JP2016504922A5 - - Google Patents
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- JP2016504922A5 JP2016504922A5 JP2015555816A JP2015555816A JP2016504922A5 JP 2016504922 A5 JP2016504922 A5 JP 2016504922A5 JP 2015555816 A JP2015555816 A JP 2015555816A JP 2015555816 A JP2015555816 A JP 2015555816A JP 2016504922 A5 JP2016504922 A5 JP 2016504922A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- mar
- tep
- transgene
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108700019146 Transgenes Proteins 0.000 claims 21
- 150000007523 nucleic acids Chemical group 0.000 claims 20
- 108020004707 nucleic acids Proteins 0.000 claims 17
- 102000039446 nucleic acids Human genes 0.000 claims 17
- 108090000623 proteins and genes Proteins 0.000 claims 16
- 102000004169 proteins and genes Human genes 0.000 claims 15
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 13
- 239000013598 vector Substances 0.000 claims 13
- 210000004962 mammalian cell Anatomy 0.000 claims 9
- -1 Rad54 Proteins 0.000 claims 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 238000000034 method Methods 0.000 claims 4
- 238000005215 recombination Methods 0.000 claims 4
- 230000006798 recombination Effects 0.000 claims 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 241000426682 Salinispora Species 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 3
- 238000011144 upstream manufacturing Methods 0.000 claims 3
- 101150072950 BRCA1 gene Proteins 0.000 claims 2
- 101150059668 Bard1 gene Proteins 0.000 claims 2
- 108091005753 BiP proteins Proteins 0.000 claims 2
- 101150008921 Brca2 gene Proteins 0.000 claims 2
- 102000006311 Cyclin D1 Human genes 0.000 claims 2
- 108010058546 Cyclin D1 Proteins 0.000 claims 2
- 102100033934 DNA repair protein RAD51 homolog 2 Human genes 0.000 claims 2
- 101100300807 Drosophila melanogaster spn-A gene Proteins 0.000 claims 2
- 241000287828 Gallus gallus Species 0.000 claims 2
- 102100027421 Heat shock cognate 71 kDa protein Human genes 0.000 claims 2
- 101001080568 Homo sapiens Heat shock cognate 71 kDa protein Proteins 0.000 claims 2
- 101000581326 Homo sapiens Mediator of DNA damage checkpoint protein 1 Proteins 0.000 claims 2
- 101001048454 Homo sapiens Protein Hook homolog 1 Proteins 0.000 claims 2
- 102000003960 Ligases Human genes 0.000 claims 2
- 108090000364 Ligases Proteins 0.000 claims 2
- 102100027643 Mediator of DNA damage checkpoint protein 1 Human genes 0.000 claims 2
- 108010006519 Molecular Chaperones Proteins 0.000 claims 2
- 102000016943 Muramidase Human genes 0.000 claims 2
- 108010014251 Muramidase Proteins 0.000 claims 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims 2
- 102100023602 Protein Hook homolog 1 Human genes 0.000 claims 2
- 102000053062 Rad52 DNA Repair and Recombination Human genes 0.000 claims 2
- 108700031762 Rad52 DNA Repair and Recombination Proteins 0.000 claims 2
- 108091027967 Small hairpin RNA Proteins 0.000 claims 2
- 102000008579 Transposases Human genes 0.000 claims 2
- 108010020764 Transposases Proteins 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 229960000274 lysozyme Drugs 0.000 claims 2
- 235000010335 lysozyme Nutrition 0.000 claims 2
- 239000004325 lysozyme Substances 0.000 claims 2
- 108020004999 messenger RNA Proteins 0.000 claims 2
- 108091070501 miRNA Proteins 0.000 claims 2
- 239000002679 microRNA Substances 0.000 claims 2
- 101150071637 mre11 gene Proteins 0.000 claims 2
- 230000037361 pathway Effects 0.000 claims 2
- 230000008488 polyadenylation Effects 0.000 claims 2
- 230000022558 protein metabolic process Effects 0.000 claims 2
- 230000020978 protein processing Effects 0.000 claims 2
- 230000009962 secretion pathway Effects 0.000 claims 2
- 239000004055 small Interfering RNA Substances 0.000 claims 2
- 238000001890 transfection Methods 0.000 claims 2
- 241000699800 Cricetinae Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361759802P | 2013-02-01 | 2013-02-01 | |
| US61/759,802 | 2013-02-01 | ||
| US201361800244P | 2013-03-15 | 2013-03-15 | |
| US61/800,244 | 2013-03-15 | ||
| US201361806634P | 2013-03-29 | 2013-03-29 | |
| US61/806,634 | 2013-03-29 | ||
| PCT/IB2014/000100 WO2014118619A2 (en) | 2013-02-01 | 2014-02-01 | Enhanced transgene expression and processing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016504922A JP2016504922A (ja) | 2016-02-18 |
| JP2016504922A5 true JP2016504922A5 (enExample) | 2017-02-16 |
| JP6563816B2 JP6563816B2 (ja) | 2019-08-21 |
Family
ID=50239688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015555816A Active JP6563816B2 (ja) | 2013-02-01 | 2014-02-01 | 増強された導入遺伝子発現およびプロセシング |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US11898154B2 (enExample) |
| EP (2) | EP2951309B1 (enExample) |
| JP (1) | JP6563816B2 (enExample) |
| KR (1) | KR102235603B1 (enExample) |
| CN (2) | CN120718957A (enExample) |
| AU (2) | AU2014210856B2 (enExample) |
| CA (1) | CA2898878C (enExample) |
| DK (2) | DK2951309T3 (enExample) |
| ES (2) | ES2717175T3 (enExample) |
| FI (1) | FI3536797T3 (enExample) |
| HU (2) | HUE061494T2 (enExample) |
| IL (1) | IL240198B (enExample) |
| LT (1) | LT2951309T (enExample) |
| PL (2) | PL3536797T3 (enExample) |
| PT (2) | PT2951309T (enExample) |
| RU (1) | RU2711505C9 (enExample) |
| SG (2) | SG10201803758XA (enExample) |
| SI (1) | SI2951309T1 (enExample) |
| WO (1) | WO2014118619A2 (enExample) |
| ZA (1) | ZA201506419B (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109576269B (zh) * | 2014-04-01 | 2020-08-28 | 三生国健药业（上海）股份有限公司 | 一种含mar核心片段的动物细胞表达载体 |
| CN105154473B (zh) * | 2015-09-30 | 2019-03-01 | 上海细胞治疗研究院 | 一种高效安全的转座子整合系统及其用途 |
| US20180265894A1 (en) * | 2015-10-06 | 2018-09-20 | National University Corporation Okayama University | Gene Expression Cassette And Product Thereof |
| JP6871679B2 (ja) * | 2016-03-23 | 2021-05-12 | 国立大学法人 岡山大学 | 遺伝子発現用カセット及びその産生物 |
| JP2017070224A (ja) * | 2015-10-06 | 2017-04-13 | 国立大学法人 岡山大学 | 遺伝子発現用カセット及びその産生物 |
| WO2017109177A1 (en) | 2015-12-24 | 2017-06-29 | Selexis S.A. | Improved eukaryotic cells for protein manufacturing and methods of making them |
| ITUA20161610A1 (it) * | 2016-03-14 | 2017-09-14 | Angela Anna Messina | Composto peptidico farmacologicamente attivo, procedimento per la sua preparazione e suo uso. |
| EP3219800A1 (en) * | 2016-03-15 | 2017-09-20 | Max-Delbrück-Centrum Für Molekulare Medizin | A transposon-based transfection system for primary cells |
| EP3219803A1 (en) * | 2016-03-15 | 2017-09-20 | Max-Delbrück-Centrum für Molekulare Medizin | Enhanced sleeping beauty transposons, kits and methods of transposition |
| WO2017191165A1 (en) * | 2016-05-03 | 2017-11-09 | Lonza Ltd | Modulation of lipid metabolism for protein production |
| JP7382138B2 (ja) * | 2016-10-03 | 2023-11-16 | 第一三共株式会社 | Ｈｓｐａ５遺伝子のプロモーター |
| CA3047313A1 (en) | 2016-12-16 | 2018-06-21 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| WO2018224162A1 (en) * | 2017-06-09 | 2018-12-13 | Biontech Rna Pharmaceuticals Gmbh | Methods for characterizing loss of antigen presentation |
| JP7240749B2 (ja) * | 2017-09-29 | 2023-03-16 | ベスファジェン・インコーポレイテッド | 心不全の予防または治療のための医薬組成物 |
| CN109929839B (zh) * | 2017-12-18 | 2021-02-12 | 华东师范大学 | 拆分型单碱基基因编辑系统及其应用 |
| CN108504692B (zh) * | 2018-03-26 | 2021-07-23 | 安徽大学 | 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用 |
| WO2019219947A1 (en) * | 2018-05-18 | 2019-11-21 | Sorbonne Universite | Molecular tools and methods for transgene integration and their transposition dependent expression |
| WO2019246486A2 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
| RU2686102C1 (ru) * | 2018-08-07 | 2019-04-24 | Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) | Рекомбинантный вектор для создания плазмидных генетических конструкций, обладающих повышенной длительностью экспрессии целевых генов |
| US20210343368A1 (en) * | 2018-09-05 | 2021-11-04 | Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft | Method for Engineering Synthetic Cis-Regulatory DNA |
| WO2020081537A1 (en) * | 2018-10-16 | 2020-04-23 | Texas Tech University System | Method to express, purify, and biotinylate eukaryotic cell-derived major histocompatibility complexes |
| US20230193341A1 (en) | 2018-10-24 | 2023-06-22 | Selexis Sa | Expression systems, recombinant cells and uses thereof |
| HUE068630T2 (hu) | 2018-12-10 | 2025-01-28 | Amgen Inc | Mutáns PiggyBac transzpozáz |
| US12404527B2 (en) * | 2018-12-24 | 2025-09-02 | Selexs S.A. | Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells |
| US20220088158A1 (en) | 2019-01-23 | 2022-03-24 | Aceragen, Inc. | Method of ameliorating a pro-inflammatory immunophenotype in farber disease subjects by repeated administration of a recombinant human acid ceramidase |
| IT201900002321A1 (it) * | 2019-02-18 | 2020-08-18 | Univ Degli Studi Padova | Peptidi con attivita' antitumorale |
| AU2020248453A1 (en) * | 2019-03-27 | 2021-10-28 | Phoenix Tissue Repair, Inc. | Systems and methods for producing collagen 7 compositions |
| CN110006866B (zh) * | 2019-04-16 | 2022-04-22 | 杭州迈尔德生物科技有限公司 | 一种阿片类活性物质的通用检测方法及其检测试剂盒 |
| WO2020228721A1 (en) * | 2019-05-13 | 2020-11-19 | Lin Mei Chun | Method and kit for monitoring cancer |
| EP4041895A1 (en) * | 2019-10-10 | 2022-08-17 | The Regents of the University of California | Methods to stabilize mammalian cells |
| IL270306A (en) | 2019-10-30 | 2021-05-31 | Yeda Res & Dev | Prevention and treatment of pre-myeloid and myeloid malignancies |
| CN112813099A (zh) * | 2019-11-15 | 2021-05-18 | 上海细胞治疗集团有限公司 | 一种在活化的t细胞中具有高活性的启动子 |
| CN110863008B (zh) * | 2019-11-22 | 2020-10-16 | 昆明学院 | Mar序列调控弱启动子构建高效表达载体的方法 |
| US20230046668A1 (en) | 2019-12-24 | 2023-02-16 | Selexis S.A. | Targeted integration in mammalian sequences enhancing gene expression |
| KR102937659B1 (ko) * | 2020-02-19 | 2026-03-10 | 우시 바이올로직스 아일랜드 리미티드 | 강화된 발현 시스템 및 그 사용방법 |
| CN111500629B (zh) * | 2020-04-17 | 2021-11-19 | 安徽中盛溯源生物科技有限公司 | 一种高表达层粘连蛋白-511变体的方法及其应用 |
| US20230242936A1 (en) * | 2020-05-14 | 2023-08-03 | Seattle Children's Hospital (dba Seattle Children's Research Institute) | Dual promoter systems |
| US12173304B2 (en) * | 2020-07-08 | 2024-12-24 | Neuracle Genetics Inc. | Intron fragments |
| CN113881703B (zh) * | 2021-10-11 | 2022-06-21 | 中国人民解放军军事科学院军事医学研究院 | 一种提高cho细胞同源重组效率的方法及其相关产品和应用 |
| CN116440269B (zh) * | 2022-01-10 | 2024-02-09 | 华中农业大学 | Slc35a1基因作为靶点在防治猪流行性腹泻病中的应用 |
| CN114958914B (zh) * | 2022-06-06 | 2023-09-26 | 新乡医学院 | 一种人类及哺乳动物细胞附着体表达载体、构建方法和应用 |
| CN115896227A (zh) * | 2022-12-20 | 2023-04-04 | 西北农林科技大学 | 一种鉴定外源基因整合位点以增强转基因表达的方法 |
| GB202305637D0 (en) | 2023-04-17 | 2023-05-31 | Macomics Ltd | Transposons, vectors and genetically engineered cells |
| GB202415693D0 (en) | 2024-10-24 | 2024-12-11 | Macomics Ltd | Genetically-engineered cells |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5731178A (en) | 1990-03-21 | 1998-03-24 | Behringwerke Aktiengesellschaft | Attachment-elements for stimulation of eukaryotic expression systems |
| JP3656277B2 (ja) * | 1995-05-17 | 2005-06-08 | 味の素株式会社 | 組換えｄｎａ法によるトランスグルタミナーゼの効率的製造法 |
| DK1395669T3 (da) | 2001-01-26 | 2009-11-16 | Selexis Sa | Matriks bindingsregioner og fremgangsmåder til anvendelse af disse |
| US20040235011A1 (en) * | 2002-06-26 | 2004-11-25 | Cooper Richard K. | Production of multimeric proteins |
| WO2004065581A2 (en) * | 2003-01-15 | 2004-08-05 | Discovery Genomics, Inc. | Transposon-insulator element delivery systems |
| US7244616B2 (en) * | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| KR101269656B1 (ko) | 2003-10-24 | 2013-05-30 | 셀렉시스 에스. 에이. | Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| ES2292271B1 (es) * | 2004-05-20 | 2009-02-16 | Proyecto De Biomedicina Cima, S.L. | Un vector hibrido adenovirus-alfavirus para la administracion eficaz y expresion de genes terapeuticos en celulas tumorales. |
| WO2007021353A2 (en) * | 2005-06-10 | 2007-02-22 | Genentech, Inc. | Improving antibody expression using vectors containing insulator elements |
| DK2205618T3 (en) | 2007-09-26 | 2017-02-20 | Intrexon Corp | SYNTHETIC 5 'NON-TRANSLATED REGIONS, EXPRESSION VECTORS AND PROCEDURES FOR INCREASING TRANSGENIC EXPRESSION |
| US20090247609A1 (en) | 2007-12-20 | 2009-10-01 | Hitto Kaufmann | Sm-protein based secretion engineering |
| WO2010008564A2 (en) * | 2008-07-16 | 2010-01-21 | Recombinetics | Plaice dna transposon system |
| EP2417263B1 (en) * | 2009-04-09 | 2015-09-23 | ProteoVec Holding L.L.C. | Production of proteins using transposon-based vectors |
| CN102575264A (zh) * | 2009-09-18 | 2012-07-11 | 瑟莱克斯公司 | 增加的转基因表达和加工的产品和方法 |
-
2014
- 2014-02-01 DK DK14708935.3T patent/DK2951309T3/en active
- 2014-02-01 US US14/761,460 patent/US11898154B2/en active Active
- 2014-02-01 KR KR1020157022393A patent/KR102235603B1/ko active Active
- 2014-02-01 SI SI201431115T patent/SI2951309T1/sl unknown
- 2014-02-01 CN CN202510831703.7A patent/CN120718957A/zh active Pending
- 2014-02-01 WO PCT/IB2014/000100 patent/WO2014118619A2/en not_active Ceased
- 2014-02-01 PL PL19153376.9T patent/PL3536797T3/pl unknown
- 2014-02-01 RU RU2015127667A patent/RU2711505C9/ru active
- 2014-02-01 DK DK19153376.9T patent/DK3536797T5/da active
- 2014-02-01 PT PT14708935T patent/PT2951309T/pt unknown
- 2014-02-01 HU HUE19153376A patent/HUE061494T2/hu unknown
- 2014-02-01 PL PL14708935T patent/PL2951309T3/pl unknown
- 2014-02-01 ES ES14708935T patent/ES2717175T3/es active Active
- 2014-02-01 CA CA2898878A patent/CA2898878C/en active Active
- 2014-02-01 CN CN201480010305.9A patent/CN105073995A/zh active Pending
- 2014-02-01 JP JP2015555816A patent/JP6563816B2/ja active Active
- 2014-02-01 LT LTEP14708935.3T patent/LT2951309T/lt unknown
- 2014-02-01 PT PT191533769T patent/PT3536797T/pt unknown
- 2014-02-01 SG SG10201803758XA patent/SG10201803758XA/en unknown
- 2014-02-01 EP EP14708935.3A patent/EP2951309B1/en active Active
- 2014-02-01 ES ES19153376T patent/ES2940433T3/es active Active
- 2014-02-01 HU HUE14708935A patent/HUE043103T2/hu unknown
- 2014-02-01 FI FIEP19153376.9T patent/FI3536797T3/fi active
- 2014-02-01 SG SG11201505487UA patent/SG11201505487UA/en unknown
- 2014-02-01 AU AU2014210856A patent/AU2014210856B2/en active Active
- 2014-02-01 EP EP19153376.9A patent/EP3536797B1/en active Active
-
2015
- 2015-07-28 IL IL24019815A patent/IL240198B/en active IP Right Grant
- 2015-09-01 ZA ZA2015/06419A patent/ZA201506419B/en unknown
-
2019
- 2019-10-18 AU AU2019250224A patent/AU2019250224B2/en active Active
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