JP2015514404A5 - - Google Patents
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- Publication number
- JP2015514404A5 JP2015514404A5 JP2015506048A JP2015506048A JP2015514404A5 JP 2015514404 A5 JP2015514404 A5 JP 2015514404A5 JP 2015506048 A JP2015506048 A JP 2015506048A JP 2015506048 A JP2015506048 A JP 2015506048A JP 2015514404 A5 JP2015514404 A5 JP 2015514404A5
- Authority
- JP
- Japan
- Prior art keywords
- polynucleotide
- birds
- embryo
- germ cells
- transfection reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 21
- 241000271566 Aves Species 0.000 claims 15
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 210000001161 mammalian embryo Anatomy 0.000 claims 10
- 210000004602 germ cell Anatomy 0.000 claims 9
- 239000012096 transfection reagent Substances 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 5
- 238000001890 transfection Methods 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
- PFNFFQXMRSDOHW-UHFFFAOYSA-N Spermine Natural products NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims 3
- 210000004204 blood vessel Anatomy 0.000 claims 3
- 230000013011 mating Effects 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- KSXTUUUQYQYKCR-LQDDAWAPSA-M 2,3-bis[[(z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC KSXTUUUQYQYKCR-LQDDAWAPSA-M 0.000 claims 2
- QPYUGOUSOOCGGQ-UHFFFAOYSA-N CN(CCCN(CCCCN(CCC(N(CCCCCCCCCCCC)CCCCCCCCCCCC)(CCCCCCCCCCCC)CCCCCCCCCCCC)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(CCCCCCCCCCCC)CCCCCCCCCCCC)(CCCCCCCCCCCC)CCCCCCCCCCCC)C)C)C QPYUGOUSOOCGGQ-UHFFFAOYSA-N 0.000 claims 2
- 125000002091 cationic group Chemical group 0.000 claims 2
- -1 cationic lipid Chemical class 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 229940063675 spermine Drugs 0.000 claims 2
- 230000029812 viral genome replication Effects 0.000 claims 2
- LDGWQMRUWMSZIU-LQDDAWAPSA-M 2,3-bis[(z)-octadec-9-enoxy]propyl-trimethylazanium;chloride Chemical group [Cl-].CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC\C=C/CCCCCCCC LDGWQMRUWMSZIU-LQDDAWAPSA-M 0.000 claims 1
- WALUVDCNGPQPOD-UHFFFAOYSA-M 2,3-di(tetradecoxy)propyl-(2-hydroxyethyl)-dimethylazanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCOCC(C[N+](C)(C)CCO)OCCCCCCCCCCCCCC WALUVDCNGPQPOD-UHFFFAOYSA-M 0.000 claims 1
- 241000272525 Anas platyrhynchos Species 0.000 claims 1
- 241000272814 Anser sp. Species 0.000 claims 1
- RBGCWNFXPQALRC-UHFFFAOYSA-N CN(CCCN(CCCCN(CCC(N(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)(C(CCCCCCCCCCCCCCC)=O)C(CCCCCCCCCCCCCCC)=O)C)C)C RBGCWNFXPQALRC-UHFFFAOYSA-N 0.000 claims 1
- NFIDGYIHSCPMMK-SDLHUVDUSA-N CN(CCCN(CCCCN(CCC(N(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)C)C)C Chemical compound CN(CCCN(CCCCN(CCC(N(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)(CCCCCCCC\C=C/CCCCCCCC)CCCCCCCC\C=C/CCCCCCCC)C)C)C NFIDGYIHSCPMMK-SDLHUVDUSA-N 0.000 claims 1
- 108091033409 CRISPR Proteins 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- XULFJDKZVHTRLG-JDVCJPALSA-N DOSPA trifluoroacetate Chemical group [O-]C(=O)C(F)(F)F.CCCCCCCC\C=C/CCCCCCCCOCC(C[N+](C)(C)CCNC(=O)C(CCCNCCCN)NCCCN)OCCCCCCCC\C=C/CCCCCCCC XULFJDKZVHTRLG-JDVCJPALSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 241000287828 Gallus gallus Species 0.000 claims 1
- 241000286209 Phasianidae Species 0.000 claims 1
- 108091027967 Small hairpin RNA Proteins 0.000 claims 1
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- 238000010459 TALEN Methods 0.000 claims 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 1
- DTQVDTLACAAQTR-UHFFFAOYSA-M Trifluoroacetate Chemical compound [O-]C(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-M 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 1
- 230000000840 anti-viral effect Effects 0.000 claims 1
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 claims 1
- PSLWZOIUBRXAQW-UHFFFAOYSA-M dimethyl(dioctadecyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC PSLWZOIUBRXAQW-UHFFFAOYSA-M 0.000 claims 1
- 210000003278 egg shell Anatomy 0.000 claims 1
- 210000002257 embryonic structure Anatomy 0.000 claims 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 125000002811 oleoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C([H])=C([H])\C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 239000004055 small Interfering RNA Substances 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261636331P | 2012-04-20 | 2012-04-20 | |
| US61/636,331 | 2012-04-20 | ||
| US201361783823P | 2013-03-14 | 2013-03-14 | |
| US61/783,823 | 2013-03-14 | ||
| AU2013204327 | 2013-04-12 | ||
| AU2013204327A AU2013204327B2 (en) | 2012-04-20 | 2013-04-12 | Cell transfection method |
| PCT/AU2013/000414 WO2013155572A1 (en) | 2012-04-20 | 2013-04-19 | Cell transfection method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015514404A JP2015514404A (ja) | 2015-05-21 |
| JP2015514404A5 true JP2015514404A5 (https=) | 2016-06-16 |
| JP6531239B2 JP6531239B2 (ja) | 2019-06-19 |
Family
ID=49515073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015506048A Active JP6531239B2 (ja) | 2012-04-20 | 2013-04-19 | 細胞トランスフェクション法 |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US10897881B2 (https=) |
| EP (2) | EP3460065B1 (https=) |
| JP (1) | JP6531239B2 (https=) |
| KR (1) | KR102076784B1 (https=) |
| CN (2) | CN104619848B (https=) |
| AU (2) | AU2013204327B2 (https=) |
| BR (1) | BR112014026186B8 (https=) |
| ES (2) | ES2963732T3 (https=) |
| WO (1) | WO2013155572A1 (https=) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| AU2013204327B2 (en) | 2012-04-20 | 2016-09-01 | Aviagen | Cell transfection method |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| CN105101951B (zh) * | 2012-10-29 | 2021-08-03 | 新加坡科技研究局 | 一种用于基因-药物治疗的新型试剂 |
| EP3363902B1 (en) | 2012-12-06 | 2019-11-27 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
| WO2014093709A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| DK2931897T3 (en) | 2012-12-12 | 2018-02-05 | Broad Inst Inc | CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS |
| EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| MX374090B (es) | 2013-03-14 | 2025-03-05 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico. |
| KR20250012194A (ko) | 2013-06-17 | 2025-01-23 | 더 브로드 인스티튜트, 인코퍼레이티드 | 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용 |
| MX2015017312A (es) | 2013-06-17 | 2017-04-10 | Broad Inst Inc | Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas. |
| WO2014204724A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| EP3725885A1 (en) | 2013-06-17 | 2020-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| EP3011030B1 (en) | 2013-06-17 | 2023-11-08 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| JP6793547B2 (ja) | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物 |
| BR112016013207A2 (pt) | 2013-12-12 | 2017-09-26 | Massachusetts Inst Technology | administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para o hbv e distúrbios e doenças virais |
| EP3080259B1 (en) | 2013-12-12 | 2023-02-01 | The Broad Institute, Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
| WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
| EP3470089A1 (en) * | 2013-12-12 | 2019-04-17 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
| MX2016007325A (es) | 2013-12-12 | 2017-07-19 | Broad Inst Inc | Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos. |
| AU2014361781B2 (en) | 2013-12-12 | 2021-04-01 | Massachusetts Institute Of Technology | Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing |
| CN103782953A (zh) * | 2014-01-23 | 2014-05-14 | 固镇县玉鹏蔬菜开发有限责任公司 | 一种小鹅瘟的预防方法 |
| CN105265383A (zh) * | 2014-10-18 | 2016-01-27 | 颍上县永祥旱粮研究所 | 一种林下鸡养殖方法 |
| EP3889260A1 (en) | 2014-12-12 | 2021-10-06 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
| WO2016106244A1 (en) | 2014-12-24 | 2016-06-30 | The Broad Institute Inc. | Crispr having or associated with destabilization domains |
| CN104789599A (zh) * | 2015-04-13 | 2015-07-22 | 浙江省农业科学院 | 一种提高慢病毒胚盘下腔注射制备转基因家禽效率的方法 |
| TWI813532B (zh) | 2015-06-18 | 2023-09-01 | 美商博得學院股份有限公司 | 降低脱靶效應的ｃｒｉｓｐｒ酶突變 |
| WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| WO2017024343A1 (en) * | 2015-08-07 | 2017-02-16 | Commonwealth Scientific And Industrial Research Organisation | Method for producing an animal comprising a germline genetic modification |
| EP3380611A4 (en) | 2015-11-24 | 2020-02-12 | Commonwealth Scientific and Industrial Research Organisation | PRODUCTION OF VIRUSES IN A CELL CULTURE |
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| WO2017111144A1 (ja) * | 2015-12-25 | 2017-06-29 | 国立研究開発法人産業技術総合研究所 | 遺伝子改変家禽卵 |
| EP3629719B1 (en) * | 2017-05-25 | 2023-08-23 | Eggxyt Ltd | Methods for gender determination of avian embryos in unhatched eggs and means thereof |
| CA3065317A1 (en) | 2017-05-31 | 2018-12-06 | Commonwealth Scientific And Industrial Research Organisation | Trait selection in avians |
| CN109275622A (zh) * | 2017-07-20 | 2019-01-29 | 龚海华 | 一种蛋鸡的循环养殖方法 |
| CN107723313A (zh) * | 2017-10-30 | 2018-02-23 | 扬州大学 | 一种外源物质导入鸡胚的方法 |
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| CN118575784B (zh) * | 2024-06-27 | 2025-09-02 | 江苏省家禽科学研究所 | 一种提高肉鸡肌肉营养价值的养殖方法 |
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| BR112018010503A2 (pt) * | 2015-11-24 | 2018-11-13 | Commw Scient Ind Res Org | ovo de ave, método para replicar um vírus, vírus, método para produzir uma composição de vacina, composição de vacina, ave transgênica, e método para produzir uma ave |
| CN105524941A (zh) * | 2016-01-19 | 2016-04-27 | 扬州大学 | 一种特异于禽类原始生殖细胞的基因转移系统及方法 |
| TW202241259A (zh) * | 2020-12-31 | 2022-11-01 | 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） | 不育禽類胚胎、其產生及用途 |
-
2013
- 2013-04-12 AU AU2013204327A patent/AU2013204327B2/en active Active
- 2013-04-19 ES ES18187574T patent/ES2963732T3/es active Active
- 2013-04-19 BR BR112014026186A patent/BR112014026186B8/pt active IP Right Grant
- 2013-04-19 JP JP2015506048A patent/JP6531239B2/ja active Active
- 2013-04-19 ES ES13777737T patent/ES2704155T3/es active Active
- 2013-04-19 US US14/394,712 patent/US10897881B2/en active Active
- 2013-04-19 EP EP18187574.1A patent/EP3460065B1/en active Active
- 2013-04-19 KR KR1020147031173A patent/KR102076784B1/ko active Active
- 2013-04-19 EP EP13777737.1A patent/EP2839016B1/en active Active
- 2013-04-19 WO PCT/AU2013/000414 patent/WO2013155572A1/en not_active Ceased
- 2013-04-19 CN CN201380031734.XA patent/CN104619848B/zh active Active
- 2013-04-19 CN CN202011320159.3A patent/CN112626128B/zh active Active
- 2013-04-19 AU AU2013248945A patent/AU2013248945A1/en not_active Abandoned
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2019
- 2019-04-15 US US16/384,782 patent/US11369096B2/en active Active
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2020
- 2020-12-23 US US17/133,142 patent/US12232488B2/en active Active
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2022
- 2022-06-27 US US17/809,219 patent/US12213466B2/en active Active
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2025
- 2025-01-17 US US19/030,795 patent/US20250151705A1/en active Pending
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