CN112626128B - 细胞转染方法 - Google Patents

细胞转染方法 Download PDF

Info

Publication number
CN112626128B
CN112626128B CN202011320159.3A CN202011320159A CN112626128B CN 112626128 B CN112626128 B CN 112626128B CN 202011320159 A CN202011320159 A CN 202011320159A CN 112626128 B CN112626128 B CN 112626128B
Authority
CN
China
Prior art keywords
avian
embryo
transfection
transfection reagent
germ cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202011320159.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN112626128A (zh
Inventor
S·G·蒂亚克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organization CSIRO
Aviagen Inc
Original Assignee
Commonwealth Scientific and Industrial Research Organization CSIRO
Aviagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO, Aviagen Inc filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Publication of CN112626128A publication Critical patent/CN112626128A/zh
Application granted granted Critical
Publication of CN112626128B publication Critical patent/CN112626128B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A01K2217/058—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function due to expression of inhibitory nucleic acid, e.g. siRNA, antisense
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202011320159.3A 2012-04-20 2013-04-19 细胞转染方法 Active CN112626128B (zh)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261636331P 2012-04-20 2012-04-20
US61/636,331 2012-04-20
US201361783823P 2013-03-14 2013-03-14
US61/783,823 2013-03-14
AU2013204327A AU2013204327B2 (en) 2012-04-20 2013-04-12 Cell transfection method
AU2013204327 2013-04-12
PCT/AU2013/000414 WO2013155572A1 (en) 2012-04-20 2013-04-19 Cell transfection method
CN201380031734.XA CN104619848B (zh) 2012-04-20 2013-04-19 细胞转染方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201380031734.XA Division CN104619848B (zh) 2012-04-20 2013-04-19 细胞转染方法

Publications (2)

Publication Number Publication Date
CN112626128A CN112626128A (zh) 2021-04-09
CN112626128B true CN112626128B (zh) 2025-03-28

Family

ID=49515073

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202011320159.3A Active CN112626128B (zh) 2012-04-20 2013-04-19 细胞转染方法
CN201380031734.XA Active CN104619848B (zh) 2012-04-20 2013-04-19 细胞转染方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201380031734.XA Active CN104619848B (zh) 2012-04-20 2013-04-19 细胞转染方法

Country Status (9)

Country Link
US (5) US10897881B2 (https=)
EP (2) EP3460065B1 (https=)
JP (1) JP6531239B2 (https=)
KR (1) KR102076784B1 (https=)
CN (2) CN112626128B (https=)
AU (2) AU2013204327B2 (https=)
BR (1) BR112014026186B8 (https=)
ES (2) ES2963732T3 (https=)
WO (1) WO2013155572A1 (https=)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
DK2911650T3 (da) 2012-10-29 2019-11-18 Agency Science Tech & Res Hidtil ukendt reagens til gen-lægemiddel-terapeutika
PL3360964T3 (pl) 2012-12-06 2020-03-31 Sigma-Aldrich Co. Llc Modyfikacja i regulacja genomu oparta na crispr
EP3031921B1 (en) 2012-12-12 2025-03-12 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
WO2014093709A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
RU2662932C2 (ru) 2013-03-14 2018-07-31 Карибо Биосайенсиз, Инк. Композиции и способы с участием нуклеиновых кислот, нацеленных на нуклеиновые кислоты
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
EP3725885A1 (en) 2013-06-17 2020-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
WO2014204724A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
MX374532B (es) 2013-06-17 2025-03-06 Broad Inst Inc Suministro, uso y aplicaciones terapéuticas de los sistemas y composiciones crispr-cas, para actuar sobre trastornos y enfermedades utilizando componentes víricos.
RU2716420C2 (ru) 2013-06-17 2020-03-11 Те Брод Инститьют Инк. Доставка и применение систем crispr-cas, векторов и композиций для целенаправленного воздействия и терапии в печени
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
SG10201804975PA (en) 2013-12-12 2018-07-30 Broad Inst Inc Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for HBV and Viral Diseases and Disorders
JP6793547B2 (ja) 2013-12-12 2020-12-02 ザ・ブロード・インスティテュート・インコーポレイテッド 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物
JP7103750B2 (ja) 2013-12-12 2022-07-20 ザ・ブロード・インスティテュート・インコーポレイテッド ゲノム編集のためのＣＲＩＳＰＲ－Ｃａｓ系及び組成物の送達、使用及び治療適用
JP6712948B2 (ja) 2013-12-12 2020-06-24 ザ・ブロード・インスティテュート・インコーポレイテッド 組成物、及びヌクレオチドリピート障害におけるｃｒｉｓｐｒ−ｃａｓ系の使用方法
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
EP3470089A1 (en) * 2013-12-12 2019-04-17 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
CN103782953A (zh) * 2014-01-23 2014-05-14 固镇县玉鹏蔬菜开发有限责任公司 一种小鹅瘟的预防方法
CN105265383A (zh) * 2014-10-18 2016-01-27 颍上县永祥旱粮研究所 一种林下鸡养殖方法
EP3889260A1 (en) 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
CA2970370A1 (en) 2014-12-24 2016-06-30 Massachusetts Institute Of Technology Crispr having or associated with destabilization domains
CN104789599A (zh) * 2015-04-13 2015-07-22 浙江省农业科学院 一种提高慢病毒胚盘下腔注射制备转基因家禽效率的方法
TWI906646B (zh) 2015-06-18 2025-12-01 美商博得學院股份有限公司 降低脫靶效應的ｃｒｉｓｐｒ酶突變
WO2016205759A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
CN108474007A (zh) * 2015-08-07 2018-08-31 联邦科学技术研究组织 产生包含种系遗传修饰的动物的方法
EP3380616A4 (en) 2015-11-24 2019-11-06 Commonwealth Scientific and Industrial Research Organisation MANUFACTURE OF VIRUSES IN BIRDS
MX387420B (es) 2015-11-24 2025-03-18 Commw Scient Ind Res Org Producción de virus en cultivos celulares.
WO2018216022A1 (en) * 2017-05-25 2018-11-29 Eggxyt Ltd Methods for gender determination of avian embryos in unhatched eggs and means thereof
US10858669B2 (en) 2015-12-25 2020-12-08 National Institute Of Advanced Industrial Science And Technology Genetically modified chicken egg with an exogenous sequence knocked into the ovalbumin gene
CA3065317A1 (en) 2017-05-31 2018-12-06 Commonwealth Scientific And Industrial Research Organisation Trait selection in avians
CN109275622A (zh) * 2017-07-20 2019-01-29 龚海华 一种蛋鸡的循环养殖方法
CN107723313A (zh) * 2017-10-30 2018-02-23 扬州大学 一种外源物质导入鸡胚的方法
IL274632B2 (en) * 2017-11-15 2024-12-01 Weird Science Llc Methods and compositions for non-myeloablative bone marrow reconstitution
KR102103103B1 (ko) * 2018-08-16 2020-04-21 (주)라트바이오 인위적 뉴클레아제를 생산하는 형질전환 동물 및 형질전환 배아
AU2020272668B2 (en) * 2019-04-08 2023-07-06 Dna Twopointo Inc. Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias
WO2020210236A1 (en) 2019-04-08 2020-10-15 Dna Twopointo Inc. Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis
US20250327090A1 (en) * 2024-04-23 2025-10-23 TransCytos, LLC Devices and Methods for Transfection
CN118575784B (zh) * 2024-06-27 2025-09-02 江苏省家禽科学研究所 一种提高肉鸡肌肉营养价值的养殖方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104619848A (zh) * 2012-04-20 2015-05-13 联邦科学技术研究组织 细胞转染方法
CN105524941A (zh) * 2016-01-19 2016-04-27 扬州大学 一种特异于禽类原始生殖细胞的基因转移系统及方法
CN107072183A (zh) * 2014-07-14 2017-08-18 华盛顿州立大学 消除种系细胞的nanos敲除
CN108474007A (zh) * 2015-08-07 2018-08-31 联邦科学技术研究组织 产生包含种系遗传修饰的动物的方法
CN108884461A (zh) * 2015-11-24 2018-11-23 联邦科学技术研究组织 在禽蛋中产生病毒
CN110066769A (zh) * 2013-06-05 2019-07-30 皮尔布莱特研究所 用于改善的病毒生产的禽类细胞
CN117545350A (zh) * 2020-12-31 2024-02-09 以色列农业和农村发展部农业研究组织(沃尔卡尼研究所) 不育禽类胚胎、其产生及用途

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162215A (en) * 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
GB8906214D0 (en) 1989-03-17 1989-05-04 Nat Res Dev Introducing an exogenous gene into birds
US5631329A (en) 1990-08-27 1997-05-20 Dendritech, Inc. Process for producing hyper-comb-branched polymers
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5266106A (en) 1992-10-22 1993-11-30 Xerox Corporation Ink compositions with dendrimer grafts
WO1996031549A1 (de) 1995-04-07 1996-10-10 Mueller Egbert Dendrimere pfropfpolymerisate
US20030069173A1 (en) 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
US6251433B1 (en) 1996-08-13 2001-06-26 Chiron Corporation Polycationic polymers
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
DE69831958T2 (de) 1997-08-04 2006-07-27 University of Massachusetts, a Public Institution of Higher Education of The Commonwealth of Massachusetts, Amherst Primordiale keimzellinie aus vögeln und verfahren zu deren langzeitkultivierung
BR9811832A (pt) 1997-08-04 2000-08-15 Univ Massachusetts Processo de cultivo, processo de melhorado de produção de aves quiméricas, e, linhagem de células eg de aves
JP2001520009A (ja) * 1997-10-16 2001-10-30 ユニバーシティ・オブ・ジョージア・リサーチ・ファウンデイション・インコーポレイテッド 鳥の導入遺伝子についてのマグナム特異的プロモーターを含むベクター
ATE378398T1 (de) 1999-02-11 2007-11-15 Hanmi Pharm Ind Co Ltd Pluripotente embryonale keimzellinie aus vögeln
US6140118A (en) 1999-08-11 2000-10-31 Wisconsin Alumni Research Foundation Avian blastodermal cell lines
JP4609869B2 (ja) 1999-12-03 2011-01-12 独立行政法人科学技術振興機構 トランスポゾン転移酵素および遺伝子改変方法
US8202979B2 (en) 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
JP4300287B2 (ja) 2002-02-14 2009-07-22 国立大学法人東京海洋大学 分離始原生殖細胞の移植による生殖細胞系列への分化誘導法
JP4364474B2 (ja) 2002-02-15 2009-11-18 大学共同利用機関法人情報・システム研究機構 哺乳動物において機能的なトランスポゾン
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
KR100502889B1 (ko) 2003-04-21 2005-07-20 한미약품(주) 조류 원시생식세포의 생식선 전이 효율의 개선방법
US20060174362A1 (en) * 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
CA2595576A1 (en) 2005-02-01 2006-08-10 Origen Therapeutics, Inc. Transgenic chickens
AU2006277679B2 (en) * 2005-03-31 2012-08-02 Ronen Kahana Creating poultry and other animals resistant to viral disease
US20070293449A1 (en) * 2006-06-20 2007-12-20 Nastech Pharmaceutical Company Inc. Compositions and methods for delivery of double-stranded rna
WO2008060510A2 (en) * 2006-11-13 2008-05-22 Sangamo Biosciences, Inc. Zinc finger nuclease for targeting the human glucocorticoid receptor locus
NZ581542A (en) * 2007-05-16 2012-02-24 Mat Malta Advanced Technologies Ltd Treatment and prevention of influenza
JP5554231B2 (ja) * 2007-06-13 2014-07-23 コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション トリの生産形質の改変
AU2008286791A1 (en) * 2007-08-14 2009-02-19 Symphogen A/S Transgenic chickens with an inactivated endogenous gene locus
AU2008301229B2 (en) 2007-09-20 2013-10-10 Australian Poultry Crc Pty Ltd Novel avian cytokines and genetic sequences encoding same
JP5940977B2 (ja) 2009-08-11 2016-06-29 サンガモ バイオサイエンシーズ， インコーポレイテッド 標的改変によるホモ接合生物
MX363013B (es) * 2011-02-25 2019-03-04 Recombinetics Inc Animales genéticamente modificados y métodos para su obtención.
WO2014138792A1 (en) * 2013-03-14 2014-09-18 Commonwealth Scientific And Industrial Research Organisation Double-stranded rna
EP4286517A3 (en) * 2013-04-04 2024-03-13 President and Fellows of Harvard College Therapeutic uses of genome editing with crispr/cas systems

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104619848A (zh) * 2012-04-20 2015-05-13 联邦科学技术研究组织 细胞转染方法
CN110066769A (zh) * 2013-06-05 2019-07-30 皮尔布莱特研究所 用于改善的病毒生产的禽类细胞
CN107072183A (zh) * 2014-07-14 2017-08-18 华盛顿州立大学 消除种系细胞的nanos敲除
CN108474007A (zh) * 2015-08-07 2018-08-31 联邦科学技术研究组织 产生包含种系遗传修饰的动物的方法
CN108884461A (zh) * 2015-11-24 2018-11-23 联邦科学技术研究组织 在禽蛋中产生病毒
CN105524941A (zh) * 2016-01-19 2016-04-27 扬州大学 一种特异于禽类原始生殖细胞的基因转移系统及方法
CN117545350A (zh) * 2020-12-31 2024-02-09 以色列农业和农村发展部农业研究组织(沃尔卡尼研究所) 不育禽类胚胎、其产生及用途

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ChIP-Seq技术在研究转录因子调控干细胞分化中的应用;乌云毕力格;顾婷玉;何志颖;吴侠;李光鹏;李前忠;丁小燕;左永春;王欣;;中国细胞生物学学报;20130522(第06期);第130-139页 *
CRISPR/Cas9系统在家禽中应用研究进展;张蕾;张海波;章敬旗;张扬;常国斌;陈国宏;王健;;中国畜牧兽医;20201231(第01期);第156-163页 *
Transgenic chimera quail production by microinjecting lentiviral vector into the blood vessel of the early embryo;SUN，P.et al.;Animal Science Journal;20111208;摘要 *
受体胚龄对鸡原始生殖细胞移植后归巢的影响;陈美娟;陈东阳;谢龙;陆振萍;杨蒙蒙;莫丽芬;卢克焕;陆阳清;;南方农业学报;20160718(第06期);第148-152页 *

Also Published As

Publication number Publication date
JP6531239B2 (ja) 2019-06-19
BR112014026186B8 (pt) 2023-01-31
KR102076784B1 (ko) 2020-02-12
AU2013204327A1 (en) 2013-11-07
JP2015514404A (ja) 2015-05-21
US20210112789A1 (en) 2021-04-22
US20230189769A1 (en) 2023-06-22
US12232488B2 (en) 2025-02-25
US20250151705A1 (en) 2025-05-15
EP3460065A1 (en) 2019-03-27
EP2839016B1 (en) 2018-10-03
AU2013204327B2 (en) 2016-09-01
US11369096B2 (en) 2022-06-28
US12213466B2 (en) 2025-02-04
EP2839016A4 (en) 2015-11-25
BR112014026186B1 (pt) 2022-02-01
CN112626128A (zh) 2021-04-09
ES2704155T3 (es) 2019-03-14
ES2963732T3 (es) 2024-04-01
CN104619848A (zh) 2015-05-13
US10897881B2 (en) 2021-01-26
AU2013248945A1 (en) 2014-10-30
EP3460065B1 (en) 2023-08-23
US20190261609A1 (en) 2019-08-29
KR20150014446A (ko) 2015-02-06
US20150072064A1 (en) 2015-03-12
WO2013155572A1 (en) 2013-10-24
BR112014026186A2 (pt) 2017-07-18
CN104619848B (zh) 2020-12-15
EP2839016A1 (en) 2015-02-25

Similar Documents

Publication Publication Date Title
CN112626128B (zh) 细胞转染方法
US20100105140A1 (en) Plaice dna transposon system
CN108884461A (zh) 在禽蛋中产生病毒
CN105018523B (zh) 一种zb转座子系统及其介导的基因转移方法
US20080113437A1 (en) In-vitro method for producing oocytes or eggs having targeted genomic modification
AU2002354121A1 (en) siRNA Expression System and Method for Producing Functional Gene Knockdown Cell Using the Same
WO2018013759A1 (en) Poultry and offspring sex selection
JP2010526542A (ja) インフルエンザの治療および予防
Jordan et al. Expression of green fluorescent protein in the chicken using in vivo transfection of the piggyBac transposon
CN110891419A (zh) 评价crispr/cas-诱导的与外源供体核酸的体内重组
CN105524941A (zh) 一种特异于禽类原始生殖细胞的基因转移系统及方法
KR20240002710A (ko) 부위 특이적 넉인 시스템 (Site-specific Knock-in(KI)system)을 이용한 형질전환 소 생산 방법
US20260078411A1 (en) Novel genomic safe harbor and use thereof
Kolluri Expression analysis of growth regulatory genes and optimization of CRISPR/Cas9 mediated gene editing of activin receptor type 2B (ACVR2B) in broiler chicken
KR20250047845A (ko) 부위 특이적 넉인 시스템 (Site-specific Knock-in(KI) system)을 이용한 형질전환 소 생산 방법
CN117042600A (zh) 具有经编辑的anp32基因的抗甲型流感动物
Jordan Utilizing the piggyBac transposon in transgenic chicken strategies
Challagulla Precision engineering of the chicken genome for disease control

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TG01 Patent term adjustment

Free format text: NEW EXPIRY DATE: 20330822

TG01 Patent term adjustment