.9T patent/DK3536797T5/da active
- 2014-02-01 KR KR1020157022393A patent/KR102235603B1/ko active Active
- 2014-02-01 EP EP14708935.3A patent/EP2951309B1/en active Active
- 2014-02-01 FI FIEP19153376.9T patent/FI3536797T3/fi active
- 2014-02-01 US US14/761,460 patent/US11898154B2/en active Active
- 2014-02-01 SG SG10201803758XA patent/SG10201803758XA/en unknown
- 2014-02-01 JP JP2015555816A patent/JP6563816B2/ja active Active
- 2014-02-01 CN CN201480010305.9A patent/CN105073995A/zh active Pending
- 2014-02-01 RU RU2015127667A patent/RU2711505C9/ru active
- 2014-02-01 PT PT191533769T patent/PT3536797T/pt unknown
- 2014-02-01 HU HUE19153376A patent/HUE061494T2/hu unknown
- 2014-02-01 PT PT14708935T patent/PT2951309T/pt unknown
- 2014-02-01 PL PL14708935T patent/PL2951309T3/pl unknown
- 2014-02-01 LT LTEP14708935.3T patent/LT2951309T/lt unknown
- 2014-02-01 AU AU2014210856A patent/AU2014210856B2/en active Active
- 2014-02-01 CN CN202510831703.7A patent/CN120718957A/zh active Pending
- 2014-02-01 ES ES19153376T patent/ES2940433T3/es active Active
- 2014-02-01 CA CA2898878A patent/CA2898878C/en active Active
- 2014-02-01 WO PCT/IB2014/000100 patent/WO2014118619A2/en not_active Ceased
- 2014-02-01 SG SG11201505487UA patent/SG11201505487UA/en unknown
-
2015
- 2015-07-28 IL IL24019815A patent/IL240198B/en active IP Right Grant
- 2015-09-01 ZA ZA2015/06419A patent/ZA201506419B/en unknown
-
2019
- 2019-10-18 AU AU2019250224A patent/AU2019250224B2/en active Active
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