IN2015DN00231A - - Google Patents

Download PDF

Info

Publication number
IN2015DN00231A
IN2015DN00231A IN231DEN2015A IN2015DN00231A IN 2015DN00231 A IN2015DN00231 A IN 2015DN00231A IN 231DEN2015 A IN231DEN2015 A IN 231DEN2015A IN 2015DN00231 A IN2015DN00231 A IN 2015DN00231A
Authority
IN
India
Prior art keywords
variants
disclosed
glucoamylase
methods
biofuel
Prior art date
Application number
Inventor
Jacob Flyvholm Cramer
Peter Edvard Degn
Igor Nikolaev
Viktor Alekseyev
Casper Willem Vroemen
Pazmino Daniel Torres
Neville Marshall Fish
Original Assignee
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
Publication of IN2015DN00231A publication Critical patent/IN2015DN00231A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E10/00—Energy generation through renewable energy sources
    • Y02E10/30—Energy from the sea, e.g. using wave energy or salinity gradient

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to variants having glucoamylase activity with improved properties and to compositions comprising these variants suitable for use for example in the production of a food beverage (e.g. beer) feed biochemical or biofuel. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. Furthermore different methods and uses related to glucoamylases according to the invention are disclosed such as in a brewing process.
IN231DEN2015 2012-08-22 2013-08-21 IN2015DN00231A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261692040P 2012-08-22 2012-08-22
EP12189588 2012-10-23
PCT/EP2013/067391 WO2014029808A1 (en) 2012-08-22 2013-08-21 Variants having glucoamylase activity

Publications (1)

Publication Number Publication Date
IN2015DN00231A true IN2015DN00231A (en) 2015-06-12

Family

ID=47046486

Family Applications (1)

Application Number Title Priority Date Filing Date
IN231DEN2015 IN2015DN00231A (en) 2012-08-22 2013-08-21

Country Status (9)

Country Link
US (2) US20150315558A1 (en)
EP (1) EP2888357A1 (en)
JP (1) JP2015526090A (en)
CN (1) CN104619836A (en)
AU (1) AU2013305007A1 (en)
EA (1) EA201590425A1 (en)
IN (1) IN2015DN00231A (en)
MX (1) MX2015002099A (en)
WO (1) WO2014029808A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112016018075B1 (en) * 2014-02-07 2022-01-18 Novozymes A/S COMPOSITION COMPRISING AN ALPHA-AMYLASE, A PUULULANASE AND A GLYCOAMYLASE ENZYME AND A METHOD OF MANUFACTURING GLUCOSE SYRUP FROM LIQUEFIED STARCH
US20170362621A1 (en) 2014-12-18 2017-12-21 Danisco Us Inc. Engineered multifunctional enzymes and methods of use
US20180002683A1 (en) 2014-12-18 2018-01-04 Danisco Us Inc. Engineered multifunctional enzymes and methods of use
CN107250163A (en) 2015-02-06 2017-10-13 嘉吉公司 The glucoamylase of modification and the yeast strain produced with enhanced biologic
CN107429220A (en) 2015-03-27 2017-12-01 嘉吉公司 Yeast strain through glucose starch enzyme modification and the method for producing biologic
JP6849316B2 (en) * 2016-04-26 2021-03-24 サッポロビール株式会社 Manufacturing method of beer-taste beverage and beer-taste beverage
BR112019002238A2 (en) 2016-08-05 2019-05-14 Cargill, Incorporated manipulated polypeptide and cell, and fermentation method.
US11634673B2 (en) 2017-09-29 2023-04-25 Dupont Nutrition Biosciences Aps Production of brewer's wort having increase fermentable sugars for fermentation
JP7115830B2 (en) * 2017-09-29 2022-08-09 群栄化学工業株式会社 Method for producing wort or malt extract or brewed liquor
CN112218953B (en) 2018-03-09 2024-10-01 丹尼斯科美国公司 Glucoamylase and methods of use thereof
WO2019186371A1 (en) * 2018-03-26 2019-10-03 Lallemand Hungary Liquidity Management Llc Chimeric amylases comprising an heterologous starch binding domain
CN109266633A (en) * 2018-09-14 2019-01-25 华东理工大学 Carbohydrate binding module and its application in immobilised enzymes preparation and fusion protein purification
JP7096290B2 (en) * 2020-06-12 2022-07-05 サッポロビール株式会社 Beer taste beverage
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN112662648B (en) * 2021-01-04 2022-09-06 杭州荔枝红生物科技有限公司 Xylanase GH11-xylanase mutant and application thereof

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
GB2089836B (en) 1980-12-16 1984-06-20 Suntory Ltd Process for producing alcohol by fermentation without cooking
DK252483D0 (en) 1983-06-03 1983-06-03 Novo Industri As ALPHA-ACETOLACTATE DECARBOXYLASEEMZYM PRODUCT AND PREPARATION thereof
US4794175A (en) 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
US4666718A (en) 1984-08-15 1987-05-19 Miller Brewing Company Preparation of low calorie beer
US4618579A (en) 1984-09-28 1986-10-21 Genencor, Inc. Raw starch saccharification
JPH0630586B2 (en) 1984-12-15 1994-04-27 サントリー株式会社 Glucoamylase gene
DE3650202T3 (en) 1985-08-29 2004-06-03 Genencor International, Inc., South San Francisco Expression of heterologous polypeptides in filamentous fungi, methods for their production and vectors for their production.
US5024941A (en) 1985-12-18 1991-06-18 Biotechnica International, Inc. Expression and secretion vector for yeast containing a glucoamylase signal sequence
DK122686D0 (en) 1986-03-17 1986-03-17 Novo Industri As PREPARATION OF PROTEINS
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
ATE125815T1 (en) 1987-06-06 1995-08-15 Omnigene Inc EXPRESSION VECTOR FOR YEAST.
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
ES2170748T3 (en) 1990-10-05 2002-08-16 Genencor Int PROCEDURES FOR THE TREATMENT OF FABRICS CONTAINING COTTON WITH CELL.
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
WO1992010581A1 (en) 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5744716A (en) 1995-06-08 1998-04-28 Scp Global Technologies, A Division Of Preco, Inc. Fluid displacement level, density and concentration measurement system
US6255084B1 (en) 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
US6352851B1 (en) * 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6254914B1 (en) 1999-07-02 2001-07-03 The Board Of Trustees Of The University Of Illinois Process for recovery of corn coarse fiber (pericarp)
DK1309677T4 (en) 2000-08-11 2012-06-25 Genencor Int Bac illustration transformation, transformants and mutant libraries
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
GB0129864D0 (en) 2001-12-13 2002-02-06 Danisco Animal feed
EP1604019B1 (en) 2003-03-10 2010-01-06 Novozymes A/S Alcohol product processes
EP2534961A1 (en) 2003-03-10 2012-12-19 POET Research, Inc. Method for producing ethanol using raw starch
WO2005001036A2 (en) 2003-05-29 2005-01-06 Genencor International, Inc. Novel trichoderma genes
US6899910B2 (en) 2003-06-12 2005-05-31 The United States Of America As Represented By The Secretary Of Agriculture Processes for recovery of corn germ and optionally corn coarse fiber (pericarp)
CA2546659C (en) 2003-11-21 2014-02-18 Genencor International, Inc. Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch substrates
US7037704B2 (en) 2004-05-27 2006-05-02 Genencor International, Inc. Heterologous expression of an Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
AU2005310284B2 (en) 2004-11-30 2011-05-19 Genencor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
WO2006073839A2 (en) 2004-12-30 2006-07-13 Genencor International, Inc. Acid fungal proteases
WO2007101888A2 (en) 2006-07-13 2007-09-13 Dsm Ip Assets B.V. Improved brewing process
US9447397B2 (en) 2006-10-10 2016-09-20 Danisco Us Inc. Glucoamylase variants with altered properties
DE102007032649A1 (en) 2007-07-13 2009-01-15 Man Nutzfahrzeuge Ag Tub and method for its manufacture
JP5463292B2 (en) 2007-10-09 2014-04-09 ダニスコ・ユーエス・インク Glucoamylase mutant
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
HUE050328T2 (en) 2007-11-20 2020-11-30 Danisco Us Inc Glucoamylase variants with altered properties
MX2011012841A (en) 2009-06-03 2012-01-27 Danisco Inc Cellulase variants with improved expression, activity and/or stability, and use thereof.
WO2011022465A1 (en) * 2009-08-19 2011-02-24 Danisco Us Inc. Combinatorial variants of glucoamylase with improved specific activity and/or thermostability
JP5827227B2 (en) 2009-08-19 2015-12-02 デュポン ニュートリション バイオサイエンシーズ エーピーエスＤｕＰｏｎｔ Ｎｕｔｒｉｔｉｏｎ Ｂｉｏｓｃｉｅｎｃｅｓ ＡｐＳ Variant of glucoamylase

Also Published As

Publication number Publication date
US20170306309A1 (en) 2017-10-26
EP2888357A1 (en) 2015-07-01
JP2015526090A (en) 2015-09-10
AU2013305007A1 (en) 2015-01-29
CN104619836A (en) 2015-05-13
MX2015002099A (en) 2015-05-11
EA201590425A1 (en) 2015-06-30
US20150315558A1 (en) 2015-11-05
WO2014029808A1 (en) 2014-02-27

Similar Documents

Publication Publication Date Title
IN2015DN00231A (en)
MX337985B (en) Polypeptides having cellobiohydrolase activity and polynucleotides encoding same.
MX2015001203A (en) Enzymes.
MX337919B (en) Polypeptides having cellobiohydrolase activity and polynucleotides encoding same.
EA201270286A1 (en) GLUCOAMILASE OPTIONS
MX353621B (en) Alpha-amylase variants.
MX337913B (en) Polypeptides having cellobiohydrolase activity and polynucleotides encoding same.
IN2014CN04586A (en)
WO2011044279A3 (en) Microbial systems for producing commodity chemicals
MX2012006096A (en) Polypeptides having glucoamylase activity and polynucleotides encoding same.
MX351762B (en) Glucoamylase variants and polynucleotides encoding same.
MX2014012810A (en) Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases.
MX2014003212A (en) Microorganisms and methods for producing alkenes.
MX2014004598A (en) Alpha-amylase variants and polynucleotides encoding same.
MX2015000312A (en) Polypeptides having lipase activity and polynucleotides encoding same.
MX354704B (en) Alpha-amylase variants and polynucleotides encoding same.
EA201790153A3 (en) ENZYME
MX2012006095A (en) Polypeptides having glucoamylase activity and polynucleotides encoding same.
MX2014001594A (en) Polypeptides having cellulase activity and polynucleotides encoding same.
MX2019007766A (en) Enzyme variants with improved ester synthase properties.
WO2013019780A3 (en) Polypeptides having endoglucanase activity and polynucleotides encoding same
MX349517B (en) Polypeptides having protease activity and polynucleotides encoding same.
EP2831234A4 (en) Compositions, methods, and plant genes for the improved production of fermentable sugars for biofuel production
WO2012177983A3 (en) Microorganisms for producing ethylene glycol and methods related thereto
MX2014003403A (en) Polypeptides having protease activity and polynucleotides encoding same.