JP2015521030A5 - - Google Patents
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- JP2015521030A5 JP2015521030A5 JP2015505831A JP2015505831A JP2015521030A5 JP 2015521030 A5 JP2015521030 A5 JP 2015521030A5 JP 2015505831 A JP2015505831 A JP 2015505831A JP 2015505831 A JP2015505831 A JP 2015505831A JP 2015521030 A5 JP2015521030 A5 JP 2015521030A5
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- 150000001875 compounds Chemical class 0.000 claims description 202
- 239000011148 porous material Substances 0.000 claims description 116
- 125000003729 nucleotide group Chemical group 0.000 claims description 87
- 108020004414 DNA Proteins 0.000 claims description 63
- 238000000034 method Methods 0.000 claims description 61
- 102000053602 DNA Human genes 0.000 claims description 53
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 46
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 42
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- 239000008151 electrolyte solution Substances 0.000 claims description 31
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- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 28
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 28
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 26
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- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 22
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 22
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- 229920000388 Polyphosphate Polymers 0.000 claims description 15
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- 235000011176 polyphosphates Nutrition 0.000 claims description 15
- 229930024421 Adenine Natural products 0.000 claims description 13
- 229960000643 adenine Drugs 0.000 claims description 13
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 13
- 229940104302 cytosine Drugs 0.000 claims description 13
- 125000003342 alkenyl group Chemical group 0.000 claims description 12
- 125000000217 alkyl group Chemical group 0.000 claims description 12
- 125000000304 alkynyl group Chemical group 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- IVRMZWNICZWHMI-UHFFFAOYSA-N azide group Chemical group [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 claims description 12
- 150000001720 carbohydrates Chemical class 0.000 claims description 12
- 125000004093 cyano group Chemical group *C#N 0.000 claims description 12
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 12
- 229930195729 fatty acid Natural products 0.000 claims description 12
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- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 12
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 11
- 229920000137 polyphosphoric acid Polymers 0.000 claims description 11
- 229940113082 thymine Drugs 0.000 claims description 11
- 229940035893 uracil Drugs 0.000 claims description 11
- 238000007476 Maximum Likelihood Methods 0.000 claims description 9
- 238000010276 construction Methods 0.000 claims description 6
- 239000002243 precursor Substances 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- 238000006555 catalytic reaction Methods 0.000 claims description 4
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- 150000007523 nucleic acids Chemical group 0.000 claims description 4
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- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 101710092462 Alpha-hemolysin Proteins 0.000 claims description 2
- 239000004475 Arginine Substances 0.000 claims description 2
- 229940126062 Compound A Drugs 0.000 claims description 2
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 claims description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004472 Lysine Substances 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 239000003792 electrolyte Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 239000003228 hemolysin Substances 0.000 claims description 2
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 235000021317 phosphate Nutrition 0.000 claims description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 2
- 230000000379 polymerizing effect Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- -1 compound Compound Chemical class 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 27
- 150000002500 ions Chemical class 0.000 description 5
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 159000000032 aromatic acids Chemical class 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 229960000956 coumarin Drugs 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261621981P | 2012-04-09 | 2012-04-09 | |
| US61/621,981 | 2012-04-09 | ||
| US201261662330P | 2012-06-20 | 2012-06-20 | |
| US201261662334P | 2012-06-20 | 2012-06-20 | |
| US201261662329P | 2012-06-20 | 2012-06-20 | |
| US61/662,330 | 2012-06-20 | ||
| US61/662,329 | 2012-06-20 | ||
| US61/662,334 | 2012-06-20 | ||
| US201361781353P | 2013-03-14 | 2013-03-14 | |
| US61/781,353 | 2013-03-14 | ||
| PCT/US2013/035635 WO2013154999A2 (en) | 2012-04-09 | 2013-04-08 | Method of preparation of nanopore and uses thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015521030A JP2015521030A (ja) | 2015-07-27 |
| JP2015521030A5 true JP2015521030A5 (https=) | 2016-06-09 |
| JP6456816B2 JP6456816B2 (ja) | 2019-01-23 |
Family
ID=49328273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015505831A Expired - Fee Related JP6456816B2 (ja) | 2012-04-09 | 2013-04-08 | ナノ細孔の調製方法およびその使用 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10246479B2 (https=) |
| EP (1) | EP2836604B1 (https=) |
| JP (1) | JP6456816B2 (https=) |
| CN (2) | CN104379761B (https=) |
| CA (1) | CA2869753A1 (https=) |
| ES (1) | ES2906186T3 (https=) |
| WO (1) | WO2013154999A2 (https=) |
Families Citing this family (113)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
| JP2004510433A (ja) | 2000-10-06 | 2004-04-08 | ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク | Ｄｎａおよびｒｎａを解読するための大量並行方法 |
| WO2007002204A2 (en) | 2005-06-21 | 2007-01-04 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compostions |
| US7982029B2 (en) | 2005-10-31 | 2011-07-19 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods |
| WO2007053719A2 (en) | 2005-10-31 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
| US8889348B2 (en) | 2006-06-07 | 2014-11-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by nanopore using modified nucleotides |
| WO2008069973A2 (en) | 2006-12-01 | 2008-06-12 | The Trustees Of Columbia University In The City Of New York | Four-color dna sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
| DK2725107T3 (da) | 2007-10-19 | 2019-01-02 | Univ Columbia | DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer |
| WO2009051807A1 (en) | 2007-10-19 | 2009-04-23 | The Trustees Of Columbia University In The City Of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
| US9194838B2 (en) | 2010-03-03 | 2015-11-24 | Osaka University | Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide |
| EP2652153B1 (en) | 2010-12-17 | 2017-07-05 | The Trustees of Columbia University in the City of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
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| WO2013154999A2 (en) | 2012-04-09 | 2013-10-17 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
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| EP3674412A1 (en) | 2012-06-20 | 2020-07-01 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
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