JP2015526090A - グルコアミラーゼ活性を有する変異体 - Google Patents

グルコアミラーゼ活性を有する変異体 Download PDF

Info

Publication number
JP2015526090A
JP2015526090A JP2015527905A JP2015527905A JP2015526090A JP 2015526090 A JP2015526090 A JP 2015526090A JP 2015527905 A JP2015527905 A JP 2015527905A JP 2015527905 A JP2015527905 A JP 2015527905A JP 2015526090 A JP2015526090 A JP 2015526090A
Authority
JP
Japan
Prior art keywords
glucoamylase
seq
amino acid
parent
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2015527905A
Other languages
English (en)
Japanese (ja)
Inventor
ヤコブ・フリフホルム・クラマー
ペータ・エドヴァルド・ダイン
イゴール・ニコラエフ
ヴィクトル・アレクセーエフ
キャスパー・ウィレム・ヴローメン
パズミノ ダニエル・トーレス
パズミノ ダニエル・トーレス
ネヴィル・マーシャル・フィッシュ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International N&H Denmark ApS
Original Assignee
DuPont Nutrition Biosciences ApS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DuPont Nutrition Biosciences ApS filed Critical DuPont Nutrition Biosciences ApS
Publication of JP2015526090A publication Critical patent/JP2015526090A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E10/00—Energy generation through renewable energy sources
    • Y02E10/30—Energy from the sea, e.g. using wave energy or salinity gradient

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015527905A 2012-08-22 2013-08-21 グルコアミラーゼ活性を有する変異体 Pending JP2015526090A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261692040P 2012-08-22 2012-08-22
US61/692,040 2012-08-22
EP12189588 2012-10-23
EP12189588.2 2012-10-23
PCT/EP2013/067391 WO2014029808A1 (en) 2012-08-22 2013-08-21 Variants having glucoamylase activity

Publications (1)

Publication Number Publication Date
JP2015526090A true JP2015526090A (ja) 2015-09-10

Family

ID=47046486

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015527905A Pending JP2015526090A (ja) 2012-08-22 2013-08-21 グルコアミラーゼ活性を有する変異体

Country Status (9)

Country Link
US (2) US20150315558A1 (enExample)
EP (1) EP2888357A1 (enExample)
JP (1) JP2015526090A (enExample)
CN (1) CN104619836A (enExample)
AU (1) AU2013305007A1 (enExample)
EA (1) EA201590425A1 (enExample)
IN (1) IN2015DN00231A (enExample)
MX (1) MX2015002099A (enExample)
WO (1) WO2014029808A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017195801A (ja) * 2016-04-26 2017-11-02 サッポロビール株式会社 ビールテイスト飲料の製造方法及びビールテイスト飲料
JP2019062786A (ja) * 2017-09-29 2019-04-25 群栄化学工業株式会社 麦汁もしくは麦芽エキス、又は醸造酒の製造方法
JP2020137526A (ja) * 2020-06-12 2020-09-03 サッポロビール株式会社 ビールテイスト飲料

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105960176A (zh) * 2014-02-07 2016-09-21 诺维信公司 用于生产葡萄糖糖浆的组合物
US20180002683A1 (en) 2014-12-18 2018-01-04 Danisco Us Inc. Engineered multifunctional enzymes and methods of use
EP3234119A1 (en) 2014-12-18 2017-10-25 Danisco US Inc. Engineered multifunctional enzymes and methods of use
BR112017016821A2 (pt) 2015-02-06 2018-04-03 Cargill Inc polipeptídeo, ácido nucleico, vetor, célula hospedeira, meio de fermentação, uso de polipeptídeo, composição de ração e método de fermentação
US10344288B2 (en) 2015-03-27 2019-07-09 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
CA3007420A1 (en) 2015-12-17 2017-06-22 Cargill, Incorporated Sugar transporter-modified yeast strains and methods for bioproduct production
CA3032736A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
US11634673B2 (en) 2017-09-29 2023-04-25 Dupont Nutrition Biosciences Aps Production of brewer's wort having increase fermentable sugars for fermentation
MX2020009344A (es) 2018-03-09 2020-11-13 Danisco Us Inc Glucoamilasas y métodos para su uso.
WO2019186371A1 (en) * 2018-03-26 2019-10-03 Lallemand Hungary Liquidity Management Llc Chimeric amylases comprising an heterologous starch binding domain
CN109266633A (zh) * 2018-09-14 2019-01-25 华东理工大学 碳水化合物结合模块及其在固定化酶制备与融合蛋白纯化上的应用
US20230167386A1 (en) * 2020-05-21 2023-06-01 Dupont Nutrition Biosciences Aps Uninhibited amylases for brewing with high tannin materials
CN112662648B (zh) * 2021-01-04 2022-09-06 杭州荔枝红生物科技有限公司 一种木聚糖酶GH11-xylanase突变体及其应用
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
GB2089836B (en) 1980-12-16 1984-06-20 Suntory Ltd Process for producing alcohol by fermentation without cooking
DK252483D0 (da) 1983-06-03 1983-06-03 Novo Industri As Alpha-acetolactate decarboxylaseemzymprodukt og fremstilling deraf
US4794175A (en) 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
US4666718A (en) 1984-08-15 1987-05-19 Miller Brewing Company Preparation of low calorie beer
US4618579A (en) 1984-09-28 1986-10-21 Genencor, Inc. Raw starch saccharification
JPH0630586B2 (ja) 1984-12-15 1994-04-27 サントリー株式会社 グルコアミラ−ゼ遺伝子
DE3650202T3 (de) 1985-08-29 2004-06-03 Genencor International, Inc., South San Francisco Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung.
US5024941A (en) 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
EP0316444B1 (en) 1987-06-06 1995-08-02 Omnigene Inc Yeast expression vector
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
JPH06502226A (ja) 1990-10-05 1994-03-10 ジェネンコア　インターナショナル　インコーポレーテッド セルラーゼによる綿含有織物の処理方法
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
EP1225227B1 (en) 1990-12-10 2009-02-18 Genencor International, Inc. Improved saccharification of cellulose by cloning and amplification of the beta-glucosidase gene of trichoderma reesei
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
EP1559776A3 (en) 1994-06-30 2006-01-11 Novozymes Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system and promoters and terminators for use therein
US5744716A (en) 1995-06-08 1998-04-28 Scp Global Technologies, A Division Of Preco, Inc. Fluid displacement level, density and concentration measurement system
US6255084B1 (en) 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
US6352851B1 (en) * 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6254914B1 (en) 1999-07-02 2001-07-03 The Board Of Trustees Of The University Of Illinois Process for recovery of corn coarse fiber (pericarp)
CA2418317A1 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
GB0129864D0 (en) 2001-12-13 2002-02-06 Danisco Animal feed
ES2398216T3 (es) 2003-03-10 2013-03-14 Poet Research, Inc. Método para producir etanol usando almidón sin procesar
US20040219649A1 (en) 2003-03-10 2004-11-04 Novozymes A/S Alcohol product processes
ATE542895T1 (de) 2003-05-29 2012-02-15 Danisco Us Inc Neue trichoderma-gene
US6899910B2 (en) 2003-06-12 2005-05-31 The United States Of America As Represented By The Secretary Of Agriculture Processes for recovery of corn germ and optionally corn coarse fiber (pericarp)
CN102174490B (zh) 2003-11-21 2016-09-28 丹尼斯科美国公司 颗粒淀粉水解酶在木霉菌中的表达和从颗粒淀粉底物产生葡萄糖的方法
KR101222413B1 (ko) 2004-05-27 2013-01-16 다니스코 유에스 인크. 아스퍼질러스 가와치 산-안정성 알파 아밀라제의 이종 발현및 입상 전분 가수분해에서의 이용
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
DK1824970T3 (da) 2004-11-30 2011-10-31 Genencor Int Thricoderma reesei-glycoamylase og homologer heraf
JP5087407B2 (ja) 2004-12-30 2012-12-05 ジェネンコー・インターナショナル・インク 酸性真菌プロテアーゼ
DK2402425T3 (da) 2006-07-13 2021-07-05 Dsm Ip Assets Bv Forbedret brygningsproces
JP5568307B2 (ja) 2006-10-10 2014-08-06 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン 修飾された性質を有するグルコアミラーゼ変異体
DE102007032649A1 (de) 2007-07-13 2009-01-15 Man Nutzfahrzeuge Ag Wanne und Verfahren zu ihrer Herstellung
ES2504983T3 (es) 2007-10-09 2014-10-09 Danisco Us Inc. Variantes de glucoamilasa
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
DK3323889T3 (da) * 2007-11-20 2020-06-29 Danisco Us Inc Glucoamylasevarianter med ændrede egenskaber
EP2438164B1 (en) 2009-06-03 2017-08-02 Danisco US Inc. Cellulase variants with improved expression, activity and/or stability, and use thereof
US20120164695A1 (en) * 2009-08-19 2012-06-28 Danisco Us Inc. Combinatorial variants of glucoamylase with improved specific activity and/or thermostability
EP2467474A1 (en) 2009-08-19 2012-06-27 Danisco A/S Variants of glucoamylase

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017195801A (ja) * 2016-04-26 2017-11-02 サッポロビール株式会社 ビールテイスト飲料の製造方法及びビールテイスト飲料
WO2017187695A1 (ja) * 2016-04-26 2017-11-02 サッポロホールディングス株式会社 ビールテイスト飲料の製造方法及びビールテイスト飲料
JP2019062786A (ja) * 2017-09-29 2019-04-25 群栄化学工業株式会社 麦汁もしくは麦芽エキス、又は醸造酒の製造方法
JP7115830B2 (ja) 2017-09-29 2022-08-09 群栄化学工業株式会社 麦汁もしくは麦芽エキス、又は醸造酒の製造方法
JP2020137526A (ja) * 2020-06-12 2020-09-03 サッポロビール株式会社 ビールテイスト飲料
JP7096290B2 (ja) 2020-06-12 2022-07-05 サッポロビール株式会社 ビールテイスト飲料

Also Published As

Publication number Publication date
US20150315558A1 (en) 2015-11-05
EP2888357A1 (en) 2015-07-01
US20170306309A1 (en) 2017-10-26
EA201590425A1 (ru) 2015-06-30
MX2015002099A (es) 2015-05-11
CN104619836A (zh) 2015-05-13
IN2015DN00231A (enExample) 2015-06-12
AU2013305007A1 (en) 2015-01-29
WO2014029808A1 (en) 2014-02-27

Similar Documents

Publication Publication Date Title
JP2015526090A (ja) グルコアミラーゼ活性を有する変異体
JP5827227B2 (ja) グルコアミラーゼの変異体
JP5594899B2 (ja) 変更された性質を有するグルコアミラーゼ変異体
JP5594898B2 (ja) 性質が変えられたグルコアミラーゼ変異種
US9677058B2 (en) Polypeptides having glucoamylase activity and method of producing the same
CN102712915B (zh) 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体
CN113403357B (zh) 葡糖淀粉酶共混物
CN104602540B (zh) 能抵抗与氧化相关的活性损失的里氏木霉葡糖淀粉酶变体及其用途
JP2016019522A (ja) グルコアミラーゼの変異体
EP2588602A1 (en) Variants of glucoamylase